Orion Weiner, PhD

InstitutionUniversity of California San Francisco
DepartmentCardiovascular Research Inst
Address555 Mission Bay Blvd South
San Francisco CA 94158
vCardDownload vCard

    Collapse Biography 
    Collapse Education and Training
    University of California, San FranciscoPh.D.2001 Graduate Division (Biochemistry & Molecular Biology)

    Collapse Overview 
    Collapse Overview
    Signal transduction networks enable cells to respond to the world around them. To uncover how these signaling networks generate proper responses, we need not only a parts list but also an understanding of how their constituent molecular components work together to process information. I have devoted my lab to developing technologies that will enable us to answer signaling questions that have not been possible to address before. My primary interest is to understand the signaling circuits that organize cell polarity. We take advantage of advanced microscopy techniques in conjunction with techniques to rewire cell signaling or to activate and inhibit signaling on a subcellular level. We aim to move beyond a parts list of components involved in migration to a systems level understanding of cell polarity.

    Collapse Research 
    Collapse Research Activities and Funding
    A toolkit for visualizing and manipulating chromosomal interactions in living cells.
    NIH/NIBIB R21EB022787Jul 1, 2016 - Apr 30, 2018
    Role: Principal Investigator
    Cellular Decision Making
    NIH/NIGMS R35GM118167Jun 1, 2016 - May 31, 2021
    Role: Principal Investigator
    Antigen Decoding by T cells
    NIH/NIGMS R01GM109899Jun 5, 2014 - Feb 28, 2018
    Role: Principal Investigator
    An optogenetic method to rapidly and reversibly titrate protein levels in cells
    NIH/NIBIB R21EB017399Jul 1, 2013 - Jun 30, 2015
    Role: Principal Investigator
    A Toolkit for Light-Control of Molecular Processes in Living Cells
    NIH/NIGMS R01GM096164Mar 1, 2012 - Jan 31, 2016
    Role: Principal Investigator
    Integrative Program in Complex Biological System (ipCBS)
    NIH/NIBIB T32EB009383Apr 1, 2009 - Aug 31, 2019
    Role: Co-Principal Investigator
    Signal Integration in Neutrophil Chemotaxis
    NIH/NIGMS R01GM084040Jun 1, 2008 - May 31, 2017
    Role: Principal Investigator

    Collapse ORNG Applications 
    Collapse Featured Publications
    Collapse Websites
    Collapse In The News

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
    List All   |   Timeline
    1. Saha S, Nagy TL, Weiner O. Joining forces: crosstalk between biochemical signalling and physical forces orchestrates cellular polarity and dynamics. Philos Trans R Soc Lond B Biol Sci. 2018 May 26; 373(1747). PMID: 29632270.
      View in: PubMed
    2. Genuth MA, Allen CDC, Mikawa T, Weiner O. Chick cranial neural crest cells use progressive polarity refinement, not contact inhibition of locomotion, to guide their migration. Dev Biol. 2018 Mar 06. PMID: 29501457.
      View in: PubMed
    3. Yang Y, Xiong D, Pipathsouk A, Weiner O, Wu M. Clathrin Assembly Defines the Onset and Geometry of Cortical Patterning. Dev Cell. 2017 Nov 20; 43(4):507-521.e4. PMID: 29161594.
      View in: PubMed
    4. Graziano BR, Gong D, Anderson KE, Pipathsouk A, Goldberg AR, Weiner O. A module for Rac temporal signal integration revealed with optogenetics. J Cell Biol. 2017 08 07; 216(8):2515-2531. PMID: 28687663.
      View in: PubMed
    5. Reade A, Motta-Mena LB, Gardner KH, Stainier DY, Weiner O, Woo S. TAEL: a zebrafish-optimized optogenetic gene expression system with fine spatial and temporal control. Development. 2017 01 15; 144(2):345-355. PMID: 27993986.
      View in: PubMed
    6. Liu Z, Weiner O. Positioning the cleavage furrow: All you need is Rho. J Cell Biol. 2016 06 20; 213(6):605-7. PMID: 27325786.
      View in: PubMed
    7. Diz-Muñoz A, Thurley K, Chintamen S, Altschuler SJ, Wu LF, Fletcher DA, Weiner O. Membrane Tension Acts Through PLD2 and mTORC2 to Limit Actin Network Assembly During Neutrophil Migration. PLoS Biol. 2016 Jun; 14(6):e1002474. PMID: 27280401; PMCID: PMC4900667.
    8. Hoeller O, Toettcher JE, Cai H, Sun Y, Huang CH, Freyre M, Zhao M, Devreotes PN, Weiner O. Gß Regulates Coupling between Actin Oscillators for Cell Polarity and Directional Migration. PLoS Biol. 2016 Feb; 14(2):e1002381. PMID: 26890004; PMCID: PMC4758609.
    9. Genuth MA, Weiner O. Cell Migration: Recoiling from an Embrace. Curr Biol. 2015 Jun 29; 25(13):R566-8. PMID: 26126284; PMCID: PMC4504735.
    10. Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K, Song Y, Liu S, Murthy Z, Zhang SQ, Weiner O, Kornberg TB, Jan YN, Davidson MW, Shu X. A naturally monomeric infrared fluorescent protein for protein labeling in vivo. Nat Methods. 2015 Aug; 12(8):763-5. PMID: 26098020; PMCID: PMC4521985.
    11. Jost AP, Weiner O. Probing Yeast Polarity with Acute, Reversible, Optogenetic Inhibition of Protein Function. ACS Synth Biol. 2015 Oct 16; 4(10):1077-85. PMID: 26035630; PMCID: PMC4609243 [Available on 10/16/16].
    12. Lou SS, Diz-Muñoz A, Weiner O, Fletcher DA, Theriot JA. Myosin light chain kinase regulates cell polarization independently of membrane tension or Rho kinase. J Cell Biol. 2015 Apr 27; 209(2):275-88. PMID: 25918227; PMCID: PMC4411279.
    13. Wu J, Pipathsouk A, Keizer-Gunnink A, Fusetti F, Alkema W, Liu S, Altschuler S, Wu L, Kortholt A, Weiner O. Homer3 regulates the establishment of neutrophil polarity. Mol Biol Cell. 2015 May 01; 26(9):1629-39. PMID: 25739453; PMCID: PMC4436775.
    14. Tischer D, Weiner O. Illuminating cell signalling with optogenetic tools. Nat Rev Mol Cell Biol. 2014 08; 15(8):551-8. PMID: 25027655; PMCID: PMC4145075.
    15. Graziano BR, Weiner O. Self-organization of protrusions and polarity during eukaryotic chemotaxis. Curr Opin Cell Biol. 2014 Oct; 30:60-7. PMID: 24998184; PMCID: PMC4177965.
    16. Weiner O. Response to Bell et al. Mol Biol Cell. 2014 Jul 01; 25(13):1945. PMID: 24970486; PMCID: PMC4072568.
    17. Park JS, Rhau B, Hermann A, McNally KA, Zhou C, Gong D, Weiner O, Conklin BR, Onuffer J, Lim WA. Synthetic control of mammalian-cell motility by engineering chemotaxis to an orthogonal bioinert chemical signal. Proc Natl Acad Sci U S A. 2014 Apr 22; 111(16):5896-901. PMID: 24711398; PMCID: PMC4000811.
    18. Hoeller O, Gong D, Weiner O. How to understand and outwit adaptation. Dev Cell. 2014 Mar 31; 28(6):607-616. PMID: 24697896; PMCID: PMC4012857.
    19. Weiner O. How should we be selecting our graduate students? Mol Biol Cell. 2014 Feb; 25(4):429-30. PMID: 24525948; PMCID: PMC3923635.
    20. Toettcher JE, Weiner O, Lim WA. Using optogenetics to interrogate the dynamic control of signal transmission by the Ras/Erk module. Cell. 2013 Dec 05; 155(6):1422-34. PMID: 24315106; PMCID: PMC3925772.
    21. Dandekar SN, Park JS, Peng GE, Onuffer JJ, Lim WA, Weiner O. Actin dynamics rapidly reset chemoattractant receptor sensitivity following adaptation in neutrophils. Philos Trans R Soc Lond B Biol Sci. 2013; 368(1629):20130008. PMID: 24062580; PMCID: PMC3785960.
    22. Hoeller O, Bolourani P, Clark J, Stephens LR, Hawkins PT, Weiner O, Weeks G, Kay RR. Two distinct functions for PI3-kinases in macropinocytosis. J Cell Sci. 2013 Sep 15; 126(Pt 18):4296-307. PMID: 23843627; PMCID: PMC3772393.
    23. Yang X, Jost AP, Weiner O, Tang C. A light-inducible organelle-targeting system for dynamically activating and inactivating signaling in budding yeast. Mol Biol Cell. 2013 Aug; 24(15):2419-30. PMID: 23761071; PMCID: PMC3727934.
    24. Wang Y, Ku CJ, Zhang ER, Artyukhin AB, Weiner O, Wu LF, Altschuler SJ. Identifying network motifs that buffer front-to-back signaling in polarized neutrophils. Cell Rep. 2013 May 30; 3(5):1607-16. PMID: 23665220; PMCID: PMC3674638.
    25. Diz-Muñoz A, Fletcher DA, Weiner O. Use the force: membrane tension as an organizer of cell shape and motility. Trends Cell Biol. 2013 Feb; 23(2):47-53. PMID: 23122885; PMCID: PMC3558607.
    26. Woo S, Housley MP, Weiner O, Stainier DY. Nodal signaling regulates endodermal cell motility and actin dynamics via Rac1 and Prex1. J Cell Biol. 2012 Sep 03; 198(5):941-52. PMID: 22945937; PMCID: PMC3432772.
    27. Ku CJ, Wang Y, Weiner O, Altschuler SJ, Wu LF. Network crosstalk dynamically changes during neutrophil polarization. Cell. 2012 May 25; 149(5):1073-83. PMID: 22632971; PMCID: PMC3614011.
    28. Millius A, Watanabe N, Weiner O. Diffusion, capture and recycling of SCAR/WAVE and Arp2/3 complexes observed in cells by single-molecule imaging. J Cell Sci. 2012 Mar 01; 125(Pt 5):1165-76. PMID: 22349699; PMCID: PMC3324580.
    29. Houk AR, Jilkine A, Mejean CO, Boltyanskiy R, Dufresne ER, Angenent SB, Altschuler SJ, Wu LF, Weiner O. Membrane tension maintains cell polarity by confining signals to the leading edge during neutrophil migration. Cell. 2012 Jan 20; 148(1-2):175-88. PMID: 22265410; PMCID: PMC3308728.
    30. Toettcher JE, Gong D, Lim WA, Weiner O. Light-based feedback for controlling intracellular signaling dynamics. Nat Methods. 2011 Sep 11; 8(10):837-9. PMID: 21909100; PMCID: PMC3184382.
    31. Peng GE, Wilson SR, Weiner O. A pharmacological cocktail for arresting actin dynamics in living cells. Mol Biol Cell. 2011 Nov; 22(21):3986-94. PMID: 21880897; PMCID: PMC3204061.
    32. Toettcher JE, Gong D, Lim WA, Weiner O. Light control of plasma membrane recruitment using the Phy-PIF system. Methods Enzymol. 2011; 497:409-23. PMID: 21601096; PMCID: PMC3121701.
    33. Toettcher JE, Voigt CA, Weiner O, Lim WA. The promise of optogenetics in cell biology: interrogating molecular circuits in space and time. Nat Methods. 2011 Jan; 8(1):35-8. PMID: 21191370; PMCID: PMC3024327.
    34. Puthenveedu MA, Lauffer B, Temkin P, Vistein R, Carlton P, Thorn K, Taunton J, Weiner O, Parton RG, von Zastrow M. Sequence-dependent sorting of recycling proteins by actin-stabilized endosomal microdomains. Cell. 2010 Nov 24; 143(5):761-73. PMID: 21111236; PMCID: PMC3058345.
    35. Millius A, Weiner O. Manipulation of neutrophil-like HL-60 cells for the study of directed cell migration. Methods Mol Biol. 2010; 591:147-58. PMID: 19957129; PMCID: PMC3128798.
    36. Kress H, Park JG, Mejean CO, Forster JD, Park J, Walse SS, Zhang Y, Wu D, Weiner O, Fahmy TM, Dufresne ER. Cell stimulation with optically manipulated microsources. Nat Methods. 2009 Dec; 6(12):905-9. PMID: 19915561; PMCID: PMC2813772.
    37. Houk AR, Millius A, Weiner O. Compete globally, bud locally. Cell. 2009 Nov 13; 139(4):656-8. PMID: 19914160; PMCID: PMC2812431.
    38. Levskaya A, Weiner O, Lim WA, Voigt CA. Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Oct 15; 461(7266):997-1001. PMID: 19749742; PMCID: PMC2989900.
    39. Millius A, Dandekar SN, Houk AR, Weiner O. Neutrophils establish rapid and robust WAVE complex polarity in an actin-dependent fashion. Curr Biol. 2009 Feb 10; 19(3):253-9. PMID: 19200726; PMCID: PMC2705202.
    40. Millius A, Weiner O. Chemotaxis in neutrophil-like HL-60 cells. Methods Mol Biol. 2009; 571:167-77. PMID: 19763966; PMCID: PMC2812563.
    41. Weiner O, Marganski WA, Wu LF, Altschuler SJ, Kirschner MW. An actin-based wave generator organizes cell motility. PLoS Biol. 2007 Sep; 5(9):e221. PMID: 17696648; PMCID: PMC1945041.
    42. Pestonjamasp KN, Forster C, Sun C, Gardiner EM, Bohl B, Weiner O, Bokoch GM, Glogauer M. Rac1 links leading edge and uropod events through Rho and myosin activation during chemotaxis. Blood. 2006 Oct 15; 108(8):2814-20. PMID: 16809619; PMCID: PMC1895589.
    43. Weiner O, Rentel MC, Ott A, Brown GE, Jedrychowski M, Yaffe MB, Gygi SP, Cantley LC, Bourne HR, Kirschner MW. Hem-1 complexes are essential for Rac activation, actin polymerization, and myosin regulation during neutrophil chemotaxis. PLoS Biol. 2006 Feb; 4(2):e38. PMID: 16417406; PMCID: PMC1334198.
    44. Wang F, Herzmark P, Weiner O, Srinivasan S, Servant G, Bourne HR. Lipid products of PI(3)Ks maintain persistent cell polarity and directed motility in neutrophils. Nat Cell Biol. 2002 Jul; 4(7):513-8. PMID: 12080345.
      View in: PubMed
    45. Weiner O, Neilsen PO, Prestwich GD, Kirschner MW, Cantley LC, Bourne HR. A PtdInsP(3)- and Rho GTPase-mediated positive feedback loop regulates neutrophil polarity. Nat Cell Biol. 2002 Jul; 4(7):509-13. PMID: 12080346; PMCID: PMC2823287.
    46. Weiner O. Rac activation: P-Rex1 - a convergence point for PIP(3) and Gbetagamma? Curr Biol. 2002 Jun 25; 12(12):R429-31. PMID: 12123595; PMCID: PMC2819105.
    Orion's Networks
    Derived automatically from this person's publications.
    People in Profiles who have published with this person.
    Related Authors
    People who share related concepts with this person.