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    Bo Huang, PhD

    TitleAssociate Professor
    SchoolUCSF School of Pharmacy
    DepartmentPharmaceutical Chemistry
    Address1700 4th Street
    San Francisco CA 94158

       Awards and Honors
      2011NIH Director's New Innovator Award
      2010Searle Scholar
      2010Packard Fellowship for Science and Engineering
      2007GE & Science Young Life Scientist Award, North America Regional Winner
      2001 - 2004Stanford Graduate Fellowship
      2006Annual Reviews Prize in Physical Chemistry, Stanford University

      Cellular processes are carried out by coordinated participation of many biomolecules in a tiny volume. Many people have been dreaming to see clear pictures of these processes in order to understand how these molecules work together. Taking on this challenge, we are developing new visualization techniques and imaging probes by combining super-resolution microscopy, protein engineering and microfluidic automation. We are particularly interested in the following problems:

      (1) Physical organization and dynamics of the genome,
      (2) Architecture of large protein complexes such as the centrosome, and
      (3) Spatial distribution of membrane proteins, particularly G-protein coupled receptors and neuron adhesion molecules, and how this distribution defines their signaling specificity.

      In order to study these systems, we are developing the following microscopy technologies:

      (1) Super-resolution and light-sheet microscopes that can visualize subcellular structures at a higher spatial resolution, record long term cell behavior, and track cells in intact animals, and
      (2) New fluorescent probes based on fluorescent proteins, nanobodies and aptamers so that biological questions can be converted into "imageable" ones.

       ORNG Applications 
       In The News
       Awarded Grants

      Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
      List All   |   Timeline
      1. Puchner EM, Huang B, Gaub HE. Single molecule techniques--applications in biology. FEBS Lett. 2014 Oct 1; 588(19):3519.
        View in: PubMed
      2. Wang Y, Schnitzbauer J, Hu Z, Li X, Cheng Y, Huang ZL, Huang B. Localization events-based sample drift correction for localization microscopy with redundant cross-correlation algorithm. Opt Express. 2014 Jun 30; 22(13):15982-91.
        View in: PubMed PMC4162368
      3. McGorty R, Schnitzbauer J, Zhang W, Huang B. Correction of depth-dependent aberrations in 3D single-molecule localization and super-resolution microscopy. Opt Lett. 2014 Jan 15; 39(2):275-8.
        View in: PubMed PMC4030053
      4. Chen B, Huang B. Imaging Genomic Elements in Living Cells Using CRISPR/Cas9. Methods Enzymol. 2014; 546:337-54.
        View in: PubMed
      5. Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang B. Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell. 2013 Dec 19; 155(7):1479-91.
        View in: PubMed PMC3918502
      6. Mennella V, Agard DA, Huang B, Pelletier L. Amorphous no more: subdiffraction view of the pericentriolar material architecture. Trends Cell Biol. 2014 Mar; 24(3):188-97.
        View in: PubMed PMC3991556
      7. Puchner EM, Walter JM, Kasper R, Huang B, Lim WA. Counting molecules in single organelles with superresolution microscopy allows tracking of the endosome maturation trajectory. Proc Natl Acad Sci U S A. 2013 Oct 1; 110(40):16015-20.
        View in: PubMed PMC3791776
      8. Schnitzbauer J, McGorty R, Huang B. 4Pi fluorescence detection and 3D particle localization with a single objective. Opt Express. 2013 Aug 26; 21(17):19701-8.
        View in: PubMed PMC3796685
      9. Huang F, Wang X, Ostertag EM, Nuwal T, Huang B, Jan YN, Basbaum AI, Jan LY. TMEM16C facilitates Na(+)-activated K+ currents in rat sensory neurons and regulates pain processing. Nat Neurosci. 2013 Sep; 16(9):1284-90.
        View in: PubMed PMC4034143
      10. McGorty R, Kamiyama D, Huang B. Active microscope stabilization in three dimensions using image correlation. Optical Nanoscopy. 2013; 2:3.
      11. Irannejad R, Tomshine JC, Tomshine JR, Chevalier M, Mahoney JP, Steyaert J, Rasmussen SG, Sunahara RK, El-Samad H, Huang B, von Zastrow M. Conformational biosensors reveal GPCR signalling from endosomes. Nature. 2013 Mar 28; 495(7442):534-8.
        View in: PubMed PMC3835555
      12. Stender AS, Marchuk K, Liu C, Sander S, Meyer MW, Smith EA, Neupane B, Wang G, Li J, Cheng JX, Huang B, Fang N. Single cell optical imaging and spectroscopy. Chem Rev. 2013 Apr 10; 113(4):2469-527.
        View in: PubMed PMC3624028
      13. Kamiyama D, Huang B. Development in the STORM. Dev Cell. 2012 Dec 11; 23(6):1103-10.
        View in: PubMed PMC3523271
      14. Mennella V, Keszthelyi B, McDonald KL, Chhun B, Kan F, Rogers GC, Huang B, Agard DA. Subdiffraction-resolution fluorescence microscopy reveals a domain of the centrosome critical for pericentriolar material organization. Nat Cell Biol. 2012 Nov; 14(11):1159-68.
        View in: PubMed PMC3767400
      15. Beaudoin GM, Schofield CM, Nuwal T, Zang K, Ullian EM, Huang B, Reichardt LF. Afadin, a Ras/Rap effector that controls cadherin function, promotes spine and excitatory synapse density in the hippocampus. J Neurosci. 2012 Jan 4; 32(1):99-110.
        View in: PubMed PMC3305287
      16. Kasper R, Huang B. SnapShot: Light microscopy. Cell. 2011 Nov 23; 147(5):1198.e1.
        View in: PubMed
      17. Huang B, Babcock H, Zhuang X. Breaking the diffraction barrier: super-resolution imaging of cells. Cell. 2010 Dec 23; 143(7):1047-58.
        View in: PubMed PMC3272504
      18. Dani A, Huang B, Bergan J, Dulac C, Zhuang X. Superresolution imaging of chemical synapses in the brain. Neuron. 2010 Dec 9; 68(5):843-56.
        View in: PubMed PMC3057101
      19. Wu M, Huang B, Graham M, Raimondi A, Heuser JE, Zhuang X, De Camilli P. Coupling between clathrin-dependent endocytic budding and F-BAR-dependent tubulation in a cell-free system. Nat Cell Biol. 2010 Sep; 12(9):902-8.
        View in: PubMed PMC3338250
      20. Dani A, Huang B. New resolving power for light microscopy: applications to neurobiology. Curr Opin Neurobiol. 2010 Oct; 20(5):648-52.
        View in: PubMed
      21. Huang B. Super-resolution optical microscopy: multiple choices. Curr Opin Chem Biol. 2010 Feb; 14(1):10-4.
        View in: PubMed
      22. Huang B, Bates M, Zhuang X. Super-resolution fluorescence microscopy. Annu Rev Biochem. 2009; 78:993-1016.
        View in: PubMed PMC2835776
      23. Huang B, Jones SA, Brandenburg B, Zhuang X. Whole-cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution. Nat Methods. 2008 Dec; 5(12):1047-52.
        View in: PubMed PMC2596623
      24. Huang B, Wang W, Bates M, Zhuang X. Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy. Science. 2008 Feb 8; 319(5864):810-3.
        View in: PubMed PMC2633023
      25. Kim S, Huang B, Zare RN. Microfluidic separation and capture of analytes for single-molecule spectroscopy. Lab Chip. 2007 Dec; 7(12):1663-5.
        View in: PubMed
      26. Huang B, Kim S, Wu H, Zare RN. Use of a mixture of n-dodecyl-beta-D-maltoside and sodium dodecyl sulfate in poly(dimethylsiloxane) microchips to suppress adhesion and promote separation of proteins. Anal Chem. 2007 Dec 1; 79(23):9145-9.
        View in: PubMed
      27. Bates M, Huang B, Dempsey GT, Zhuang X. Multicolor super-resolution imaging with photo-switchable fluorescent probes. Science. 2007 Sep 21; 317(5845):1749-53.
        View in: PubMed PMC2633025
      28. Whorton MR, Bokoch MP, Rasmussen SG, Huang B, Zare RN, Kobilka B, Sunahara RK. A monomeric G protein-coupled receptor isolated in a high-density lipoprotein particle efficiently activates its G protein. Proc Natl Acad Sci U S A. 2007 May 1; 104(18):7682-7.
        View in: PubMed PMC1863461
      29. Huang B, Yu F, Zare RN. Surface plasmon resonance imaging using a high numerical aperture microscope objective. Anal Chem. 2007 Apr 1; 79(7):2979-83.
        View in: PubMed
      30. Huang B, Wu H, Bhaya D, Grossman A, Granier S, Kobilka BK, Zare RN. Counting low-copy number proteins in a single cell. Science. 2007 Jan 5; 315(5808):81-4.
        View in: PubMed
      31. Luo Y, Huang B, Wu H, Zare RN. Controlling electroosmotic flow in poly(dimethylsiloxane) separation channels by means of prepolymer additives. Anal Chem. 2006 Jul 1; 78(13):4588-92.
        View in: PubMed
      32. Wu H, Huang B, Zare RN. Generation of complex, static solution gradients in microfluidic channels. J Am Chem Soc. 2006 Apr 5; 128(13):4194-5.
        View in: PubMed
      33. Huang B, Wu H, Kim S, Kobilka BK, Zare RN. Phospholipid biotinylation of polydimethylsiloxane (PDMS) for protein immobilization. Lab Chip. 2006 Mar; 6(3):369-73.
        View in: PubMed
      34. Wu H, Huang B, Zare RN. Construction of microfluidic chips using polydimethylsiloxane for adhesive bonding. Lab Chip. 2005 Dec; 5(12):1393-8.
        View in: PubMed
      35. Huang B, Wu H, Kim S, Zare RN. Coating of poly(dimethylsiloxane) with n-dodecyl-beta-D-maltoside to minimize nonspecific protein adsorption. Lab Chip. 2005 Oct; 5(10):1005-7.
        View in: PubMed
      36. Perroud TD, Huang B, Zare RN. Effect of bin time on the photon counting histogram for one-photon excitation. Chemphyschem. 2005 May; 6(5):905-12.
        View in: PubMed
      37. Huang B, Perroud TD, Zare RN. Photon counting histogram: one-photon excitation. Chemphyschem. 2004 Oct 18; 5(10):1523-31.
        View in: PubMed
      38. Perroud TD, Huang B, Wallace MI, Zare RN. Photon counting histogram for one-photon excitation. Chemphyschem. 2003 Oct 17; 4(10):1121-3.
        View in: PubMed
      39. Whelan RJ, Wohland T, Neumann L, Huang B, Kobilka BK, Zare RN. Analysis of biomolecular interactions using a miniaturized surface plasmon resonance sensor. Anal Chem. 2002 Sep 1; 74(17):4570-6.
        View in: PubMed
      40. Ying L, Huang X, Huang B, Xie J, Zhao J, Zhao XS. Fluorescence emission and absorption spectra of single Anabaena sp. strain PCC7120 cells. Photochem Photobiol. 2002 Sep; 76(3):310-3.
        View in: PubMed
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