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Orion Weiner, PhD

TitleProfessor
SchoolUCSF School of Medicine
DepartmentCardiovascular Research Inst
Address555 Mission Bay Blvd South
San Francisco CA 94158
Phone415-514-4508
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    Collapse Biography 
    Collapse Education and Training
    University of California, San FranciscoPh.D.2001 Graduate Division (Biochemistry & Molecular Biology)

    Collapse Overview 
    Collapse Overview
    Signal transduction networks enable cells to respond to the world around them. To uncover how these signaling networks generate proper responses, we need not only a parts list but also an understanding of how their constituent molecular components work together to process information. I have devoted my lab to developing technologies that will enable us to answer signaling questions that have not been possible to address before. My primary interest is to understand the signaling circuits that organize cell polarity. We take advantage of advanced microscopy techniques in conjunction with techniques to rewire cell signaling or to activate and inhibit signaling on a subcellular level. We aim to move beyond a parts list of components involved in migration to a systems level understanding of cell polarity.


    Collapse Research 
    Collapse Research Activities and Funding
    A toolkit for visualizing and manipulating chromosomal interactions in living cells.
    NIH/NIBIB R21EB022787Jul 1, 2016 - Apr 30, 2018
    Role: Principal Investigator
    Cellular Decision Making
    NIH/NIGMS R35GM118167Jun 1, 2016 - May 31, 2021
    Role: Principal Investigator
    Antigen Decoding by T cells
    NIH/NIGMS R01GM109899Jun 5, 2014 - Feb 28, 2018
    Role: Principal Investigator
    An optogenetic method to rapidly and reversibly titrate protein levels in cells
    NIH/NIBIB R21EB017399Jul 1, 2013 - Jun 30, 2015
    Role: Principal Investigator
    A Toolkit for Light-Control of Molecular Processes in Living Cells
    NIH/NIGMS R01GM096164Mar 1, 2012 - Jan 31, 2016
    Role: Principal Investigator
    Integrative Program in Complex Biological System (ipCBS)
    NIH/NIBIB T32EB009383Apr 1, 2009 - Aug 31, 2019
    Role: Co-Principal Investigator
    Signal Integration in Neutrophil Chemotaxis
    NIH/NIGMS R01GM084040Jun 1, 2008 - May 31, 2017
    Role: Principal Investigator

    Collapse ORNG Applications 
    Collapse Websites
    Collapse In The News
    Collapse Global Health
    Collapse Featured Publications

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
    List All   |   Timeline
    1. Saha S, Nagy TL, Weiner O. Joining forces: crosstalk between biochemical signalling and physical forces orchestrates cellular polarity and dynamics. Philos Trans R Soc Lond B Biol Sci. 2018 May 26; 373(1747). PMID: 29632270.
      View in: PubMed
    2. Genuth MA, Allen CDC, Mikawa T, Weiner O. Chick cranial neural crest cells use progressive polarity refinement, not contact inhibition of locomotion, to guide their migration. Dev Biol. 2018 Mar 06. PMID: 29501457.
      View in: PubMed
    3. Yang Y, Xiong D, Pipathsouk A, Weiner O, Wu M. Clathrin Assembly Defines the Onset and Geometry of Cortical Patterning. Dev Cell. 2017 Nov 20; 43(4):507-521.e4. PMID: 29161594.
      View in: PubMed
    4. Graziano BR, Gong D, Anderson KE, Pipathsouk A, Goldberg AR, Weiner O. A module for Rac temporal signal integration revealed with optogenetics. J Cell Biol. 2017 08 07; 216(8):2515-2531. PMID: 28687663.
      View in: PubMed
    5. Reade A, Motta-Mena LB, Gardner KH, Stainier DY, Weiner O, Woo S. TAEL: a zebrafish-optimized optogenetic gene expression system with fine spatial and temporal control. Development. 2017 01 15; 144(2):345-355. PMID: 27993986.
      View in: PubMed
    6. Liu Z, Weiner O. Positioning the cleavage furrow: All you need is Rho. J Cell Biol. 2016 06 20; 213(6):605-7. PMID: 27325786.
      View in: PubMed
    7. Diz-Muñoz A, Thurley K, Chintamen S, Altschuler SJ, Wu LF, Fletcher DA, Weiner O. Membrane Tension Acts Through PLD2 and mTORC2 to Limit Actin Network Assembly During Neutrophil Migration. PLoS Biol. 2016 Jun; 14(6):e1002474. PMID: 27280401; PMCID: PMC4900667.
      View in: PubMed, PubMed Central
    8. Hoeller O, Toettcher JE, Cai H, Sun Y, Huang CH, Freyre M, Zhao M, Devreotes PN, Weiner O. Gß Regulates Coupling between Actin Oscillators for Cell Polarity and Directional Migration. PLoS Biol. 2016 Feb; 14(2):e1002381. PMID: 26890004; PMCID: PMC4758609.
      View in: PubMed, PubMed Central
    9. Buckley CE, Moore RE, Reade A, Goldberg AR, Weiner O, Clarke JD. Reversible Optogenetic Control of Subcellular Protein Localization in a Live Vertebrate Embryo. Dev Cell. 2016 Jan 11; 36(1):117-126. PMID: 26766447; PMCID: PMC4712025.
      View in: PubMed, PubMed Central
    10. Genuth MA, Weiner O. Cell Migration: Recoiling from an Embrace. Curr Biol. 2015 Jun 29; 25(13):R566-8. PMID: 26126284; PMCID: PMC4504735.
      View in: PubMed, PubMed Central
    11. Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K, Song Y, Liu S, Murthy Z, Zhang SQ, Weiner O, Kornberg TB, Jan YN, Davidson MW, Shu X. A naturally monomeric infrared fluorescent protein for protein labeling in vivo. Nat Methods. 2015 Aug; 12(8):763-5. PMID: 26098020; PMCID: PMC4521985.
      View in: PubMed, PubMed Central
    12. Jost AP, Weiner O. Probing Yeast Polarity with Acute, Reversible, Optogenetic Inhibition of Protein Function. ACS Synth Biol. 2015 Oct 16; 4(10):1077-85. PMID: 26035630; PMCID: PMC4609243 [Available on 10/16/16].
      View in: PubMed, PubMed Central
    13. Lou SS, Diz-Muñoz A, Weiner O, Fletcher DA, Theriot JA. Myosin light chain kinase regulates cell polarization independently of membrane tension or Rho kinase. J Cell Biol. 2015 Apr 27; 209(2):275-88. PMID: 25918227; PMCID: PMC4411279.
      View in: PubMed, PubMed Central
    14. Wu J, Pipathsouk A, Keizer-Gunnink A, Fusetti F, Alkema W, Liu S, Altschuler S, Wu L, Kortholt A, Weiner O. Homer3 regulates the establishment of neutrophil polarity. Mol Biol Cell. 2015 May 01; 26(9):1629-39. PMID: 25739453; PMCID: PMC4436775.
      View in: PubMed, PubMed Central
    15. Tischer D, Weiner O. Illuminating cell signalling with optogenetic tools. Nat Rev Mol Cell Biol. 2014 08; 15(8):551-8. PMID: 25027655; PMCID: PMC4145075.
      View in: PubMed, PubMed Central
    16. Graziano BR, Weiner O. Self-organization of protrusions and polarity during eukaryotic chemotaxis. Curr Opin Cell Biol. 2014 Oct; 30:60-7. PMID: 24998184; PMCID: PMC4177965.
      View in: PubMed, PubMed Central
    17. Weiner O. Response to Bell et al. Mol Biol Cell. 2014 Jul 01; 25(13):1945. PMID: 24970486; PMCID: PMC4072568.
      View in: PubMed, PubMed Central
    18. Park JS, Rhau B, Hermann A, McNally KA, Zhou C, Gong D, Weiner O, Conklin BR, Onuffer J, Lim WA. Synthetic control of mammalian-cell motility by engineering chemotaxis to an orthogonal bioinert chemical signal. Proc Natl Acad Sci U S A. 2014 Apr 22; 111(16):5896-901. PMID: 24711398; PMCID: PMC4000811.
      View in: PubMed, PubMed Central
    19. Hoeller O, Gong D, Weiner O. How to understand and outwit adaptation. Dev Cell. 2014 Mar 31; 28(6):607-616. PMID: 24697896; PMCID: PMC4012857.
      View in: PubMed, PubMed Central
    20. Weiner O. How should we be selecting our graduate students? Mol Biol Cell. 2014 Feb; 25(4):429-30. PMID: 24525948; PMCID: PMC3923635.
      View in: PubMed, PubMed Central
    21. Motta-Mena LB, Reade A, Mallory MJ, Glantz S, Weiner O, Lynch KW, Gardner KH. An optogenetic gene expression system with rapid activation and deactivation kinetics. Nat Chem Biol. 2014 Mar; 10(3):196-202. PMID: 24413462; PMCID: PMC3944926.
      View in: PubMed, PubMed Central
    22. Toettcher JE, Weiner O, Lim WA. Using optogenetics to interrogate the dynamic control of signal transmission by the Ras/Erk module. Cell. 2013 Dec 05; 155(6):1422-34. PMID: 24315106; PMCID: PMC3925772.
      View in: PubMed, PubMed Central
    23. Dandekar SN, Park JS, Peng GE, Onuffer JJ, Lim WA, Weiner O. Actin dynamics rapidly reset chemoattractant receptor sensitivity following adaptation in neutrophils. Philos Trans R Soc Lond B Biol Sci. 2013; 368(1629):20130008. PMID: 24062580; PMCID: PMC3785960.
      View in: PubMed, PubMed Central
    24. Parent CA, Weiner O. The symphony of cell movement: how cells orchestrate diverse signals and forces to control migration. Curr Opin Cell Biol. 2013 Oct; 25(5):523-5. PMID: 23927870.
      View in: PubMed
    25. Hoeller O, Bolourani P, Clark J, Stephens LR, Hawkins PT, Weiner O, Weeks G, Kay RR. Two distinct functions for PI3-kinases in macropinocytosis. J Cell Sci. 2013 Sep 15; 126(Pt 18):4296-307. PMID: 23843627; PMCID: PMC3772393.
      View in: PubMed, PubMed Central
    26. Yang X, Jost AP, Weiner O, Tang C. A light-inducible organelle-targeting system for dynamically activating and inactivating signaling in budding yeast. Mol Biol Cell. 2013 Aug; 24(15):2419-30. PMID: 23761071; PMCID: PMC3727934.
      View in: PubMed, PubMed Central
    27. Wang Y, Ku CJ, Zhang ER, Artyukhin AB, Weiner O, Wu LF, Altschuler SJ. Identifying network motifs that buffer front-to-back signaling in polarized neutrophils. Cell Rep. 2013 May 30; 3(5):1607-16. PMID: 23665220; PMCID: PMC3674638.
      View in: PubMed, PubMed Central
    28. Diz-Muñoz A, Fletcher DA, Weiner O. Use the force: membrane tension as an organizer of cell shape and motility. Trends Cell Biol. 2013 Feb; 23(2):47-53. PMID: 23122885; PMCID: PMC3558607.
      View in: PubMed, PubMed Central
    29. Woo S, Housley MP, Weiner O, Stainier DY. Nodal signaling regulates endodermal cell motility and actin dynamics via Rac1 and Prex1. J Cell Biol. 2012 Sep 03; 198(5):941-52. PMID: 22945937; PMCID: PMC3432772.
      View in: PubMed, PubMed Central
    30. Ku CJ, Wang Y, Weiner O, Altschuler SJ, Wu LF. Network crosstalk dynamically changes during neutrophil polarization. Cell. 2012 May 25; 149(5):1073-83. PMID: 22632971; PMCID: PMC3614011.
      View in: PubMed, PubMed Central
    31. Millius A, Watanabe N, Weiner O. Diffusion, capture and recycling of SCAR/WAVE and Arp2/3 complexes observed in cells by single-molecule imaging. J Cell Sci. 2012 Mar 01; 125(Pt 5):1165-76. PMID: 22349699; PMCID: PMC3324580.
      View in: PubMed, PubMed Central
    32. Houk AR, Jilkine A, Mejean CO, Boltyanskiy R, Dufresne ER, Angenent SB, Altschuler SJ, Wu LF, Weiner O. Membrane tension maintains cell polarity by confining signals to the leading edge during neutrophil migration. Cell. 2012 Jan 20; 148(1-2):175-88. PMID: 22265410; PMCID: PMC3308728.
      View in: PubMed, PubMed Central
    33. Toettcher JE, Gong D, Lim WA, Weiner O. Light-based feedback for controlling intracellular signaling dynamics. Nat Methods. 2011 Sep 11; 8(10):837-9. PMID: 21909100; PMCID: PMC3184382.
      View in: PubMed, PubMed Central
    34. Peng GE, Wilson SR, Weiner O. A pharmacological cocktail for arresting actin dynamics in living cells. Mol Biol Cell. 2011 Nov; 22(21):3986-94. PMID: 21880897; PMCID: PMC3204061.
      View in: PubMed, PubMed Central
    35. Toettcher JE, Gong D, Lim WA, Weiner O. Light control of plasma membrane recruitment using the Phy-PIF system. Methods Enzymol. 2011; 497:409-23. PMID: 21601096; PMCID: PMC3121701.
      View in: PubMed, PubMed Central
    36. Toettcher JE, Voigt CA, Weiner O, Lim WA. The promise of optogenetics in cell biology: interrogating molecular circuits in space and time. Nat Methods. 2011 Jan; 8(1):35-8. PMID: 21191370; PMCID: PMC3024327.
      View in: PubMed, PubMed Central
    37. Puthenveedu MA, Lauffer B, Temkin P, Vistein R, Carlton P, Thorn K, Taunton J, Weiner O, Parton RG, von Zastrow M. Sequence-dependent sorting of recycling proteins by actin-stabilized endosomal microdomains. Cell. 2010 Nov 24; 143(5):761-73. PMID: 21111236; PMCID: PMC3058345.
      View in: PubMed, PubMed Central
    38. Millius A, Weiner O. Manipulation of neutrophil-like HL-60 cells for the study of directed cell migration. Methods Mol Biol. 2010; 591:147-58. PMID: 19957129; PMCID: PMC3128798.
      View in: PubMed, PubMed Central
    39. Kress H, Park JG, Mejean CO, Forster JD, Park J, Walse SS, Zhang Y, Wu D, Weiner O, Fahmy TM, Dufresne ER. Cell stimulation with optically manipulated microsources. Nat Methods. 2009 Dec; 6(12):905-9. PMID: 19915561; PMCID: PMC2813772.
      View in: PubMed, PubMed Central
    40. Houk AR, Millius A, Weiner O. Compete globally, bud locally. Cell. 2009 Nov 13; 139(4):656-8. PMID: 19914160; PMCID: PMC2812431.
      View in: PubMed, PubMed Central
    41. Levskaya A, Weiner O, Lim WA, Voigt CA. Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Oct 15; 461(7266):997-1001. PMID: 19749742; PMCID: PMC2989900.
      View in: PubMed, PubMed Central
    42. Millius A, Dandekar SN, Houk AR, Weiner O. Neutrophils establish rapid and robust WAVE complex polarity in an actin-dependent fashion. Curr Biol. 2009 Feb 10; 19(3):253-9. PMID: 19200726; PMCID: PMC2705202.
      View in: PubMed, PubMed Central
    43. Millius A, Weiner O. Chemotaxis in neutrophil-like HL-60 cells. Methods Mol Biol. 2009; 571:167-77. PMID: 19763966; PMCID: PMC2812563.
      View in: PubMed, PubMed Central
    44. Weiner O, Marganski WA, Wu LF, Altschuler SJ, Kirschner MW. An actin-based wave generator organizes cell motility. PLoS Biol. 2007 Sep; 5(9):e221. PMID: 17696648; PMCID: PMC1945041.
      View in: PubMed, PubMed Central
    45. Pestonjamasp KN, Forster C, Sun C, Gardiner EM, Bohl B, Weiner O, Bokoch GM, Glogauer M. Rac1 links leading edge and uropod events through Rho and myosin activation during chemotaxis. Blood. 2006 Oct 15; 108(8):2814-20. PMID: 16809619; PMCID: PMC1895589.
      View in: PubMed, PubMed Central
    46. Weiner O, Rentel MC, Ott A, Brown GE, Jedrychowski M, Yaffe MB, Gygi SP, Cantley LC, Bourne HR, Kirschner MW. Hem-1 complexes are essential for Rac activation, actin polymerization, and myosin regulation during neutrophil chemotaxis. PLoS Biol. 2006 Feb; 4(2):e38. PMID: 16417406; PMCID: PMC1334198.
      View in: PubMed, PubMed Central
    47. Bourne HR, Weiner O. A chemical compass. Nature. 2002 Sep 05; 419(6902):21. PMID: 12214215.
      View in: PubMed
    48. Wang F, Herzmark P, Weiner O, Srinivasan S, Servant G, Bourne HR. Lipid products of PI(3)Ks maintain persistent cell polarity and directed motility in neutrophils. Nat Cell Biol. 2002 Jul; 4(7):513-8. PMID: 12080345.
      View in: PubMed
    49. Weiner O, Neilsen PO, Prestwich GD, Kirschner MW, Cantley LC, Bourne HR. A PtdInsP(3)- and Rho GTPase-mediated positive feedback loop regulates neutrophil polarity. Nat Cell Biol. 2002 Jul; 4(7):509-13. PMID: 12080346; PMCID: PMC2823287.
      View in: PubMed, PubMed Central
    50. Weiner O. Rac activation: P-Rex1 - a convergence point for PIP(3) and Gbetagamma? Curr Biol. 2002 Jun 25; 12(12):R429-31. PMID: 12123595; PMCID: PMC2819105.
      View in: PubMed, PubMed Central
    51. Weiner O. Regulation of cell polarity during eukaryotic chemotaxis: the chemotactic compass. Curr Opin Cell Biol. 2002 Apr; 14(2):196-202. PMID: 11891119; PMCID: PMC2819118.
      View in: PubMed, PubMed Central
    52. Insall RH, Weiner O. PIP3, PIP2, and cell movement--similar messages, different meanings? Dev Cell. 2001 Dec; 1(6):743-7. PMID: 11740936; PMCID: PMC2819114.
      View in: PubMed, PubMed Central
    53. Rickert P, Weiner O, Wang F, Bourne HR, Servant G. Leukocytes navigate by compass: roles of PI3Kgamma and its lipid products. Trends Cell Biol. 2000 Nov; 10(11):466-73. PMID: 11050418; PMCID: PMC2819116.
      View in: PubMed, PubMed Central
    54. Servant G, Weiner O, Herzmark P, Balla T, Sedat JW, Bourne HR. Polarization of chemoattractant receptor signaling during neutrophil chemotaxis. Science. 2000 Feb 11; 287(5455):1037-40. PMID: 10669415; PMCID: PMC2822871.
      View in: PubMed, PubMed Central
    55. Kalman D, Weiner O, Goosney DL, Sedat JW, Finlay BB, Abo A, Bishop JM. Enteropathogenic E. coli acts through WASP and Arp2/3 complex to form actin pedestals. Nat Cell Biol. 1999 Oct; 1(6):389-91. PMID: 10559969; PMCID: PMC2828053.
      View in: PubMed, PubMed Central
    56. Weiner O, Servant G, Welch MD, Mitchison TJ, Sedat JW, Bourne HR. Spatial control of actin polymerization during neutrophil chemotaxis. Nat Cell Biol. 1999 Jun; 1(2):75-81. PMID: 10559877; PMCID: PMC2828058.
      View in: PubMed, PubMed Central
    57. Servant G, Weiner O, Neptune ER, Sedat JW, Bourne HR. Dynamics of a chemoattractant receptor in living neutrophils during chemotaxis. Mol Biol Cell. 1999 Apr; 10(4):1163-78. PMID: 10198064; PMCID: PMC25243.
      View in: PubMed, PubMed Central
    58. Weiner O, Zorn AM, Krieg PA, Bittner GD. Medium weight neurofilament mRNA in goldfish Mauthner axoplasm. Neurosci Lett. 1996 Aug 02; 213(2):83-6. PMID: 8858614; PMCID: PMC2830807.
      View in: PubMed, PubMed Central
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