Gilad Doitsh, PhD

Title(s)Full Specialist, Microbiology and Immunology
SchoolSchool of Medicine
Address600 16th Street
San Francisco CA 94158
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    UCSF2014  - 2015CFAR Early Career Investigator Award for Excellence in Basic Research.
    The Gladstone Institutes, UCSF2010  - 2011The Gladstone Institute of Virology and Immunology Award for Scientific Excellence

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    We are developing a new generation of RNA-based vaccines and adjuvants for mucosal delivery against viral infections that spread by the fecal-oral and respiratory routes. We hypothesize that enteroviral therapeutic interfering particles (eTIPs) are a powerful immunological adjuvant. eTIPs are generated from poliovirus positive-sense mRNA and carry a large deletion within the coding region for the capsid proteins. They cannot spread into the extracellular space, but they encode the RNA replication machinery that supports their intracellular amplification. This self-replicating RNA property and, particularly, the formation of cytosolic dsRNA intermediates greatly promote their recognition by PRRs, leading to production of interferon-stimulated genes within local target tissues.  
    eTIP-based mRNA formulations can potentially encode and drive the synthesis of other viral peptide immunogens, including Ebola, dengue, influenza and HIV-1. Of note, HIV-1 replicates preferentially in mucosal tissues, such as the intestinal mucosa, that are rich in memory CD4 T cells, and considered a major site of HIV reservoir. The few HIV vaccines that have been administered mucosally in human trials to date have not induced HIV-specific secretory IgA, but they were not specifically designed for mRNA-based mucosal application. Crossing the intestinal mucosal barrier could therefore open the door for development of a new therapeutic approach for controlling HIV-1 infections and purge its latent reservoir.

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    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help. to make corrections and additions.
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    Altmetrics Details PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. HIV-2 Depletes CD4 T Cells through Pyroptosis despite Vpx-Dependent Degradation of SAMHD1. J Virol. 2019 12 15; 93(24). Luo X, Herzig E, Doitsh G, Grimmett ZW, Muñoz-Arias I, Greene WC. PMID: 31578293.
      View in: PubMed   Mentions: 1     Fields:    Translation:HumansCells
    2. Distinct mechanisms regulate IL1B gene transcription in lymphoid CD4 T cells and monocytes. Cytokine. 2018 11; 111:373-381. Pulugulla SH, Packard TA, Galloway NLK, Grimmett ZW, Doitsh G, Adamik J, Galson DL, Greene WC, Auron PE. PMID: 30300855.
      View in: PubMed   Mentions: 5     Fields:    Translation:HumansCells
    3. Pyroptosis in HIV Infection: HIV Offense Meets a Fiery Host Defense. Encyclopedia of AIDS. 2018 Jan 1; 1774-1782. Gilad Doitsh. .
      View in: Publisher Site   Mentions:
    4. Dissecting How CD4 T Cells Are Lost During HIV Infection. . 2016 Mar 09; 19(3):280-91. Doitsh G, Greene WC. PMID: 26962940.
      View in: PubMed   Mentions:
    5. Blood-Derived CD4 T Cells Naturally Resist Pyroptosis during Abortive HIV-1 Infection. . 2015 Oct 14; 18(4):463-70. Muñoz-Arias I, Doitsh G, Yang Z, Sowinski S, Ruelas D, Greene WC. PMID: 26468749.
      View in: PubMed   Mentions:
    6. Cell-to-Cell Transmission of HIV-1 Is Required to Trigger Pyroptotic Death of Lymphoid-Tissue-Derived CD4 T Cells. Cell Rep. 2015 Sep 08; 12(10):1555-1563. Galloway NL, Doitsh G, Monroe KM, Yang Z, Muñoz-Arias I, Levy DN, Greene WC. PMID: 26321639.
      View in: PubMed   Mentions: 45     Fields:    Translation:HumansCells
    7. Pyroptosis in HIV Infection: HIV Offense Meets a Fiery Host Defense. Encyclopedia of AIDS. 2015 Jan 1; 1-9. Gilad Doitsh. .
      View in: Publisher Site   Mentions:
    8. F-103 Cell-to-cell transmission of HIV-1 is required to trigger pyroptotic death of CD4 T cells in lymphoid tissue. JAIDS Journal of Acquired Immune Deficiency Syndromes. 2014 Nov 1; 67(&NA;):63. Warner Greene, Nicole Galloway, Gilad Doitsh, Xin Geng, Zhiyuan Yang, Kathryn Monroe, Isa Munoz-Arias. .
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    9. Efficient delivery of lentiviral vectors into resting human CD4 T cells. Gene Ther. 2014 Apr; 21(4):444-9. Geng X, Doitsh G, Yang Z, Galloway NL, Greene WC. PMID: 24572792.
      View in: PubMed   Mentions: 10     Fields:    Translation:HumansAnimalsCells
    10. Cell death by pyroptosis drives CD4 T-cell depletion in HIV-1 infection. Nature. 2014 Jan 23; 505(7484):509-14. Doitsh G, Galloway NL, Geng X, Yang Z, Monroe KM, Zepeda O, Hunt PW, Hatano H, Sowinski S, Muñoz-Arias I, Greene WC. PMID: 24356306.
      View in: PubMed   Mentions: 348     Fields:    Translation:HumansCells
    11. IFI16 DNA sensor is required for death of lymphoid CD4 T cells abortively infected with HIV. Science. 2014 Jan 24; 343(6169):428-32. Monroe KM, Yang Z, Johnson JR, Geng X, Doitsh G, Krogan NJ, Greene WC. PMID: 24356113.
      View in: PubMed   Mentions: 184     Fields:    Translation:HumansCells
    12. 145 Pyroptosis and HIV-Induced CD4 T Cell Death. JAIDS Journal of Acquired Immune Deficiency Syndromes. 2012 Apr 1; 59(&NA;):61. Gilad Doitsh, Orlando Zepeda, Nicole Galloway, Isa Arias, Warner C. Greene. .
      View in: Publisher Site   Mentions:
    13. 113 Murder on the HIV Express: Insights into How CD4 T Cells Are Killed in Lymphoid Tissues. JAIDS Journal of Acquired Immune Deficiency Syndromes. 2011 Apr 1; 56:45. Gilad Doitsh, Marielle Cavrois, Kara Lassen, Orlando Zepeda, Zhiyuan Yang, Mario Santiago, Andrew M Hebbeler, Warner C Greene. .
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    14. Abortive HIV infection mediates CD4 T cell depletion and inflammation in human lymphoid tissue. Cell. 2010 Nov 24; 143(5):789-801. Doitsh G, Cavrois M, Lassen KG, Zepeda O, Yang Z, Santiago ML, Hebbeler AM, Greene WC. PMID: 21111238.
      View in: PubMed   Mentions: 187     Fields:    Translation:HumansCells
    15. Mechanisms of HIV-Infected vs. Uninfected T Cell Killing. Cell Death During HIV Infection. 2005 Nov 29; 207-228. Gilad Doitsh, Jason Kreisberg, Warner Greene. .
      View in: Publisher Site   Mentions:
    16. Enhancer I predominance in hepatitis B virus gene expression. Mol Cell Biol. 2004 Feb; 24(4):1799-808. Doitsh G, Shaul Y. PMID: 14749394.
      View in: PubMed   Mentions: 19     Fields:    Translation:Cells
    17. A long HBV transcript encoding pX is inefficiently exported from the nucleus. Virology. 2003 May 10; 309(2):339-49. Doitsh G, Shaul Y. PMID: 12758180.
      View in: PubMed   Mentions: 6     Fields:    Translation:HumansCells
    18. Hepatitis B virus pX interacts with HBXAP, a PHD finger protein to coactivate transcription. J Biol Chem. 2002 Mar 22; 277(12):9982-8. Shamay M, Barak O, Doitsh G, Ben-Dor I, Shaul Y. PMID: 11788598.
      View in: PubMed   Mentions: 23     Fields:    Translation:HumansAnimalsCells
    19. HBV transcription repression in response to genotoxic stress is p53-dependent and abrogated by pX. Oncogene. 1999 Dec 09; 18(52):7506-13. Doitsh G, Shaul Y. PMID: 10602509.
      View in: PubMed   Mentions: 8     Fields:    Translation:HumansCells
    20. Hepatitis B virus pX targets TFIIB in transcription coactivation. Mol Cell Biol. 1998 Mar; 18(3):1562-9. Haviv I, Shamay M, Doitsh G, Shaul Y. PMID: 9488473.
      View in: PubMed   Mentions: 43     Fields:    Translation:Cells
    21. p53 binds and represses the HBV enhancer: an adjacent enhancer element can reverse the transcription effect of p53. EMBO J. 1998 Jan 15; 17(2):544-53. Ori A, Zauberman A, Doitsh G, Paran N, Oren M, Shaul Y. PMID: 9430645.
      View in: PubMed   Mentions: 21     Fields:    Translation:HumansCells
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