Bo Huang, PhD

Title(s)Professor, Pharmaceutical Chemistry
SchoolSchool of Pharmacy
Address1700 4th St, #303A
San Francisco CA 94158
ORCID ORCID Icon0000-0003-1704-4141 Additional info
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    Collapse Biography 
    Collapse Awards and Honors
    2017UCSF Byers Award for Basic Science
    2016American Society for Cell Biology Young Life Scientist Award
    2011NIH Director's New Innovator Award
    2010Searle Scholar
    2010Packard Fellowship for Science and Engineering
    2007GE & Science Young Life Scientist Award, North America Regional Winner
    2001  - 2004Stanford Graduate Fellowship
    2006Annual Reviews Prize in Physical Chemistry, Stanford University

    Collapse Overview 
    Collapse Overview
    Cellular processes are carried out by coordinated participation of many biomolecules in a tiny volume. Many people have been dreaming to see clear pictures of these processes in order to understand how these molecules work together. Taking on this challenge, we are developing new visualization techniques and imaging probes by combining super-resolution microscopy, protein engineering and microfluidic automation. We are particularly interested in the following problems:

    (1) Physical organization and dynamics of the genome,
    (2) Architecture of large protein complexes such as the centrosome, and
    (3) Spatial distribution of membrane proteins, particularly G-protein coupled receptors and neuron adhesion molecules, and how this distribution defines their signaling specificity.

    In order to study these systems, we are developing the following microscopy technologies:

    (1) Super-resolution and light-sheet microscopes that can visualize subcellular structures at a higher spatial resolution, record long term cell behavior, and track cells in intact animals, and
    (2) New fluorescent probes based on fluorescent proteins, nanobodies and aptamers so that biological questions can be converted into "imageable" ones.

    Collapse Research 
    Collapse Research Activities and Funding
    Modulation and functional characterization of protein condensation in chromatin organization
    NIH U01DK127421Sep 15, 2020 - Jun 30, 2025
    Role: Principal Investigator
    Mapping endogenous protein dynamics in living cells
    NIH R01GM131641Sep 20, 2019 - Aug 31, 2023
    Role: Principal Investigator
    (PQ7) Defining a new mode of RAS signaling in cancer from cytoplasmic protein granules
    NIH R01CA231300Jul 1, 2019 - Jun 30, 2024
    Role: Co-Principal Investigator
    Rapid screening of gene-edited cells
    NIH R21GM129652Aug 1, 2018 - Jun 30, 2020
    Role: Principal Investigator
    Structure mapping of molecular complexes by super-resolution microscopy
    NIH R01GM124334May 4, 2018 - Mar 31, 2022
    Role: Principal Investigator
    Multimerized GFP probe for live cell imaging
    NIH R21EB022798Jun 1, 2016 - Mar 31, 2018
    Role: Principal Investigator
    Computational design of Cas9-based molecular imaging reagents
    NIH R21EB021453May 10, 2016 - Feb 28, 2018
    Role: Co-Principal Investigator
    Analyzing genomic elements in live animals by CRISPR imaging
    NIH R33EB019784Sep 25, 2014 - Jun 30, 2017
    Role: Principal Investigator
    The Role of Dscam Dock and Cdc42 Effectors in Prosophilia Neuron Dendritogenesis
    NIH R21MH101688Mar 15, 2014 - Feb 29, 2016
    Role: Principal Investigator
    Advancing Whole-Organism Screening for Chemical Modifiers of the Brain
    NIH R21NS082938Feb 1, 2013 - Jan 31, 2016
    Role: Co-Principal Investigator
    Solving macromolecular complex architecture in situ by super-resolution microscop
    NIH DP2OD008479Sep 30, 2011 - Jun 30, 2016
    Role: Principal Investigator

    Collapse ORNG Applications 
    Collapse Academic Senate

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help. to make corrections and additions.
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    Altmetrics Details PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. HybriSeq: Probe-based Device-free Single-cell RNA Profiling. bioRxiv. 2023 Sep 29. Foyt D, Brown D, Zhou S, Huang B. PMID: 37808850; PMCID: PMC10557710.
      View in: PubMed   Mentions:
    2. Efficient tagging of endogenous proteins in human cell lines for structural studies by single-particle cryo-EM. Proc Natl Acad Sci U S A. 2023 08; 120(31):e2302471120. Choi W, Wu H, Yserentant K, Huang B, Cheng Y. PMID: 37487103; PMCID: PMC10401002.
      View in: PubMed   Mentions:    Fields:    Translation:HumansCells
    3. Spatial and temporal organization of the genome: Current state and future aims of the 4D nucleome project. Mol Cell. 2023 08 03; 83(15):2624-2640. Dekker J, Alber F, Aufmkolk S, Beliveau BJ, Bruneau BG, Belmont AS, Bintu L, Boettiger A, Calandrelli R, Disteche CM, Gilbert DM, Gregor T, Hansen AS, Huang B, Huangfu D, Kalhor R, Leslie CS, Li W, Li Y, Ma J, Noble WS, Park PJ, Phillips-Cremins JE, Pollard KS, Rafelski SM, Ren B, Ruan Y, Shav-Tal Y, Shen Y, Shendure J, Shu X, Strambio-De-Castillia C, Vertii A, Zhang H, Zhong S. PMID: 37419111; PMCID: PMC10528254.
      View in: PubMed   Mentions: 5     Fields:    Translation:Cells
    4. CELL-E: A Text-To-Image Transformer for Protein Localization Prediction. Res Sq. 2023 Jun 02. Khwaja E, Song YS, Huang B. PMID: 37398207; PMCID: PMC10312902.
      View in: PubMed   Mentions:
    5. Structural insights into the human PA28-20S proteasome enabled by efficient tagging and purification of endogenous proteins. Proc Natl Acad Sci U S A. 2022 08 16; 119(33):e2207200119. Zhao J, Makhija S, Zhou C, Zhang H, Wang Y, Muralidharan M, Huang B, Cheng Y. PMID: 35858375; PMCID: PMC9388094.
      View in: PubMed   Mentions: 5     Fields:    Translation:HumansCells
    6. High activity and high functional connectivity are mutually exclusive in resting state zebrafish and human brains. BMC Biol. 2022 04 11; 20(1):84. Zarei M, Xie D, Jiang F, Bagirov A, Huang B, Raj A, Nagarajan S, Guo S. PMID: 35410342; PMCID: PMC8996543.
      View in: PubMed   Mentions: 1     Fields:    Translation:HumansAnimalsCells
    7. In Vivo Dopamine Neuron Imaging-Based Small Molecule Screen Identifies Novel Neuroprotective Compounds and Targets. Front Pharmacol. 2022; 13:837756. Kim GJ, Mo H, Liu H, Okorie M, Chen S, Zheng J, Li H, Arkin M, Huang B, Guo S. PMID: 35370735; PMCID: PMC8971663.
      View in: PubMed   Mentions: 3  
    8. OpenCell: Endogenous tagging for the cartography of human cellular organization. Science. 2022 03 11; 375(6585):eabi6983. Cho NH, Cheveralls KC, Brunner AD, Kim K, Michaelis AC, Raghavan P, Kobayashi H, Savy L, Li JY, Canaj H, Kim JYS, Stewart EM, Gnann C, McCarthy F, Cabrera JP, Brunetti RM, Chhun BB, Dingle G, Hein MY, Huang B, Mehta SB, Weissman JS, Gómez-Sjöberg R, Itzhak DN, Royer LA, Mann M, Leonetti MD. PMID: 35271311; PMCID: PMC9119736.
      View in: PubMed   Mentions: 92     Fields:    Translation:HumansCells
    9. Image denoising for fluorescence microscopy by supervised to self-supervised transfer learning. Optics Express. 2021; 29:41303-41312. Y. Wang, H. Pinkard, E. Khwaja, S. Zhou, L. Waller, B. Huang. .
    10. A zebrafish screen reveals Renin-angiotensin system inhibitors as neuroprotective via mitochondrial restoration in dopamine neurons. Elife. 2021 09 22; 10. Kim GJ, Mo H, Liu H, Wu Z, Chen S, Zheng J, Zhao X, Nucum D, Shortland J, Peng L, Elepano M, Tang B, Olson S, Paras N, Li H, Renslo AR, Arkin MR, Huang B, Lu B, Sirota M, Guo S. PMID: 34550070; PMCID: PMC8457844.
      View in: PubMed   Mentions: 8     Fields:    Translation:HumansAnimalsCells
    11. A ciliopathy complex builds distal appendages to initiate ciliogenesis. J Cell Biol. 2021 09 06; 220(9). Kumar D, Rains A, Herranz-Pérez V, Lu Q, Shi X, Swaney DL, Stevenson E, Krogan NJ, Huang B, Westlake C, Garcia-Verdugo JM, Yoder BK, Reiter JF. PMID: 34241634; PMCID: PMC8276316.
      View in: PubMed   Mentions: 17     Fields:    Translation:HumansAnimalsCells
    12. Label-retention expansion microscopy. J Cell Biol. 2021 09 06; 220(9). Shi X, Li Q, Dai Z, Tran AA, Feng S, Ramirez AD, Lin Z, Wang X, Chow TT, Chen J, Kumar D, McColloch AR, Reiter JF, Huang EJ, Seiple IB, Huang B. PMID: 34228783; PMCID: PMC8266563.
      View in: PubMed   Mentions: 19     Fields:    Translation:HumansAnimalsCells
    13. Polarized endosome dynamics engage cytoplasmic Par-3 that recruits dynein during asymmetric cell division. Sci Adv. 2021 06; 7(24). Zhao X, Garcia JQ, Tong K, Chen X, Yang B, Li Q, Dai Z, Shi X, Seiple IB, Huang B, Guo S. PMID: 34117063; PMCID: PMC8195473.
      View in: PubMed   Mentions: 6     Fields:    Translation:AnimalsCells
    14. Kinase-mediated RAS signaling via membraneless cytoplasmic protein granules. Cell. 2021 05 13; 184(10):2649-2664.e18. Tulpule A, Guan J, Neel DS, Allegakoen HR, Lin YP, Brown D, Chou YT, Heslin A, Chatterjee N, Perati S, Menon S, Nguyen TA, Debnath J, Ramirez AD, Shi X, Yang B, Feng S, Makhija S, Huang B, Bivona TG. PMID: 33848463; PMCID: PMC8127962.
      View in: PubMed   Mentions: 63     Fields:    Translation:HumansCells
    15. Versatile Labeling and Detection of Endogenous Proteins Using Tag-Assisted Split Enzyme Complementation. ACS Chem Biol. 2021 04 16; 16(4):671-681. Makhija S, Brown D, Rudlaff RM, Doh JK, Bourke S, Wang Y, Zhou S, Cheloor-Kovilakam R, Huang B. PMID: 33734687; PMCID: PMC8115985.
      View in: PubMed   Mentions: 7     Fields:    Translation:HumansCells
    16. HP1 proteins compact DNA into mechanically and positionally stable phase separated domains. Elife. 2021 03 04; 10. Keenen MM, Brown D, Brennan LD, Renger R, Khoo H, Carlson CR, Huang B, Grill SW, Narlikar GJ, Redding S. PMID: 33661100; PMCID: PMC7932698.
      View in: PubMed   Mentions: 63     Fields:    Translation:HumansCells
    17. Spatial decoding of endosomal cAMP signals by a metastable cytoplasmic PKA network. Nat Chem Biol. 2021 05; 17(5):558-566. Peng GE, Pessino V, Huang B, von Zastrow M. PMID: 33649598; PMCID: PMC8084946.
      View in: PubMed   Mentions: 11     Fields:    Translation:HumansAnimalsCells
    18. Improved yellow-green split fluorescent proteins for protein labeling and signal amplification. PLoS One. 2020; 15(11):e0242592. Zhou S, Feng S, Brown D, Huang B. PMID: 33227014; PMCID: PMC7682878.
      View in: PubMed   Mentions: 2     Fields:    Translation:HumansCells
    19. Nanotopography Enhances Dynamic Remodeling of Tight Junction Proteins through Cytosolic Liquid Complexes. ACS Nano. 2020 10 27; 14(10):13192-13202. Huang X, Shi X, Hansen ME, Setiady I, Nemeth CL, Celli A, Huang B, Mauro T, Koval M, Desai TA. PMID: 32940450; PMCID: PMC7606830.
      View in: PubMed   Mentions: 6     Fields:    Translation:Cells
    20. Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency. Nature. 2020 12; 588(7838):459-465. Zhang J, Velmeshev D, Hashimoto K, Huang YH, Hofmann JW, Shi X, Chen J, Leidal AM, Dishart JG, Cahill MK, Kelley KW, Liddelow SA, Seeley WW, Miller BL, Walther TC, Farese RV, Taylor JP, Ullian EM, Huang B, Debnath J, Wittmann T, Kriegstein AR, Huang EJ. PMID: 32866962; PMCID: PMC7746606.
      View in: PubMed   Mentions: 64     Fields:    Translation:AnimalsCells
    21. A molecular mechanism for probabilistic bet hedging and its role in viral latency. Proc Natl Acad Sci U S A. 2020 07 21; 117(29):17240-17248. Chaturvedi S, Klein J, Vardi N, Bolovan-Fritts C, Wolf M, Du K, Mlera L, Calvert M, Moorman NJ, Goodrum F, Huang B, Weinberger LS. PMID: 32632017; PMCID: PMC7382263.
      View in: PubMed   Mentions: 8     Fields:    Translation:HumansCells
    22. Bright split red fluorescent proteins for the visualization of endogenous proteins and synapses. Commun Biol. 2019; 2:344. Feng S, Varshney A, Coto Villa D, Modavi C, Kohler J, Farah F, Zhou S, Ali N, Müller JD, Van Hoven MK, Huang B. PMID: 31552297; PMCID: PMC6749000.
      View in: PubMed   Mentions: 19  Translation:HumansCells
    23. Live-cell imaging reveals enhancer-dependent Sox2 transcription in the absence of enhancer proximity. Elife. 2019 05 24; 8. Alexander JM, Guan J, Li B, Maliskova L, Song M, Shen Y, Huang B, Lomvardas S, Weiner OD. PMID: 31124784; PMCID: PMC6534382.
      View in: PubMed   Mentions: 128     Fields:    Translation:AnimalsCells
    24. Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution. Nat Methods. 2019 06; 16(6):501-504. Yang B, Chen X, Wang Y, Feng S, Pessino V, Stuurman N, Cho NH, Cheng KW, Lord SJ, Xu L, Xie D, Mullins RD, Leonetti MD, Huang B. PMID: 31061492; PMCID: PMC6557432.
      View in: PubMed   Mentions: 54     Fields:    Translation:HumansAnimalsCells
    25. The Extracellular RNA Communication Consortium: Establishing Foundational Knowledge and Technologies for Extracellular RNA Research. Cell. 2019 04 04; 177(2):231-242. Das S, Extracellular RNA Communication Consortium, Ansel KM, Bitzer M, Breakefield XO, Charest A, Galas DJ, Gerstein MB, Gupta M, Milosavljevic A, McManus MT, Patel T, Raffai RL, Rozowsky J, Roth ME, Saugstad JA, Van Keuren-Jensen K, Weaver AM, Laurent LC. PMID: 30951667; PMCID: PMC6601620.
      View in: PubMed   Mentions: 97     Fields:    Translation:HumansCells
    26. Deformed alignment of super-resolution images for semi-flexible structures. PLoS One. 2019; 14(3):e0212735. Shi X, Garcia G, Wang Y, Reiter JF, Huang B. PMID: 30865666; PMCID: PMC6415779.
      View in: PubMed   Mentions: 7     Fields:    
    27. Multicolor fluorescent imaging by space-constrained computational spectral imaging. Opt Express. 2019 Feb 18; 27(4):5393-5402. Wang Y, Yang B, Feng S, Pessino V, Huang B. PMID: 30876144; PMCID: PMC6410920.
      View in: PubMed   Mentions: 3     Fields:    
    28. Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag. Nat Commun. 2018 11 29; 9(1):5065. Chen B, Zou W, Xu H, Liang Y, Huang B. PMID: 30498221; PMCID: PMC6265289.
      View in: PubMed   Mentions: 39     Fields:    Translation:HumansCells
    29. Local enrichment of HP1alpha at telomeres alters their structure and regulation of telomere protection. Nat Commun. 2018 09 04; 9(1):3583. Chow TT, Shi X, Wei JH, Guan J, Stadler G, Huang B, Blackburn EH. PMID: 30181605; PMCID: PMC6123478.
      View in: PubMed   Mentions: 12     Fields:    Translation:HumansCells
    30. A LINE1-Nucleolin Partnership Regulates Early Development and ESC Identity. Cell. 2018 07 12; 174(2):391-405.e19. Percharde M, Lin CJ, Yin Y, Guan J, Peixoto GA, Bulut-Karslioglu A, Biechele S, Huang B, Shen X, Ramalho-Santos M. PMID: 29937225; PMCID: PMC6046266.
      View in: PubMed   Mentions: 217     Fields:    Translation:AnimalsCells
    31. Autoinhibition of kinesin-1 is essential to the dendrite-specific localization of Golgi outposts. J Cell Biol. 2018 07 02; 217(7):2531-2547. Kelliher MT, Yue Y, Ng A, Kamiyama D, Huang B, Verhey KJ, Wildonger J. PMID: 29728423; PMCID: PMC6028532.
      View in: PubMed   Mentions: 31     Fields:    Translation:AnimalsCells
    32. Co-translational protein targeting facilitates centrosomal recruitment of PCNT during centrosome maturation in vertebrates. Elife. 2018 04 30; 7. Sepulveda G, Antkowiak M, Brust-Mascher I, Mahe K, Ou T, Castro NM, Christensen LN, Cheung L, Jiang X, Yoon D, Huang B, Jao LE. PMID: 29708497; PMCID: PMC5976437.
      View in: PubMed   Mentions: 47     Fields:    Translation:HumansAnimalsCells
    33. Correlation analysis framework for localization-based superresolution microscopy. Proc Natl Acad Sci U S A. 2018 03 27; 115(13):3219-3224. Schnitzbauer J, Wang Y, Zhao S, Bakalar M, Nuwal T, Chen B, Huang B. PMID: 29531072; PMCID: PMC5879654.
      View in: PubMed   Mentions: 16     Fields:    Translation:AnimalsCells
    34. 2-Hydroxyglutarate-Mediated Autophagy of the Endoplasmic Reticulum Leads to an Unusual Downregulation of Phospholipid Biosynthesis in Mutant IDH1 Gliomas. Cancer Res. 2018 05 01; 78(9):2290-2304. Viswanath P, Radoul M, Izquierdo-Garcia JL, Ong WQ, Luchman HA, Cairncross JG, Huang B, Pieper RO, Phillips JJ, Ronen SM. PMID: 29358170; PMCID: PMC5932252.
      View in: PubMed   Mentions: 29     Fields:    Translation:HumansAnimalsCells
    35. The centrosomin CM2 domain is a multi-functional binding domain with distinct cell cycle roles. PLoS One. 2018; 13(1):e0190530. Citron YR, Fagerstrom CJ, Keszthelyi B, Huang B, Rusan NM, Kelly MJS, Agard DA. PMID: 29315319; PMCID: PMC5760045.
      View in: PubMed   Mentions: 9     Fields:    Translation:AnimalsCells
    36. Improved split fluorescent proteins for endogenous protein labeling. Nat Commun. 2017 08 29; 8(1):370. Feng S, Sekine S, Pessino V, Li H, Leonetti MD, Huang B. PMID: 28851864; PMCID: PMC5575300.
      View in: PubMed   Mentions: 103     Fields:    Translation:HumansCells
    37. Super-resolution microscopy reveals that disruption of ciliary transition-zone architecture causes Joubert syndrome. Nat Cell Biol. 2017 Oct; 19(10):1178-1188. Shi X, Garcia G, Van De Weghe JC, McGorty R, Pazour GJ, Doherty D, Huang B, Reiter JF. PMID: 28846093; PMCID: PMC5695680.
      View in: PubMed   Mentions: 77     Fields:    Translation:HumansAnimalsCells
    38. High-NA open-top selective-plane illumination microscopy for biological imaging. Opt Express. 2017 Jul 24; 25(15):17798-17810. Mcgorty R, Xie D, Huang B. PMID: 28789271; PMCID: PMC5557333.
      View in: PubMed   Mentions: 22     Fields:    
    39. Covalent Protein Labeling by SpyTag-SpyCatcher in Fixed Cells for Super-Resolution Microscopy. Chembiochem. 2017 08 04; 18(15):1492-1495. Pessino V, Citron YR, Feng S, Huang B. PMID: 28514494; PMCID: PMC5599254.
      View in: PubMed   Mentions: 15     Fields:    Translation:HumansCells
    40. Functional selectivity of GPCR-directed drug action through location bias. Nat Chem Biol. 2017 Jul; 13(7):799-806. Irannejad R, Pessino V, Mika D, Huang B, Wedegaertner PB, Conti M, von Zastrow M. PMID: 28553949; PMCID: PMC5733145.
      View in: PubMed   Mentions: 93     Fields:    Translation:HumansCells
    41. Tracking Multiple Genomic Elements Using Correlative CRISPR Imaging and Sequential DNA FISH. Biophys J. 2017 Mar 28; 112(6):1077-1084. Guan J, Liu H, Shi X, Feng S, Huang B. PMID: 28355536; PMCID: PMC5375138.
      View in: PubMed   Mentions: 20     Fields:    Translation:HumansCells
    42. EGF and NRG induce phosphorylation of HER3/ERBB3 by EGFR using distinct oligomeric mechanisms. Proc Natl Acad Sci U S A. 2017 04 04; 114(14):E2836-E2845. van Lengerich B, Agnew C, Puchner EM, Huang B, Jura N. PMID: 28320942; PMCID: PMC5389333.
      View in: PubMed   Mentions: 43     Fields:    Translation:HumansAnimalsCells
    43. Live Cell Imaging of Endogenous mRNA Using RNA-Based Fluorescence "Turn-On" Probe. ACS Chem Biol. 2017 01 20; 12(1):200-205. Ong WQ, Citron YR, Sekine S, Huang B. PMID: 28103687; PMCID: PMC5460623.
      View in: PubMed   Mentions: 12     Fields:    Translation:Cells
    44. A microscopic view of the cell. Mol Biol Cell. 2016 11 01; 27(21):3183-3184. Huang B. PMID: 27799489; PMCID: PMC5170846.
      View in: PubMed   Mentions:    Fields:    
    45. A High-Content Larval Zebrafish Brain Imaging Method for Small Molecule Drug Discovery. PLoS One. 2016; 11(10):e0164645. Liu H, Chen S, Huang K, Kim J, Mo H, Iovine R, Gendre J, Pascal P, Li Q, Sun Y, Dong Z, Arkin M, Guo S, Huang B. PMID: 27732643; PMCID: PMC5061318.
      View in: PubMed   Mentions: 7     Fields:    Translation:AnimalsCells
    46. Identification of environmental stressors and validation of light preference as a measure of anxiety in larval zebrafish. BMC Neurosci. 2016 09 15; 17(1):63. Bai Y, Liu H, Huang B, Wagle M, Guo S. PMID: 27633776; PMCID: PMC5025543.
      View in: PubMed   Mentions: 26     Fields:    Translation:Animals
    47. A scalable strategy for high-throughput GFP tagging of endogenous human proteins. Proc Natl Acad Sci U S A. 2016 06 21; 113(25):E3501-8. Leonetti MD, Sekine S, Kamiyama D, Weissman JS, Huang B. PMID: 27274053; PMCID: PMC4922190.
      View in: PubMed   Mentions: 112     Fields:    Translation:HumansCells
    48. Imaging Specific Genomic DNA in Living Cells. Annu Rev Biophys. 2016 07 05; 45:1-23. Chen B, Guan J, Huang B. PMID: 27145877; PMCID: PMC5053920.
      View in: PubMed   Mentions: 39     Fields:    Translation:HumansAnimalsCells
    49. Versatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016 Mar 18; 7:11046. Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B. PMID: 26988139; PMCID: PMC4802074.
      View in: PubMed   Mentions: 180     Fields:    Translation:HumansCells
    50. Force Feedback Controls Motor Activity and Mechanical Properties of Self-Assembling Branched Actin Networks. Cell. 2016 Jan 14; 164(1-2):115-127. Bieling P, Li TD, Weichsel J, McGorty R, Jreij P, Huang B, Fletcher DA, Mullins RD. PMID: 26771487; PMCID: PMC5033619.
      View in: PubMed   Mentions: 121     Fields:    Translation:Humans
    51. Expanding the CRISPR imaging toolset with Staphylococcus aureus Cas9 for simultaneous imaging of multiple genomic loci. Nucleic Acids Res. 2016 05 05; 44(8):e75. Chen B, Hu J, Almeida R, Liu H, Balakrishnan S, Covill-Cooke C, Lim WA, Huang B. PMID: 26740581; PMCID: PMC4856973.
      View in: PubMed   Mentions: 76     Fields:    Translation:HumansAnimalsCells
    52. Specification of Dendritogenesis Site in Drosophila aCC Motoneuron by Membrane Enrichment of Pak1 through Dscam1. Dev Cell. 2015 Oct 12; 35(1):93-106. Kamiyama D, McGorty R, Kamiyama R, Kim MD, Chiba A, Huang B. PMID: 26460947; PMCID: PMC4626010.
      View in: PubMed   Mentions: 21     Fields:    Translation:AnimalsCells
    53. Heavy water: a simple solution to increasing the brightness of fluorescent proteins in super-resolution imaging. Chem Commun (Camb). 2015 Sep 11; 51(70):13451-3. Ong WQ, Citron YR, Schnitzbauer J, Kamiyama D, Huang B. PMID: 26226621; PMCID: PMC4544551.
      View in: PubMed   Mentions: 7     Fields:    
    54. Biogenesis, delivery, and function of extracellular RNA. J Extracell Vesicles. 2015; 4:27494. Patton JG, Franklin JL, Weaver AM, Vickers K, Zhang B, Coffey RJ, Ansel KM, Blelloch R, Goga A, Huang B, L'Etoille N, Raffai RL, Lai CP, Krichevsky AM, Mateescu B, Greiner VJ, Hunter C, Voinnet O, McManus MT. PMID: 26320939; PMCID: PMC4553266.
      View in: PubMed   Mentions: 46     Fields:    
    55. Open-top selective plane illumination microscope for conventionally mounted specimens. Opt Express. 2015 Jun 15; 23(12):16142-53. McGorty R, Liu H, Kamiyama D, Dong Z, Guo S, Huang B. PMID: 26193587; PMCID: PMC4523553.
      View in: PubMed   Mentions: 42     Fields:    
    56. Single molecule techniques--applications in biology. FEBS Lett. 2014 Oct 01; 588(19):3519. Puchner EM, Huang B, Gaub HE. PMID: 25157433.
      View in: PubMed   Mentions: 1     Fields:    
    57. Localization events-based sample drift correction for localization microscopy with redundant cross-correlation algorithm. Opt Express. 2014 Jun 30; 22(13):15982-91. Wang Y, Schnitzbauer J, Hu Z, Li X, Cheng Y, Huang ZL, Huang B. PMID: 24977854; PMCID: PMC4162368.
      View in: PubMed   Mentions: 66     Fields:    Translation:Animals
    58. Surface-chemistry effect on cellular response of luminescent plasmonic silver nanoparticles. Bioconjug Chem. 2014 Mar 19; 25(3):453-9. Sun S, Zhou C, Chen S, Liu J, Yu J, Chilek J, Zhao L, Yu M, Vinluan R, Huang B, Zheng J. PMID: 24559325; PMCID: PMC3983130.
      View in: PubMed   Mentions: 2     Fields:    Translation:HumansCells
    59. Correction of depth-dependent aberrations in 3D single-molecule localization and super-resolution microscopy. Opt Lett. 2014 Jan 15; 39(2):275-8. McGorty R, Schnitzbauer J, Zhang W, Huang B. PMID: 24562125; PMCID: PMC4030053.
      View in: PubMed   Mentions: 25     Fields:    Translation:AnimalsCells
    60. Imaging genomic elements in living cells using CRISPR/Cas9. Methods Enzymol. 2014; 546:337-54. Chen B, Huang B. PMID: 25398348.
      View in: PubMed   Mentions: 18     Fields:    Translation:HumansCells
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    63. Counting molecules in single organelles with superresolution microscopy allows tracking of the endosome maturation trajectory. Proc Natl Acad Sci U S A. 2013 Oct 01; 110(40):16015-20. Puchner EM, Walter JM, Kasper R, Huang B, Lim WA. PMID: 24043832; PMCID: PMC3791776.
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    69. Development in the STORM. Dev Cell. 2012 Dec 11; 23(6):1103-10. Kamiyama D, Huang B. PMID: 23237944; PMCID: PMC3523271.
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    73. Afadin, a Ras/Rap effector that controls cadherin function, promotes spine and excitatory synapse density in the hippocampus. J Neurosci. 2012 Jan 04; 32(1):99-110. Beaudoin GM, Schofield CM, Nuwal T, Zang K, Ullian EM, Huang B, Reichardt LF. PMID: 22219273; PMCID: PMC3305287.
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    74. SnapShot: Light microscopy. Cell. 2011 Nov 23; 147(5):1198.e1. Kasper R, Huang B. PMID: 22118471.
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    78. Coupling between clathrin-dependent endocytic budding and F-BAR-dependent tubulation in a cell-free system. Nat Cell Biol. 2010 Sep; 12(9):902-8. Wu M, Huang B, Graham M, Raimondi A, Heuser JE, Zhuang X, De Camilli P. PMID: 20729836; PMCID: PMC3338250.
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    79. New resolving power for light microscopy: applications to neurobiology. Curr Opin Neurobiol. 2010 Oct; 20(5):648-52. Dani A, Huang B. PMID: 20728340.
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    80. Super-resolution optical microscopy: multiple choices. Curr Opin Chem Biol. 2010 Feb; 14(1):10-4. Huang B. PMID: 19897404.
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    82. Whole-cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution. Nat Methods. 2008 Dec; 5(12):1047-52. Huang B, Jones SA, Brandenburg B, Zhuang X. PMID: 19029906; PMCID: PMC2596623.
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    83. Super-resolution microscopy by nanoscale localization of photo-switchable fluorescent probes. Curr Opin Chem Biol. 2008 Oct; 12(5):505-14. Bates M, Huang B, Zhuang X. PMID: 18809508; PMCID: PMC2642911.
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    84. Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy. Science. 2008 Feb 08; 319(5864):810-3. Huang B, Wang W, Bates M, Zhuang X. PMID: 18174397; PMCID: PMC2633023.
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    85. Microfluidic separation and capture of analytes for single-molecule spectroscopy. Lab Chip. 2007 Dec; 7(12):1663-5. Kim S, Huang B, Zare RN. PMID: 18030384.
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    88. A monomeric G protein-coupled receptor isolated in a high-density lipoprotein particle efficiently activates its G protein. Proc Natl Acad Sci U S A. 2007 May 01; 104(18):7682-7. Whorton MR, Bokoch MP, Rasmussen SG, Huang B, Zare RN, Kobilka B, Sunahara RK. PMID: 17452637; PMCID: PMC1863461.
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    89. Surface plasmon resonance imaging using a high numerical aperture microscope objective. Anal Chem. 2007 Apr 01; 79(7):2979-83. Huang B, Yu F, Zare RN. PMID: 17309232.
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    91. Controlling electroosmotic flow in poly(dimethylsiloxane) separation channels by means of prepolymer additives. Anal Chem. 2006 Jul 01; 78(13):4588-92. Luo Y, Huang B, Wu H, Zare RN. PMID: 16808469.
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    92. Generation of complex, static solution gradients in microfluidic channels. J Am Chem Soc. 2006 Apr 05; 128(13):4194-5. Wu H, Huang B, Zare RN. PMID: 16568971.
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    94. Construction of microfluidic chips using polydimethylsiloxane for adhesive bonding. Lab Chip. 2005 Dec; 5(12):1393-8. Wu H, Huang B, Zare RN. PMID: 16286971.
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    96. Effect of bin time on the photon counting histogram for one-photon excitation. Chemphyschem. 2005 May; 6(5):905-12. Perroud TD, Huang B, Zare RN. PMID: 15884075.
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    97. Photon counting histogram: one-photon excitation. Chemphyschem. 2004 Oct 18; 5(10):1523-31. Huang B, Perroud TD, Zare RN. PMID: 15535551.
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    100. Fluorescence emission and absorption spectra of single Anabaena sp. strain PCC7120 cells. Photochem Photobiol. 2002 Sep; 76(3):310-3. Ying L, Huang X, Huang B, Xie J, Zhao J, Zhao XS. PMID: 12403452.
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