 Bo Huang, PhD
|
| Title | Assistant Professor |
|---|
| School | UCSF School of Pharmacy |
|---|
| Department | Pharmaceutical Chemistry |
|---|
| Address | 1700 4th St, UCSF-QB3
San Francisco CA 94158
|
|---|
| Phone | 415-476-1866 |
|---|
| Email | bo.huang@ucsf.edu |
|---|
|
|
 Biography | 2011 | | NIH Director's New Innovator Award | | 2010 | | Searle Scholar | | 2010 | | Packard Fellowship for Science and Engineering | | 2007 | | GE & Science Young Life Scientist Award, North America Regional Winner | | 2001
-
| 2004 | Stanford Graduate Fellowship |
Overview The vast majority of biological processes inside a cell are carried out by the coordinated participation of many proteins. Our research focuses on understanding the mechanisms that enable them to work together. By developing and applying new approaches based on super-resolution microscopy and single-molecule imaging, we are interested in answering three questions: (1) how proteins organize into large complexes such as the nuclear pore complex and centrosome, (2) how proteins dynamically interact with each other to regulate cell signaling, especially in the G-protein coupled receptor pathway, and for cell adhesion molecules to control the development of the nervous system, and (3) how protein and DNA interact each other in the nucleus. We are also interested in combining synthetic chemistry, protein engineering and microfluidics-based high-throughput screening to create new fluorescent probes that allow us to better study these cellular structures and processes.
Bibliographic
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications.
Researchers can login to make corrections and additions, or contact us for help.
-
McGorty R, Kamiyama D, Huang B. Active microscope stabilization in three dimensions using image correlation. Optical Nanoscopy. 2013; 2:3.
-
Irannejad R, Tomshine JC, Tomshine JR, Chevalier M, Mahoney JP, Steyaert J, Rasmussen SG, Sunahara RK, El-Samad H, Huang B, von Zastrow M. Conformational biosensors reveal GPCR signalling from endosomes. Nature. 2013 Mar 28; 495(7442):534-8.
-
Stender AS, Marchuk K, Liu C, Sander S, Meyer MW, Smith EA, Neupane B, Wang G, Li J, Cheng JX, Huang B, Fang N. Single cell optical imaging and spectroscopy. Chem Rev. 2013 Apr 10; 113(4):2469-527.
-
Kamiyama D, Huang B. Development in the STORM. Dev Cell. 2012 Dec 11; 23(6):1103-10.
-
Mennella V, Keszthelyi B, McDonald KL, Chhun B, Kan F, Rogers GC, Huang B, Agard DA. Subdiffraction-resolution fluorescence microscopy reveals a domain of the centrosome critical for pericentriolar material organization. Nat Cell Biol. 2012 Nov; 14(11):1159-68.
-
Shim SH, Xia C, Zhong G, Babcock HP, Vaughan JC, Huang B, Wang X, Xu C, Bi GQ, Zhuang X. Super-resolution fluorescence imaging of organelles in live cells with photoswitchable membrane probes. Proc Natl Acad Sci U S A. 2012 Aug 28; 109(35):13978-83.
-
Zhu L, Zhang W, Elnatan D, Huang B. Faster STORM using compressed sensing. Nat Methods. 2012 Jul; 9(7):721-3.
-
Beaudoin GM, Schofield CM, Nuwal T, Zang K, Ullian EM, Huang B, Reichardt LF. Afadin, a Ras/Rap effector that controls cadherin function, promotes spine and excitatory synapse density in the hippocampus. J Neurosci. 2012 Jan 4; 32(1):99-110.
-
Kasper R, Huang B. SnapShot: Light microscopy. Cell. 2011 Nov 23; 147(5):1198.e1.
-
Huang B. An in-depth view. Nat Methods. 2011 Apr; 8(4):304-5.
-
Huang B, Babcock H, Zhuang X. Breaking the diffraction barrier: super-resolution imaging of cells. Cell. 2010 Dec 23; 143(7):1047-58.
-
Dani A, Huang B, Bergan J, Dulac C, Zhuang X. Superresolution imaging of chemical synapses in the brain. Neuron. 2010 Dec 9; 68(5):843-56.
-
Wu M, Huang B, Graham M, Raimondi A, Heuser JE, Zhuang X, De Camilli P. Coupling between clathrin-dependent endocytic budding and F-BAR-dependent tubulation in a cell-free system. Nat Cell Biol. 2010 Sep; 12(9):902-8.
-
Dani A, Huang B. New resolving power for light microscopy: applications to neurobiology. Curr Opin Neurobiol. 2010 Oct; 20(5):648-52.
-
Huang B. Super-resolution optical microscopy: multiple choices. Curr Opin Chem Biol. 2010 Feb; 14(1):10-4.
-
Huang B, Bates M, Zhuang X. Super-resolution fluorescence microscopy. Annu Rev Biochem. 2009; 78:993-1016.
-
Huang B, Jones SA, Brandenburg B, Zhuang X. Whole-cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution. Nat Methods. 2008 Dec; 5(12):1047-52.
-
Bates M, Huang B, Zhuang X. Super-resolution microscopy by nanoscale localization of photo-switchable fluorescent probes. Curr Opin Chem Biol. 2008 Oct; 12(5):505-14.
-
Huang B, Wang W, Bates M, Zhuang X. Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy. Science. 2008 Feb 8; 319(5864):810-3.
-
Kim S, Huang B, Zare RN. Microfluidic separation and capture of analytes for single-molecule spectroscopy. Lab Chip. 2007 Dec; 7(12):1663-5.
-
Huang B, Kim S, Wu H, Zare RN. Use of a mixture of n-dodecyl-beta-D-maltoside and sodium dodecyl sulfate in poly(dimethylsiloxane) microchips to suppress adhesion and promote separation of proteins. Anal Chem. 2007 Dec 1; 79(23):9145-9.
-
Bates M, Huang B, Dempsey GT, Zhuang X. Multicolor super-resolution imaging with photo-switchable fluorescent probes. Science. 2007 Sep 21; 317(5845):1749-53.
-
Whorton MR, Bokoch MP, Rasmussen SG, Huang B, Zare RN, Kobilka B, Sunahara RK. A monomeric G protein-coupled receptor isolated in a high-density lipoprotein particle efficiently activates its G protein. Proc Natl Acad Sci U S A. 2007 May 1; 104(18):7682-7.
-
Huang B, Yu F, Zare RN. Surface plasmon resonance imaging using a high numerical aperture microscope objective. Anal Chem. 2007 Apr 1; 79(7):2979-83.
-
Huang B, Wu H, Bhaya D, Grossman A, Granier S, Kobilka BK, Zare RN. Counting low-copy number proteins in a single cell. Science. 2007 Jan 5; 315(5808):81-4.
-
Luo Y, Huang B, Wu H, Zare RN. Controlling electroosmotic flow in poly(dimethylsiloxane) separation channels by means of prepolymer additives. Anal Chem. 2006 Jul 1; 78(13):4588-92.
-
Wu H, Huang B, Zare RN. Generation of complex, static solution gradients in microfluidic channels. J Am Chem Soc. 2006 Apr 5; 128(13):4194-5.
-
Huang B, Wu H, Kim S, Kobilka BK, Zare RN. Phospholipid biotinylation of polydimethylsiloxane (PDMS) for protein immobilization. Lab Chip. 2006 Mar; 6(3):369-73.
-
Wu H, Huang B, Zare RN. Construction of microfluidic chips using polydimethylsiloxane for adhesive bonding. Lab Chip. 2005 Dec; 5(12):1393-8.
-
Huang B, Wu H, Kim S, Zare RN. Coating of poly(dimethylsiloxane) with n-dodecyl-beta-D-maltoside to minimize nonspecific protein adsorption. Lab Chip. 2005 Oct; 5(10):1005-7.
-
Perroud TD, Huang B, Zare RN. Effect of bin time on the photon counting histogram for one-photon excitation. Chemphyschem. 2005 May; 6(5):905-12.
-
Huang B, Perroud TD, Zare RN. Photon counting histogram: one-photon excitation. Chemphyschem. 2004 Oct 18; 5(10):1523-31.
-
Perroud TD, Huang B, Wallace MI, Zare RN. Photon counting histogram for one-photon excitation. Chemphyschem. 2003 Oct 17; 4(10):1121-3.
-
Ying L, Huang X, Huang B, Xie J, Zhao J, Zhao XS. Fluorescence emission and absorption spectra of single Anabaena sp. strain PCC7120 cells. Photochem Photobiol. 2002 Sep; 76(3):310-3.
-
Whelan RJ, Wohland T, Neumann L, Huang B, Kobilka BK, Zare RN. Analysis of biomolecular interactions using a miniaturized surface plasmon resonance sensor. Anal Chem. 2002 Sep 1; 74(17):4570-6.
|
Bo's Networks
Click the "See All" links for more information and interactive visualizations!
Related Concepts Derived automatically from this person's publications. _
Co-Authors People in Profiles who have published with this person. _
Related Authors People who share similar concepts with this person. _
|
