Todd Nystul, PhD
|School||UCSF School of Medicine|
|Address||513 Parnassus Ave|
San Francisco CA 94143
The follicular epithelium in the Drosophila ovary is an ideal model for the study of epithelial biology. It possesses many classical epithelial features, such as a columnar cell shape, apical/basal polarity, and canonical cell adhesion complexes, and yet is a relatively simple tissue and is highly tractable for molecular and cell biological analysis. Combined with the powerful genetic tools available in Drosophila, this allows us to address questions in epithelial stem cell and tissue biology with single-cell resolution in the natural, in vivo context.
Major Goals: Our laboratory uses the Drosophila ovary as a model for studying the fundamental properties of epithelial stem cells, their associated niche, and the connection between epithelial stem cells and cancer. We are interested in questions such as:
1. How is stem cell fate maintained within a dynamic epithelial tissue?
2. What is the nature of the epithelial stem cell niche?
3. What is the role of epithelial stem cells in normal reproductive physiology?
4. Do stem cell defects underlie epithelial cancers and what can studies of epithelial stem cells teach us about the earliest steps in cancer formation?
Identifying Stem Cells and their Niche
Although adult stem cells are believed to reside in distinct microenvironments, or niches, that function to regulate stem cell behavior, niches have been hard to study because of the difficulty of precisely identifying the stem cells in most tissues. However, we have developed a set of criteria that facilitates reliable identification of the epithelial follicle stem cells (FSCs) in the Drosophila ovary and we are now mapping their interactions with neighboring cells to better understand the nature of the FSC niche. Surprisingly, we have found that the FSC niche appears much more dynamic than the few previously characterized niches. We are using lineage analysis to follow FSC behavior, track the patterns of FSC daughter cell migration and differentiation, and investigate relevant gene function.
Epithelial Stem Cell Genetics
The wnt/wingless, hedgehog, BMP and Notch signaling pathways are all important for FSC function and early epithelial development but little is known about where in the process these signals exert their effects or how they are coordinated to produce a functional, healthy epithelium. We are investigating the function of key signaling components at specific steps in early follicle formation to map the contributions of these pathways to FSC function and follicle formation. In addition, we are interested in the role that misregulation of signaling in the ovarian epithelial cells plays in ovarian cancer. We are now investigating the interaction between the wnt pathway and other putative ovarian cancer genes in follicle cells as well as screening for markers that identify pre-tumorous cells to better understand the early steps leading to hyperplasia. Through collaboration with our colleagues in the Center for Reproductive Sciences, we will be able to test whether gene interactions and markers that we identify in the fly ovary will also be present in mammalian model systems and human tissues. To identify additional genes that are required for proper FSC function, we screened through a collection of over 600 lines bearing lethal mutations. We have identified several mutants with a follicle stem cell phenotype including ones that accelerate the rate of stem cell loss; confer a “hyper-competitive” stem cell replacement phenotype; and/or cause over-proliferation, perhaps modeling a precancerous state.
Epithelial Stem Cells and Oogenesis Oogenesis is well conserved from flies to mammals and studies of the Drosophila ovary have provided valuable insight into the process of female reproduction. Our work on the characterization of the follicle stem cells and their associated niche provides an opportunity to use the fly ovary as a model of follicle formation. By studying the lineage just downstream of the FSCs, we found that FSCs produce “pre-follicle cells” that are developmental intermediates between the FSCs and the polarized epithelium. Follicle formation begins when a newly formed germline cyst moves past the FSC niche and contacts these pre-follicle cells. We found that a Delta signal from the germline activates Notch in some pre-follicle cells, inducing them to migrate along the anterior fact of the cyst toward the opposite niche. Other pre-follicle cells that do not receive the signal instead migrate away from the niche toward the posterior, directly into the polarized epithelium. We are now studying how these and other cellular events lead to the formation of a new follicle during normal oogenesis.
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