Robert Stroud, MA, PhD

InstitutionUniversity of California San Francisco
DepartmentBiochemistry and Biophysics
Address600 16th St
San Francisco CA 94158
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    Collapse Biography 
    Collapse Education and Training
    University of Cambridge, EnglandBA Nat Science1964Physics
    University of Cambridge, EnglandMA09/1968Physics and Chemistry
    University of London, EnglandMS09/1965Protein crystallography
    University of London, EnglandPh.D.09/1968Protein crystallography

    Collapse Research 
    Collapse Research Activities and Funding
    Mapping the conformational cycle of transmembrane transporters
    NIH/NIGMS P01GM111126Aug 1, 2015 - Jul 31, 2020
    Role: Principal Investigator
    Structural Basis of Vesicular Neurotransmitter Transport
    NIH/NINDS R01NS089713Aug 1, 2015 - Apr 30, 2019
    Role: Co-Principal Investigator
    4th NIH Roadmap Meeting on Membrane Protein Structures and Complexes
    NIH/NIGMS R13GM105348Sep 28, 2012 - Aug 31, 2013
    Role: Principal Investigator
    Center for Structure of Membrane Proteins
    NIH/NIGMS U54GM094625Sep 30, 2010 - Jun 30, 2015
    Role: Principal Investigator
    Anachem Lipidic Cubic Phase Crystallization Robot
    NIH/NCRR S10RR027234Feb 20, 2010 - Feb 19, 2012
    Role: Principal Investigator
    HARC Center: HIV Accessory and Regulatory Complexes
    NIH/NIGMS P50GM082250Aug 27, 2007 - Aug 31, 2022
    Role: Co-Investigator
    Specialized Center for the Protein Structure Initiative
    NIH/NIGMS U54GM074929Jul 4, 2005 - Jun 30, 2011
    Role: Principal Investigator
    2005 Mechanisms of Membrane Transport Gordon Conference
    NIH/NIDDK R13DK071453Apr 1, 2005 - Mar 31, 2006
    Role: Principal Investigator
    Membrane Protein Expression Center
    NIH/NIGMS P50GM073210Sep 24, 2004 - Aug 31, 2014
    Role: Principal Investigator
    Signal Sequence Recognition and Protein Targeting
    NIH/NIGMS R01GM060641Jan 1, 2000 - Jun 30, 2009
    Role: Principal Investigator
    Structure Biology and Targeted Drug Design for AIDS
    NIH/NIGMS P01GM056531Sep 1, 1997 - Aug 31, 2007
    Role: Co-Investigator
    RNA Modification Enzymes: Methyltransferase/PSI-synthase
    NIH/NIGMS R01GM051232May 1, 1996 - May 31, 2013
    Role: Principal Investigator
    NIH/NCI R01CA063081Jan 1, 1995 - Jun 30, 2006
    Role: Principal Investigator
    NIH/NCRR S10RR006474Aug 12, 1991 - Sep 30, 1993
    Role: Principal Investigator
    NIH/NIGMS P01GM039552Sep 1, 1987 - Aug 31, 1997
    Role: Co-Investigator
    NIH/NCRR S10RR002705Dec 20, 1985 - Dec 19, 1986
    Role: Principal Investigator
    NIH/NIADDK R13AM036248Jul 1, 1985 - Jun 30, 1986
    Role: Principal Investigator
    3 D Electron Microscopy of Macromolecules
    NIH P41RR002250Mar 1, 1985 - Dec 31, 2014
    Role: Co-Investigator
    NIH/NIGMS R01GM032079Jun 1, 1983 - May 31, 1993
    Role: Principal Investigator
    Bio-Organic Biomedical Mass Spectrometry Resource
    NIH P41RR001614Mar 1, 1982 - May 31, 2015
    Role: Co-Investigator
    NIH P01GM027057Jun 1, 1980 - Jun 30, 1991
    Role: Co-Investigator
    A Synchroton Radiation Structural Biology Resource
    NIH P41RR001209Mar 1, 1980 - Feb 28, 2015
    Role: Co-Investigator
    Molecular Basis for Transmembrane Conduction & Signaling
    NIH/NIGMS R01GM024485Apr 1, 1979 - Dec 31, 2020
    Role: Principal Investigator
    Molecular Basis for Transmembrane Conduction & Signaling
    NIH/NIGMS R37GM024485Apr 1, 1979 - Dec 31, 2016
    Role: Principal Investigator
    NIH/NCI R01CA014394Sep 1, 1978 - Feb 28, 2002
    Role: Principal Investigator
    Resource for Biocomputing, Visualization, and Informatics
    NIH/NCRR P41RR001081Jun 1, 1976 - Sep 14, 2012
    Role: Co-Investigator

    Collapse ORNG Applications 
    Collapse Global Health
    Collapse Websites

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. Kintzer AF, Green EM, Dominik PK, Bridges M, Armache JP, Deneka D, Kim SS, Hubbell W, Kossiakoff AA, Cheng Y, Stroud RM. Structural basis for activation of voltage sensor domains in an ion channel TPC1. Proc Natl Acad Sci U S A. 2018 Sep 06. PMID: 30190435.
      View in: PubMed
    2. Chen SH, Jang GM, Hüttenhain R, Gordon DE, Du D, Newton BW, Johnson JR, Hiatt J, Hultquist JF, Johnson TL, Liu YL, Burton LA, Ye J, Reichermeier KM, Stroud RM, Marson A, Debnath J, Gross JD, Krogan NJ. CRL4AMBRA1 targets Elongin C for ubiquitination and degradation to modulate CRL5 signaling. EMBO J. 2018 Aug 30. PMID: 30166453.
      View in: PubMed
    3. Kumar H, Finer-Moore JS, Jiang X, Smirnova I, Kasho V, Pardon E, Steyaert J, Kaback HR, Stroud RM. Crystal Structure of a ligand-bound LacY-Nanobody Complex. Proc Natl Acad Sci U S A. 2018 Aug 14. PMID: 30108145.
      View in: PubMed
    4. Finer-Moore JS, Lee TT, Stroud RM. A Single Mutation Traps a Half-Sites Reactive Enzyme in Midstream, Explaining Asymmetry in Hydride Transfer. Biochemistry. 2018 May 15; 57(19):2786-2795. PMID: 29717875.
      View in: PubMed
    5. Kintzer AF, Stroud RM. On the structure and mechanism of two-pore channels. FEBS J. 2018 Jan; 285(2):233-243. PMID: 28656706.
      View in: PubMed
    6. Nöll A, Thomas C, Herbring V, Zollmann T, Barth K, Mehdipour AR, Tomasiak TM, Brüchert S, Joseph B, Abele R, Oliéric V, Wang M, Diederichs K, Hummer G, Stroud RM, Pos KM, Tampé R. Crystal structure and mechanistic basis of a functional homolog of the antigen transporter TAP. Proc Natl Acad Sci U S A. 2017 01 24; 114(4):E438-E447. PMID: 28069938.
      View in: PubMed
    7. Galilee M, Britan-Rosich E, Griner SL, Uysal S, Baumgärtel V, Lamb DC, Kossiakoff AA, Kotler M, Stroud RM, Marx A, Alian A. The Preserved HTH-Docking Cleft of HIV-1 Integrase Is Functionally Critical. Structure. 2016 11 01; 24(11):1936-1946. PMID: 27692964.
      View in: PubMed
    8. Thurtle-Schmidt BH, Stroud RM. Structure of Bor1 supports an elevator transport mechanism for SLC4 anion exchangers. Proc Natl Acad Sci U S A. 2016 09 20; 113(38):10542-6. PMID: 27601653.
      View in: PubMed
    9. Kapoor K, Finer-Moore JS, Pedersen BP, Caboni L, Waight A, Hillig RC, Bringmann P, Heisler I, Müller T, Siebeneicher H, Stroud RM. Mechanism of inhibition of human glucose transporter GLUT1 is conserved between cytochalasin B and phenylalanine amides. Proc Natl Acad Sci U S A. 2016 Apr 26; 113(17):4711-6. PMID: 27078104; PMCID: PMC4855560 [Available on 10/26/16].
    10. Kintzer AF, Stroud RM. Structure, inhibition and regulation of two-pore channel TPC1 from Arabidopsis thaliana. Nature. 2016 Mar 10; 531(7593):258-62. PMID: 26961658; PMCID: PMC4863712.
    11. Chaudhary S, Saha S, Thamminana S, Stroud RM. Small-Scale Screening to Large-Scale Over-Expression of Human Membrane Proteins for Structural Studies. Methods Mol Biol. 2016; 1432:203-21. PMID: 27485338.
      View in: PubMed
    12. Boswell-Casteel RC, Johnson JM, Stroud RM, Hays FA. Integral Membrane Protein Expression in Saccharomyces cerevisiae. Methods Mol Biol. 2016; 1432:163-86. PMID: 27485336.
      View in: PubMed
    13. Finer-Moore J, Czudnochowski N, O'Connell JD, Wang AL, Stroud RM. Crystal Structure of the Human tRNA m(1)A58 Methyltransferase-tRNA(3)(Lys) Complex: Refolding of Substrate tRNA Allows Access to the Methylation Target. J Mol Biol. 2015 Dec 04; 427(24):3862-76. PMID: 26470919; PMCID: PMC4663122 [Available on 12/04/16].
    14. Johri AK, Oelmüller R, Dua M, Yadav V, Kumar M, Tuteja N, Varma A, Bonfante P, Persson BL, Stroud RM. Fungal association and utilization of phosphate by plants: success, limitations, and future prospects. Front Microbiol. 2015; 6:984. PMID: 26528243; PMCID: PMC4608361.
    15. Kumar H, Finer-Moore JS, Kaback HR, Stroud RM. Structure of LacY with an a-substituted galactoside: Connecting the binding site to the protonation site. Proc Natl Acad Sci U S A. 2015 Jul 21; 112(29):9004-9. PMID: 26157133; PMCID: PMC4517220.
    16. Rosenberg OS, Dovala D, Li X, Connolly L, Bendebury A, Finer-Moore J, Holton J, Cheng Y, Stroud RM, Cox JS. Substrates Control Multimerization and Activation of the Multi-Domain ATPase Motor of Type VII Secretion. Cell. 2015 Apr 23; 161(3):501-512. PMID: 25865481; PMCID: PMC4409929.
    17. Salo-Ahen OM, Tochowicz A, Pozzi C, Cardinale D, Ferrari S, Boum Y, Mangani S, Stroud RM, Saxena P, Myllykallio H, Costi MP, Ponterini G, Wade RC. Hotspots in an obligate homodimeric anticancer target. Structural and functional effects of interfacial mutations in human thymidylate synthase. J Med Chem. 2015 Apr 23; 58(8):3572-81. PMID: 25798950; PMCID: PMC4634673.
    18. Tochowicz A, Santucci M, Saxena P, Guaitoli G, Trande M, Finer-Moore J, Stroud RM, Costi MP. Alanine mutants of the interface residues of human thymidylate synthase decode key features of the binding mode of allosteric anticancer peptides. J Med Chem. 2015 Jan 22; 58(2):1012-8. PMID: 25427005.
      View in: PubMed
    19. Kim J, Wu S, Tomasiak TM, Mergel C, Winter MB, Stiller SB, Robles-Colmanares Y, Stroud RM, Tampé R, Craik CS, Cheng Y. Subnanometre-resolution electron cryomicroscopy structure of a heterodimeric ABC exporter. Nature. 2015 Jan 15; 517(7534):396-400. PMID: 25363761; PMCID: PMC4372080.
    20. Tomasiak TM, Pedersen BP, Chaudhary S, Rodriguez A, Colmanares YR, Roe-Zurz Z, Thamminana S, Tessema M, Stroud RM. General qPCR and Plate Reader Methods for Rapid Optimization of Membrane Protein Purification and Crystallization Using Thermostability Assays. Curr Protoc Protein Sci. 2014 Aug 01; 77:29.11.1-14. PMID: 25081745; PMCID: PMC4672949.
    21. Miercke LJ, Robbins RA, Stroud RM. Tetra detector analysis of membrane proteins. Curr Protoc Protein Sci. 2014 Aug 01; 77:29.10.1-30. PMID: 25081744; PMCID: PMC4178957.
    22. Lohse MB, Rosenberg OS, Cox JS, Stroud RM, Finer-Moore JS, Johnson AD. Structure of a new DNA-binding domain which regulates pathogenesis in a wide variety of fungi. Proc Natl Acad Sci U S A. 2014 Jul 22; 111(29):10404-10. PMID: 24994900; PMCID: PMC4115540.
    23. Monk BC, Tomasiak TM, Keniya MV, Huschmann FU, Tyndall JD, O'Connell JD, Cannon RD, McDonald JG, Rodriguez A, Finer-Moore JS, Stroud RM. Architecture of a single membrane spanning cytochrome P450 suggests constraints that orient the catalytic domain relative to a bilayer. Proc Natl Acad Sci U S A. 2014 Mar 11; 111(10):3865-70. PMID: 24613931; PMCID: PMC3956205.
    24. Kumar H, Kasho V, Smirnova I, Finer-Moore JS, Kaback HR, Stroud RM. Structure of sugar-bound LacY. Proc Natl Acad Sci U S A. 2014 Feb 04; 111(5):1784-8. PMID: 24453216; PMCID: PMC3918835.
    25. Czudnochowski N, Ashley GW, Santi DV, Alian A, Finer-Moore J, Stroud RM. The mechanism of pseudouridine synthases from a covalent complex with RNA, and alternate specificity for U2605 versus U2604 between close homologs. Nucleic Acids Res. 2014 Feb; 42(3):2037-48. PMID: 24214967; PMCID: PMC3919597.
    26. Pak JE, Ekendé EN, Kifle EG, O'Connell JD, De Angelis F, Tessema MB, Derfoufi KM, Robles-Colmenares Y, Robbins RA, Goormaghtigh E, Vandenbussche G, Stroud RM. Structures of intermediate transport states of ZneA, a Zn(II)/proton antiporter. Proc Natl Acad Sci U S A. 2013 Nov 12; 110(46):18484-9. PMID: 24173033; PMCID: PMC3832018.
    27. Tochowicz A, Dalziel S, Eidam O, O'Connell JD, Griner S, Finer-Moore JS, Stroud RM. Development and binding mode assessment of N-[4-[2-propyn-1-yl[(6S)-4,6,7,8-tetrahydro-2-(hydroxymethyl)-4-oxo-3H-cyclopenta[g]quinazolin-6-yl]amino]benzoyl]-l-?-glutamyl-D-glutamic acid (BGC 945), a novel thymidylate synthase inhibitor that targets tumor cells. J Med Chem. 2013 Jul 11; 56(13):5446-55. PMID: 23710599; PMCID: PMC3880649.
    28. Czudnochowski N, Wang AL, Finer-Moore J, Stroud RM. In human pseudouridine synthase 1 (hPus1), a C-terminal helical insert blocks tRNA from binding in the same orientation as in the Pus1 bacterial homologue TruA, consistent with their different target selectivities. J Mol Biol. 2013 Oct 23; 425(20):3875-87. PMID: 23707380; PMCID: PMC3900414.
    29. Waight AB, Pedersen BP, Schlessinger A, Bonomi M, Chau BH, Roe-Zurz Z, Risenmay AJ, Sali A, Stroud RM. Structural basis for alternating access of a eukaryotic calcium/proton exchanger. Nature. 2013 Jul 04; 499(7456):107-10. PMID: 23685453; PMCID: PMC3702627.
    30. Wang Z, Sapienza PJ, Abeysinghe T, Luzum C, Lee AL, Finer-Moore JS, Stroud RM, Kohen A. Mg2+ binds to the surface of thymidylate synthase and affects hydride transfer at the interior active site. J Am Chem Soc. 2013 May 22; 135(20):7583-92. PMID: 23611499; PMCID: PMC3674108.
    31. Pedersen BP, Kumar H, Waight AB, Risenmay AJ, Roe-Zurz Z, Chau BH, Schlessinger A, Bonomi M, Harries W, Sali A, Johri AK, Stroud RM. Crystal structure of a eukaryotic phosphate transporter. Nature. 2013 Apr 25; 496(7446):533-6. PMID: 23542591; PMCID: PMC3678552.
    32. Pieper U, Schlessinger A, Kloppmann E, Chang GA, Chou JJ, Dumont ME, Fox BG, Fromme P, Hendrickson WA, Malkowski MG, Rees DC, Stokes DL, Stowell MH, Wiener MC, Rost B, Stroud RM, Stevens RC, Sali A. Coordinating the impact of structural genomics on the human a-helical transmembrane proteome. Nat Struct Mol Biol. 2013 Feb; 20(2):135-8. PMID: 23381628; PMCID: PMC3645303.
    33. Carosati E, Tochowicz A, Marverti G, Guaitoli G, Benedetti P, Ferrari S, Stroud RM, Finer-Moore J, Luciani R, Farina D, Cruciani G, Costi MP. Inhibitor of ovarian cancer cells growth by virtual screening: a new thiazole derivative targeting human thymidylate synthase. J Med Chem. 2012 Nov 26; 55(22):10272-6. PMID: 23075414.
      View in: PubMed
    34. Wang Z, Abeysinghe T, Finer-Moore JS, Stroud RM, Kohen A. A remote mutation affects the hydride transfer by disrupting concerted protein motions in thymidylate synthase. J Am Chem Soc. 2012 Oct 24; 134(42):17722-30. PMID: 23034004; PMCID: PMC3490427.
    35. Metzger LE, Lee JK, Finer-Moore JS, Raetz CR, Stroud RM. LpxI structures reveal how a lipid A precursor is synthesized. Nat Struct Mol Biol. 2012 Nov; 19(11):1132-8. PMID: 23042606; PMCID: PMC3562136.
    36. Pozzi C, Ferrari S, Cortesi D, Luciani R, Stroud RM, Catalano A, Costi MP, Mangani S. The structure of Enterococcus faecalis thymidylate synthase provides clues about folate bacterial metabolism. Acta Crystallogr D Biol Crystallogr. 2012 Sep; 68(Pt 9):1232-41. PMID: 22948925.
      View in: PubMed
    37. Varrin-Doyer M, Spencer CM, Schulze-Topphoff U, Nelson PA, Stroud RM, Cree BA, Zamvil SS. Aquaporin 4-specific T cells in neuromyelitis optica exhibit a Th17 bias and recognize Clostridium ABC transporter. Ann Neurol. 2012 Jul; 72(1):53-64. PMID: 22807325; PMCID: PMC3405197.
    38. Egea PF, Muller-Steffner H, Kuhn I, Cakir-Kiefer C, Oppenheimer NJ, Stroud RM, Kellenberger E, Schuber F. Insights into the mechanism of bovine CD38/NAD+glycohydrolase from the X-ray structures of its Michaelis complex and covalently-trapped intermediates. PLoS One. 2012; 7(4):e34918. PMID: 22529956.
      View in: PubMed
    39. Wu S, Avila-Sakar A, Kim J, Booth DS, Greenberg CH, Rossi A, Liao M, Li X, Alian A, Griner SL, Juge N, Yu Y, Mergel CM, Chaparro-Riggers J, Strop P, Tampé R, Edwards RH, Stroud RM, Craik CS, Cheng Y. Fabs enable single particle cryoEM studies of small proteins. Structure. 2012 Apr 04; 20(4):582-92. PMID: 22483106; PMCID: PMC3322386.
    40. Chaudhary S, Pak JE, Gruswitz F, Sharma V, Stroud RM. Overexpressing human membrane proteins in stably transfected and clonal human embryonic kidney 293S cells. Nat Protoc. 2012 Feb 09; 7(3):453-66. PMID: 22322218; PMCID: PMC3613139.
    41. Kim J, Stroud RM, Craik CS. Rapid identification of recombinant Fabs that bind to membrane proteins. Methods. 2011 Dec; 55(4):303-9. PMID: 21958987; PMCID: PMC3264787.
    42. Chaudhary S, Pak JE, Pedersen BP, Bang LJ, Zhang LB, Ngaw SM, Green RG, Sharma V, Stroud RM. Efficient expression screening of human membrane proteins in transiently transfected Human Embryonic Kidney 293S cells. Methods. 2011 Dec; 55(4):273-80. PMID: 21925269; PMCID: PMC3600976.
    43. Stroud RM, Schertler GF. Membranes. Curr Opin Struct Biol. 2011 Aug; 21(4):495-6. PMID: 21875531.
      View in: PubMed
    44. Rosenberg OS, Dovey C, Tempesta M, Robbins RA, Finer-Moore JS, Stroud RM, Cox JS. EspR, a key regulator of Mycobacterium tuberculosis virulence, adopts a unique dimeric structure among helix-turn-helix proteins. Proc Natl Acad Sci U S A. 2011 Aug 16; 108(33):13450-5. PMID: 21795602; PMCID: PMC3158157.
    45. Korennykh AV, Korostelev AA, Egea PF, Finer-Moore J, Stroud RM, Zhang C, Shokat KM, Walter P. Structural and functional basis for RNA cleavage by Ire1. BMC Biol. 2011 Jul 06; 9:47. PMID: 21729333; PMCID: PMC3149027.
    46. Korennykh AV, Egea PF, Korostelev AA, Finer-Moore J, Stroud RM, Zhang C, Shokat KM, Walter P. Cofactor-mediated conformational control in the bifunctional kinase/RNase Ire1. BMC Biol. 2011 Jul 06; 9:48. PMID: 21729334; PMCID: PMC3158555.
    47. Stroud RM. New tools in membrane protein determination. F1000 Biol Rep. 2011; 3:8. PMID: 21655333; PMCID: PMC3100781.
    48. Nelson PA, Khodadoust M, Prodhomme T, Spencer C, Patarroyo JC, Varrin-Doyer M, Ho JD, Stroud RM, Zamvil SS. Immunodominant T cell determinants of aquaporin-4, the autoantigen associated with neuromyelitis optica. PLoS One. 2010 Nov 30; 5(11):e15050. PMID: 21151500; PMCID: PMC2994828.
    49. Savage DF, O'Connell JD, Miercke LJ, Finer-Moore J, Stroud RM. Structural context shapes the aquaporin selectivity filter. Proc Natl Acad Sci U S A. 2010 Oct 05; 107(40):17164-9. PMID: 20855585; PMCID: PMC2951435.
    50. Egea PF, Stroud RM. Lateral opening of a translocon upon entry of protein suggests the mechanism of insertion into membranes. Proc Natl Acad Sci U S A. 2010 Oct 05; 107(40):17182-7. PMID: 20855604; PMCID: PMC2951439.
    51. Lee JK, Stroud RM. Unlocking the eukaryotic membrane protein structural proteome. Curr Opin Struct Biol. 2010 Aug; 20(4):464-70. PMID: 20739007; PMCID: PMC3530418.
    52. De Angelis F, Lee JK, O'Connell JD, Miercke LJ, Verschueren KH, Srinivasan V, Bauvois C, Govaerts C, Robbins RA, Ruysschaert JM, Stroud RM, Vandenbussche G. Metal-induced conformational changes in ZneB suggest an active role of membrane fusion proteins in efflux resistance systems. Proc Natl Acad Sci U S A. 2010 Jun 15; 107(24):11038-43. PMID: 20534468; PMCID: PMC2890744.
    53. Gruswitz F, Chaudhary S, Ho JD, Schlessinger A, Pezeshki B, Ho CM, Sali A, Westhoff CM, Stroud RM. Function of human Rh based on structure of RhCG at 2.1 A. Proc Natl Acad Sci U S A. 2010 May 25; 107(21):9638-43. PMID: 20457942; PMCID: PMC2906887.
    54. Schlessinger A, Matsson P, Shima JE, Pieper U, Yee SW, Kelly L, Apeltsin L, Stroud RM, Ferrin TE, Giacomini KM, Sali A. Comparison of human solute carriers. Protein Sci. 2010 Mar; 19(3):412-28. PMID: 20052679; PMCID: PMC2866268.
    55. Hays FA, Roe-Zurz Z, Stroud RM. Overexpression and purification of integral membrane proteins in yeast. Methods Enzymol. 2010; 470:695-707. PMID: 20946832.
      View in: PubMed
    56. Kelly L, Pieper U, Eswar N, Hays FA, Li M, Roe-Zurz Z, Kroetz DL, Giacomini KM, Stroud RM, Sali A. A survey of integral alpha-helical membrane proteins. J Struct Funct Genomics. 2009 Dec; 10(4):269-80. PMID: 19760129.
      View in: PubMed
    57. Alian A, Griner SL, Chiang V, Tsiang M, Jones G, Birkus G, Geleziunas R, Leavitt AD, Stroud RM. Catalytically-active complex of HIV-1 integrase with a viral DNA substrate binds anti-integrase drugs. Proc Natl Acad Sci U S A. 2009 May 19; 106(20):8192-7. PMID: 19416821; PMCID: PMC2688900.
    58. Ho JD, Yeh R, Sandstrom A, Chorny I, Harries WE, Robbins RA, Miercke LJ, Stroud RM. Crystal structure of human aquaporin 4 at 1.8 A and its mechanism of conductance. Proc Natl Acad Sci U S A. 2009 May 05; 106(18):7437-42. PMID: 19383790; PMCID: PMC2678640.
    59. Alian A, DeGiovanni A, Griner SL, Finer-Moore JS, Stroud RM. Crystal structure of an RluF-RNA complex: a base-pair rearrangement is the key to selectivity of RluF for U2604 of the ribosome. J Mol Biol. 2009 May 15; 388(4):785-800. PMID: 19298824; PMCID: PMC2796871.
    60. Stroud RM, Choe S, Holton J, Kaback HR, Kwiatkowski W, Minor DL, Riek R, Sali A, Stahlberg H, Harries W. 2007 annual progress report synopsis of the Center for Structures of Membrane Proteins. J Struct Funct Genomics. 2009 Apr; 10(2):193-208. PMID: 19148774; PMCID: PMC2705707.
    61. Newby ZE, O'Connell JD, Gruswitz F, Hays FA, Harries WE, Harwood IM, Ho JD, Lee JK, Savage DF, Miercke LJ, Stroud RM. A general protocol for the crystallization of membrane proteins for X-ray structural investigation. Nat Protoc. 2009; 4(5):619-37. PMID: 19360018; PMCID: PMC4075773.
    62. Korennykh AV, Egea PF, Korostelev AA, Finer-Moore J, Zhang C, Shokat KM, Stroud RM, Walter P. The unfolded protein response signals through high-order assembly of Ire1. Nature. 2009 Feb 05; 457(7230):687-93. PMID: 19079236; PMCID: PMC2846394.
    63. Li M, Hays FA, Roe-Zurz Z, Vuong L, Kelly L, Ho CM, Robbins RM, Pieper U, O'Connell JD, Miercke LJ, Giacomini KM, Sali A, Stroud RM. Selecting optimum eukaryotic integral membrane proteins for structure determination by rapid expression and solubilization screening. J Mol Biol. 2009 Jan 23; 385(3):820-30. PMID: 19061901; PMCID: PMC2659619.
    64. Hays FA, Roe-Zurz Z, Li M, Kelly L, Gruswitz F, Sali A, Stroud RM. Ratiocinative screen of eukaryotic integral membrane protein expression and solubilization for structure determination. J Struct Funct Genomics. 2009 Mar; 10(1):9-16. PMID: 19031011; PMCID: PMC2756966.
    65. Egea PF, Tsuruta H, de Leon GP, Napetschnig J, Walter P, Stroud RM. Structures of the signal recognition particle receptor from the archaeon Pyrococcus furiosus: implications for the targeting step at the membrane. PLoS One. 2008; 3(11):e3619. PMID: 18978942.
      View in: PubMed
    66. Egea PF, Napetschnig J, Walter P, Stroud RM. Structures of SRP54 and SRP19, the two proteins that organize the ribonucleic core of the signal recognition particle from Pyrococcus furiosus. PLoS One. 2008; 3(10):e3528. PMID: 18953414.
      View in: PubMed
    67. Stroud RM. Michael A. Raftery (1936-2007)--the first enzyme mechanism, sequential cooperativity, and the nicotinic acetylcholine receptor defined. Protein Sci. 2008 Oct; 17(10):1864-6. PMID: 18819191; PMCID: PMC2548366.
    68. Lee JK, Belogrudov GI, Stroud RM. Crystal structure of bovine mitochondrial factor B at 0.96-A resolution. Proc Natl Acad Sci U S A. 2008 Sep 09; 105(36):13379-84. PMID: 18768789; PMCID: PMC2528870.
    69. Newby ZE, O'Connell J, Robles-Colmenares Y, Khademi S, Miercke LJ, Stroud RM. Crystal structure of the aquaglyceroporin PfAQP from the malarial parasite Plasmodium falciparum. Nat Struct Mol Biol. 2008 Jun; 15(6):619-25. PMID: 18500352; PMCID: PMC2568999.
    70. Alian A, Lee TT, Griner SL, Stroud RM, Finer-Moore J. Structure of a TrmA-RNA complex: A consensus RNA fold contributes to substrate selectivity and catalysis in m5U methyltransferases. Proc Natl Acad Sci U S A. 2008 May 13; 105(19):6876-81. PMID: 18451029; PMCID: PMC2383949.
    71. Du Z, Lee JK, Tjhen R, Stroud RM, James TL. Structural and biochemical insights into the dicing mechanism of mouse Dicer: a conserved lysine is critical for dsRNA cleavage. Proc Natl Acad Sci U S A. 2008 Feb 19; 105(7):2391-6. PMID: 18268334; PMCID: PMC2268147.
    72. Menuz K, Stroud RM, Nicoll RA, Hays FA. TARP auxiliary subunits switch AMPA receptor antagonists into partial agonists. Science. 2007 Nov 02; 318(5851):815-7. PMID: 17975069.
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    73. Reyes CL, Rutenber E, Walter P, Stroud RM. X-ray structures of the signal recognition particle receptor reveal targeting cycle intermediates. PLoS One. 2007 Jul 11; 2(7):e607. PMID: 17622352.
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    74. Du Z, Lee JK, Fenn S, Tjhen R, Stroud RM, James TL. X-ray crystallographic and NMR studies of protein-protein and protein-nucleic acid interactions involving the KH domains from human poly(C)-binding protein-2. RNA. 2007 Jul; 13(7):1043-51. PMID: 17526645; PMCID: PMC1894928.
    75. Savage DF, Anderson CL, Robles-Colmenares Y, Newby ZE, Stroud RM. Cell-free complements in vivo expression of the E. coli membrane proteome. Protein Sci. 2007 May; 16(5):966-76. PMID: 17456747; PMCID: PMC2206641.
    76. Hur S, Stroud RM. How U38, 39, and 40 of many tRNAs become the targets for pseudouridylation by TruA. Mol Cell. 2007 Apr 27; 26(2):189-203. PMID: 17466622; PMCID: PMC3562137.
    77. Fenn S, Du Z, Lee JK, Tjhen R, Stroud RM, James TL. Crystal structure of the third KH domain of human poly(C)-binding protein-2 in complex with a C-rich strand of human telomeric DNA at 1.6 A resolution. Nucleic Acids Res. 2007; 35(8):2651-60. PMID: 17426136; PMCID: PMC1885661.
    78. Savage DF, Stroud RM. Structural basis of aquaporin inhibition by mercury. J Mol Biol. 2007 May 04; 368(3):607-17. PMID: 17376483; PMCID: PMC3535476.
    79. Pan H, Ho JD, Stroud RM, Finer-Moore J. The crystal structure of E. coli rRNA pseudouridine synthase RluE. J Mol Biol. 2007 Apr 13; 367(5):1459-70. PMID: 17320904; PMCID: PMC1876706.
    80. Stroud RM. Transmembrane transporters: an open and closed case. Proc Natl Acad Sci U S A. 2007 Jan 30; 104(5):1445-6. PMID: 17251354; PMCID: PMC1785277.
    81. Gruswitz F, O'Connell J, Stroud RM. Inhibitory complex of the transmembrane ammonia channel, AmtB, and the cytosolic regulatory protein, GlnK, at 1.96 A. Proc Natl Acad Sci U S A. 2007 Jan 02; 104(1):42-7. PMID: 17190799; PMCID: PMC1765473.
    82. Khademi S, Stroud RM. The Amt/MEP/Rh family: structure of AmtB and the mechanism of ammonia gas conduction. Physiology (Bethesda). 2006 Dec; 21:419-29. PMID: 17119155.
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    83. Hur S, Stroud RM, Finer-Moore J. Substrate recognition by RNA 5-methyluridine methyltransferases and pseudouridine synthases: a structural perspective. J Biol Chem. 2006 Dec 22; 281(51):38969-73. PMID: 17085441.
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    84. He X, Alian A, Stroud R, Ortiz de Montellano PR. Pyrrolidine carboxamides as a novel class of inhibitors of enoyl acyl carrier protein reductase from Mycobacterium tuberculosis. J Med Chem. 2006 Oct 19; 49(21):6308-23. PMID: 17034137; PMCID: PMC2517584.
    85. Newby Z, Lee TT, Morse RJ, Liu Y, Liu L, Venkatraman P, Santi DV, Finer-Moore JS, Stroud RM. The role of protein dynamics in thymidylate synthase catalysis: variants of conserved 2'-deoxyuridine 5'-monophosphate (dUMP)-binding Tyr-261. Biochemistry. 2006 Jun 20; 45(24):7415-28. PMID: 16768437; PMCID: PMC2556892.
    86. Keatinge-Clay AT, Stroud RM. The structure of a ketoreductase determines the organization of the beta-carbon processing enzymes of modular polyketide synthases. Structure. 2006 Apr; 14(4):737-48. PMID: 16564177.
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    87. Credle JJ, Finer-Moore JS, Papa FR, Stroud RM, Walter P. On the mechanism of sensing unfolded protein in the endoplasmic reticulum. Proc Natl Acad Sci U S A. 2005 Dec 27; 102(52):18773-84. PMID: 16365312; PMCID: PMC1316886.
    88. Lee JK, Kozono D, Remis J, Kitagawa Y, Agre P, Stroud RM. Structural basis for conductance by the archaeal aquaporin AqpM at 1.68 A. Proc Natl Acad Sci U S A. 2005 Dec 27; 102(52):18932-7. PMID: 16361443; PMCID: PMC1323191.
    89. Finer-Moore JS, Anderson AC, O'Neil RH, Costi MP, Ferrari S, Krucinski J, Stroud RM. The structure of Cryptococcus neoformans thymidylate synthase suggests strategies for using target dynamics for species-specific inhibition. Acta Crystallogr D Biol Crystallogr. 2005 Oct; 61(Pt 10):1320-34. PMID: 16204883.
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    90. Du Z, Lee JK, Tjhen R, Li S, Pan H, Stroud RM, James TL. Crystal structure of the first KH domain of human poly(C)-binding protein-2 in complex with a C-rich strand of human telomeric DNA at 1.7 A. J Biol Chem. 2005 Nov 18; 280(46):38823-30. PMID: 16186123.
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    91. Egea PF, Stroud RM, Walter P. Targeting proteins to membranes: structure of the signal recognition particle. Curr Opin Struct Biol. 2005 Apr; 15(2):213-20. PMID: 15837181.
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    92. Lee TT, Agarwalla S, Stroud RM. A unique RNA Fold in the RumA-RNA-cofactor ternary complex contributes to substrate selectivity and enzymatic function. Cell. 2005 Mar 11; 120(5):599-611. PMID: 15766524.
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    93. Laporte SL, Forsyth CM, Cunningham BC, Miercke LJ, Akhavan D, Stroud RM. De novo design of an IL-4 antagonist and its structure at 1.9 A. Proc Natl Acad Sci U S A. 2005 Feb 08; 102(6):1889-94. PMID: 15684085; PMCID: PMC548554.
    94. Chu F, Shan SO, Moustakas DT, Alber F, Egea PF, Stroud RM, Walter P, Burlingame AL. Unraveling the interface of signal recognition particle and its receptor by using chemical cross-linking and tandem mass spectrometry. Proc Natl Acad Sci U S A. 2004 Nov 23; 101(47):16454-9. PMID: 15546976; PMCID: PMC528904.
    95. Shan SO, Stroud RM, Walter P. Mechanism of association and reciprocal activation of two GTPases. PLoS Biol. 2004 Oct; 2(10):e320. PMID: 15383838; PMCID: PMC517823.
    96. Harries WE, Akhavan D, Miercke LJ, Khademi S, Stroud RM. The channel architecture of aquaporin 0 at a 2.2-A resolution. Proc Natl Acad Sci U S A. 2004 Sep 28; 101(39):14045-50. PMID: 15377788; PMCID: PMC521118.
    97. Khademi S, O'Connell J, Remis J, Robles-Colmenares Y, Miercke LJ, Stroud RM. Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 A. Science. 2004 Sep 10; 305(5690):1587-94. PMID: 15361618.
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    98. Keatinge-Clay AT, Maltby DA, Medzihradszky KF, Khosla C, Stroud RM. An antibiotic factory caught in action. Nat Struct Mol Biol. 2004 Sep; 11(9):888-93. PMID: 15286722.
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    99. Agarwalla S, Stroud RM, Gaffney BJ. Redox reactions of the iron-sulfur cluster in a ribosomal RNA methyltransferase, RumA: optical and EPR studies. J Biol Chem. 2004 Aug 13; 279(33):34123-9. PMID: 15181002; PMCID: PMC1237038.
    100. Stroud RM, Wells JA. Mechanistic diversity of cytokine receptor signaling across cell membranes. Sci STKE. 2004 Apr 28; 2004(231):re7. PMID: 15126678.
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    101. Lee TT, Agarwalla S, Stroud RM. Crystal structure of RumA, an iron-sulfur cluster containing E. coli ribosomal RNA 5-methyluridine methyltransferase. Structure. 2004 Mar; 12(3):397-407. PMID: 15016356.
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    102. Egea PF, Shan SO, Napetschnig J, Savage DF, Walter P, Stroud RM. Substrate twinning activates the signal recognition particle and its receptor. Nature. 2004 Jan 15; 427(6971):215-21. PMID: 14724630.
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    103. Savage DF, Egea PF, Robles-Colmenares Y, O'Connell JD, Stroud RM. Architecture and selectivity in aquaporins: 2.5 a X-ray structure of aquaporin Z. PLoS Biol. 2003 Dec; 1(3):E72. PMID: 14691544; PMCID: PMC300682.
    104. Foster PG, Nunes CR, Greene P, Moustakas D, Stroud RM. The first structure of an RNA m5C methyltransferase, Fmu, provides insight into catalytic mechanism and specific binding of RNA substrate. Structure. 2003 Dec; 11(12):1609-20. PMID: 14656444.
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    105. Watanabe K, Khosla C, Stroud RM, Tsai SC. Crystal structure of an Acyl-ACP dehydrogenase from the FK520 polyketide biosynthetic pathway: insights into extender unit biosynthesis. J Mol Biol. 2003 Nov 28; 334(3):435-44. PMID: 14623185.
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    106. Lee JK, Khademi S, Harries W, Savage D, Miercke L, Stroud RM. Water and glycerol permeation through the glycerol channel GlpF and the aquaporin family. J Synchrotron Radiat. 2004 Jan 01; 11(Pt 1):86-8. PMID: 14646142.
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    107. Stroud RM, Savage D, Miercke LJ, Lee JK, Khademi S, Harries W. Selectivity and conductance among the glycerol and water conducting aquaporin family of channels. FEBS Lett. 2003 Nov 27; 555(1):79-84. PMID: 14630323.
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    108. Pan H, Agarwalla S, Moustakas DT, Finer-Moore J, Stroud RM. Structure of tRNA pseudouridine synthase TruB and its RNA complex: RNA recognition through a combination of rigid docking and induced fit. Proc Natl Acad Sci U S A. 2003 Oct 28; 100(22):12648-53. PMID: 14566049; PMCID: PMC240672.
    109. O'Neil RH, Lilien RH, Donald BR, Stroud RM, Anderson AC. Phylogenetic classification of protozoa based on the structure of the linker domain in the bifunctional enzyme, dihydrofolate reductase-thymidylate synthase. J Biol Chem. 2003 Dec 26; 278(52):52980-7. PMID: 14555647.
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    110. Gonzalez-Pacanowska D, Ruiz-Perez LM, Carreras-Gómez MA, Costi MP, Stroud RM, Finer-Moore J, Santi DV. The structural roles of conserved Pro196, Pro197 and His199 in the mechanism of thymidylate synthase. Protein Eng. 2003 Aug; 16(8):607-14. PMID: 12968078.
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    111. Stroud RM, Miercke LJ, O'Connell J, Khademi S, Lee JK, Remis J, Harries W, Robles Y, Akhavan D. Glycerol facilitator GlpF and the associated aquaporin family of channels. Curr Opin Struct Biol. 2003 Aug; 13(4):424-31. PMID: 12948772.
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    112. Jez JM, Chen JC, Rastelli G, Stroud RM, Santi DV. Crystal structure and molecular modeling of 17-DMAG in complex with human Hsp90. Chem Biol. 2003 Apr; 10(4):361-8. PMID: 12725864.
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    113. Reiling KK, Krucinski J, Miercke LJ, Raymond WW, Caughey GH, Stroud RM. Structure of human pro-chymase: a model for the activating transition of granule-associated proteases. Biochemistry. 2003 Mar 11; 42(9):2616-24. PMID: 12614156.
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    114. Birdsall DL, Finer-Moore J, Stroud RM. The only active mutant of thymidylate synthase D169, a residue far from the site of methyl transfer, demonstrates the exquisite nature of enzyme specificity. Protein Eng. 2003 Mar; 16(3):229-40. PMID: 12702803.
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    115. Keatinge-Clay AT, Shelat AA, Savage DF, Tsai SC, Miercke LJ, O'Connell JD, Khosla C, Stroud RM. Catalysis, specificity, and ACP docking site of Streptomyces coelicolor malonyl-CoA:ACP transacylase. Structure. 2003 Feb; 11(2):147-54. PMID: 12575934.
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    116. Finer-Moore JS, Santi DV, Stroud RM. Lessons and conclusions from dissecting the mechanism of a bisubstrate enzyme: thymidylate synthase mutagenesis, function, and structure. Biochemistry. 2003 Jan 21; 42(2):248-56. PMID: 12525151.
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    117. Stroud RM, Finer-Moore JS. Conformational dynamics along an enzymatic reaction pathway: thymidylate synthase, "the movie". Biochemistry. 2003 Jan 21; 42(2):239-47. PMID: 12525150.
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    118. O'Neil RH, Lilien RH, Donald BR, Stroud RM, Anderson AC. The crystal structure of dihydrofolate reductase-thymidylate synthase from Cryptosporidium hominis reveals a novel architecture for the bifunctional enzyme. J Eukaryot Microbiol. 2003; 50 Suppl:555-6. PMID: 14736160.
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    119. Stroud RM, Nollert P, Miercke L. The glycerol facilitator GlpF its aquaporin family of channels, and their selectivity. Adv Protein Chem. 2003; 63:291-316. PMID: 12629974.
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    120. Pan H, Tsai Sc, Meadows ES, Miercke LJ, Keatinge-Clay AT, O'Connell J, Khosla C, Stroud RM. Crystal structure of the priming beta-ketosynthase from the R1128 polyketide biosynthetic pathway. Structure. 2002 Nov; 10(11):1559-68. PMID: 12429097.
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    121. Tsai SC, Lu H, Cane DE, Khosla C, Stroud RM. Insights into channel architecture and substrate specificity from crystal structures of two macrocycle-forming thioesterases of modular polyketide synthases. Biochemistry. 2002 Oct 22; 41(42):12598-606. PMID: 12379102.
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    122. Ramirez UD, Minasov G, Focia PJ, Stroud RM, Walter P, Kuhn P, Freymann DM. Structural basis for mobility in the 1.1 A crystal structure of the NG domain of Thermus aquaticus Ffh. J Mol Biol. 2002 Jul 19; 320(4):783-99. PMID: 12095255; PMCID: PMC3542393.
    123. Costi MP, Tondi D, Rinaldi M, Barlocco D, Pecorari P, Soragni F, Venturelli A, Stroud RM. Structure-based studies on species-specific inhibition of thymidylate synthase. Biochim Biophys Acta. 2002 Jul 18; 1587(2-3):206-14. PMID: 12084462.
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    124. Fritz TA, Liu L, Finer-Moore JS, Stroud RM. Tryptophan 80 and leucine 143 are critical for the hydride transfer step of thymidylate synthase by controlling active site access. Biochemistry. 2002 Jun 04; 41(22):7021-9. PMID: 12033935.
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    125. Tajkhorshid E, Nollert P, Jensen MØ, Miercke LJ, O'Connell J, Stroud RM, Schulten K. Control of the selectivity of the aquaporin water channel family by global orientational tuning. Science. 2002 Apr 19; 296(5567):525-30. PMID: 11964478.
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    126. Stroud RM, Nollert P, Miercke LJW, Harries WEC, O'Connell J. Encoding Selectivity of a Transmembrane Channel. ScientificWorldJournal. 2002; 2:111. PMID: 29973827.
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    127. Reiling KK, Endres NF, Dauber DS, Craik CS, Stroud RM. Anisotropic dynamics of the JE-2147-HIV protease complex: drug resistance and thermodynamic binding mode examined in a 1.09 A structure. Biochemistry. 2002 Apr 09; 41(14):4582-94. PMID: 11926820.
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    128. Agarwalla S, Kealey JT, Santi DV, Stroud RM. Characterization of the 23 S ribosomal RNA m5U1939 methyltransferase from Escherichia coli. J Biol Chem. 2002 Mar 15; 277(11):8835-40. PMID: 11779873.
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    129. Fu D, Libson A, Stroud R. The structure of GlpF, a glycerol conducting channel. Novartis Found Symp. 2002; 245:51-61; discussion 61-5, 165-8. PMID: 12027015.
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    130. Chen JC, Krucinski J, Miercke LJ, Finer-Moore JS, Tang AH, Leavitt AD, Stroud RM. Crystal structure of the HIV-1 integrase catalytic core and C-terminal domains: a model for viral DNA binding. Proc Natl Acad Sci U S A. 2000 Jul 18; 97(15):8233-8. PMID: 10890912; PMCID: PMC26930.
    131. Stroud RM, Walter P. Signal sequence recognition and protein targeting. Curr Opin Struct Biol. 1999 Dec; 9(6):754-9. PMID: 10607673.
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    132. Stroud RM, Reiling K, Wiener M, Freymann D. Ion-channel-forming colicins. Curr Opin Struct Biol. 1998 Aug; 8(4):525-33. PMID: 9729746.
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    133. Rutenber EE, Stroud RM. Binding of the anticancer drug ZD1694 to E. coli thymidylate synthase: assessing specificity and affinity. Structure. 1996 Nov 15; 4(11):1317-24. PMID: 8939755.
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    134. Stroud RM, Fauman EB. Significance of structural changes in proteins: expected errors in refined protein structures. Protein Sci. 1995 Nov; 4(11):2392-404. PMID: 8563637; PMCID: PMC2143015.
    135. Vassarotti A, Stroud R, Douglas M. Independent mutations at the amino terminus of a protein act as surrogate signals for mitochondrial import. EMBO J. 1987 Mar; 6(3):705-11. PMID: 2884105; PMCID: PMC553454.
    136. Hershenson S, Helmers N, Desmueles P, Stroud R. Purification and crystallization of creatine kinase from rabbit skeletal muscle. J Biol Chem. 1986 Mar 15; 261(8):3732-6. PMID: 3949787.
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