Alex Hughes, PhD

TitlePostdoctoral Scholar
SchoolUCSF School of Pharmacy
DepartmentPharmaceutical Chemistry
Address600 16th Street
San Francisco CA 94158
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    University of California, San FranciscoPh.D.2008 Graduate Division (Bioengineering)

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    I develop and apply high-throughput methods to perturb and analyze biological systems.

    I believe that microscale analytical approaches have a tremendous role to play in the future of bioscience research, from diagnostic devices to basic biological inquiry at the single-cell level. As a postdoctoral researcher in the Gartner Lab at UCSF, I am working to determine the “design rules” that govern self-assembly of individual cells to form tissue-level structures by building and applying precision engineering methods for tissue assembly with single-cell resolution.

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    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. Todhunter ME, Jee NY, Hughes A, Coyle MC, Cerchiari A, Farlow J, Garbe JC, LaBarge MA, Desai TA, Gartner ZJ. Programmed synthesis of three-dimensional tissues. Nat Methods. 2015 Oct; 12(10):975-81. PMID: 26322836; PMCID: PMC4589502.
    2. Hughes A, Spelke DP, Xu Z, Kang CC, Schaffer DV, Herr AE. Single-cell western blotting. Nat Methods. 2014 Jul; 11(7):749-55. PMID: 24880876; PMCID: PMC4077215.
    3. Tentori AM, Hughes A, Herr AE. Microchamber integration unifies distinct separation modes for two-dimensional electrophoresis. Anal Chem. 2013 May 07; 85(9):4538-45. PMID: 23565932; PMCID: PMC3714212.
    4. Hughes A, Herr AE. Microfluidic Western blotting. Proc Natl Acad Sci U S A. 2012 Dec 26; 109(52):21450-5. PMID: 23223527; PMCID: PMC3535594.
    5. Hughes A, Tentori AM, Herr AE. Bistable isoelectric point photoswitching in green fluorescent proteins observed by dynamic immunoprobed isoelectric focusing. J Am Chem Soc. 2012 Oct 24; 134(42):17582-91. PMID: 23017083; PMCID: PMC3488114.
    6. Hughes A, Lin RK, Peehl DM, Herr AE. Microfluidic integration for automated targeted proteomic assays. Proc Natl Acad Sci U S A. 2012 Apr 17; 109(16):5972-7. PMID: 22474344; PMCID: PMC3341062.
    7. Chen X, Kapil MA, Hughes A, Herr AE. Single-microchannel, multistep assay reports protein size and immunoaffinity. Anal Chem. 2011 Sep 01; 83(17):6573-9. PMID: 21834519.
      View in: PubMed
    8. Hughes A, Herr AE. Quantitative enzyme activity determination with zeptomole sensitivity by microfluidic gradient-gel zymography. Anal Chem. 2010 May 01; 82(9):3803-11. PMID: 20353191.
      View in: PubMed
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