Nico Stuurman, PhD

Title(s)Associate Adjunct Professor, Biochemistry and Biophysics
SchoolSchool of Medicine
Address35 Medical Center Way, #S252
San Francisco CA 94143
PronounsHe/Him/His
ORCID ORCID Icon0000-0002-6179-8613 Additional info
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    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help. to make corrections and additions.
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    1. Nucleolar dynamics are determined by the ordered assembly of the ribosome. bioRxiv. 2023 Sep 27. Sheu-Gruttadauria J, Yan X, Stuurman N, Floor SN, Vale RD. PMID: 37808656; PMCID: PMC10557630.
      View in: PubMed   Mentions:
    2. Three-color single-molecule imaging reveals conformational dynamics of dynein undergoing motility. Proc Natl Acad Sci U S A. 2021 08 03; 118(31). Niekamp S, Stuurman N, Zhang N, Vale RD. PMID: 34326255; PMCID: PMC8346880.
      View in: PubMed   Mentions: 5     Fields:    Translation:Cells
    3. Pycro-Manager: open-source software for customized and reproducible microscope control. Nat Methods. 2021 03; 18(3):226-228. Pinkard H, Stuurman N, Ivanov IE, Anthony NM, Ouyang W, Li B, Yang B, Tsuchida MA, Chhun B, Zhang G, Mei R, Anderson M, Shepherd DP, Hunt-Isaak I, Dunn RL, Jahr W, Kato S, Royer LA, Thiagarajah JR, Eliceiri KW, Lundberg E, Mehta SB, Waller L. PMID: 33674797; PMCID: PMC8532176.
      View in: PubMed   Mentions: 25     Fields:    
    4. High-content imaging-based pooled CRISPR screens in mammalian cells. J Cell Biol. 2021 02 01; 220(2). Yan X, Stuurman N, Ribeiro SA, Tanenbaum ME, Horlbeck MA, Liem CR, Jost M, Weissman JS, Vale RD. PMID: 33465779; PMCID: PMC7821101.
      View in: PubMed   Mentions: 30     Fields:    Translation:HumansCells
    5. A 6-nm ultra-photostable DNA FluoroCube for fluorescence imaging. Nat Methods. 2020 04; 17(4):437-441. Niekamp S, Stuurman N, Vale RD. PMID: 32203385; PMCID: PMC7138518.
      View in: PubMed   Mentions: 12     Fields:    Translation:Humans
    6. Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution. Nat Methods. 2019 06; 16(6):501-504. Yang B, Chen X, Wang Y, Feng S, Pessino V, Stuurman N, Cho NH, Cheng KW, Lord SJ, Xu L, Xie D, Mullins RD, Leonetti MD, Huang B. PMID: 31061492; PMCID: PMC6557432.
      View in: PubMed   Mentions: 51     Fields:    Translation:HumansAnimalsCells
    7. Nanometer-accuracy distance measurements between fluorophores at the single-molecule level. Proc Natl Acad Sci U S A. 2019 03 05; 116(10):4275-4284. Niekamp S, Sung J, Huynh W, Bhabha G, Vale RD, Stuurman N. PMID: 30770448; PMCID: PMC6410877.
      View in: PubMed   Mentions: 13     Fields:    Translation:Cells
    8. Cellular aspect ratio and cell division mechanics underlie the patterning of cell progeny in diverse mammalian epithelia. Elife. 2018 06 13; 7. McKinley KL, Stuurman N, Royer LA, Schartner C, Castillo-Azofeifa D, Delling M, Klein OD, Vale RD. PMID: 29897330; PMCID: PMC6023609.
      View in: PubMed   Mentions: 27     Fields:    Translation:AnimalsCells
    9. Visualizing Calcium Flux in Freely Moving Nematode Embryos. Biophys J. 2017 May 09; 112(9):1975-1983. Ardiel EL, Kumar A, Marbach J, Christensen R, Gupta R, Duncan W, Daniels JS, Stuurman N, Colón-Ramos D, Shroff H. PMID: 28494967; PMCID: PMC5425378.
      View in: PubMed   Mentions: 19     Fields:    Translation:AnimalsCells
    10. An acquisition and analysis pipeline for scanning angle interference microscopy. Nat Methods. 2016 10 31; 13(11):897-898. Carbone CB, Vale RD, Stuurman N. PMID: 27798596; PMCID: PMC5100979.
      View in: PubMed   Mentions: 6     Fields:    Translation:Humans
    11. Micro-Magellan: open-source, sample-adaptive, acquisition software for optical microscopy. Nat Methods. 2016 09 29; 13(10):807-809. Pinkard H, Stuurman N, Corbin K, Vale R, Krummel MF. PMID: 27684577; PMCID: PMC5100821.
      View in: PubMed   Mentions: 10     Fields:    Translation:Humans
    12. Impact of New Camera Technologies on Discoveries in Cell Biology. Biol Bull. 2016 08; 231(1):5-13. Stuurman N, Vale RD. PMID: 27638691; PMCID: PMC5100698.
      View in: PubMed   Mentions: 6     Fields:    
    13. Quantitative evaluation of software packages for single-molecule localization microscopy. Nat Methods. 2015 Aug; 12(8):717-24. Sage D, Kirshner H, Pengo T, Stuurman N, Min J, Manley S, Unser M. PMID: 26076424.
      View in: PubMed   Mentions: 122     Fields:    Translation:AnimalsCells
    14. High-resolution imaging of cardiomyocyte behavior reveals two distinct steps in ventricular trabeculation. Development. 2014 Feb; 141(3):585-93. Staudt DW, Liu J, Thorn KS, Stuurman N, Liebling M, Stainier DY. PMID: 24401373; PMCID: PMC3899815.
      View in: PubMed   Mentions: 65     Fields:    Translation:AnimalsCells
    15. Advanced methods of microscope control using μManager software. J Biol Methods. 2014; 1(2). Edelstein AD, Tsuchida MA, Amodaj N, Pinkard H, Vale RD, Stuurman N. PMID: 25606571; PMCID: PMC4297649.
      View in: PubMed   Mentions: 790  
    16. Genes involved in centrosome-independent mitotic spindle assembly in Drosophila S2 cells. Proc Natl Acad Sci U S A. 2013 Dec 03; 110(49):19808-13. Moutinho-Pereira S, Stuurman N, Afonso O, Hornsveld M, Aguiar P, Goshima G, Vale RD, Maiato H. PMID: 24255106; PMCID: PMC3856817.
      View in: PubMed   Mentions: 31     Fields:    Translation:AnimalsCells
    17. Chromophore-assisted laser inactivation in neural development. Neurosci Bull. 2012 Aug; 28(4):333-41. Li W, Stuurman N, Ou G. PMID: 22833033; PMCID: PMC5561893.
      View in: PubMed   Mentions: 2     Fields:    Translation:HumansAnimalsCells
    18. Biological imaging software tools. Nat Methods. 2012 Jun 28; 9(7):697-710. Eliceiri KW, Berthold MR, Goldberg IG, Ibáñez L, Manjunath BS, Martone ME, Murphy RF, Peng H, Plant AL, Roysam B, Stuurman N, Stuurmann N, Swedlow JR, Tomancak P, Carpenter AE. PMID: 22743775; PMCID: PMC3659807.
      View in: PubMed   Mentions: 206     Fields:    
    19. Software tools, data structures, and interfaces for microscope imaging. Cold Spring Harb Protoc. 2012 Jan 01; 2012(1):50-61. Stuurman N, Swedlow JR. PMID: 22194261.
      View in: PubMed   Mentions: 3     Fields:    
    20. Computer control of microscopes using µManager. Curr Protoc Mol Biol. 2010 Oct; Chapter 14:Unit14.20. Edelstein A, Amodaj N, Hoover K, Vale R, Stuurman N. PMID: 20890901; PMCID: PMC3065365.
      View in: PubMed   Mentions: 893     Fields:    
    21. Polarized myosin produces unequal-size daughters during asymmetric cell division. Science. 2010 Oct 29; 330(6004):677-80. Ou G, Stuurman N, D'Ambrosio M, Vale RD. PMID: 20929735; PMCID: PMC3032534.
      View in: PubMed   Mentions: 78     Fields:    Translation:AnimalsCells
    22. Imaging single molecular motor motility with total internal reflection fluorescence microscopy (TIRFM). Cold Spring Harb Protoc. 2010 Mar; 2010(3):pdb.prot5399. Reck-Peterson SL, Derr ND, Stuurman N. PMID: 20194468.
      View in: PubMed   Mentions: 1     Fields:    Translation:Cells
    23. Imaging single molecules using total internal reflection fluorescence microscopy (TIRFM). Cold Spring Harb Protoc. 2010 Mar; 2010(3):pdb.top73. Reck-Peterson SL, Derr ND, Stuurman N. PMID: 20194477.
      View in: PubMed   Mentions: 13     Fields:    Translation:Cells
    24. Determining single-molecule intensity as a function of power density. Cold Spring Harb Protoc. 2010 Mar; 2010(3):pdb.prot5398. Reck-Peterson SL, Derr ND, Stuurman N. PMID: 20194467.
      View in: PubMed   Mentions:    Fields:    
    25. Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle. J Cell Biol. 2008 May 05; 181(3):421-9. Goshima G, Mayer M, Zhang N, Stuurman N, Vale RD. PMID: 18443220; PMCID: PMC2364697.
      View in: PubMed   Mentions: 204     Fields:    Translation:HumansAnimalsCells
    26. Functional genomic screen reveals genes involved in lipid-droplet formation and utilization. Nature. 2008 May 29; 453(7195):657-61. Guo Y, Walther TC, Rao M, Stuurman N, Goshima G, Terayama K, Wong JS, Vale RD, Walter P, Farese RV. PMID: 18408709; PMCID: PMC2734507.
      View in: PubMed   Mentions: 335     Fields:    Translation:AnimalsCells
    27. High throughput microscopy: from raw images to discoveries. J Cell Sci. 2007 Nov 01; 120(Pt 21):3715-22. Wollman R, Stuurman N. PMID: 17959627.
      View in: PubMed   Mentions: 27     Fields:    Translation:HumansCells
    28. Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore. J Cell Biol. 2007 Jun 18; 177(6):1005-15. Griffis ER, Stuurman N, Vale RD. PMID: 17576797; PMCID: PMC2064361.
      View in: PubMed   Mentions: 132     Fields:    Translation:AnimalsCells
    29. Genes required for mitotic spindle assembly in Drosophila S2 cells. Science. 2007 Apr 20; 316(5823):417-21. Goshima G, Wollman R, Goodwin SS, Zhang N, Scholey JM, Vale RD, Stuurman N. PMID: 17412918; PMCID: PMC2837481.
      View in: PubMed   Mentions: 309     Fields:    Translation:AnimalsCells
    30. Single-molecule observations of neck linker conformational changes in the kinesin motor protein. Nat Struct Mol Biol. 2006 Oct; 13(10):887-94. Tomishige M, Stuurman N, Vale RD. PMID: 17013387.
      View in: PubMed   Mentions: 40     Fields:    Translation:Cells
    31. Length control of the metaphase spindle. Curr Biol. 2005 Nov 22; 15(22):1979-88. Goshima G, Wollman R, Stuurman N, Scholey JM, Vale RD. PMID: 16303556.
      View in: PubMed   Mentions: 138     Fields:    Translation:AnimalsCells
    32. Use of RNA interference in Drosophila S2 cells to identify host pathways controlling compartmentalization of an intracellular pathogen. Proc Natl Acad Sci U S A. 2005 Sep 20; 102(38):13646-51. Cheng LW, Viala JP, Stuurman N, Wiedemann U, Vale RD, Portnoy DA. PMID: 16157870; PMCID: PMC1224656.
      View in: PubMed   Mentions: 57     Fields:    Translation:AnimalsCells
    33. Distinct pathways control recruitment and maintenance of myosin II at the cleavage furrow during cytokinesis. Proc Natl Acad Sci U S A. 2005 Sep 20; 102(38):13473-8. Dean SO, Rogers SL, Stuurman N, Vale RD, Spudich JA. PMID: 16174742; PMCID: PMC1200093.
      View in: PubMed   Mentions: 54     Fields:    Translation:AnimalsCells
    34. Molecular requirements for actin-based lamella formation in Drosophila S2 cells. J Cell Biol. 2003 Sep 15; 162(6):1079-88. Rogers SL, Wiedemann U, Stuurman N, Vale RD. PMID: 12975351; PMCID: PMC2172842.
      View in: PubMed   Mentions: 207     Fields:    Translation:AnimalsCells
    35. Mitochondrial positioning in fission yeast is driven by association with dynamic microtubules and mitotic spindle poles. Proc Natl Acad Sci U S A. 2003 Sep 30; 100(20):11424-8. Yaffe MP, Stuurman N, Vale RD. PMID: 12972644; PMCID: PMC208773.
      View in: PubMed   Mentions: 32     Fields:    Translation:AnimalsCells
    36. Role of phosphatidylinositol(4,5)bisphosphate organization in membrane transport by the Unc104 kinesin motor. Cell. 2002 May 03; 109(3):347-58. Klopfenstein DR, Tomishige M, Stuurman N, Vale RD. PMID: 12015984; PMCID: PMC2851634.
      View in: PubMed   Mentions: 129     Fields:    Translation:AnimalsCells
    37. Use of green fluorescent protein color variants expressed on stable broad-host-range vectors to visualize rhizobia interacting with plants. Mol Plant Microbe Interact. 2000 Nov; 13(11):1163-9. Stuurman N, Pacios Bras C, Schlaman HR, Wijfjes AH, Bloemberg G, Spaink HP. PMID: 11059482.
      View in: PubMed   Mentions: 35     Fields:    Translation:Cells
    38. Simultaneous imaging of Pseudomonas fluorescens WCS365 populations expressing three different autofluorescent proteins in the rhizosphere: new perspectives for studying microbial communities. Mol Plant Microbe Interact. 2000 Nov; 13(11):1170-6. Bloemberg GV, Wijfjes AH, Lamers GE, Stuurman N, Lugtenberg BJ. PMID: 11059483.
      View in: PubMed   Mentions: 57     Fields:    Translation:AnimalsCellsPHPublic Health
    39. B-type granule containing protrusions and interconnections between amyloplasts in developing wheat endosperm revealed by transmission electron microscopy and GFP expression. J Exp Bot. 2000 Aug; 51(349):1357-61. Langeveld SM, van Wijk R, Stuurman N, Kijne JW, de Pater S. PMID: 10944148.
      View in: PubMed   Mentions: 29     Fields:    Translation:AnimalsCells
    40. A Lotus japonicus nodulation system based on heterologous expression of the fucosyl transferase NodZ and the acetyl transferase NoIL in Rhizobium leguminosarum. Mol Plant Microbe Interact. 2000 Apr; 13(4):475-9. Pacios Bras C, Jordá MA, Wijfjes AH, Harteveld M, Stuurman N, Thomas-Oates JE, Spaink HP. PMID: 10755312.
      View in: PubMed   Mentions: 18     Fields:    Translation:AnimalsCells
    41. Ectopic overexpression of Drosophila lamin C is stage-specific lethal. Exp Cell Res. 1999 May 01; 248(2):350-7. Stuurman N, Delbecque JP, Callaerts P, Aebi U. PMID: 10222127.
      View in: PubMed   Mentions: 9     Fields:    Translation:Animals
    42. Phosphorylation of the major Drosophila lamin in vivo: site identification during both M-phase (meiosis) and interphase by electrospray ionization tandem mass spectrometry. Biochemistry. 1999 Apr 06; 38(14):4620-32. Schneider U, Mini T, Jenö P, Fisher PA, Stuurman N. PMID: 10194384.
      View in: PubMed   Mentions: 11     Fields:    Translation:HumansAnimalsCells
    43. Interactions between coiled-coil proteins: Drosophila lamin Dm0 binds to the bicaudal-D protein. Eur J Cell Biol. 1999 Apr; 78(4):278-87. Stuurman N, Häner M, Sasse B, Hübner W, Suter B, Aebi U. PMID: 10350216.
      View in: PubMed   Mentions: 21     Fields:    Translation:AnimalsCells
    44. Assembly of Drosophila lamin Dm0 and C mutant proteins studied with the baculovirus system. Eur J Cell Biol. 1998 Dec; 77(4):276-83. Krohne G, Stuurman N, Kempf A. PMID: 9930652.
      View in: PubMed   Mentions:    Fields:    Translation:AnimalsCells
    45. A tailless Drosophila lamin Dm0 fragment reveals lateral associations of dimers. J Struct Biol. 1998 Sep; 123(1):56-66. Sasse B, Aebi U, Stuurman N. PMID: 9774545.
      View in: PubMed   Mentions: 11     Fields:    Translation:AnimalsCells
    46. Interactions among Drosophila nuclear envelope proteins lamin, otefin, and YA. Mol Cell Biol. 1998 Jul; 18(7):4315-23. Goldberg M, Lu H, Stuurman N, Ashery-Padan R, Weiss AM, Yu J, Bhattacharyya D, Fisher PA, Gruenbaum Y, Wolfner MF. PMID: 9632815; PMCID: PMC109015.
      View in: PubMed   Mentions: 17     Fields:    Translation:AnimalsCells
    47. Nuclear lamins: their structure, assembly, and interactions. J Struct Biol. 1998; 122(1-2):42-66. Stuurman N, Heins S, Aebi U. PMID: 9724605.
      View in: PubMed   Mentions: 181     Fields:    Translation:HumansAnimalsCells
    48. In vivo association of lamins with nucleic acids in Drosophila melanogaster. J Cell Sci. 1998 Jan; 111 ( Pt 1):121-9. Rzepecki R, Bogachev SS, Kokoza E, Stuurman N, Fisher PA. PMID: 9394018.
      View in: PubMed   Mentions: 18     Fields:    Translation:AnimalsCells
    49. In vitro assembly of Drosophila lamin Dm0--lamin polymerization properties are conserved. Eur J Biochem. 1997 Nov 15; 250(1):30-8. Sasse B, Lustig A, Aebi U, Stuurman N. PMID: 9431987.
      View in: PubMed   Mentions: 8     Fields:    Translation:AnimalsCells
    50. Assembly of A- and B-type lamins studied in vivo with the baculovirus system. J Cell Sci. 1997 Oct; 110 ( Pt 20):2519-32. Klapper M, Exner K, Kempf A, Gehrig C, Stuurman N, Fisher PA, Krohne G. PMID: 9372441.
      View in: PubMed   Mentions: 19     Fields:    Translation:AnimalsCells
    51. Identification of a conserved phosphorylation site modulating nuclear lamin polymerization. FEBS Lett. 1997 Jan 20; 401(2-3):171-4. Stuurman N. PMID: 9013881.
      View in: PubMed   Mentions: 12     Fields:    Translation:HumansAnimalsCells
    52. Intermediate filament protein polymerization: molecular analysis of Drosophila nuclear lamin head-to-tail binding. J Struct Biol. 1996 Jul-Aug; 117(1):1-15. Stuurman N, Sasse B, Fisher PA. PMID: 8776884.
      View in: PubMed   Mentions: 25     Fields:    Translation:HumansAnimalsCells
    53. DNA from Drosophila melanogaster beta-heterochromatin binds specifically to nuclear lamins in vitro and the nuclear envelope in situ. Gene. 1996 Jun 01; 171(2):171-6. Baricheva EA, Berrios M, Bogachev SS, Borisevich IV, Lapik ER, Sharakhov IV, Stuurman N, Fisher PA. PMID: 8666268.
      View in: PubMed   Mentions: 21     Fields:    Translation:AnimalsCells
    54. Binding of matrix attachment regions to nuclear lamin is mediated by the rod domain and depends on the lamin polymerization state. FEBS Lett. 1996 Feb 12; 380(1-2):161-4. Zhao K, Harel A, Stuurman N, Guedalia D, Gruenbaum Y. PMID: 8603728.
      View in: PubMed   Mentions: 23     Fields:    Translation:AnimalsCells
    55. Expression of Drosophila lamin C is developmentally regulated: analogies with vertebrate A-type lamins. J Cell Sci. 1995 Oct; 108 ( Pt 10):3189-98. Riemer D, Stuurman N, Berrios M, Hunter C, Fisher PA, Weber K. PMID: 7593280.
      View in: PubMed   Mentions: 63     Fields:    Translation:Animals
    56. Interphase phosphorylation of the Drosophila nuclear lamin: site-mapping using a monoclonal antibody. J Cell Sci. 1995 Sep; 108 ( Pt 9):3137-44. Stuurman N, Maus N, Fisher PA. PMID: 8537453.
      View in: PubMed   Mentions: 30     Fields:    Translation:AnimalsCells
    57. Disassembly of the Drosophila nuclear lamina in a homologous cell-free system. J Cell Sci. 1995 May; 108 ( Pt 5):2027-35. Maus N, Stuurman N, Fisher PA. PMID: 7657722.
      View in: PubMed   Mentions: 3     Fields:    Translation:AnimalsCells
    58. The t(15;17) translocation alters a nuclear body in a retinoic acid-reversible fashion. EMBO J. 1994 Mar 01; 13(5):1073-83. Koken MH, Puvion-Dutilleul F, Guillemin MC, Viron A, Linares-Cruz G, Stuurman N, de Jong L, Szostecki C, Calvo F, Chomienne C, et al. PMID: 8131741; PMCID: PMC394915.
      View in: PubMed   Mentions: 129     Fields:    Translation:HumansAnimalsCells
    59. Intranuclear filaments containing a nuclear pore complex protein. J Cell Biol. 1993 Dec; 123(6 Pt 1):1333-44. Cordes VC, Reidenbach S, Köhler A, Stuurman N, van Driel R, Franke WW. PMID: 8253834; PMCID: PMC2290899.
      View in: PubMed   Mentions: 56     Fields:    Translation:AnimalsCells
    60. Induction of nuclear lamins A/C during in vitro-induced differentiation of F9 and P19 embryonal carcinoma cells. Exp Cell Res. 1992 Dec; 203(2):449-55. Mattia E, Hoff WD, den Blaauwen J, Meijne AM, Stuurman N, van Renswoude J. PMID: 1281113.
      View in: PubMed   Mentions:    Fields:    Translation:AnimalsCells
    61. Nuclear frameworks: concepts and operational definitions. Cell Biol Int Rep. 1992 Aug; 16(8):837-52. Stuurman N, de Jong L, van Driel R. PMID: 1446353.
      View in: PubMed   Mentions: 4     Fields:    Translation:Cells
    62. Three-dimensional immunogold labeling of nuclear matrix proteins in permeabilized cells. Cell Biol Int Rep. 1992 Aug; 16(8):827-36. de Graaf A, Humbel BM, Stuurman N, van Bergen en Henegouwen PM, Verkleij AJ. PMID: 1446352.
      View in: PubMed   Mentions: 2     Fields:    Translation:HumansCells
    63. Stabilization of the nuclear matrix by disulfide bridges: identification of matrix polypeptides that form disulfides. Exp Cell Res. 1992 Jun; 200(2):285-94. Stuurman N, Floore A, Colen A, de Jong L, van Driel R. PMID: 1572396.
      View in: PubMed   Mentions: 6     Fields:    Translation:AnimalsCells
    64. A monoclonal antibody recognizing nuclear matrix-associated nuclear bodies. J Cell Sci. 1992 Apr; 101 ( Pt 4):773-84. Stuurman N, de Graaf A, Floore A, Josso A, Humbel B, de Jong L, van Driel R. PMID: 1527179.
      View in: PubMed   Mentions: 104     Fields:    Translation:HumansAnimalsCells
    65. Principles of nuclear organization. Cell Biol Int Rep. 1990 Dec; 14(12):1051-74. de Jong L, van Driel R, Stuurman N, Meijne AM, van Renswoude J. PMID: 2083425.
      View in: PubMed   Mentions: 5     Fields:    Translation:Cells
    66. The nuclear matrix from cells of different origin. Evidence for a common set of matrix proteins. J Biol Chem. 1990 Apr 05; 265(10):5460-5. Stuurman N, Meijne AM, van der Pol AJ, de Jong L, van Driel R, van Renswoude J. PMID: 2180926.
      View in: PubMed   Mentions: 25     Fields:    Translation:HumansAnimalsCells
    67. The protein composition of the nuclear matrix of murine P19 embryonal carcinoma cells is differentiation-stage dependent. Exp Cell Res. 1989 Feb; 180(2):460-6. Stuurman N, Van Driel R, De Jong L, Meijne AM, Van Renswoude J. PMID: 2914580.
      View in: PubMed   Mentions: 9     Fields:    Translation:AnimalsCells
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