Jeffrey Mugridge, PhD

Title(s)Associate Specialist, Pharmaceutical Chemistry
SchoolSchool of Pharmacy
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    Collapse Biography 
    Collapse Education and Training
    University of ChicagoBS (Honors)2006Chemistry
    University of California, BerkeleyPhD2010Chemistry
    Collapse Awards and Honors
    2013  - 2015NIH Ruth L. Kirschstein (F32) Postdoctoral Fellow
    2007  - 2010National Science Foundation Graduate Student Research Fellowship

    Collapse Overview 
    Collapse Overview
    I’m a multidisciplinary scientist with broad training in chemistry, biochemistry, and structural biology. My scientific work has used a diverse combination of structural, chemical, biochemical, and biophysical techniques to understand catalysis and molecular recognition in complex chemical and biological systems, ranging from synthetic supramolecular host-guest assemblies to biological multiprotein complexes.

    I'm currently studying the molecular mechanism and structure of the dynamic, multiprotein mRNA decapping complex, which removes the 5' cap found on all eukaryotic mRNA transcripts during a critical step in RNA decay. My work at UCSF has revealed the structural basis for substrate recognition and catalysis by the mRNA decapping enzyme Dcp2, and mechanisms by which protein cofactors engage the decapping complex to accelerate catalysis.

    Scientific Skill Set:
    • X-ray crystallography (protein & small molecule)
    • NMR spectroscopy (extensive 1D and 2D techniques with both proteins & small molecules)
    • Enzyme & chemical kinetics (in vitro biochemical kinetic assays, small molecule kinetic assays)
    • Fluorescence Anisotropy (protein-protein and protein-nucleic acid interactions in vitro)
    • Small-Angle X-ray Scattering (protein conformational changes and interactions)
    • Protein expression and purification (E. coli, affinity purification, AKTA FPLC, HPLC)
    • Chemical synthesis (organic and inorganic small molecules, including air-sensitive compounds)
    • Molecular modeling (molecular mechanics and DFT-level calculations on small molecules)

    Collapse Research 
    Collapse Research Activities and Funding
    Structural basis of mRNA decapping by Dcp2: conformational changes &co-activator
    NIH/NIGMS F32GM105313Feb 1, 2013 - Jan 31, 2015
    Role: Principal Investigator

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    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. Mugridge JS, Coller J, Gross JD. Publisher Correction: Structural and molecular mechanisms for the control of eukaryotic 5'-3' mRNA decay. Nat Struct Mol Biol. 2019 Feb; 26(2):147-148. PMID: 30575809.
      View in: PubMed
    2. Mugridge JS, Coller J, Gross JD. Structural and molecular mechanisms for the control of eukaryotic 5'-3' mRNA decay. Nat Struct Mol Biol. 2018 Dec; 25(12):1077-1085. PMID: 30518847.
      View in: PubMed
    3. Mugridge JS, Gross JD. Decapping enzymes STOP "cancer" ribosomes in their tracks. EMBO J. 2018 Dec 03; 37(23). PMID: 30455174.
      View in: PubMed
    4. Mugridge JS, Tibble RW, Ziemniak M, Jemielity J, Gross JD.Structure of the activated Edc1-Dcp1-Dcp2-Edc3 mRNA decapping complex with substrate analog poised for catalysis. Nature Communications. 2018; 1152(9).
    5. Mugridge JS, Tibble RW, Ziemniak M, Jemielity J, Gross JD. Structure of the activated Edc1-Dcp1-Dcp2-Edc3 mRNA decapping complex with substrate analog poised for catalysis. Nat Commun. 2018 03 20; 9(1):1152. PMID: 29559651.
      View in: PubMed
    6. Paquette DR, Mugridge JS, Weinberg DE, Gross JD. Application of a Schizosaccharomyces pombe Edc1-fused Dcp1-Dcp2 decapping enzyme for transcription start site mapping. RNA. 2018 02; 24(2):251-257. PMID: 29101277.
      View in: PubMed
    7. Mugridge JS, Ziemniak M, Jemielity J, Gross JD. Structural basis of mRNA-cap recognition by Dcp1-Dcp2. Nat Struct Mol Biol. 2016 Nov; 23(11):987-994. PMID: 27694842.
      View in: PubMed
    8. Ziemniak M, Mugridge JS, Kowalska J, Rhoads RE, Gross JD, Jemielity J. Two-headed tetraphosphate cap analogs are inhibitors of the Dcp1/2 RNA decapping complex. RNA. 2016 Apr; 22(4):518-29. PMID: 26826132; PMCID: PMC4793208 [Available on 04/01/17].
    9. Mugridge JS, Gross JD. Judge, jury, and executioner: DXO functions as a decapping enzyme and exoribonuclease in pre-mRNA quality control. Mol Cell. 2013 Apr 11; 50(1):2-4. PMID: 23582258; PMCID: PMC3665275.
    10. Mugridge JS, Zahl A, van Eldik R, Bergman RG, Raymond KN. Solvent and pressure effects on the motions of encapsulated guests: tuning the flexibility of a supramolecular host. J Am Chem Soc. 2013 Mar 20; 135(11):4299-306. PMID: 23391095.
      View in: PubMed
    11. Mugridge JS, Bergman RG, Raymond KN. Equilibrium isotope effects on noncovalent interactions in a supramolecular host-guest system. J Am Chem Soc. 2012 Feb 01; 134(4):2057-66. PMID: 22145944.
      View in: PubMed
    12. Mugridge JS, Bergman RG, Raymond KN. 1H NMR chemical shift calculations as a probe of supramolecular host-guest geometry. J Am Chem Soc. 2011 Jul 27; 133(29):11205-12. PMID: 21714506.
      View in: PubMed
    13. Mugridge JS, Szigethy G, Bergman RG, Raymond KN. Encapsulated guest-host dynamics: guest rotational barriers and tumbling as a probe of host interior cavity space. J Am Chem Soc. 2010 Nov 17; 132(45):16256-64. PMID: 20977233.
      View in: PubMed
    14. Mugridge JS, Fiedler D, Raymond KN.A ferrocene-based catecholamide ligand: the consequences of ligand swivel for directed supramolecular self-assembly. J Coord Chem. 2010; 63(14-16):2779-2789.
    15. Mugridge JS, Bergman RG, Raymond KN. Does size really matter? The steric isotope effect in a supramolecular host-guest exchange reaction. Angew Chem Int Ed Engl. 2010 May 10; 49(21):3635-7. PMID: 20394089.
      View in: PubMed
    16. Sun J, Shaner SE, Jones MK, O'Hanlon DC, Mugridge JS, Hopkins MD. Synthesis, structures, bonding, and redox chemistry of ditungsten butadiyne complexes with W[triple bond]C-C[triple bond]W backbones. Inorg Chem. 2010 Feb 15; 49(4):1687-98. PMID: 20095626.
      View in: PubMed
    17. Mugridge JS, Bergman RG, Raymond KN. High-precision measurement of isotope effects on noncovalent host-guest interactions. J Am Chem Soc. 2010 Feb 03; 132(4):1182-3. PMID: 20050653.
      View in: PubMed
    18. Sgarlata C, Mugridge JS, Pluth MD, Tiedemann BE, Zito V, Arena G, Raymond KN. External and internal guest binding of a highly charged supramolecular host in water: deconvoluting the very different thermodynamics. J Am Chem Soc. 2010 Jan 27; 132(3):1005-9. PMID: 20043697.
      View in: PubMed
    19. Pluth MD, Fiedler D, Mugridge JS, Bergman RG, Raymond KN. Molecular recognition and self-assembly special feature: Encapsulation and characterization of proton-bound amine homodimers in a water-soluble, self-assembled supramolecular host. Proc Natl Acad Sci U S A. 2009 Jun 30; 106(26):10438-43. PMID: 19181859; PMCID: PMC2705522.
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