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Wendell Lim, PhD

TitleProfessor
SchoolUCSF School of Medicine
DepartmentCellular Molecular Pharmacology
Address600 16th St
San Francisco CA 94158
Phone415-502-8080
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    Other Positions
    TitleHoward Hughes Investigator


    Collapse Research 
    Collapse Research Activities and Funding
    Redesigning the T cell: Using Synthetic Biology to Engineer Therapeutic Cells
    NIH/NCI R01CA196277Sep 23, 2014 - Aug 31, 2019
    Role: Principal Investigator
    Harnessing CRISPR for Targeted and Inducible Epigenomic Reprogramming
    NIH/NIDA R01DA036858Sep 30, 2013 - May 31, 2018
    Role: Principal Investigator
    Exploring Design Principles of Cellular Control Circuits
    NIH/NIGMS P50GM081879Aug 1, 2010 - Jul 31, 2018
    Role: Principal Investigator
    Cellular Control: Synthetic Signaling/Motility (RMI)
    NIH/NEI PN2EY016546Sep 30, 2004 - Jul 31, 2015
    Role: Principal Investigator
    Engineering Synthetic Cell Motility Systems (RMI)
    NIH/NEI PN1EY016546Sep 30, 2004 - Jul 31, 2005
    Role: Principal Investigator
    ASSEMBLY AND TARGETING OF NEURAL RECEPTORS AND CHANNELS
    NIH/NIMH P50MH063981Aug 15, 2001 - Jul 31, 2007
    Role: Co-Investigator
    Protein Interactions Regulating Cell Motility
    NIH/NIGMS R01GM062583Feb 1, 2001 - Apr 30, 2012
    Role: Principal Investigator
    Protein Recognition in Signal Transduction
    NIH/NIGMS R01GM055040Jan 1, 1997 - Jul 31, 2016
    Role: Principal Investigator
    Bio-Organic Biomedical Mass Spectrometry Resource
    NIH P41RR001614Mar 1, 1982 - May 31, 2015
    Role: Co-Investigator

    Collapse ORNG Applications 
    Collapse Websites
    Collapse In The News
    Collapse Global Health

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
    List All   |   Timeline
    1. Bugaj LJ, O'Donoghue GP, Lim W. Interrogating cellular perception and decision making with optogenetic tools. J Cell Biol. 2016 Dec 21. PMID: 28003330.
      View in: PubMed
    2. Esensten JH, Bluestone JA, Lim W. Engineering Therapeutic T Cells: From Synthetic Biology to Clinical Trials. Annu Rev Pathol. 2016 Dec 05. PMID: 27959633.
      View in: PubMed
    3. Gordley RM, Williams RE, Bashor CJ, Toettcher JE, Yan S, Lim W. Engineering dynamical control of cell fate switching using synthetic phospho-regulons. Proc Natl Acad Sci U S A. 2016 Nov 7. PMID: 27821768.
      View in: PubMed
    4. Gao Y, Xiong X, Wong S, Charles EJ, Lim W, Qi LS. Complex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods. 2016 Oct 24. PMID: 27776111.
      View in: PubMed
    5. Roybal KT, Williams JZ, Morsut L, Rupp LJ, Kolinko I, Choe JH, Walker WJ, McNally KA, Lim W. Engineering T Cells with Customized Therapeutic Response Programs Using Synthetic Notch Receptors. Cell. 2016 Oct 6; 167(2):419-432.e16. PMID: 27693353.
      View in: PubMed
    6. Mitchell A, Lim W. Cellular perception and misperception: Internal models for decision-making shaped by evolutionary experience. Bioessays. 2016 Sep; 38(9):845-9. PMID: 27461864.
      View in: PubMed
    7. Gordley RM, Bugaj LJ, Lim W. Modular engineering of cellular signaling proteins and networks. Curr Opin Struct Biol. 2016 Aug; 39:106-114. PMID: 27423114.
      View in: PubMed
    8. Xiong X, Chen M, Lim W, Zhao D, Qi LS. CRISPR/Cas9 for Human Genome Engineering and Disease Research. Annu Rev Genomics Hum Genet. 2016 Aug 31; 17:131-54. PMID: 27216776.
      View in: PubMed
    9. Isaac RS, Jiang F, Doudna JA, Lim W, Narlikar GJ, Almeida R. Nucleosome breathing and remodeling constrain CRISPR-Cas9 function. Elife. 2016; 5. PMID: 27130520.
      View in: PubMed
    10. Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim W. Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Cell. 2016 Feb 11; 164(4):780-91. PMID: 26830878.
      View in: PubMed
    11. Roybal KT, Rupp LJ, Morsut L, Walker WJ, McNally KA, Park JS, Lim W. Precision Tumor Recognition by T Cells With Combinatorial Antigen-Sensing Circuits. Cell. 2016 Feb 11; 164(4):770-9. PMID: 26830879.
      View in: PubMed
    12. Coyle SM, Lim W. Mapping the functional versatility and fragility of Ras GTPase signaling circuits through in vitro network reconstitution. Elife. 2016; 5. PMID: 26765565.
      View in: PubMed
    13. Chen B, Hu J, Almeida R, Liu H, Balakrishnan S, Covill-Cooke C, Lim W, Huang B. Expanding the CRISPR imaging toolset with Staphylococcus aureus Cas9 for simultaneous imaging of multiple genomic loci. Nucleic Acids Res. 2016 May 5; 44(8):e75. PMID: 26740581.
      View in: PubMed
    14. Dominguez AA, Lim W, Qi LS. Beyond editing: repurposing CRISPR-Cas9 for precision genome regulation and interrogation. Nat Rev Mol Cell Biol. 2016 Jan; 17(1):5-15. PMID: 26670017.
      View in: PubMed
    15. Mitchell A, Wei P, Lim W. Oscillatory stress stimulation uncovers an Achilles' heel of the yeast MAPK signaling network. Science. 2015 Dec 11; 350(6266):1379-83. PMID: 26586187.
      View in: PubMed
    16. Wu CY, Roybal KT, Puchner EM, Onuffer J, Lim W. Remote control of therapeutic T cells through a small molecule-gated chimeric receptor. Science. 2015 Oct 16; 350(6258):aab4077. PMID: 26405231.
      View in: PubMed
    17. Wu CY, Rupp LJ, Roybal KT, Lim W. Synthetic biology approaches to engineer T cells. Curr Opin Immunol. 2015 Aug; 35:123-30. PMID: 26218616; PMCID: PMC4553068 [Available on 08/01/16].
    18. Martin H, Shales M, Fernandez-Piñar P, Wei P, Molina M, Fiedler D, Shokat KM, Beltrao P, Lim W, Krogan NJ. Differential genetic interactions of yeast stress response MAPK pathways. Mol Syst Biol. 2015 Apr; 11(4):800. PMID: 25888283; PMCID: PMC4422557.
    19. Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M, Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim W. Engineering complex synthetic transcriptional programs with CRISPR RNA scaffolds. Cell. 2015 Jan 15; 160(1-2):339-50. PMID: 25533786; PMCID: PMC4297522 [Available on 01/15/16].
    20. Ji W, Lee D, Wong E, Dadlani P, Dinh D, Huang V, Kearns K, Teng S, Chen S, Haliburton J, Heimberg G, Heineike B, Ramasubramanian A, Stevens T, Helmke KJ, Zepeda V, Qi LS, Lim W. Specific gene repression by CRISPRi system transferred through bacterial conjugation. ACS Synth Biol. 2014 Dec 19; 3(12):929-31. PMID: 25409531; PMCID: PMC4277763.
    21. Youk H, Lim W. Sending mixed messages for cell population control. Cell. 2014 Aug 28; 158(5):973-5. PMID: 25171399; PMCID: PMC4351990.
    22. Park JS, Rhau B, Hermann A, McNally KA, Zhou C, Gong D, Weiner OD, Conklin BR, Onuffer J, Lim W. Synthetic control of mammalian-cell motility by engineering chemotaxis to an orthogonal bioinert chemical signal. Proc Natl Acad Sci U S A. 2014 Apr 22; 111(16):5896-901. PMID: 24711398; PMCID: PMC4000811.
    23. Youk H, Lim W. Secreting and sensing the same molecule allows cells to achieve versatile social behaviors. Science. 2014 Feb 7; 343(6171):1242782. PMID: 24503857; PMCID: PMC4145839.
    24. Toettcher JE, Weiner OD, Lim W. Using optogenetics to interrogate the dynamic control of signal transmission by the Ras/Erk module. Cell. 2013 Dec 5; 155(6):1422-34. PMID: 24315106; PMCID: PMC3925772.
    25. Jagirdar K, Yin K, Harrison M, Lim W, Muscat GE, Sturm RA, Smith AG. The NR4A2 nuclear receptor is recruited to novel nuclear foci in response to UV irradiation and participates in nucleotide excision repair. PLoS One. 2013; 8(11):e78075. PMID: 24223135; PMCID: PMC3819332.
    26. Moser F, Horwitz A, Chen J, Lim W, Voigt CA. Genetic sensor for strong methylating compounds. ACS Synth Biol. 2013 Oct 18; 2(10):614-24. PMID: 24032656; PMCID: PMC3859139.
    27. Larson MH, Gilbert LA, Wang X, Lim W, Weissman JS, Qi LS. CRISPR interference (CRISPRi) for sequence-specific control of gene expression. Nat Protoc. 2013 Nov; 8(11):2180-96. PMID: 24136345; PMCID: PMC3922765.
    28. Dandekar SN, Park JS, Peng GE, Onuffer JJ, Lim W, Weiner OD. Actin dynamics rapidly reset chemoattractant receptor sensitivity following adaptation in neutrophils. Philos Trans R Soc Lond B Biol Sci. 2013; 368(1629):20130008. PMID: 24062580; PMCID: PMC3785960.
    29. Puchner EM, Walter JM, Kasper R, Huang B, Lim W. Counting molecules in single organelles with superresolution microscopy allows tracking of the endosome maturation trajectory. Proc Natl Acad Sci U S A. 2013 Oct 1; 110(40):16015-20. PMID: 24043832; PMCID: PMC3791776.
    30. Coyle SM, Flores J, Lim W. Exploitation of latent allostery enables the evolution of new modes of MAP kinase regulation. Cell. 2013 Aug 15; 154(4):875-87. PMID: 23953117; PMCID: PMC3787944.
    31. Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim W, Weissman JS, Qi LS. CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell. 2013 Jul 18; 154(2):442-51. PMID: 23849981; PMCID: PMC3770145.
    32. Lee S, Lim W, Thorn KS. Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS One. 2013; 8(7):e67902. PMID: 23844123; PMCID: PMC3699464.
    33. Hynes NE, Ingham PW, Lim W, Marshall CJ, Massagué J, Pawson T. Signalling change: signal transduction through the decades. Nat Rev Mol Cell Biol. 2013 Jun; 14(6):393-8. PMID: 23636498.
      View in: PubMed
    34. Fischbach MA, Bluestone JA, Lim W. Cell-based therapeutics: the next pillar of medicine. Sci Transl Med. 2013 Apr 3; 5(179):179ps7. PMID: 23552369; PMCID: PMC3772767.
    35. Lee CM, Gong S, Tang C, Lim W. Bridging cross-cultural gaps in scientific exchange through innovative team challenge workshops. Quant Biol. 2013 Mar; 1(1):3-8. PMID: 25339992.
      View in: PubMed
    36. Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim W. Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell. 2013 Feb 28; 152(5):1173-83. PMID: 23452860; PMCID: PMC3664290.
    37. Lim W, Lee CM, Tang C. Design principles of regulatory networks: searching for the molecular algorithms of the cell. Mol Cell. 2013 Jan 24; 49(2):202-12. PMID: 23352241; PMCID: PMC3664230.
    38. Lim W, Alvania R, Marshall WF. Cell biology 2.0. Trends Cell Biol. 2012 Dec; 22(12):611-2. PMID: 23142093.
      View in: PubMed
    39. Chau AH, Walter JM, Gerardin J, Tang C, Lim W. Designing synthetic regulatory networks capable of self-organizing cell polarization. Cell. 2012 Oct 12; 151(2):320-32. PMID: 23039994; PMCID: PMC3498761.
    40. Lim W. Wendell Lim: exploring the path not chosen. Interview by Caitlin Sedwick. J Cell Biol. 2012 Sep 17; 198(6):956-7. PMID: 22986491; PMCID: PMC3444780.
    41. Maiuri P, Terriac E, Paul-Gilloteaux P, Vignaud T, McNally K, Onuffer J, Thorn K, Nguyen PA, Georgoulia N, Soong D, Jayo A, Beil N, Beneke J, Lim JC, Sim CP, Chu YS, Jiménez-Dalmaroni A, Joanny JF, Thiery JP, Erfle H, Parsons M, Mitchison TJ, Lim W, Lennon-Duménil AM, Piel M, Théry M. The first World Cell Race. Curr Biol. 2012 Sep 11; 22(17):R673-5. PMID: 22974990; PMCID: PMC3770281.
    42. Wei P, Wong WW, Park JS, Corcoran EE, Peisajovich SG, Onuffer JJ, Weiss A, Lim W. Bacterial virulence proteins as tools to rewire kinase pathways in yeast and immune cells. Nature. 2012 Aug 16; 488(7411):384-8. PMID: 22820255; PMCID: PMC3422413.
    43. Zalatan JG, Coyle SM, Rajan S, Sidhu SS, Lim W. Conformational control of the Ste5 scaffold protein insulates against MAP kinase misactivation. Science. 2012 Sep 7; 337(6099):1218-22. PMID: 22878499; PMCID: PMC3631425.
    44. Beltrao P, Albanèse V, Kenner LR, Swaney DL, Burlingame A, Villén J, Lim W, Fraser JS, Frydman J, Krogan NJ. Systematic functional prioritization of protein posttranslational modifications. Cell. 2012 Jul 20; 150(2):413-25. PMID: 22817900; PMCID: PMC3404735.
    45. Thompson KE, Bashor CJ, Lim W, Keating AE. SYNZIP protein interaction toolbox: in vitro and in vivo specifications of heterospecific coiled-coil interaction domains. ACS Synth Biol. 2012 Apr 20; 1(4):118-29. PMID: 22558529.
      View in: PubMed
    46. Kapp GT, Liu S, Stein A, Wong DT, Reményi A, Yeh BJ, Fraser JS, Taunton J, Lim W, Kortemme T. Control of protein signaling using a computationally designed GTPase/GEF orthogonal pair. Proc Natl Acad Sci U S A. 2012 Apr 3; 109(14):5277-82. PMID: 22403064; PMCID: PMC3325720.
    47. Toettcher JE, Gong D, Lim W, Weiner OD. Light-based feedback for controlling intracellular signaling dynamics. Nat Methods. 2011; 8(10):837-9. PMID: 21909100; PMCID: PMC3184382.
    48. Won AP, Garbarino JE, Lim W. Recruitment interactions can override catalytic interactions in determining the functional identity of a protein kinase. Proc Natl Acad Sci U S A. 2011 Jun 14; 108(24):9809-14. PMID: 21628578; PMCID: PMC3116413.
    49. Good MC, Zalatan JG, Lim W. Scaffold proteins: hubs for controlling the flow of cellular information. Science. 2011 May 6; 332(6030):680-6. PMID: 21551057; PMCID: PMC3117218.
    50. Smith AG, Lim W, Pearen M, Muscat GE, Sturm RA. Regulation of NR4A nuclear receptor expression by oncogenic BRAF in melanoma cells. Pigment Cell Melanoma Res. 2011 Jun; 24(3):551-63. PMID: 21362156.
      View in: PubMed
    51. Toettcher JE, Gong D, Lim W, Weiner OD. Light control of plasma membrane recruitment using the Phy-PIF system. Methods Enzymol. 2011; 497:409-23. PMID: 21601096; PMCID: PMC3121701.
    52. Toettcher JE, Voigt CA, Weiner OD, Lim W. The promise of optogenetics in cell biology: interrogating molecular circuits in space and time. Nat Methods. 2011 Jan; 8(1):35-8. PMID: 21191370; PMCID: PMC3024327.
    53. Elowitz M, Lim W. Build life to understand it. Nature. 2010 Dec 16; 468(7326):889-90. PMID: 21164460; PMCID: PMC3068207.
    54. Lim W, Pawson T. Phosphotyrosine signaling: evolving a new cellular communication system. Cell. 2010 Sep 3; 142(5):661-7. PMID: 20813250; PMCID: PMC2950826.
    55. Ahmad KF, Lim W. The minimal autoinhibited unit of the guanine nucleotide exchange factor intersectin. PLoS One. 2010; 5(6):e11291. PMID: 20585582; PMCID: PMC2892021.
    56. Lim W. Designing customized cell signalling circuits. Nat Rev Mol Cell Biol. 2010 Jun; 11(6):393-403. PMID: 20485291; PMCID: PMC2975372.
    57. Peisajovich SG, Garbarino JE, Wei P, Lim W. Rapid diversification of cell signaling phenotypes by modular domain recombination. Science. 2010 Apr 16; 328(5976):368-72. PMID: 20395511; PMCID: PMC2975375.
    58. Mok J, Kim PM, Lam HY, Piccirillo S, Zhou X, Jeschke GR, Sheridan DL, Parker SA, Desai V, Jwa M, Cameroni E, Niu H, Good M, Remenyi A, Ma JL, Sheu YJ, Sassi HE, Sopko R, Chan CS, De Virgilio C, Hollingsworth NM, Lim W, Stern DF, Stillman B, Andrews BJ, Gerstein MB, Snyder M, Turk BE. Deciphering protein kinase specificity through large-scale analysis of yeast phosphorylation site motifs. Sci Signal. 2010; 3(109):ra12. PMID: 20159853; PMCID: PMC2846625.
    59. Bashor CJ, Horwitz AA, Peisajovich SG, Lim W. Rewiring cells: synthetic biology as a tool to interrogate the organizational principles of living systems. Annu Rev Biophys. 2010; 39:515-37. PMID: 20192780; PMCID: PMC2965450.
    60. Yadav SS, Yeh BJ, Craddock BP, Lim W, Miller WT. Reengineering the signaling properties of a Src family kinase. Biochemistry. 2009 Nov 24; 48(46):10956-62. PMID: 19827767; PMCID: PMC2847435.
    61. Rivera GM, Vasilescu D, Papayannopoulos V, Lim W, Mayer BJ. A reciprocal interdependence between Nck and PI(4,5)P(2) promotes localized N-WASp-mediated actin polymerization in living cells. Mol Cell. 2009 Nov 13; 36(3):525-35. PMID: 19917259; PMCID: PMC2793683.
    62. Levskaya A, Weiner OD, Lim W, Voigt CA. Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature. 2009 Oct 15; 461(7266):997-1001. PMID: 19749742; PMCID: PMC2989900.
    63. Ma W, Trusina A, El-Samad H, Lim W, Tang C. Defining network topologies that can achieve biochemical adaptation. Cell. 2009 Aug 21; 138(4):760-73. PMID: 19703401; PMCID: PMC3068210.
    64. Zeke A, Lukács M, Lim W, Reményi A. Scaffolds: interaction platforms for cellular signalling circuits. Trends Cell Biol. 2009 Aug; 19(8):364-74. PMID: 19651513; PMCID: PMC3073007.
    65. Tan CS, Pasculescu A, Lim W, Pawson T, Bader GD, Linding R. Positive selection of tyrosine loss in metazoan evolution. Science. 2009 Sep 25; 325(5948):1686-8. PMID: 19589966; PMCID: PMC3066034.
    66. Beltrao P, Trinidad JC, Fiedler D, Roguev A, Lim W, Shokat KM, Burlingame AL, Krogan NJ. Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species. PLoS Biol. 2009 Jun 16; 7(6):e1000134. PMID: 19547744; PMCID: PMC2691599.
    67. Good M, Tang G, Singleton J, Reményi A, Lim W. The Ste5 scaffold directs mating signaling by catalytically unlocking the Fus3 MAP kinase for activation. Cell. 2009 Mar 20; 136(6):1085-97. PMID: 19303851; PMCID: PMC2777755.
    68. Lim W. Frederic M Richards 1925-2009. Nat Struct Mol Biol. 2009 Mar; 16(3):230-2. PMID: 19259121.
      View in: PubMed
    69. Sallee NA, Rivera GM, Dueber JE, Vasilescu D, Mullins RD, Mayer BJ, Lim W. The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalency. Nature. 2008 Aug 21; 454(7207):1005-8. PMID: 18650806; PMCID: PMC2749708.
    70. Pincus D, Letunic I, Bork P, Lim W. Evolution of the phospho-tyrosine signaling machinery in premetazoan lineages. Proc Natl Acad Sci U S A. 2008 Jul 15; 105(28):9680-4. PMID: 18599463; PMCID: PMC2443182.
    71. Bashor CJ, Helman NC, Yan S, Lim W. Using engineered scaffold interactions to reshape MAP kinase pathway signaling dynamics. Science. 2008 Mar 14; 319(5869):1539-43. PMID: 18339942.
      View in: PubMed
    72. King N, Westbrook MJ, Young SL, Kuo A, Abedin M, Chapman J, Fairclough S, Hellsten U, Isogai Y, Letunic I, Marr M, Pincus D, Putnam N, Rokas A, Wright KJ, Zuzow R, Dirks W, Good M, Goodstein D, Lemons D, Li W, Lyons JB, Morris A, Nichols S, Richter DJ, Salamov A, Sequencing JG, Bork P, Lim W, Manning G, Miller WT, McGinnis W, Shapiro H, Tjian R, Grigoriev IV, Rokhsar D. The genome of the choanoflagellate Monosiga brevicollis and the origin of metazoans. Nature. 2008 Feb 14; 451(7180):783-8. PMID: 18273011; PMCID: PMC2562698.
    73. Lee PJ, Helman NC, Lim W, Hung PJ. A microfluidic system for dynamic yeast cell imaging. Biotechniques. 2008 Jan; 44(1):91-5. PMID: 18254385.
      View in: PubMed
    74. Yeh BJ, Lim W. Synthetic biology: lessons from the history of synthetic organic chemistry. Nat Chem Biol. 2007 Sep; 3(9):521-5. PMID: 17710092.
      View in: PubMed
    75. Yeh BJ, Rutigliano RJ, Deb A, Bar-Sagi D, Lim W. Rewiring cellular morphology pathways with synthetic guanine nucleotide exchange factors. Nature. 2007 May 31; 447(7144):596-600. PMID: 17515921.
      View in: PubMed
    76. Dueber JE, Mirsky EA, Lim W. Engineering synthetic signaling proteins with ultrasensitive input/output control. Nat Biotechnol. 2007 Jun; 25(6):660-2. PMID: 17515908.
      View in: PubMed
    77. Sallee NA, Yeh BJ, Lim W. Engineering modular protein interaction switches by sequence overlap. J Am Chem Soc. 2007 Apr 18; 129(15):4606-11. PMID: 17381089.
      View in: PubMed
    78. Peterson FC, Deng Q, Zettl M, Prehoda KE, Lim W, Way M, Volkman BF. Multiple WASP-interacting protein recognition motifs are required for a functional interaction with N-WASP. J Biol Chem. 2007 Mar 16; 282(11):8446-53. PMID: 17229736.
      View in: PubMed
    79. Hammond MC, Harris BZ, Lim W, Bartlett PA. Beta strand peptidomimetics as potent PDZ domain ligands. Chem Biol. 2006 Dec; 13(12):1247-51. PMID: 17185220.
      View in: PubMed
    80. Reményi A, Good MC, Lim W. Docking interactions in protein kinase and phosphatase networks. Curr Opin Struct Biol. 2006 Dec; 16(6):676-85. PMID: 17079133.
      View in: PubMed
    81. Bhattacharyya RP, Reményi A, Good MC, Bashor CJ, Falick AM, Lim W. The Ste5 scaffold allosterically modulates signaling output of the yeast mating pathway. Science. 2006 Feb 10; 311(5762):822-6. PMID: 16424299.
      View in: PubMed
    82. Bhattacharyya RP, Reményi A, Yeh BJ, Lim W. Domains, motifs, and scaffolds: the role of modular interactions in the evolution and wiring of cell signaling circuits. Annu Rev Biochem. 2006; 75:655-80. PMID: 16756506.
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    83. Reményi A, Good MC, Bhattacharyya RP, Lim W. The role of docking interactions in mediating signaling input, output, and discrimination in the yeast MAPK network. Mol Cell. 2005 Dec 22; 20(6):951-62. PMID: 16364919.
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    84. Petrosky KY, Ou HD, Löhr F, Dötsch V, Lim W. A general model for preferential hetero-oligomerization of LIN-2/7 domains: mechanism underlying directed assembly of supramolecular signaling complexes. J Biol Chem. 2005 Nov 18; 280(46):38528-36. PMID: 16147993.
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    85. Papayannopoulos V, Co C, Prehoda KE, Snapper S, Taunton J, Lim W. A polybasic motif allows N-WASP to act as a sensor of PIP(2) density. Mol Cell. 2005 Jan 21; 17(2):181-91. PMID: 15664188.
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    86. Dueber JE, Yeh BJ, Bhattacharyya RP, Lim W. Rewiring cell signaling: the logic and plasticity of eukaryotic protein circuitry. Curr Opin Struct Biol. 2004 Dec; 14(6):690-9. PMID: 15582393.
      View in: PubMed
    87. Zarrinpar A, Bhattacharyya RP, Nittler MP, Lim W. Sho1 and Pbs2 act as coscaffolds linking components in the yeast high osmolarity MAP kinase pathway. Mol Cell. 2004 Jun 18; 14(6):825-32. PMID: 15200959.
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    88. Zarrinpar A, Park SH, Lim W. Optimization of specificity in a cellular protein interaction network by negative selection. Nature. 2003 Dec 11; 426(6967):676-80. PMID: 14668868.
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    89. Dueber JE, Yeh BJ, Chak K, Lim W. Reprogramming control of an allosteric signaling switch through modular recombination. Science. 2003 Sep 26; 301(5641):1904-8. PMID: 14512628.
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    90. Carlton VE, Harris BZ, Puffenberger EG, Batta AK, Knisely AS, Robinson DL, Strauss KA, Shneider BL, Lim W, Salen G, Morton DH, Bull LN. Complex inheritance of familial hypercholanemia with associated mutations in TJP2 and BAAT. Nat Genet. 2003 May; 34(1):91-6. PMID: 12704386.
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    91. Zarrinpar A, Bhattacharyya RP, Lim W. The structure and function of proline recognition domains. Sci STKE. 2003 Apr 22; 2003(179):RE8. PMID: 12709533.
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    92. Harris BZ, Lau FW, Fujii N, Guy RK, Lim W. Role of electrostatic interactions in PDZ domain ligand recognition. Biochemistry. 2003 Mar 18; 42(10):2797-805. PMID: 12627945.
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    93. Park SH, Zarrinpar A, Lim W. Rewiring MAP kinase pathways using alternative scaffold assembly mechanisms. Science. 2003 Feb 14; 299(5609):1061-4. PMID: 12511654.
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    94. Volkman BF, Prehoda KE, Scott JA, Peterson FC, Lim W. Structure of the N-WASP EVH1 domain-WIP complex: insight into the molecular basis of Wiskott-Aldrich Syndrome. Cell. 2002 Nov 15; 111(4):565-76. PMID: 12437929.
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    95. Yu TW, Hao JC, Lim W, Tessier-Lavigne M, Bargmann CI. Shared receptors in axon guidance: SAX-3/Robo signals via UNC-34/Enabled and a Netrin-independent UNC-40/DCC function. Nat Neurosci. 2002 Nov; 5(11):1147-54. PMID: 12379860.
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    96. Harris BZ, Venkatasubrahmanyam S, Lim W. Coordinated folding and association of the LIN-2, -7 (L27) domain. An obligate heterodimerization involved in assembly of signaling and cell polarity complexes. J Biol Chem. 2002 Sep 20; 277(38):34902-8. PMID: 12110687.
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    97. Prehoda KE, Lim W. How signaling proteins integrate multiple inputs: a comparison of N-WASP and Cdk2. Curr Opin Cell Biol. 2002 Apr; 14(2):149-54. PMID: 11891112.
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    98. Lim W. The modular logic of signaling proteins: building allosteric switches from simple binding domains. Curr Opin Struct Biol. 2002 Feb; 12(1):61-8. PMID: 11839491.
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    99. McGee AW, Dakoji SR, Olsen O, Bredt DS, Lim W, Prehoda KE. Structure of the SH3-guanylate kinase module from PSD-95 suggests a mechanism for regulated assembly of MAGUK scaffolding proteins. Mol Cell. 2001 Dec; 8(6):1291-301. PMID: 11779504.
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    100. Harris BZ, Lim W. Mechanism and role of PDZ domains in signaling complex assembly. J Cell Sci. 2001 Sep; 114(Pt 18):3219-31. PMID: 11591811.
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    101. Prehoda KE, Lim W. The double life of PX domains. Nat Struct Biol. 2001 Jul; 8(7):570-2. PMID: 11427879.
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    102. Harris BZ, Hillier BJ, Lim W. Energetic determinants of internal motif recognition by PDZ domains. Biochemistry. 2001 May 22; 40(20):5921-30. PMID: 11352727.
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    103. Wang W, Lim W, Jakalian A, Wang J, Wang J, Luo R, Bayly CI, Kollman PA. An analysis of the interactions between the Sem-5 SH3 domain and its ligands using molecular dynamics, free energy calculations, and sequence analysis. J Am Chem Soc. 2001 May 2; 123(17):3986-94. PMID: 11457149.
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    104. Prehoda KE, Scott JA, Mullins RD, Lim W. Integration of multiple signals through cooperative regulation of the N-WASP-Arp2/3 complex. Science. 2000 Oct 27; 290(5492):801-6. PMID: 11052943.
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    105. Zarrinpar A, Lim W. Converging on proline: the mechanism of WW domain peptide recognition. Nat Struct Biol. 2000 Aug; 7(8):611-3. PMID: 10932238.
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    106. Nguyen JT, Porter M, Amoui M, Miller WT, Zuckermann RN, Lim W. Improving SH3 domain ligand selectivity using a non-natural scaffold. Chem Biol. 2000 Jul; 7(7):463-73. PMID: 10903934.
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    107. Christopherson KS, Hillier BJ, Lim W, Bredt DS. PSD-95 assembles a ternary complex with the N-methyl-D-aspartic acid receptor and a bivalent neuronal NO synthase PDZ domain. J Biol Chem. 1999 Sep 24; 274(39):27467-73. PMID: 10488080.
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    108. Prehoda KE, Lee DJ, Lim W. Structure of the enabled/VASP homology 1 domain-peptide complex: a key component in the spatial control of actin assembly. Cell. 1999 May 14; 97(4):471-80. PMID: 10338211.
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    109. Hillier BJ, Christopherson KS, Prehoda KE, Bredt DS, Lim W. Unexpected modes of PDZ domain scaffolding revealed by structure of nNOS-syntrophin complex. Science. 1999 Apr 30; 284(5415):812-5. PMID: 10221915.
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    110. Nguyen JT, Turck CW, Cohen FE, Zuckermann RN, Lim W. Exploiting the basis of proline recognition by SH3 and WW domains: design of N-substituted inhibitors. Science. 1998 Dec 11; 282(5396):2088-92. PMID: 9851931.
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    111. Nguyen JT, Lim W. How Src exercises self-restraint. Nat Struct Biol. 1997 Apr; 4(4):256-60. PMID: 9095189.
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    112. Lim W. Reading between the lines: SH3 recognition of an intact protein. Structure. 1996 Jun 15; 4(6):657-9. PMID: 8805558.
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    113. Lim W, Richards FM, Fox RO. Structural determinants of peptide-binding orientation and of sequence specificity in SH3 domains. Nature. 1994 Nov 24; 372(6504):375-9. PMID: 7802869.
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    114. Lim W, Fox RO, Richards FM. Stability and peptide binding affinity of an SH3 domain from the Caenorhabditis elegans signaling protein Sem-5. Protein Sci. 1994 Aug; 3(8):1261-6. PMID: 7987221; PMCID: PMC2142924.
    115. Lim W, Richards FM. Critical residues in an SH3 domain from Sem-5 suggest a mechanism for proline-rich peptide recognition. Nat Struct Biol. 1994 Apr; 1(4):221-5. PMID: 7656049.
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    116. Lim W, Hodel A, Sauer RT, Richards FM. The crystal structure of a mutant protein with altered but improved hydrophobic core packing. Proc Natl Acad Sci U S A. 1994 Jan 4; 91(1):423-7. PMID: 8278404; PMCID: PMC42960.
    117. Richards FM, Lim W. An analysis of packing in the protein folding problem. Q Rev Biophys. 1993 Nov; 26(4):423-98. PMID: 8058892.
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    118. Lim W, Farruggio DC, Sauer RT. Structural and energetic consequences of disruptive mutations in a protein core. Biochemistry. 1992 May 5; 31(17):4324-33. PMID: 1567879.
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    119. Lim W, Sauer RT. The role of internal packing interactions in determining the structure and stability of a protein. J Mol Biol. 1991 May 20; 219(2):359-76. PMID: 2038061.
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    120. Lim W, Sauer RT, Lander AD. Analysis of DNA-protein interactions by affinity coelectrophoresis. Methods Enzymol. 1991; 208:196-210. PMID: 1838134.
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    121. Reidhaar-Olson JF, Bowie JU, Breyer RM, Hu JC, Knight KL, Lim W, Mossing MC, Parsell DA, Shoemaker KR, Sauer RT. Random mutagenesis of protein sequences using oligonucleotide cassettes. Methods Enzymol. 1991; 208:564-86. PMID: 1779849.
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    122. Bowie JU, Reidhaar-Olson JF, Lim W, Sauer RT. Deciphering the message in protein sequences: tolerance to amino acid substitutions. Science. 1990 Mar 16; 247(4948):1306-10. PMID: 2315699.
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    123. Lim W, Sauer RT. Alternative packing arrangements in the hydrophobic core of lambda repressor. Nature. 1989 May 4; 339(6219):31-6. PMID: 2524006.
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