Gary Cecchini, PhD

InstitutionUniversity of California San Francisco
DepartmentBiochemistry and Biophysics
Address4150 Clement Street
Phone415-221-4810 ext. 4416
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    Collapse Research 
    Collapse Research Activities and Funding
    BLR&D Research Career Scientist Award Application
    VA IK6BX004215Apr 1, 2018 - Mar 31, 2025
    Role: Principal Investigator
    VA I01BX001077Apr 1, 2011 - Mar 31, 2015
    Role: Principal Investigator
    Molecular &Cellular Bioenergetics Gordon Conference
    NIH/NIGMS R13GM071348May 18, 2004 - May 17, 2005
    Role: Principal Investigator
    Regulation of NADH: ubiquinone oxidoreductase (complex *
    NIH/FIC R03TW006041Aug 15, 2002 - Jun 30, 2008
    Role: Principal Investigator
    Structure/Function of Complex II Oxidoreductase
    NIH/NIGMS R01GM061606Jul 1, 2001 - Apr 30, 2018
    Role: Principal Investigator
    NIH/NHLBI P01HL016251Sep 1, 1978 - Jun 30, 2002
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. Maklashina E, Rajagukguk S, Iverson TM, Cecchini G. The unassembled flavoprotein subunits of human and bacterial complex II have impaired catalytic activity and generate only minor amounts of ROS. J Biol Chem. 2018 Apr 02. PMID: 29610278.
      View in: PubMed
    2. Sharma P, Maklashina E, Cecchini G, Iverson TM. Crystal structure of an assembly intermediate of respiratory Complex II. Nat Commun. 2018 Jan 18; 9(1):274. PMID: 29348404.
      View in: PubMed
    3. Starbird CA, Tomasiak TM, Singh PK, Yankovskaya V, Maklashina E, Eisenbach M, Cecchini G, Iverson TM. New crystal forms of the integral membrane Escherichia coli quinol:fumarate reductase suggest that ligands control domain movement. J Struct Biol. 2018 Apr; 202(1):100-104. PMID: 29158068.
      View in: PubMed
    4. Starbird CA, Maklashina E, Sharma P, Qualls-Histed S, Cecchini G, Iverson TM. Structural and biochemical analyses reveal insights into covalent flavinylation of the Escherichia coli Complex II homolog quinol:fumarate reductase. J Biol Chem. 2017 08 04; 292(31):12921-12933. PMID: 28615448.
      View in: PubMed
    5. Maklashina E, Rajagukguk S, Starbird CA, McDonald WH, Koganitsky A, Eisenbach M, Iverson TM, Cecchini G. Binding of the Covalent Flavin Assembly Factor to the Flavoprotein Subunit of Complex II. J Biol Chem. 2016 Feb 05; 291(6):2904-16. PMID: 26644464; PMCID: PMC4742753 [Available on 02/05/17].
    6. Cheng VW, Piragasam RS, Rothery RA, Maklashina E, Cecchini G, Weiner JH. Redox state of flavin adenine dinucleotide drives substrate binding and product release in Escherichia coli succinate dehydrogenase. Biochemistry. 2015 Feb 03; 54(4):1043-52. PMID: 25569225; PMCID: PMC4731035.
    7. Melin F, Noor MR, Pardieu E, Boulmedais F, Banhart F, Cecchini G, Soulimane T, Hellwig P. Investigating the thermostability of succinate: quinone oxidoreductase enzymes by direct electrochemistry at SWNTs-modified electrodes and FTIR spectroscopy. Chemphyschem. 2014 Nov 10; 15(16):3572-9. PMID: 25139263; PMCID: PMC4369915.
    8. Anderson RF, Shinde SS, Hille R, Rothery RA, Weiner JH, Rajagukguk S, Maklashina E, Cecchini G. Electron-transfer pathways in the heme and quinone-binding domain of complex II (succinate dehydrogenase). Biochemistry. 2014 Mar 18; 53(10):1637-46. PMID: 24559074; PMCID: PMC3985935.
    9. Singh PK, Sarwar M, Maklashina E, Kotlyar V, Rajagukguk S, Tomasiak TM, Cecchini G, Iverson TM. Plasticity of the quinone-binding site of the complex II homolog quinol:fumarate reductase. J Biol Chem. 2013 Aug 23; 288(34):24293-301. PMID: 23836905; PMCID: PMC3750132.
    10. Cheng VW, Tran QM, Boroumand N, Rothery RA, Maklashina E, Cecchini G, Weiner JH. A conserved lysine residue controls iron-sulfur cluster redox chemistry in Escherichia coli fumarate reductase. Biochim Biophys Acta. 2013 Oct; 1827(10):1141-7. PMID: 23711795; PMCID: PMC4354731.
    11. Cecchini G. Respiratory complex II: role in cellular physiology and disease. Biochim Biophys Acta. 2013 May; 1827(5):541-2. PMID: 23544420.
      View in: PubMed
    12. Maklashina E, Cecchini G, Dikanov SA. Defining a direction: electron transfer and catalysis in Escherichia coli complex II enzymes. Biochim Biophys Acta. 2013 May; 1827(5):668-78. PMID: 23396003; PMCID: PMC3615059.
    13. Iverson TM, Maklashina E, Cecchini G. Structural basis for malfunction in complex II. J Biol Chem. 2012 Oct 12; 287(42):35430-8. PMID: 22904323; PMCID: PMC3471735.
    14. Charruyer A, Strachan LR, Yue L, Toth AS, Cecchini G, Mancianti ML, Ghadially R. CD133 is a marker for long-term repopulating murine epidermal stem cells. J Invest Dermatol. 2012 Nov; 132(11):2522-33. PMID: 22763787; PMCID: PMC3997791.
    15. Lovett DH, Mahimkar R, Raffai RL, Cape L, Maklashina E, Cecchini G, Karliner JS. A novel intracellular isoform of matrix metalloproteinase-2 induced by oxidative stress activates innate immunity. PLoS One. 2012; 7(4):e34177. PMID: 22509276; PMCID: PMC3317925.
    16. Tran QM, Fong C, Rothery RA, Maklashina E, Cecchini G, Weiner JH. Out of plane distortions of the heme b of Escherichia coli succinate dehydrogenase. PLoS One. 2012; 7(2):e32641. PMID: 22393428; PMCID: PMC3290573.
    17. Zarbiv G, Li H, Wolf A, Cecchini G, Caplan SR, Sourjik V, Eisenbach M. Energy complexes are apparently associated with the switch-motor complex of bacterial flagella. J Mol Biol. 2012 Feb 17; 416(2):192-207. PMID: 22210351; PMCID: PMC4361073.
    18. Pyburn TM, Bensing BA, Xiong YQ, Melancon BJ, Tomasiak TM, Ward NJ, Yankovskaya V, Oliver KM, Cecchini G, Sulikowski GA, Tyska MJ, Sullam PM, Iverson TM. A structural model for binding of the serine-rich repeat adhesin GspB to host carbohydrate receptors. PLoS Pathog. 2011 Jul; 7(7):e1002112. PMID: 21765814; PMCID: PMC3131266.
    19. Ruprecht J, Iwata S, Rothery RA, Weiner JH, Maklashina E, Cecchini G. Perturbation of the quinone-binding site of complex II alters the electronic properties of the proximal [3Fe-4S] iron-sulfur cluster. J Biol Chem. 2011 Apr 08; 286(14):12756-65. PMID: 21310949; PMCID: PMC3069475.
    20. Kareyeva AV, Grivennikova VG, Cecchini G, Vinogradov AD. Molecular identification of the enzyme responsible for the mitochondrial NADH-supported ammonium-dependent hydrogen peroxide production. FEBS Lett. 2011 Jan 21; 585(2):385-9. PMID: 21168410; PMCID: PMC3022077.
    21. Tomasiak TM, Archuleta TL, Andréll J, Luna-Chávez C, Davis TA, Sarwar M, Ham AJ, McDonald WH, Yankovskaya V, Stern HA, Johnston JN, Maklashina E, Cecchini G, Iverson TM. Geometric restraint drives on- and off-pathway catalysis by the Escherichia coli menaquinol:fumarate reductase. J Biol Chem. 2011 Jan 28; 286(4):3047-56. PMID: 21098488; PMCID: PMC3024798.
    22. Pyburn TM, Yankovskaya V, Bensing BA, Cecchini G, Sullam PM, Iverson TM. Purification, crystallization and preliminary X-ray diffraction analysis of the carbohydrate-binding region of the Streptococcus gordonii adhesin GspB. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Nov 01; 66(Pt 11):1503-7. PMID: 21045307; PMCID: PMC3001660.
    23. Maklashina E, Cecchini G. The quinone-binding and catalytic site of complex II. Biochim Biophys Acta. 2010 Dec; 1797(12):1877-82. PMID: 20175986; PMCID: PMC2891400.
    24. Maklashina E, Rajagukguk S, McIntire WS, Cecchini G. Mutation of the heme axial ligand of Escherichia coli succinate-quinone reductase: implications for heme ligation in mitochondrial complex II from yeast. Biochim Biophys Acta. 2010 Jun-Jul; 1797(6-7):747-54. PMID: 20100456; PMCID: PMC2888824.
    25. Kotlyar AB, Randazzo A, Honbo N, Jin ZQ, Karliner JS, Cecchini G. Cardioprotective activity of a novel and potent competitive inhibitor of lactate dehydrogenase. FEBS Lett. 2010 Jan 04; 584(1):159-65. PMID: 19913538; PMCID: PMC2794998.
    26. Ruprecht J, Yankovskaya V, Maklashina E, Iwata S, Cecchini G. Structure of Escherichia coli succinate:quinone oxidoreductase with an occupied and empty quinone-binding site. J Biol Chem. 2009 Oct 23; 284(43):29836-46. PMID: 19710024; PMCID: PMC2785614.
    27. Gavrikova EV, Grivennikova VG, Borisov VB, Cecchini G, Vinogradov AD. Assembly of a chimeric respiratory chain from bovine heart submitochondrial particles and cytochrome bd terminal oxidase of Escherichia coli. FEBS Lett. 2009 Apr 17; 583(8):1287-91. PMID: 19303413; PMCID: PMC2703750.
    28. Grivennikova VG, Cecchini G, Vinogradov AD. Ammonium-dependent hydrogen peroxide production by mitochondria. FEBS Lett. 2008 Aug 06; 582(18):2719-24. PMID: 18619961.
      View in: PubMed
    29. Tomasiak TM, Maklashina E, Cecchini G, Iverson TM. A threonine on the active site loop controls transition state formation in Escherichia coli respiratory complex II. J Biol Chem. 2008 May 30; 283(22):15460-8. PMID: 18385138; PMCID: PMC2397489.
    30. Cohen-Ben-Lulu GN, Francis NR, Shimoni E, Noy D, Davidov Y, Prasad K, Sagi Y, Cecchini G, Johnstone RM, Eisenbach M. The bacterial flagellar switch complex is getting more complex. EMBO J. 2008 Apr 09; 27(7):1134-44. PMID: 18337747; PMCID: PMC2323253.
    31. Gostimskaya IS, Grivennikova VG, Cecchini G, Vinogradov AD. Reversible dissociation of flavin mononucleotide from the mammalian membrane-bound NADH: ubiquinone oxidoreductase (complex I). FEBS Lett. 2007 Dec 22; 581(30):5803-6. PMID: 18037377; PMCID: PMC2440658.
    32. Tran QM, Rothery RA, Maklashina E, Cecchini G, Weiner JH. Escherichia coli succinate dehydrogenase variant lacking the heme b. Proc Natl Acad Sci U S A. 2007 Nov 13; 104(46):18007-12. PMID: 17989224; PMCID: PMC2084287.
    33. Grivennikova VG, Kotlyar AB, Karliner JS, Cecchini G, Vinogradov AD. Redox-dependent change of nucleotide affinity to the active site of the mammalian complex I. Biochemistry. 2007 Sep 25; 46(38):10971-8. PMID: 17760425; PMCID: PMC2258335.
    34. Zhou HZ, Ma X, Gray MO, Zhu BQ, Nguyen AP, Baker AJ, Simonis U, Cecchini G, Lovett DH, Karliner JS. Transgenic MMP-2 expression induces latent cardiac mitochondrial dysfunction. Biochem Biophys Res Commun. 2007 Jun 22; 358(1):189-95. PMID: 17475219; PMCID: PMC3423089.
    35. Tomasiak TM, Cecchini G, Iverson TM. Succinate as Donor; Fumarate as Acceptor. EcoSal Plus. 2007 Apr; 2(2). PMID: 26443593.
      View in: PubMed
    36. Tran QM, Rothery RA, Maklashina E, Cecchini G, Weiner JH. The quinone binding site in Escherichia coli succinate dehydrogenase is required for electron transfer to the heme b. J Biol Chem. 2006 Oct 27; 281(43):32310-7. PMID: 16950775.
      View in: PubMed
    37. Maklashina E, Hellwig P, Rothery RA, Kotlyar V, Sher Y, Weiner JH, Cecchini G. Differences in protonation of ubiquinone and menaquinone in fumarate reductase from Escherichia coli. J Biol Chem. 2006 Sep 08; 281(36):26655-64. PMID: 16829675.
      View in: PubMed
    38. Zhu BQ, Simonis U, Cecchini G, Zhou HZ, Li L, Teerlink JR, Karliner JS. Comparison of pyrroloquinoline quinone and/or metoprolol on myocardial infarct size and mitochondrial damage in a rat model of ischemia/reperfusion injury. J Cardiovasc Pharmacol Ther. 2006 Jun; 11(2):119-28. PMID: 16891289.
      View in: PubMed
    39. Gostimskaya IS, Cecchini G, Vinogradov AD. Topography and chemical reactivity of the active-inactive transition-sensitive SH-group in the mitochondrial NADH:ubiquinone oxidoreductase (Complex I). Biochim Biophys Acta. 2006 Sep-Oct; 1757(9-10):1155-61. PMID: 16777054; PMCID: PMC2292829.
    40. Zhou HZ, Swanson RA, Simonis U, Ma X, Cecchini G, Gray MO. Poly(ADP-ribose) polymerase-1 hyperactivation and impairment of mitochondrial respiratory chain complex I function in reperfused mouse hearts. Am J Physiol Heart Circ Physiol. 2006 Aug; 291(2):H714-23. PMID: 16582021.
      View in: PubMed
    41. Maklashina E, Iverson TM, Sher Y, Kotlyar V, Andréll J, Mirza O, Hudson JM, Armstrong FA, Rothery RA, Weiner JH, Cecchini G. Fumarate reductase and succinate oxidase activity of Escherichia coli complex II homologs are perturbed differently by mutation of the flavin binding domain. J Biol Chem. 2006 Apr 21; 281(16):11357-65. PMID: 16484232.
      View in: PubMed
    42. Horsefield R, Yankovskaya V, Sexton G, Whittingham W, Shiomi K, Omura S, Byrne B, Cecchini G, Iwata S. Structural and computational analysis of the quinone-binding site of complex II (succinate-ubiquinone oxidoreductase): a mechanism of electron transfer and proton conduction during ubiquinone reduction. J Biol Chem. 2006 Mar 17; 281(11):7309-16. PMID: 16407191.
      View in: PubMed
    43. Kotlyar AB, Karliner JS, Cecchini G. A novel strong competitive inhibitor of complex I. FEBS Lett. 2005 Aug 29; 579(21):4861-6. PMID: 16107251.
      View in: PubMed
    44. Anderson RF, Hille R, Shinde SS, Cecchini G. Electron transfer within complex II. Succinate:ubiquinone oxidoreductase of Escherichia coli. J Biol Chem. 2005 Sep 30; 280(39):33331-7. PMID: 16085649.
      View in: PubMed
    45. Hudson JM, Heffron K, Kotlyar V, Sher Y, Maklashina E, Cecchini G, Armstrong FA. Electron transfer and catalytic control by the iron-sulfur clusters in a respiratory enzyme, E. coli fumarate reductase. J Am Chem Soc. 2005 May 18; 127(19):6977-89. PMID: 15884941.
      View in: PubMed
    46. Loskovich MV, Grivennikova VG, Cecchini G, Vinogradov AD. Inhibitory effect of palmitate on the mitochondrial NADH:ubiquinone oxidoreductase (complex I) as related to the active-de-active enzyme transition. Biochem J. 2005 May 01; 387(Pt 3):677-83. PMID: 15571492; PMCID: PMC1134997.
    47. Jin ZQ, Zhou HZ, Cecchini G, Gray MO, Karliner JS. MnSOD in mouse heart: acute responses to ischemic preconditioning and ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol. 2005 Jun; 288(6):H2986-94. PMID: 15681709.
      View in: PubMed
    48. Rothery RA, Seime AM, Spiers AM, Maklashina E, Schröder I, Gunsalus RP, Cecchini G, Weiner JH. Defining the Q-site of Escherichia coli fumarate reductase by site-directed mutagenesis, fluorescence quench titrations and EPR spectroscopy. FEBS J. 2005 Jan; 272(2):313-26. PMID: 15654871.
      View in: PubMed
    49. Kotlyar AB, Maklashina E, Cecchini G. Absence of NADH channeling in coupled reaction of mitochondrial malate dehydrogenase and complex I in alamethicin-permeabilized rat liver mitochondria. Biochem Biophys Res Commun. 2004 Jun 11; 318(4):987-91. PMID: 15147970.
      View in: PubMed
    50. Maklashina E, Kotlyar AB, Karliner JS, Cecchini G. Effect of oxygen on activation state of complex I and lack of oxaloacetate inhibition of complex II in Langendorff perfused rat heart. FEBS Lett. 2004 Jan 02; 556(1-3):64-8. PMID: 14706827.
      View in: PubMed
    51. Maklashina E, Kotlyar AB, Cecchini G. Active/de-active transition of respiratory complex I in bacteria, fungi, and animals. Biochim Biophys Acta. 2003 Sep 30; 1606(1-3):95-103. PMID: 14507430.
      View in: PubMed
    52. Cecchini G, Maklashina E, Yankovskaya V, Iverson TM, Iwata S. Variation in proton donor/acceptor pathways in succinate:quinone oxidoreductases. FEBS Lett. 2003 Jun 12; 545(1):31-8. PMID: 12788489.
      View in: PubMed
    53. Horsefield R, Yankovskaya V, Törnroth S, Luna-Chavez C, Stambouli E, Barber J, Byrne B, Cecchini G, Iwata S. Using rational screening and electron microscopy to optimize the crystallization of succinate:ubiquinone oxidoreductase from Escherichia coli. Acta Crystallogr D Biol Crystallogr. 2003 Mar; 59(Pt 3):600-2. PMID: 12595738.
      View in: PubMed
    54. Yankovskaya V, Horsefield R, Törnroth S, Luna-Chavez C, Miyoshi H, Léger C, Byrne B, Cecchini G, Iwata S. Architecture of succinate dehydrogenase and reactive oxygen species generation. Science. 2003 Jan 31; 299(5607):700-4. PMID: 12560550.
      View in: PubMed
    55. Cecchini G. Function and structure of complex II of the respiratory chain. Annu Rev Biochem. 2003; 72:77-109. PMID: 14527321.
      View in: PubMed
    56. Maklashina E, Sher Y, Zhou HZ, Gray MO, Karliner JS, Cecchini G. Effect of anoxia/reperfusion on the reversible active/de-active transition of NADH-ubiquinone oxidoreductase (complex I) in rat heart. Biochim Biophys Acta. 2002 Oct 03; 1556(1):6-12. PMID: 12351213.
      View in: PubMed
    57. Jeuken LJ, Jones AK, Chapman SK, Cecchini G, Armstrong FA. Electron-transfer mechanisms through biological redox chains in multicenter enzymes. J Am Chem Soc. 2002 May 22; 124(20):5702-13. PMID: 12010043.
      View in: PubMed
    58. Iverson TM, Luna-Chavez C, Croal LR, Cecchini G, Rees DC. Crystallographic studies of the Escherichia coli quinol-fumarate reductase with inhibitors bound to the quinol-binding site. J Biol Chem. 2002 May 03; 277(18):16124-30. PMID: 11850430.
      View in: PubMed
    59. Törnroth S, Yankovskaya V, Cecchini G, Iwata S. Purification, crystallisation and preliminary crystallographic studies of succinate:ubiquinone oxidoreductase from Escherichia coli. Biochim Biophys Acta. 2002 Jan 17; 1553(1-2):171-6. PMID: 11803025.
      View in: PubMed
    60. Cecchini G, Schröder I, Gunsalus RP, Maklashina E. Succinate dehydrogenase and fumarate reductase from Escherichia coli. Biochim Biophys Acta. 2002 Jan 17; 1553(1-2):140-57. PMID: 11803023.
      View in: PubMed