Joanne Engel, MD, PhD

Title(s)Professor, Medicine
SchoolSchool of Medicine
Address513 Parnassus Ave, HSE, #405
San Francisco CA 94143
Pronounsshe/her/hers
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    Collapse Biography 
    Collapse Education and Training
    University of California, San Francisco, San FranciscoClinical and postdoctoral fellowship1990Infectious Disease
    University of Pennsylvania, Philadelphia, PAResidency 1986Internal Medicine
    Stanford University, Stanford, CAM.D., PH.D.1983School of Medicine

    Collapse Overview 
    Collapse Overview
    My lab is interested in the complex interplay between bacterial pathogens and host cells. In particular, we study two important human pathogens, Chlamydia trachomatis and Pseudomonas aeruginosa. Our strengths include using multidisciplinary approaches to these studies—allowing the pathogen to be our tutor. We have utilized bacterial genetics and genetic screens, molecular biology, cellular microbiology, host cell biology with advanced immunofluorescence microscopy, genome-wide RNAi screens, bioinformatics, and proteomics to rigorously understand the mechanisms by which they subvert host cell functions to cause disease. Seminal contributions that our group has made to the study of P. aeruginosa-host interactions is (i) the discovery of the P. aeruginosa type III secretion system and one of the secreted effectors ExoU and the demonstration that the P. aeruginosa type III secretion system is important for virulence in cell-culture, mouse, and human infections (ii) demonstrating that the type III secreted toxin ExoT inhibits wound repair through redundant pathways (iii) elucidation of the pathway by which P. aeruginosa can be internalized by non-phagocytic cells and how the type III secretion system-encoded effectors modulate entry (iv) characterization of novel genes involved in type IV pilin biogenesis and in the regulation of diverse virulence pathways (v) the first identification of a host cell ubiquitin ligase (cbl-b) that specifically targets the degradation of a type III secreted factor (vii) development of 2D and 3D cell-culture based systems to dissect the interaction of pathogens with the apical versus basolateral surface of polarized epithelial cells (vi) discovery that upon binding to the apical surface of polarized epithelial cells, P. aeruginosa forms biofilm-like structures that are able to transform apical membrane into basolateral membrane by exploiting the phosphatidyl inositol kinase pathway to form membrane protrusions that are associated with a spatial and temporal activation of the innate immune response. Our current studies focus on the dissection of the Chp/Vfr/ regulatory pathway that regulates diverse virulence factor circuits in P. aeruginosa in determining the bacterial and host determinants involved in the formation of biofilms and spatially localized activation of the innate immune response at the apical surface of tissues. In our studies on the pathogenesis of chlamydial infections, we have focused on host cell biology and genome-wide RNA-based screens to understanding how C. trachomatis modulates host cell signaling systems to bind, enter, and establish a replicative niche. We have carried out a genome wide RNAi screen in a simple genetic host and have identified new host molecules that are involved in binding, entry, and establishment of a unique intracellular niche. We have discovered a potential role for host growth factors in binding and entry and elucidated a novel pathway by which this organism acquires sphingolipids from the host. We have complemented these studies with state of the art confocal microscopy to begin to elucidate the bacterial and host determinants and mechanism of vacuole fusion. We are currently carrying out high throughput proteomics to dissect the function of the approximately 150 proteins that Chlamydia inject into the host cell to create a unique replicative niche and to escape the innate immune response.

    Collapse Research 
    Collapse Research Activities and Funding
    Inclusion membrane protein (Inc) modulation of the innate immune response to Chlamydia trachomatis
    NIH R56AI152526Sep 4, 2020 - Aug 31, 2021
    Role: Principal Investigator
    Understanding the role of sensory adaptation in bacterial mechanochemical signaling pathways
    NIH R21AI154350Jul 1, 2020 - Jun 30, 2022
    Role: Principal Investigator
    Adapting to a changing environment: How surface contact induces virulence factor production in Pseudomonas aeruginosa
    NIH R01AI129547Aug 1, 2017 - Jul 31, 2021
    Role: Principal Investigator
    Decoding the Chlamydia inclusion membrane protein-host protein interactome
    NIH R01AI122747Dec 1, 2015 - Nov 30, 2020
    Role: Principal Investigator
    High throughput proteomics to dissect Chlamydia-host cell interactions
    NIH R21AI105561Sep 17, 2013 - Aug 31, 2015
    Role: Principal Investigator
    Proteomic approach to identify mediators of PI3K activation by P. aeruginosa
    NIH R03TW008056Apr 1, 2008 - Mar 31, 2012
    Role: Principal Investigator
    Novel approaches to identify host genes required for Chlamydia pathogenesis
    NIH R01AI073770Apr 15, 2007 - Aug 31, 2018
    Role: Principal Investigator
    New approaches to study Pseudomonas-host interactions
    NIH R56AI065902May 1, 2005 - Jul 31, 2017
    Role: Principal Investigator
    New approaches to study Pseudomonas-host interactions
    NIH R01AI065902May 1, 2005 - May 31, 2015
    Role: Principal Investigator
    New approaches to studying host-chlamydia interactions
    NIH R21AI062768Apr 1, 2005 - Mar 31, 2007
    Role: Principal Investigator
    Microbial Pathogenesis and Host Defense
    NIH T32AI060537Aug 1, 2004 - Jul 31, 2024
    Role: Principal Investigator
    Mucosal Immune Barrier in Infection and Immunity
    NIH P01AI053194Sep 30, 2002 - Aug 31, 2014
    Role: Co-Investigator
    BIOGENESIS OF THE CHLAMYDIA TRACHOMATIS VACUOLE
    NIH R01AI044148Feb 1, 2000 - Jan 31, 2006
    Role: Principal Investigator
    Mechanism of Pseudomonas-mediated epithelial cell damage
    NIH R01AI042806Jun 1, 1998 - Apr 30, 2012
    Role: Principal Investigator
    Comprehensive Biology: Exploiting the Yeast Genome
    NIH P41RR011823Sep 30, 1996 - Jun 30, 2016
    Role: Co-Investigator
    CELL BIOLOGY OF CHLAMYDIA, TRACHOMATIS
    NIH R21AI038271Sep 1, 1996 - Oct 31, 1997
    Role: Principal Investigator
    MOLECULAR AND CELL BIOLOGY OF CHLAMYDIA TRACHOMATIS
    NIH K04AI001348Jun 1, 1996 - May 31, 2001
    Role: Principal Investigator
    MOLECULAR BIOLOGY OF CHLAMYDIAL DEVELOPMENT
    NIH R01AI024436Feb 1, 1988 - Jan 31, 2002
    Role: Principal Investigator

    Collapse ORNG Applications 
    Collapse Collaboration Interests
    Collapse Academic Senate

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help. to make corrections and additions.
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    Altmetrics Details PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Spatial control of sensory adaptation modulates mechanosensing in Pseudomonas aeruginosa. bioRxiv. 2024 Feb 27. Patino R, Kühn MJ, Macmillan H, Inclan YF, Chavez I, Von Dollen J, Johnson JR, Swaney DL, Krogan NJ, Persat A, Engel JN. PMID: 38464290; PMCID: PMC10925122.
      View in: PubMed   Mentions:
    2. Peptidoglycan-Targeted [18F]3,3,3-Trifluoro-d-alanine Tracer for Imaging Bacterial Infection. JACS Au. 2024 Feb 25. Sorlin SA, Lo´pez-A´lvarez LM, Biboy BJ, Gray GJ, Rabbitt RS, Rahim RJ, Lee LS, Bobba BK, Blecha BJ, Parker PM, Flavell FR, Engel EJ, Ohliger OM, Vollmer VW, Wilson WD. .
      View in: Publisher Site   Mentions:
    3. Two antagonistic response regulators control Pseudomonas aeruginosa polarization during mechanotaxis. EMBO J. 2023 04 03; 42(7):e112165. Kühn MJ, Macmillan H, Talà L, Inclan Y, Patino R, Pierrat X, Al-Mayyah Z, Engel JN, Persat A. PMID: 36795017; PMCID: PMC10519157.
      View in: PubMed   Mentions: 3     Fields:    Translation:Cells
    4. Imaging joint infections using D-methyl-11C-methionine PET/MRI: initial experience in humans. Eur J Nucl Med Mol Imaging. 2022 09; 49(11):3761-3771. Polvoy I, Seo Y, Parker M, Stewart M, Siddiqua K, Manacsa HS, Ravanfar V, Blecha J, Hope TA, Vanbrocklin H, Flavell RR, Barry J, Hansen E, Villanueva-Meyer JE, Engel J, Rosenberg OS, Wilson DM, Ohliger MA. PMID: 35732972; PMCID: PMC9399217.
      View in: PubMed   Mentions: 8     Fields:    Translation:Humans
    5. Mechanotaxis directs Pseudomonas aeruginosa twitching motility. Proc Natl Acad Sci U S A. 2021 07 27; 118(30). Kühn MJ, Talà L, Inclan YF, Patino R, Pierrat X, Vos I, Al-Mayyah Z, Macmillan H, Negrete J, Engel JN, Persat A. PMID: 34301869; PMCID: PMC8325320.
      View in: PubMed   Mentions: 23     Fields:    Translation:Cells
    6. Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi. Nat Microbiol. 2019 02; 4(2):244-250. Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS. PMID: 30617347; PMCID: PMC6424567.
      View in: PubMed   Mentions: 83     Fields:    Translation:Cells
    7. Both the N- and C- terminal regions of the Chlamydial inclusion protein D (IncD) are required for interaction with the pleckstrin homology domain of the ceramide transport protein CERT. Biochem Biophys Res Commun. 2018 11 10; 505(4):1070-1076. Kumagai K, Elwell CA, Ando S, Engel JN, Hanada K. PMID: 30314703; PMCID: PMC6219635.
      View in: PubMed   Mentions: 8     Fields:    Translation:HumansCells
    8. Emerging Role of Retromer in Modulating Pathogen Growth. Trends Microbiol. 2018 09; 26(9):769-780. Elwell C, Engel J. PMID: 29703496; PMCID: PMC6097928.
      View in: PubMed   Mentions: 15     Fields:    Translation:Cells
    9. Targeting the Mucosal Barrier: How Pathogens Modulate the Cellular Polarity Network. Cold Spring Harb Perspect Biol. 2017 Jun 01; 9(6). Ruch TR, Engel JN. PMID: 28193722; PMCID: PMC5453387.
      View in: PubMed   Mentions: 19     Fields:    Translation:HumansAnimalsCells
    10. Chlamydia interfere with an interaction between the mannose-6-phosphate receptor and sorting nexins to counteract host restriction. Elife. 2017 03 02; 6. Elwell CA, Czudnochowski N, von Dollen J, Johnson JR, Nakagawa R, Mirrashidi K, Krogan NJ, Engel JN, Rosenberg OS. PMID: 28252385; PMCID: PMC5364026.
      View in: PubMed   Mentions: 44     Fields:    Translation:AnimalsCells
    11. Bacterial internalization is required to trigger NIK-dependent NF-κB activation in response to the bacterial type three secretion system. PLoS One. 2017; 12(2):e0171406. Duncan MC, Herrera NG, Johnson KS, Engel JN, Auerbuch V. PMID: 28166267; PMCID: PMC5293232.
      View in: PubMed   Mentions:    Fields:    Translation:HumansCells
    12. Par3 integrates Tiam1 and phosphatidylinositol 3-kinase signaling to change apical membrane identity. Mol Biol Cell. 2017 01 15; 28(2):252-260. Ruch TR, Bryant DM, Mostov KE, Engel JN. PMID: 27881661; PMCID: PMC5231894.
      View in: PubMed   Mentions: 6     Fields:    Translation:HumansAnimalsCells
    13. A scaffold protein connects type IV pili with the Chp chemosensory system to mediate activation of virulence signaling in Pseudomonas aeruginosa. Mol Microbiol. 2016 08; 101(4):590-605. Inclan YF, Persat A, Greninger A, Von Dollen J, Johnson J, Krogan N, Gitai Z, Engel JN. PMID: 27145134; PMCID: PMC4980298.
      View in: PubMed   Mentions: 41     Fields:    Translation:Cells
    14. Chlamydia cell biology and pathogenesis. Nat Rev Microbiol. 2016 06; 14(6):385-400. Elwell C, Mirrashidi K, Engel J. PMID: 27108705; PMCID: PMC4886739.
      View in: PubMed   Mentions: 248     Fields:    Translation:HumansCells
    15. Global Mapping of the Inc-Human Interactome Reveals that Retromer Restricts Chlamydia Infection. Cell Host Microbe. 2015 Jul 08; 18(1):109-21. Mirrashidi KM, Elwell CA, Verschueren E, Johnson JR, Frando A, Von Dollen J, Rosenberg O, Gulbahce N, Jang G, Johnson T, Jäger S, Gopalakrishnan AM, Sherry J, Dunn JD, Olive A, Penn B, Shales M, Cox JS, Starnbach MN, Derre I, Valdivia R, Krogan NJ, Engel J. PMID: 26118995; PMCID: PMC4540348.
      View in: PubMed   Mentions: 110     Fields:    Translation:HumansCells
    16. Type IV pili mechanochemically regulate virulence factors in Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 2015 Jun 16; 112(24):7563-8. Persat A, Inclan YF, Engel JN, Stone HA, Gitai Z. PMID: 26041805; PMCID: PMC4475988.
      View in: PubMed   Mentions: 173     Fields:    Translation:Cells
    17. The Pseudomonas aeruginosa type III translocon is required for biofilm formation at the epithelial barrier. PLoS Pathog. 2014 Nov; 10(11):e1004479. Tran CS, Rangel SM, Almblad H, Kierbel A, Givskov M, Tolker-Nielsen T, Hauser AR, Engel JN. PMID: 25375398; PMCID: PMC4223071.
      View in: PubMed   Mentions: 28     Fields:    Translation:AnimalsCells
    18. Host cell polarity proteins participate in innate immunity to Pseudomonas aeruginosa infection. Cell Host Microbe. 2014 May 14; 15(5):636-43. Tran CS, Eran Y, Ruch TR, Bryant DM, Datta A, Brakeman P, Kierbel A, Wittmann T, Metzger RJ, Mostov KE, Engel JN. PMID: 24832456; PMCID: PMC4062193.
      View in: PubMed   Mentions: 29     Fields:    Translation:HumansCells
    19. Cystic fibrosis epithelial cell and bacterial binding. Am J Physiol Lung Cell Mol Physiol. 2013 Dec; 305(11):L891. Engel J, Matthay M. PMID: 24310120.
      View in: PubMed   Mentions:    Fields:    Translation:HumansAnimalsCells
    20. Sugar administration is an effective adjunctive therapy in the treatment of Pseudomonas aeruginosa pneumonia. Am J Physiol Lung Cell Mol Physiol. 2013 Sep; 305(5):L352-63. Bucior I, Abbott J, Song Y, Matthay MA, Engel JN. PMID: 23792737; PMCID: PMC3763031.
      View in: PubMed   Mentions: 19     Fields:    Translation:HumansAnimalsCells
    21. Chlamydial intracellular survival strategies. Cold Spring Harb Perspect Med. 2013 May 01; 3(5):a010256. Bastidas RJ, Elwell CA, Engel JN, Valdivia RH. PMID: 23637308; PMCID: PMC3633179.
      View in: PubMed   Mentions: 102     Fields:    Translation:HumansAnimalsCells
    22. Endosulfatases SULF1 and SULF2 limit Chlamydia muridarum infection. Cell Microbiol. 2013 Sep; 15(9):1560-71. Kim JH, Chan C, Elwell C, Singer MS, Dierks T, Lemjabbar-Alaoui H, Rosen SD, Engel JN. PMID: 23480519; PMCID: PMC3722241.
      View in: PubMed   Mentions: 4     Fields:    Translation:HumansAnimalsCells
    23. Lipid acquisition by intracellular Chlamydiae. Cell Microbiol. 2012 Jul; 14(7):1010-8. Elwell CA, Engel JN. PMID: 22452394; PMCID: PMC3376245.
      View in: PubMed   Mentions: 60     Fields:    Translation:HumansCells
    24. Pseudomonas aeruginosa pili and flagella mediate distinct binding and signaling events at the apical and basolateral surface of airway epithelium. PLoS Pathog. 2012; 8(4):e1002616. Bucior I, Pielage JF, Engel JN. PMID: 22496644; PMCID: PMC3320588.
      View in: PubMed   Mentions: 102     Fields:    Translation:HumansCells
    25. Chlamydia trachomatis co-opts the FGF2 signaling pathway to enhance infection. PLoS Pathog. 2011 Oct; 7(10):e1002285. Kim JH, Jiang S, Elwell CA, Engel JN. PMID: 21998584; PMCID: PMC3188521.
      View in: PubMed   Mentions: 35     Fields:    Translation:HumansCells
    26. Chlamydia trachomatis co-opts GBF1 and CERT to acquire host sphingomyelin for distinct roles during intracellular development. PLoS Pathog. 2011 Sep; 7(9):e1002198. Elwell CA, Jiang S, Kim JH, Lee A, Wittmann T, Hanada K, Melancon P, Engel JN. PMID: 21909260; PMCID: PMC3164637.
      View in: PubMed   Mentions: 120     Fields:    Translation:HumansCells
    27. Subversion of mucosal barrier polarity by pseudomonas aeruginosa. Front Microbiol. 2011; 2:114. Engel J, Eran Y. PMID: 21747810; PMCID: PMC3129012.
      View in: PubMed   Mentions: 26  
    28. Species-specific interactions of Src family tyrosine kinases regulate Chlamydia intracellular growth and trafficking. mBio. 2011; 2(3):e00082-11. Elwell CA, Kierbel A, Engel JN. PMID: 21586644; PMCID: PMC3101784.
      View in: PubMed   Mentions: 3     Fields:    Translation:HumansCells
    29. Cell migration regulates the kinetics of cytokinesis. Cell Cycle. 2011 Feb 15; 10(4):648-54. Wood S, Sivaramakrishnan G, Engel J, Shafikhani SH. PMID: 21293189; PMCID: PMC3174005.
      View in: PubMed   Mentions: 13     Fields:    Translation:HumansCells
    30. FimL regulates cAMP synthesis in Pseudomonas aeruginosa. PLoS One. 2011 Jan 11; 6(1):e15867. Inclan YF, Huseby MJ, Engel JN. PMID: 21264306; PMCID: PMC3019171.
      View in: PubMed   Mentions: 21     Fields:    Translation:HumansCells
    31. Pseudomonas aeruginosa-mediated damage requires distinct receptors at the apical and basolateral surfaces of the polarized epithelium. Infect Immun. 2010 Mar; 78(3):939-53. Bucior I, Mostov K, Engel JN. PMID: 20008530; PMCID: PMC2825949.
      View in: PubMed   Mentions: 46     Fields:    Translation:HumansAnimalsCells
    32. Genetic analysis of the regulation of type IV pilus function by the Chp chemosensory system of Pseudomonas aeruginosa. J Bacteriol. 2010 Feb; 192(4):994-1010. Bertrand JJ, West JT, Engel JN. PMID: 20008072; PMCID: PMC2812951.
      View in: PubMed   Mentions: 66     Fields:    Translation:Cells
    33. CbpA: a polarly localized novel cyclic AMP-binding protein in Pseudomonas aeruginosa. J Bacteriol. 2009 Dec; 191(23):7193-205. Endoh T, Engel JN. PMID: 19801409; PMCID: PMC2786554.
      View in: PubMed   Mentions: 9     Fields:    Translation:AnimalsCells
    34. Role of Pseudomonas aeruginosa type III effectors in disease. Curr Opin Microbiol. 2009 Feb; 12(1):61-6. Engel J, Balachandran P. PMID: 19168385.
      View in: PubMed   Mentions: 145     Fields:    Translation:HumansCells
    35. Focal adhesion components are essential for mammalian cell cytokinesis. Cell Cycle. 2008 Sep 15; 7(18):2868-76. Shafikhani SH, Mostov K, Engel J. PMID: 18787414; PMCID: PMC2745397.
      View in: PubMed   Mentions: 14     Fields:    Translation:HumansAnimalsCells
    36. Roles of type IV pili, flagellum-mediated motility and extracellular DNA in the formation of mature multicellular structures in Pseudomonas aeruginosa biofilms. Environ Microbiol. 2008 Sep; 10(9):2331-43. Barken KB, Pamp SJ, Yang L, Gjermansen M, Bertrand JJ, Klausen M, Givskov M, Whitchurch CB, Engel JN, Tolker-Nielsen T. PMID: 18485000.
      View in: PubMed   Mentions: 167     Fields:    Translation:Cells
    37. RNAi screen reveals an Abl kinase-dependent host cell pathway involved in Pseudomonas aeruginosa internalization. PLoS Pathog. 2008 Mar 21; 4(3):e1000031. Pielage JF, Powell KR, Kalman D, Engel JN. PMID: 18369477; PMCID: PMC2265438.
      View in: PubMed   Mentions: 38     Fields:    Translation:AnimalsCells
    38. RNA interference screen identifies Abl kinase and PDGFR signaling in Chlamydia trachomatis entry. PLoS Pathog. 2008 Mar 07; 4(3):e1000021. Elwell CA, Ceesay A, Kim JH, Kalman D, Engel JN. PMID: 18369471; PMCID: PMC2267011.
      View in: PubMed   Mentions: 89     Fields:    Translation:HumansAnimalsCells
    39. The Pseudomonas aeruginosa type III secreted toxin ExoT is necessary and sufficient to induce apoptosis in epithelial cells. Cell Microbiol. 2008 Apr; 10(4):994-1007. Shafikhani SH, Morales C, Engel J. PMID: 18053004; PMCID: PMC10952005.
      View in: PubMed   Mentions: 40     Fields:    Translation:HumansCells
    40. Use of a novel coinfection system reveals a role for Rac1, H-Ras, and CrkII phosphorylation in Helicobacter pylori-induced host cell actin cytoskeletal rearrangements. FEMS Immunol Med Microbiol. 2007 Jul; 50(2):190-205. Brandt S, Shafikhani S, Balachandran P, Jin S, Hartig R, König W, Engel J, Backert S. PMID: 17428306.
      View in: PubMed   Mentions: 13     Fields:    Translation:HumansCells
    41. Pseudomonas aeruginosa exploits a PIP3-dependent pathway to transform apical into basolateral membrane. J Cell Biol. 2007 Apr 09; 177(1):21-7. Kierbel A, Gassama-Diagne A, Rocha C, Radoshevich L, Olson J, Mostov K, Engel J. PMID: 17403925; PMCID: PMC2064102.
      View in: PubMed   Mentions: 52     Fields:    Translation:AnimalsCells
    42. Copper and nitric oxide meet in the plasma. Nat Chem Biol. 2006 Sep; 2(9):452-3. Samuel TK, Gitlin JD. PMID: 16921354.
      View in: PubMed   Mentions: 3     Fields:    Translation:Cells
    43. Drosophila melanogaster S2 cells: a model system to study Chlamydia interaction with host cells. Cell Microbiol. 2005 May; 7(5):725-39. Elwell C, Engel JN. PMID: 15839901; PMCID: PMC1236988.
      View in: PubMed   Mentions: 33     Fields:    Translation:HumansAnimalsCells
    44. An essential, putative MEK kinase of Leishmania major. Mol Biochem Parasitol. 2005 Jul; 142(1):121-5. Agron PG, Reed SL, Engel JN. PMID: 15878208.
      View in: PubMed   Mentions: 4     Fields:    Translation:Animals
    45. The phosphoinositol-3-kinase-protein kinase B/Akt pathway is critical for Pseudomonas aeruginosa strain PAK internalization. Mol Biol Cell. 2005 May; 16(5):2577-85. Kierbel A, Gassama-Diagne A, Mostov K, Engel JN. PMID: 15772151; PMCID: PMC1087259.
      View in: PubMed   Mentions: 71     Fields:    Translation:HumansAnimalsCells
    46. Pseudomonas aeruginosa fimL regulates multiple virulence functions by intersecting with Vfr-modulated pathways. Mol Microbiol. 2005 Mar; 55(5):1357-78. Whitchurch CB, Beatson SA, Comolli JC, Jakobsen T, Sargent JL, Bertrand JJ, West J, Klausen M, Waite LL, Kang PJ, Tolker-Nielsen T, Mattick JS, Engel JN. PMID: 15720546; PMCID: PMC1266277.
      View in: PubMed   Mentions: 41     Fields:    Translation:Cells
    47. Lipid raft-mediated entry is not required for Chlamydia trachomatis infection of cultured epithelial cells. Infect Immun. 2004 Dec; 72(12):7367-73. Gabel BR, Elwell C, van Ijzendoorn SC, Engel JN. PMID: 15557670; PMCID: PMC529103.
      View in: PubMed   Mentions: 20     Fields:    Translation:HumansCells
    48. A mycobacterial virulence gene cluster extending RD1 is required for cytolysis, bacterial spreading and ESAT-6 secretion. Mol Microbiol. 2004 Sep; 53(6):1677-93. Gao LY, Guo S, McLaughlin B, Morisaki H, Engel JN, Brown EJ. PMID: 15341647.
      View in: PubMed   Mentions: 190     Fields:    Translation:HumansAnimalsCells
    49. Tarp and Arp: How Chlamydia induces its own entry. Proc Natl Acad Sci U S A. 2004 Jul 06; 101(27):9947-8. Engel J. PMID: 15226494; PMCID: PMC454194.
      View in: PubMed   Mentions: 18     Fields:    Translation:Cells
    50. Characterization of a complex chemosensory signal transduction system which controls twitching motility in Pseudomonas aeruginosa. Mol Microbiol. 2004 May; 52(3):873-93. Whitchurch CB, Leech AJ, Young MD, Kennedy D, Sargent JL, Bertrand JJ, Semmler AB, Mellick AS, Martin PR, Alm RA, Hobbs M, Beatson SA, Huang B, Nguyen L, Commolli JC, Engel JN, Darzins A, Mattick JS. PMID: 15101991.
      View in: PubMed   Mentions: 106     Fields:    Translation:AnimalsCells
    51. The ADP ribosyltransferase domain of Pseudomonas aeruginosa ExoT contributes to its biological activities. Infect Immun. 2004 Jan; 72(1):546-58. Garrity-Ryan L, Shafikhani S, Balachandran P, Nguyen L, Oza J, Jakobsen T, Sargent J, Fang X, Cordwell S, Matthay MA, Engel JN. PMID: 14688136; PMCID: PMC343945.
      View in: PubMed   Mentions: 44     Fields:    Translation:HumansAnimalsCells
    52. Effector ExoU from the Type III Secretion System Is An Important Modulator of Gene Expression in Lung Epithelial Cells in Response to Pseudomonas aeruginosa Infection. Infection and Immunity. 2003 Dec 1; 71(12):7240-7240. McMorran MB, Town TL, Costelloe CE, Palmer PJ, Engel EJ, Hume HD, Wainwright WB. .
      View in: Publisher Site   Mentions:
    53. Epithelial cell polarity alters Rho-GTPase responses to Pseudomonas aeruginosa. Mol Biol Cell. 2004 Feb; 15(2):411-9. Kazmierczak BI, Mostov K, Engel JN. PMID: 14595106; PMCID: PMC329196.
      View in: PubMed   Mentions: 23     Fields:    Translation:HumansAnimalsCells
    54. Effector ExoU from the type III secretion system is an important modulator of gene expression in lung epithelial cells in response to Pseudomonas aeruginosa infection. Infect Immun. 2003 Oct; 71(10):6035-44. McMorran B, Town L, Costelloe E, Palmer J, Engel J, Hume D, Wainwright B. PMID: 14500525; PMCID: PMC201109.
      View in: PubMed   Mentions: 17     Fields:    Translation:HumansCells
    55. Molecular Pathogenesis of Acute Pseudomonas Aeruginosa Infections. Severe Infections Caused by Pseudomonas Aeruginosa. 2003 Jan 1; 7:201-229. Engel EJ. .
      View in: Publisher Site   Mentions:
    56. Pseudomonas aeruginosa ExoT acts in vivo as a GTPase-activating protein for RhoA, Rac1, and Cdc42. Infect Immun. 2002 Apr; 70(4):2198-205. Kazmierczak BI, Engel JN. PMID: 11895987; PMCID: PMC127837.
      View in: PubMed   Mentions: 42     Fields:    Translation:HumansAnimalsCells
    57. Type III protein secretion is associated with poor clinical outcomes in patients with ventilator-associated pneumonia caused by Pseudomonas aeruginosa. Crit Care Med. 2002 Mar; 30(3):521-8. Hauser AR, Cobb E, Bodi M, Mariscal D, Vallés J, Engel JN, Rello J. PMID: 11990909.
      View in: PubMed   Mentions: 199     Fields:    Translation:HumansCells
    58. Pseudomonas aeruginosa ExoT inhibits in vitro lung epithelial wound repair. Cell Microbiol. 2001 Apr; 3(4):223-36. Geiser TK, Kazmierczak BI, Garrity-Ryan LK, Matthay MA, Engel JN. PMID: 11298646.
      View in: PubMed   Mentions: 26     Fields:    Translation:HumansCells
    59. Rho GTPase activity modulates Pseudomonas aeruginosa internalization by epithelial cells. Cell Microbiol. 2001 Feb; 3(2):85-98. Kazmierczak BI, Jou TS, Mostov K, Engel JN. PMID: 11207623.
      View in: PubMed   Mentions: 28     Fields:    Translation:HumansAnimalsCells
    60. Interaction of bacterial pathogens with polarized epithelium. Annu Rev Microbiol. 2001; 55:407-35. Kazmierczak BI, Mostov K, Engel JN. PMID: 11544362.
      View in: PubMed   Mentions: 37     Fields:    Translation:Cells
    61. The arginine finger domain of ExoT contributes to actin cytoskeleton disruption and inhibition of internalization of Pseudomonas aeruginosa by epithelial cells and macrophages. Infect Immun. 2000 Dec; 68(12):7100-13. Garrity-Ryan L, Kazmierczak B, Kowal R, Comolli J, Hauser A, Engel JN. PMID: 11083836; PMCID: PMC97821.
      View in: PubMed   Mentions: 86     Fields:    Translation:HumansAnimalsCells
    62. Host cell-derived sphingolipids are required for the intracellular growth of Chlamydia trachomatis. Cell Microbiol. 2000 Dec; 2(6):627-37. van Ooij C, Kalman L, Nishijima M, Hanada K, Mostov K, Engel JN. PMID: 11207614.
      View in: PubMed   Mentions: 57     Fields:    Translation:HumansAnimalsCells
    63. Eukaryotic cell uptake of heparin-coated microspheres: a model of host cell invasion by Chlamydia trachomatis. Infect Immun. 2000 Mar; 68(3):1080-5. Stephens RS, Fawaz FS, Kennedy KA, Koshiyama K, Nichols B, van Ooij C, Engel JN. PMID: 10678910; PMCID: PMC97251.
      View in: PubMed   Mentions: 3     Fields:    Translation:HumansCells
    64. Pseudomonas aeruginosa induces type-III-secretion-mediated apoptosis of macrophages and epithelial cells. Infect Immun. 1999 Oct; 67(10):5530-7. Hauser AR, Engel JN. PMID: 10496945; PMCID: PMC96920.
      View in: PubMed   Mentions: 61     Fields:    Translation:HumansAnimalsCells
    65. Pili binding to asialo-GM1 on epithelial cells can mediate cytotoxicity or bacterial internalization by Pseudomonas aeruginosa. Infect Immun. 1999 Jul; 67(7):3207-14. Comolli JC, Waite LL, Mostov KE, Engel JN. PMID: 10377092; PMCID: PMC116497.
      View in: PubMed   Mentions: 51     Fields:    Translation:AnimalsCells
    66. Pseudomonas aeruginosa gene products PilT and PilU are required for cytotoxicity in vitro and virulence in a mouse model of acute pneumonia. Infect Immun. 1999 Jul; 67(7):3625-30. Comolli JC, Hauser AR, Waite L, Whitchurch CB, Mattick JS, Engel JN. PMID: 10377148; PMCID: PMC116553.
      View in: PubMed   Mentions: 115     Fields:    Translation:AnimalsCells
    67. Fusion of Chlamydia trachomatis-containing inclusions is inhibited at low temperatures and requires bacterial protein synthesis. Infect Immun. 1998 Nov; 66(11):5364-71. Van Ooij C, Homola E, Kincaid E, Engel J. PMID: 9784545; PMCID: PMC108671.
      View in: PubMed   Mentions: 10     Fields:    Translation:HumansAnimalsCells
    68. Mutational analysis of the Chlamydia trachomatis rRNA P1 promoter defines four regions important for transcription in vitro. J Bacteriol. 1998 May; 180(9):2359-66. Tan M, Gaal T, Gourse RL, Engel JN. PMID: 9573186; PMCID: PMC107176.
      View in: PubMed   Mentions: 23     Fields:    Translation:Cells
    69. Defects in type III secretion correlate with internalization of Pseudomonas aeruginosa by epithelial cells. Infect Immun. 1998 Apr; 66(4):1413-20. Hauser AR, Fleiszig S, Kang PJ, Mostov K, Engel JN. PMID: 9529061; PMCID: PMC108068.
      View in: PubMed   Mentions: 43     Fields:    Translation:AnimalsCells
    70. PepA, a secreted protein of Pseudomonas aeruginosa, is necessary for cytotoxicity and virulence. Mol Microbiol. 1998 Feb; 27(4):807-18. Hauser AR, Kang PJ, Engel JN. PMID: 9515706.
      View in: PubMed   Mentions: 102     Fields:    Translation:HumansAnimalsCells
    71. Infection with Chlamydia trachomatis alters the tyrosine phosphorylation and/or localization of several host cell proteins including cortactin. Infect Immun. 1997 Dec; 65(12):5301-8. Fawaz FS, van Ooij C, Homola E, Mutka SC, Engel JN. PMID: 9393830; PMCID: PMC175763.
      View in: PubMed   Mentions: 38     Fields:    Translation:HumansAnimalsCells
    72. Identification of Pseudomonas aeruginosa genes required for epithelial cell injury. Mol Microbiol. 1997 Jun; 24(6):1249-62. Kang PJ, Hauser AR, Apodaca G, Fleiszig SM, Wiener-Kronish J, Mostov K, Engel JN. PMID: 9218773.
      View in: PubMed   Mentions: 60     Fields:    Translation:AnimalsCells
    73. Characterization of the Chlamydia trachomatis vacuole and its interaction with the host endocytic pathway in HeLa cells. Infect Immun. 1997 Feb; 65(2):758-66. van Ooij C, Apodaca G, Engel J. PMID: 9009339; PMCID: PMC176122.
      View in: PubMed   Mentions: 48     Fields:    Translation:HumansCells
    74. Identification of sequences necessary for transcription in vitro from the Chlamydia trachomatis rRNA P1 promoter. J Bacteriol. 1996 Dec; 178(23):6975-82. Tan M, Engel JN. PMID: 8955322; PMCID: PMC178601.
      View in: PubMed   Mentions: 33     Fields:    Translation:Cells
    75. Transcriptional organization and regulation of the dnaK and groE operons of Chlamydia trachomatis. J Bacteriol. 1996 Dec; 178(23):6983-90. Tan M, Wong B, Engel JN. PMID: 8955323; PMCID: PMC178602.
      View in: PubMed   Mentions: 24     Fields:    Translation:Cells
    76. Characterization of Pseudomonas aeruginosa-induced MDCK cell injury: glycosylation-defective host cells are resistant to bacterial killing. Infect Immun. 1995 Apr; 63(4):1541-51. Apodaca G, Bomsel M, Lindstedt R, Engel J, Frank D, Mostov KE, Wiener-Kronish J. PMID: 7890421; PMCID: PMC173187.
      View in: PubMed   Mentions: 53     Fields:    Translation:AnimalsCells
    77. Stage-specific regulation of protein phosphorylation in Leishmania major. Mol Biochem Parasitol. 1994 Apr; 64(2):283-92. Dell KR, Engel JN. PMID: 7935606.
      View in: PubMed   Mentions: 9     Fields:    Translation:AnimalsCells
    78. Cloning and characterization of the RNA polymerase alpha-subunit operon of Chlamydia trachomatis. J Bacteriol. 1993 Nov; 175(22):7150-9. Tan M, Klein R, Grant R, Ganem D, Engel J. PMID: 8226662; PMCID: PMC206856.
      View in: PubMed   Mentions: 2     Fields:    Translation:AnimalsCells
    79. Azithromycin-induced block of elementary body formation in Chlamydia trachomatis. Antimicrob Agents Chemother. 1992 Oct; 36(10):2304-9. Engel JN. PMID: 1280057; PMCID: PMC245493.
      View in: PubMed   Mentions: 4     Fields:    Translation:AnimalsCells
    80. A developmentally regulated chlamydial gene with apparent homology to eukaryotic histone H1. Proc Natl Acad Sci U S A. 1992 Mar 15; 89(6):2125-9. Perara E, Ganem D, Engel JN. PMID: 1549572; PMCID: PMC48609.
      View in: PubMed   Mentions: 35     Fields:    Translation:HumansAnimalsCells
    81. Heat shock response of murine Chlamydia trachomatis. J Bacteriol. 1990 Dec; 172(12):6959-72. Engel JN, Pollack J, Perara E, Ganem D. PMID: 2254267; PMCID: PMC210816.
      View in: PubMed   Mentions: 34     Fields:    Translation:Cells
    82. Cloning and characterization of RNA polymerase core subunits of Chlamydia trachomatis by using the polymerase chain reaction. J Bacteriol. 1990 Oct; 172(10):5732-41. Engel JN, Pollack J, Malik F, Ganem D. PMID: 2211507; PMCID: PMC526889.
      View in: PubMed   Mentions: 12     Fields:    Translation:HumansCells
    83. A polymerase chain reaction-based approach to cloning sigma factors from eubacteria and its application to the isolation of a sigma-70 homolog from Chlamydia trachomatis. J Bacteriol. 1990 May; 172(5):2447-55. Engel JN, Ganem D. PMID: 2110143; PMCID: PMC208882.
      View in: PubMed   Mentions: 25     Fields:    Translation:Cells
    84. 21 Identification and Comparison of Putative Chlamydial Promoter Elements. Immune Recognition and Evasion: Molecular Aspects of Host?parasite Interaction. 1990 Jan 1; 245-260. ENGEL EJ, GANEM GD. .
      View in: Publisher Site   Mentions:
    85. Chlamydial gene encoding a 70-kilodalton antigen in Escherichia coli: analysis of expression signals and identification of the gene product. J Bacteriol. 1989 Jan; 171(1):335-41. Sardinia LM, Engel JN, Ganem D. PMID: 2644193; PMCID: PMC209592.
      View in: PubMed   Mentions: 18     Fields:    Translation:Cells
    86. Chlamydial rRNA operons: gene organization and identification of putative tandem promoters. J Bacteriol. 1987 Dec; 169(12):5678-85. Engel JN, Ganem D. PMID: 2445732; PMCID: PMC214038.
      View in: PubMed   Mentions: 34     Fields:    Translation:Cells
    87. Phenytoin hypersensitivity: a case of severe acute rhabdomyolysis. Am J Med. 1986 Nov; 81(5):928-30. Engel JN, Mellul VG, Goodman DB. PMID: 3776999.
      View in: PubMed   Mentions: 6     Fields:    Translation:Humans
    88. Phenytoin hypersensitivity: a case of severe acute rhabdomyolysis. American Journal of Medicine. 1986; 81:928-930. Engel, JN, Mellul, VG, Goodman, DBP.
    89. Evolutionary conservation in the untranslated regions of actin mRNA's. Nucleic Acids Research. 1984; 12:1687-1696. Ponte, P, Ng, SY, Engel, JN, Kedes, LH.
    90. Isolation and characterization of human actin genes. Ph.D. Dissertation, Stanford University. 1983. Engel, JN.
    91. Isolation and characterization of full-length cDNA clones for human a-, b-, and c- actin mRNA's: skeletal but not cytoplasmic actins have an amino terminal cysteine that is subsequently removed. Molecular and Cellular Biology. 1983; 3:787-795. Gunning, P, Ponte, P, Okayama, H, Engel, JN, Kedes, L.
    92. Molecular genetics of human myogenesis. Experimental Biology and Medicine. 1983; 9:202-212. Kedes, LH, Blau, H, Gunning, P, Ponte, P, Chiu, C-P, Bain, W, Engel, JN, Guttman, S.
    93. Human cytoplasmic actin proteins are encoded by a multigene family. Molecular and Cellular Biology. 1982; 2:674-684. Engel, JN, Gunning, P, Kedes, LH.
    94. Isolation and characterization of human actin genes. Proc Natl Acad Sci U S A. 1981 Aug; 78(8):4674-8. Engel JN, Gunning PW, Kedes L. PMID: 6272269; PMCID: PMC320222.
      View in: PubMed   Mentions: 32     Fields:    Translation:Humans
    95. Isolation and characterization of human actin genes. Proceedings National Academy of Sciences. 1981; 78:4674-4678. Engel, JN, Gunning, P, Kedes, LH.
    96. Inhibition of the in vitro pituitary response to luteinizing hormone-releasing hormone by melatonin, serotonin, and 5-methoxytryptamine. Endocrinology. 1977 Mar; 100(3):675-80. Martin JE, Engel JN, Klein DC. PMID: 401360.
      View in: PubMed   Mentions: 8     Fields:    Translation:Animals
    97. Inhibition of the in vitro pituitary response to luteinizing hormone releasing hormone by melatonin, serotonin, and 5-methoxytryptamine. Endocrinology. 1977; 100:675-680. Martin, JE, Engel, JN, Klein, DC.
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