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Christine Guthrie, PhD

TitleProfessor Emeritus
InstitutionUniversity of California San Francisco
DepartmentBiochemistry and Biophysics
Address600 16th St
San Francisco CA 94158
Phone415-476-2321
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    Collapse Research 
    Collapse Research Activities and Funding
    Comprehensive Biology: Exploiting the Yeast Genome
    NIH P41RR011823Sep 30, 1996 - Jun 30, 2016
    Role: Co-Investigator
    Biosynthesis of RNAs
    NIH/NIGMS R01GM021119Feb 1, 1977 - Mar 31, 2016
    Role: Principal Investigator
    BIOSYNTHESIS OF RNAS
    NIH/NIGMS R37GM021119Feb 1, 1977 - Mar 31, 2000
    Role: Principal Investigator

    Collapse ORNG Applications 
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    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. MacRae AJ, Mayerle M, Hrabeta-Robinson E, Chalkley RJ, Guthrie C, Burlingame AL, Jurica M. Prp8 positioning of U5 snRNA is linked to 5' splice site recognition. RNA. 2018 Feb 27. PMID: 29487104.
      View in: PubMed
    2. Mayerle M, Raghavan M, Ledoux S, Price A, Stepankiw N, Hadjivassiliou H, Moehle EA, Mendoza SD, Pleiss JA, Guthrie C, Abelson J. Structural toggle in the RNaseH domain of Prp8 helps balance splicing fidelity and catalytic efficiency. Proc Natl Acad Sci U S A. 2017 05 02; 114(18):4739-4744. PMID: 28416677.
      View in: PubMed
    3. Nissen KE, Homer CM, Ryan CJ, Shales M, Krogan NJ, Patrick KL, Guthrie C. The histone variant H2A.Z promotes splicing of weak introns. Genes Dev. 2017 04 01; 31(7):688-701. PMID: 28446597.
      View in: PubMed
    4. Mayerle M, Guthrie C. Genetics and biochemistry remain essential in the structural era of the spliceosome. Methods. 2017 Aug 01; 125:3-9. PMID: 28132896.
      View in: PubMed
    5. Soucek S, Zeng Y, Bellur DL, Bergkessel M, Morris KJ, Deng Q, Duong D, Seyfried NT, Guthrie C, Staley JP, Fasken MB, Corbett AH. The Evolutionarily-conserved Polyadenosine RNA Binding Protein, Nab2, Cooperates with Splicing Machinery to Regulate the Fate of pre-mRNA. Mol Cell Biol. 2016 Aug 15. PMID: 27528618.
      View in: PubMed
    6. Ledoux S, Guthrie C. Retinitis Pigmentosa Mutations in Bad Response to Refrigeration 2 (Brr2) Impair ATPase and Helicase Activity. J Biol Chem. 2016 Jun 03; 291(23):11954-65. PMID: 27072132; PMCID: PMC4933248 [Available on 06/03/17].
    7. Mayerle M, Guthrie C. Prp8 retinitis pigmentosa mutants cause defects in the transition between the catalytic steps of splicing. RNA. 2016 May; 22(5):793-809. PMID: 26968627; PMCID: PMC4836653 [Available on 05/01/17].
    8. Mayerle M, Guthrie C. A new communication hub in the RNA world. Nat Struct Mol Biol. 2016 Mar; 23(3):189-90. PMID: 26931416.
      View in: PubMed
    9. Holmes RK, Tuck AC, Zhu C, Dunn-Davies HR, Kudla G, Clauder-Munster S, Granneman S, Steinmetz LM, Guthrie C, Tollervey D. Loss of the Yeast SR Protein Npl3 Alters Gene Expression Due to Transcription Readthrough. PLoS Genet. 2015 Dec; 11(12):e1005735. PMID: 26694144.
      View in: PubMed
    10. Lipp JJ, Marvin MC, Shokat KM, Guthrie C. SR protein kinases promote splicing of nonconsensus introns. Nat Struct Mol Biol. 2015 Aug; 22(8):611-7. PMID: 26167880.
      View in: PubMed
    11. Guthrie C. Present at the creation. RNA. 2015 Apr; 21(4):481. PMID: 25780103; PMCID: PMC4371245.
    12. Patrick KL, Ryan CJ, Xu J, Lipp JJ, Nissen KE, Roguev A, Shales M, Krogan NJ, Guthrie C. Genetic interaction mapping reveals a role for the SWI/SNF nucleosome remodeler in spliceosome activation in fission yeast. PLoS Genet. 2015 Mar; 11(3):e1005074. PMID: 25825871; PMCID: PMC4380400.
    13. Braberg H, Moehle EA, Shales M, Guthrie C, Krogan NJ. Genetic interaction analysis of point mutations enables interrogation of gene function at a residue-level resolution: exploring the applications of high-resolution genetic interaction mapping of point mutations. Bioessays. 2014 Jul; 36(7):706-13. PMID: 24842270; PMCID: PMC4289610.
    14. Moehle EA, Braberg H, Krogan NJ, Guthrie C. Adventures in time and space: splicing efficiency and RNA polymerase II elongation rate. RNA Biol. 2014; 11(4):313-9. PMID: 24717535; PMCID: PMC4075515.
    15. Hadjivassiliou H, Rosenberg OS, Guthrie C. The crystal structure of S. cerevisiae Sad1, a catalytically inactive deubiquitinase that is broadly required for pre-mRNA splicing. RNA. 2014 May; 20(5):656-69. PMID: 24681967; PMCID: PMC3988567.
    16. HĂ©rissant L, Moehle EA, Bertaccini D, Van Dorsselaer A, Schaeffer-Reiss C, Guthrie C, Dargemont C. H2B ubiquitylation modulates spliceosome assembly and function in budding yeast. Biol Cell. 2014 Apr; 106(4):126-38. PMID: 24476359; PMCID: PMC3989899.
    17. Krishnan R, Blanco MR, Kahlscheuer ML, Abelson J, Guthrie C, Walter NG. Biased Brownian ratcheting leads to pre-mRNA remodeling and capture prior to first-step splicing. Nat Struct Mol Biol. 2013 Dec; 20(12):1450-7. PMID: 24240612; PMCID: PMC3867266.
    18. Price AM, Görnemann J, Guthrie C, Brow DA. An unanticipated early function of DEAD-box ATPase Prp28 during commitment to splicing is modulated by U5 snRNP protein Prp8. RNA. 2014 Jan; 20(1):46-60. PMID: 24231520.
      View in: PubMed
    19. Braberg H, Jin H, Moehle EA, Chan YA, Wang S, Shales M, Benschop JJ, Morris JH, Qiu C, Hu F, Tang LK, Fraser JS, Holstege FC, Hieter P, Guthrie C, Kaplan CD, Krogan NJ. From structure to systems: high-resolution, quantitative genetic analysis of RNA polymerase II. Cell. 2013 Aug 15; 154(4):775-88. PMID: 23932120; PMCID: PMC3932829.
    20. Bergkessel M, Guthrie C, Abelson J. Yeast-gene replacement using PCR products. Methods Enzymol. 2013; 533:43-55. PMID: 24182917.
      View in: PubMed
    21. Bergkessel M, Guthrie C. Chemical transformation of yeast. Methods Enzymol. 2013; 529:311-20. PMID: 24011057.
      View in: PubMed
    22. Bergkessel M, Guthrie C. Colony PCR. Methods Enzymol. 2013; 529:299-309. PMID: 24011056.
      View in: PubMed
    23. Moehle EA, Ryan CJ, Krogan NJ, Kress TL, Guthrie C. The yeast SR-like protein Npl3 links chromatin modification to mRNA processing. PLoS Genet. 2012; 8(11):e1003101. PMID: 23209445; PMCID: PMC3510044.
    24. Ryan CJ, Roguev A, Patrick K, Xu J, Jahari H, Tong Z, Beltrao P, Shales M, Qu H, Collins SR, Kliegman JI, Jiang L, Kuo D, Tosti E, Kim HS, Edelmann W, Keogh MC, Greene D, Tang C, Cunningham P, Shokat KM, Cagney G, Svensson JP, Guthrie C, Espenshade PJ, Ideker T, Krogan NJ. Hierarchical modularity and the evolution of genetic interactomes across species. Mol Cell. 2012 Jun 08; 46(5):691-704. PMID: 22681890; PMCID: PMC3380636.
    25. Plocik AM, Guthrie C. Diverse forms of RPS9 splicing are part of an evolving autoregulatory circuit. PLoS Genet. 2012; 8(3):e1002620. PMID: 22479208; PMCID: PMC3315480.
    26. Bergkessel M, Whitworth GB, Guthrie C. Diverse environmental stresses elicit distinct responses at the level of pre-mRNA processing in yeast. RNA. 2011 Aug; 17(8):1461-78. PMID: 21697354; PMCID: PMC3153971.
    27. Ledoux S, Guthrie C. Regulation of the Dbp5 ATPase cycle in mRNP remodeling at the nuclear pore: a lively new paradigm for DEAD-box proteins. Genes Dev. 2011 Jun 01; 25(11):1109-14. PMID: 21632821; PMCID: PMC3110949.
    28. Huff JT, Plocik AM, Guthrie C, Yamamoto KR. Reciprocal intronic and exonic histone modification regions in humans. Nat Struct Mol Biol. 2010 Dec; 17(12):1495-9. PMID: 21057525; PMCID: PMC3057557.
    29. Mitrovich QM, Tuch BB, De La Vega FM, Guthrie C, Johnson AD. Evolution of yeast noncoding RNAs reveals an alternative mechanism for widespread intron loss. Science. 2010 Nov 05; 330(6005):838-41. PMID: 21051641; PMCID: PMC3496775.
    30. Abelson J, Blanco M, Ditzler MA, Fuller F, Aravamudhan P, Wood M, Villa T, Ryan DE, Pleiss JA, Maeder C, Guthrie C, Walter NG. Conformational dynamics of single pre-mRNA molecules during in vitro splicing. Nat Struct Mol Biol. 2010 Apr; 17(4):504-12. PMID: 20305654; PMCID: PMC2881217.
    31. Abelson J, Hadjivassiliou H, Guthrie C. Preparation of fluorescent pre-mRNA substrates for an smFRET study of pre-mRNA splicing in yeast. Methods Enzymol. 2010; 472:31-40. PMID: 20580958.
      View in: PubMed
    32. Guthrie C. From the ribosome to the spliceosome and back again. J Biol Chem. 2010 Jan 01; 285(1):1-12. PMID: 19880518; PMCID: PMC2804153.
    33. Zhang L, Xu T, Maeder C, Bud LO, Shanks J, Nix J, Guthrie C, Pleiss JA, Zhao R. Structural evidence for consecutive Hel308-like modules in the spliceosomal ATPase Brr2. Nat Struct Mol Biol. 2009 Jul; 16(7):731-9. PMID: 19525970; PMCID: PMC2743687.
    34. Wilmes GM, Guthrie C. Getting to the gate: crystallization of a Sac3(CID):Sus1:Cdc31 complex. Mol Cell. 2009 Mar 27; 33(6):671-2. PMID: 19328059.
      View in: PubMed
    35. Guthrie C, Schellenberg GD, Kraemer BC. SUT-2 potentiates tau-induced neurotoxicity in Caenorhabditis elegans. Hum Mol Genet. 2009 May 15; 18(10):1825-38. PMID: 19273536; PMCID: PMC2722225.
    36. Bergkessel M, Wilmes GM, Guthrie C. SnapShot: Formation of mRNPs. Cell. 2009 Feb 20; 136(4):794, 794.e1. PMID: 19239896.
      View in: PubMed
    37. Maeder C, Kutach AK, Guthrie C. ATP-dependent unwinding of U4/U6 snRNAs by the Brr2 helicase requires the C terminus of Prp8. Nat Struct Mol Biol. 2009 Jan; 16(1):42-8. PMID: 19098916; PMCID: PMC2707180.
    38. Wilmes GM, Bergkessel M, Bandyopadhyay S, Shales M, Braberg H, Cagney G, Collins SR, Whitworth GB, Kress TL, Weissman JS, Ideker T, Guthrie C, Krogan NJ. A genetic interaction map of RNA-processing factors reveals links between Sem1/Dss1-containing complexes and mRNA export and splicing. Mol Cell. 2008 Dec 05; 32(5):735-46. PMID: 19061648; PMCID: PMC2644724.
    39. Kress TL, Krogan NJ, Guthrie C. A single SR-like protein, Npl3, promotes pre-mRNA splicing in budding yeast. Mol Cell. 2008 Dec 05; 32(5):727-34. PMID: 19061647; PMCID: PMC2677966.
    40. Maeder C, Guthrie C. Modifications target spliceosome dynamics. Nat Struct Mol Biol. 2008 May; 15(5):426-8. PMID: 18461042.
      View in: PubMed
    41. Lund MK, Kress TL, Guthrie C. Autoregulation of Npl3, a yeast SR protein, requires a novel downstream region and serine phosphorylation. Mol Cell Biol. 2008 Jun; 28(11):3873-81. PMID: 18391019; PMCID: PMC2423289.
    42. Mitrovich QM, Guthrie C. Evolution of small nuclear RNAs in S. cerevisiae, C. albicans, and other hemiascomycetous yeasts. RNA. 2007 Dec; 13(12):2066-80. PMID: 17956975; PMCID: PMC2080600.
    43. Pleiss JA, Whitworth GB, Bergkessel M, Guthrie C. Rapid, transcript-specific changes in splicing in response to environmental stress. Mol Cell. 2007 Sep 21; 27(6):928-37. PMID: 17889666; PMCID: PMC2081968.
    44. Pleiss JA, Whitworth GB, Bergkessel M, Guthrie C. Transcript specificity in yeast pre-mRNA splicing revealed by mutations in core spliceosomal components. PLoS Biol. 2007 Apr; 5(4):e90. PMID: 17388687; PMCID: PMC1831718.
    45. Mitrovich QM, Tuch BB, Guthrie C, Johnson AD. Computational and experimental approaches double the number of known introns in the pathogenic yeast Candida albicans. Genome Res. 2007 Apr; 17(4):492-502. PMID: 17351132; PMCID: PMC1832096.
    46. Kim Guisbert KS, Li H, Guthrie C. Alternative 3' pre-mRNA processing in Saccharomyces cerevisiae is modulated by Nab4/Hrp1 in vivo. PLoS Biol. 2007 Jan; 5(1):e6. PMID: 17194212; PMCID: PMC1717019.
    47. Kress TL, Guthrie C. Molecular biology. Accurate RNA siting and splicing gets help from a DEK-hand. Science. 2006 Jun 30; 312(5782):1886-7. PMID: 16809518.
      View in: PubMed
    48. Preker PJ, Guthrie C. Autoregulation of the mRNA export factor Yra1p requires inefficient splicing of its pre-mRNA. RNA. 2006 Jun; 12(6):994-1006. PMID: 16618971; PMCID: PMC1464842.
    49. Brenner TJ, Guthrie C. Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domain. RNA. 2006 May; 12(5):862-71. PMID: 16540695; PMCID: PMC1440915.
    50. Bellare P, Kutach AK, Rines AK, Guthrie C, Sontheimer EJ. Ubiquitin binding by a variant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p. RNA. 2006 Feb; 12(2):292-302. PMID: 16428608; PMCID: PMC1370909.
    51. Lund MK, Guthrie C. The DEAD-box protein Dbp5p is required to dissociate Mex67p from exported mRNPs at the nuclear rim. Mol Cell. 2005 Nov 23; 20(4):645-51. PMID: 16307927.
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    52. Villa T, Guthrie C. The Isy1p component of the NineTeen complex interacts with the ATPase Prp16p to regulate the fidelity of pre-mRNA splicing. Genes Dev. 2005 Aug 15; 19(16):1894-904. PMID: 16103217; PMCID: PMC1186189.
    53. Brenner TJ, Guthrie C. Genetic analysis reveals a role for the C terminus of the Saccharomyces cerevisiae GTPase Snu114 during spliceosome activation. Genetics. 2005 Jul; 170(3):1063-80. PMID: 15911574; PMCID: PMC1451163.
    54. Kim Guisbert K, Duncan K, Li H, Guthrie C. Functional specificity of shuttling hnRNPs revealed by genome-wide analysis of their RNA binding profiles. RNA. 2005 Apr; 11(4):383-93. PMID: 15703440; PMCID: PMC1370728.
    55. Gilbert W, Guthrie C. The Glc7p nuclear phosphatase promotes mRNA export by facilitating association of Mex67p with mRNA. Mol Cell. 2004 Jan 30; 13(2):201-12. PMID: 14759366.
      View in: PubMed
    56. Inada M, Guthrie C. Identification of Lhp1p-associated RNAs by microarray analysis in Saccharomyces cerevisiae reveals association with coding and noncoding RNAs. Proc Natl Acad Sci U S A. 2004 Jan 13; 101(2):434-9. PMID: 14704279; PMCID: PMC327165.
    57. Rader SD, Guthrie C. A conserved Lsm-interaction motif in Prp24 required for efficient U4/U6 di-snRNP formation. RNA. 2002 Nov; 8(11):1378-92. PMID: 12458792; PMCID: PMC1370345.
    58. Preker PJ, Kim KS, Guthrie C. Expression of the essential mRNA export factor Yra1p is autoregulated by a splicing-dependent mechanism. RNA. 2002 Aug; 8(8):969-80. PMID: 12212852; PMCID: PMC1370319.
    59. Villa T, Pleiss JA, Guthrie C. Spliceosomal snRNAs: Mg(2+)-dependent chemistry at the catalytic core? Cell. 2002 Apr 19; 109(2):149-52. PMID: 12007401.
      View in: PubMed