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Bruce Conklin, MD

Title(s)Professor, Medicine
SchoolSchool of Medicine
Phone415-734-2712
ORCID ORCID Icon0000-0003-1463-6061 Additional info
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    Collapse Biography 
    Collapse Education and Training
    University of California, Berkeley, CAA.B.1982Public Health
    Case Western Reserve U., Cleveland, OHM.D.1988Medicine
    Collapse Awards and Honors
    1990Medical Resident Research Award-NIDDK
    2003American Society for Clinical Investigation
    2008Scientific American 50 Award
    2011Fellow, California Academy of Sciences
    2016CWRU School of Medicine Distinguished Alumni Award

    Collapse Overview 
    Collapse Overview
    Therapeutic Approaches to Genetic Disease

    Genome Engineering
    The late Richard Feynman once said, “What I cannot create, I do not understand.” Human genetics began as observational science, but newly developed genome-engineering tools now allow us to directly test the cellular consequences of discrete genetic changes. We have developed efficient methods to edit one residue at a time in living human induced pluripotent stem (iPS) cells, resulting in “isogenic” lines of iPS cells. These isogenic lines form models that are now yielding phenotypes that are helping to explain the molecular basis of several human diseases. We are constructing collections of these isogenic lines that carry a range of disease mutations, from the most severe (rare) to the moderate (common) forms of cardiomyopathy.

    Precise Genome Editing for Human Therapy
    The rapid development of genome engineering, such as with the CRISPR system, now allows us to contemplate using these tools for human therapy in selective tissues. Using genome editing to “fix” a disease gene has great promise, because it could permanently correct the disease. However, tremendous challenges remain to ensure that only a specific part of the genome is edited, without causing “off-target” DNA damage that could lead to cancer. We focused on developing therapeutic editing for diseases of the heart and retina, because each tissue has unique challenges and opportunities. The heart has many newly described gene mutations that result in lethal heart failure (cardiomyopathy). Genome engineering can be used to cure cardiomyopathy mutations and validate targets for drug therapy; however, current engineering methods will need to be greatly enhanced before it can be used in vivo in the heart. In contrast, the retina has the advantage of a limited number of cells that can be directly targeted for therapy. These properties could potentially cure blindness caused by many gene mutations that currently have no therapy. We are developing proof-of-concept therapeutic editing methods in the retina that can be expanded to other tissues, such as the heart.

    Human Cardiac Disease Models
    We use iPS cells to model the genetic causes of human cardiomyopathy. Surprisingly, little is known about many genes associated with heart failure, from cardiomyopathy to abnormal heart rhythm, that results in “sudden death.” The heart provides an ideal system to determine the molecular basis of gene associations in human genetics. Until recently, modeling human gene variants in human cardiac tissue has been impossible. Human iPS cells now allow us to produce cardiovascular tissues that are identical, except for a single gene that has been altered by genome engineering. Our efforts have already been used to uncover multiple disease phenotypes and targets for drug therapy.

    CRISPR-Based Screens in Human Cardiac Disease Models
    Recently, we developed CRISPR-inhibition (CRISPRi) cell lines for highthroughput gene inactivation of thousands of different genes. CRISPRi screens can be used to identify the molecular basis of development, so that we can construct more mature human tissues and improved disease models. These studies will also allow us to identify drug targets that could be used for treating cardiomyopathy and other major diseases. We are currently focused on finding new pathways to enhance cardiac regeneration. In the future, other versions of the CRISPR system could be used to activate genes and control the epigenetic state of the genome.

    Future Directions
    We are developing new genome engineering methods in human iPS cells to identify therapeutic targets in cardiac disease. We are using these same tools to develop strategies for therapeutic genome editing. The combination of human iPS cells and genome editing provide unprecedented opportunities to explore new areas of biology and discover new therapies for disease.

    Selected Recent Publications
    1. Mandegar MA, Huebsch N, Frolov E, SHin E, Weissman JS, Qi LS, So P-L, Conklin BR. (2016) CRISPR interference efficiently induces gene knockdown and models disease in iPSCs. Cell Stem Cell. 18 1-13, April 7, 2016
    2. Huebsch N et al. (2015) Automated video-based analysis of contractility and calcium flux in human-induced pluripotent stem cell-derived cardiomyocytes cultured over different spatial scales. Tissue Eng. Part C Methods 21:467.
    3. Ma Z et al. (2015) Self-organizing human cardiac microchambers mediated by geometric confinement. Nat. Commun. 6:7413.
    4. Miyaoka Y et al. (2014) Isolation of single-base genome-edited human iPS cells without antibiotic selection. Nat. Methods 11:291.
    5. Spencer CI et al. (2014) Calcium transients closely reflect prolonged action potentials in iPSC models of inherited cardiac arrhythmia. Stem Cell Reports 3:269.

    Collapse Research 
    Collapse Research Activities and Funding
    Therapeutic genome editing to treat Best disease
    NIH/NEI R01EY028249Sep 30, 2017 - Jun 30, 2022
    Role: Principal Investigator
    Protein quality control, cardiomyopathy, cardiotoxicity and human isogenic iPSCs
    NIH/NHLBI R01HL135358Jul 15, 2017 - Jun 30, 2021
    Role: Principal Investigator
    Identifying Therapeutic Targets for RNA Splicing-Related Cardiomyopathy
    NIH/NHLBI R01HL130533Dec 14, 2015 - Nov 30, 2019
    Role: Principal Investigator
    Disease Specific Cardiac Tissue Models
    NIH/NHLBI R01HL108677Sep 5, 2011 - May 31, 2015
    Role: Co-Principal Investigator
    Structures of Protein Complexes Regulating Transcription in Enbryonic Stem Cells
    NIH/NIGMS U01GM094614Sep 30, 2010 - Jun 30, 2015
    Role: Co-Investigator
    Induced Pluripotent Stem Cells in the Understanding and Treatment of Heart Diseas
    NIH/NHLBI U01HL100406Sep 30, 2009 - Jun 30, 2016
    Role: Co-Principal Investigator
    The Epigenetic Landscape of Heart Development
    NIH/NHLBI U01HL098179Sep 30, 2009 - Aug 31, 2015
    Role: Co-Principal Investigator
    Induced pluripotent stem cells in the understanding and treatment of heart diseas
    NIH/NHLBI R03HL096254Dec 1, 2008 - May 31, 2009
    Role: Co-Principal Investigator
    Transcriptional Networks During Cardiac Differentiation
    NIH/NHLBI P01HL089707Jul 1, 2007 - Jul 31, 2018
    Role: Co-Investigator
    GenMAPP-CS, a dynamic resource pathway analysis
    NIH/NIGMS R01GM080223Aug 18, 2004 - Jul 31, 2012
    Role: Principal Investigator
    GenMAPP: A tool for pathway analysis of genomic data
    NIH/NHGRI R01HG003053Aug 18, 2004 - Oct 31, 2007
    Role: Principal Investigator
    COLLABORATIVE R01--ROLES OF G PROTEINS IN CARDIOMYOPATHY
    NIH/NHLBI R01HL061689Sep 30, 1998 - Aug 31, 2003
    Role: Principal Investigator
    Tissue Engineering with a Modular RASSL Toolbox
    NIH/NHLBI R01HL060664Jul 1, 1998 - Jun 30, 2013
    Role: Principal Investigator
    MUTANT G PROTEINS REVEAL SIGNALING PATHWAYS
    NIH/NHLBI K11HL002555Jan 1, 1991 - Dec 31, 1995
    Role: Principal Investigator

    Collapse ORNG Applications 
    Collapse Featured Publications
    Collapse Websites
    Collapse In The News
    Collapse Faculty Mentoring
    Collapse Featured Videos

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
    List All   |   Timeline
    1. Libby ARG, Briers D, Haghighi I, Joy DA, Conklin BR, Belta C, McDevitt TC. Automated Design of Pluripotent Stem Cell Self-Organization. Cell Syst. 2019 Nov 27; 9(5):483-495.e10. PMID: 31759947.
      View in: PubMed
    2. Keough KC, Lyalina S, Olvera MP, Whalen S, Conklin BR, Pollard KS. AlleleAnalyzer: a tool for personalized and allele-specific sgRNA design. Genome Biol. 2019 08 15; 20(1):167. PMID: 31416467.
      View in: PubMed
    3. Juan A Perez-Bermejo, Luke M Judge, Kenneth Wu, Christina L Jensen, Robyn M Kaake, Danielle Swaney, Po-Lin So, Nevan J Krogan, Bruce R Conklin. Abstract 934: Physical and Genetic Interaction Analyses in Human Pluripotent Stem Cell-Derived Cardiomyocytes to Study Protein Quality Control Disease in the Heart. Circulation Research. 2019 Aug 2; 125(Suppl_1).
      View in: Publisher Site
    4. Yoshinaga D, Baba S, Makiyama T, Shibata H, Hirata T, Akagi K, Matsuda K, Kohjitani H, Wuriyanghai Y, Umeda K, Yamamoto Y, Conklin BR, Horie M, Takita J, Heike T. Phenotype-Based High-Throughput Classification of Long QT Syndrome Subtypes Using Human Induced Pluripotent Stem Cells. Stem Cell Reports. 2019 Aug 13; 13(2):394-404. PMID: 31378668.
      View in: PubMed
    5. Song M, Yang X, Ren X, Maliskova L, Li B, Jones IR, Wang C, Jacob F, Wu K, Traglia M, Tam TW, Jamieson K, Lu SY, Ming GL, Li Y, Yao J, Weiss LA, Dixon JR, Judge LM, Conklin BR, Song H, Gan L, Shen Y. Mapping cis-regulatory chromatin contacts in neural cells links neuropsychiatric disorder risk variants to target genes. Nat Genet. 2019 Aug; 51(8):1252-1262. PMID: 31367015.
      View in: PubMed
    6. Claire Clelland, J. Iker Etchegaray, Lay Kodama, Faten Sayed, Aradhana Sachdev, Yaqiao Li, David Le, Lihong Zhan, Yungui Zhou, Duc Duong, Nicholas T. Seyfried, Thomas G. Kukar, Li Xuan Tan, Aparna Lakkaraju, Bruce Conklin, Li Gan. A NOVEL ROLE FOR THE FTD-CAUSING PROTEIN PROGRANULIN IN APOPTOSIS SIGNALING. Alzheimer's & Dementia. 2019 Jul 1; 15(7):p1509.
      View in: Publisher Site
    7. Wienert B, Wyman SK, Richardson CD, Yeh CD, Akcakaya P, Porritt MJ, Morlock M, Vu JT, Kazane KR, Watry HL, Judge LM, Conklin BR, Maresca M, Corn JE. Unbiased detection of CRISPR off-targets in vivo using DISCOVER-Seq. Science. 2019 04 19; 364(6437):286-289. PMID: 31000663.
      View in: PubMed
    8. Conklin BR. On the road to a gene drive in mammals. Nature. 2019 02; 566(7742):43-45. PMID: 30710124.
      View in: PubMed
    9. Eskildsen TV, Ayoubi S, Thomassen M, Burton M, Mandegar MA, Conklin BR, Jensen CH, Andersen DC, Sheikh SP. MESP1 knock-down in human iPSC attenuates early vascular progenitor cell differentiation after completed primitive streak specification. Dev Biol. 2019 01 01; 445(1):1-7. PMID: 30389344.
      View in: PubMed
    10. Libby AR, Joy DA, So PL, Mandegar MA, Muncie JM, Mendoza-Camacho FN, Weaver VM, Conklin BR, McDevitt TC. Spatiotemporal mosaic self-patterning of pluripotent stem cells using CRISPR interference. Elife. 2018 10 09; 7. PMID: 30298816.
      View in: PubMed
    11. Ma Z, Huebsch N, Koo S, Mandegar MA, Siemons B, Boggess S, Conklin BR, Grigoropoulos CP, Healy KE. Contractile deficits in engineered cardiac microtissues as a result of MYBPC3 deficiency and mechanical overload. Nat Biomed Eng. 2018 12; 2(12):955-967. PMID: 31015724.
      View in: PubMed
    12. Hoang P, Huebsch N, Bang SH, Siemons BA, Conklin BR, Healy KE, Ma Z, Jacquir S. Quantitatively characterizing drug-induced arrhythmic contractile motions of human stem cell-derived cardiomyocytes. Biotechnol Bioeng. 2018 08; 115(8):1958-1970. PMID: 29663322.
      View in: PubMed
    13. Plansky Hoang, Nathaniel Huebsch, Shin Hyuk Bang, Brian A. Siemons, Bruce R. Conklin, Kevin E. Healy, Zhen Ma, Sabir Jacquir. Quantitatively Characterizing Drug-Induced Arrhythmic Contractile Motions of Human Stem Cell-Derived Cardiomyocytes. Biotechnology and Bioengineering. 2018 Apr 12.
      View in: Publisher Site
    14. Hoang P, Wang J, Conklin BR, Healy KE, Ma Z. Generation of spatial-patterned early-developing cardiac organoids using human pluripotent stem cells. Nat Protoc. 2018 04; 13(4):723-737. PMID: 29543795.
      View in: PubMed
    15. Miyaoka Y, Mayerl SJ, Chan AH, Conklin BR. Detection and Quantification of HDR and NHEJ Induced by Genome Editing at Endogenous Gene Loci Using Droplet Digital PCR. Methods Mol Biol. 2018; 1768:349-362. PMID: 29717453.
      View in: PubMed
    16. Miller Huang, Tina Zheng, Jeffrey Guo, Colin Sperring, Matthew Miller, Lauren McHenry, Qiqi Zhen, Branden Moriarity, Marianne Bronner, Bruce Conklin, David Largaespada, John Maris, Katherine Matthay, William Weiss. TMOD-09. HUMAN PLURIPOTENT STEM CELL-BASED MODELS OF NEUROBLASTOMA. Neuro-Oncology. 2017 Nov 6; 19(suppl_6):vi256-vi256.
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    17. Judge LM, Perez-Bermejo JA, Truong A, Ribeiro AJ, Yoo JC, Jensen CL, Mandegar MA, Huebsch N, Kaake RM, So PL, Srivastava D, Pruitt BL, Krogan NJ, Conklin BR. A BAG3 chaperone complex maintains cardiomyocyte function during proteotoxic stress. JCI Insight. 2017 Jul 20; 2(14). PMID: 28724793.
      View in: PubMed
    18. Ribeiro AJS, Schwab O, Mandegar MA, Ang YS, Conklin BR, Srivastava D, Pruitt BL. Multi-Imaging Method to Assay the Contractile Mechanical Output of Micropatterned Human iPSC-Derived Cardiac Myocytes. Circ Res. 2017 May 12; 120(10):1572-1583. PMID: 28400398.
      View in: PubMed
    19. Liu SJ, Horlbeck MA, Cho SW, Birk HS, Malatesta M, He D, Attenello FJ, Villalta JE, Cho MY, Chen Y, Mandegar MA, Olvera MP, Gilbert LA, Conklin BR, Chang HY, Weissman JS, Lim DA. CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells. Science. 2017 01 06; 355(6320). PMID: 27980086.
      View in: PubMed
    20. Workman MJ, Mahe MM, Trisno S, Poling HM, Watson CL, Sundaram N, Chang CF, Schiesser J, Aubert P, Stanley EG, Elefanty AG, Miyaoka Y, Mandegar MA, Conklin BR, Neunlist M, Brugmann SA, Helmrath MA, Wells JM. Engineered human pluripotent-stem-cell-derived intestinal tissues with a functional enteric nervous system. Nat Med. 2017 01; 23(1):49-59. PMID: 27869805.
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    21. Siyuan Liu, Max Horlbeck, Seung Woo Cho, Harjus Birk, Martina Malatesta, Daniel He, Jacqueline Villalta, Min Cho, Yuwen Chen, Mohammad Mandegar, Michael Olvera, Bruce Conklin, Howard Chang, Jonathan Weissman, Daniel Lim. GENT-49. SYSTEMATIC IDENTIFICATION OF ESSENTIAL LONG NON-CODING RNA GENES IN GLIOBLASTOMA. Neuro-Oncology. 2016 Nov 1; 18(suppl_6):vi84-vi85.
      View in: Publisher Site
    22. Hayashi Y, Hsiao EC, Sami S, Lancero M, Schlieve CR, Nguyen T, Yano K, Nagahashi A, Ikeya M, Matsumoto Y, Nishimura K, Fukuda A, Hisatake K, Tomoda K, Asaka I, Toguchida J, Conklin BR, Yamanaka S. BMP-SMAD-ID promotes reprogramming to pluripotency by inhibiting p16/INK4A-dependent senescence. Proc Natl Acad Sci U S A. 2016 11 15; 113(46):13057-13062. PMID: 27794120.
      View in: PubMed
    23. P. Loskill, A. Mathur, B.R. Conklin, A. Stahl, L.P. Lee, K.E. Healy. Organs-on-a-chip – Microphysiological platforms as in vitro models of cardiac and adipose tissue. Toxicology Letters. 2016 Sep 1; 258:s153.
      View in: Publisher Site
    24. Miyaoka Y, Chan AH, Conklin BR. Detecting Single-Nucleotide Substitutions Induced by Genome Editing. Cold Spring Harb Protoc. 2016 08 01; 2016(8). PMID: 27250209.
      View in: PubMed
    25. Miyaoka Y, Chan AH, Conklin BR. Using Digital Polymerase Chain Reaction to Detect Single-Nucleotide Substitutions Induced by Genome Editing. Cold Spring Harb Protoc. 2016 08 01; 2016(8). PMID: 27250210.
      View in: PubMed
    26. Nguyen DP, Miyaoka Y, Gilbert LA, Mayerl SJ, Lee BH, Weissman JS, Conklin BR, Wells JA. Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity. Nat Commun. 2016 07 01; 7:12009. PMID: 27363581.
      View in: PubMed
    27. Huebsch N, Loskill P, Deveshwar N, Spencer CI, Judge LM, Mandegar MA, Fox CB, Mohamed TM, Ma Z, Mathur A, Sheehan AM, Truong A, Saxton M, Yoo J, Srivastava D, Desai TA, So PL, Healy KE, Conklin BR. Miniaturized iPS-Cell-Derived Cardiac Muscles for Physiologically Relevant Drug Response Analyses. Sci Rep. 2016 Apr 20; 6:24726. PMID: 27095412.
      View in: PubMed
    28. Miyaoka Y, Berman JR, Cooper SB, Mayerl SJ, Chan AH, Zhang B, Karlin-Neumann GA, Conklin BR. Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing. Sci Rep. 2016 Mar 31; 6:23549. PMID: 27030102.
      View in: PubMed
    29. Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, Weissman JS, Qi LS, So PL, Conklin BR. CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs. Cell Stem Cell. 2016 Apr 07; 18(4):541-53. PMID: 26971820.
      View in: PubMed
    30. Huang M, Miller ML, McHenry LK, Zheng T, Zhen Q, Ilkhanizadeh S, Conklin BR, Bronner ME, Weiss WA. Generating trunk neural crest from human pluripotent stem cells. Sci Rep. 2016 Jan 27; 6:19727. PMID: 26812940.
      View in: PubMed
    31. Kime C, Mandegar MA, Srivastava D, Yamanaka S, Conklin BR, Rand TA. Efficient CRISPR/Cas9-Based Genome Engineering in Human Pluripotent Stem Cells. Curr Protoc Hum Genet. 2016 Jan 01; 88:21.4.1-21.4.23. PMID: 26724721.
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    32. Ma Z, Wang J, Loskill P, Huebsch N, Koo S, Svedlund FL, Marks NC, Hua EW, Grigoropoulos CP, Conklin BR, Healy KE. Self-organizing human cardiac microchambers mediated by geometric confinement. Nat Commun. 2015 Jul 14; 6:7413. PMID: 26172574.
      View in: PubMed
    33. Nobuta H, Cilio MR, Danhaive O, Tsai HH, Tupal S, Chang SM, Murnen A, Kreitzer F, Bravo V, Czeisler C, Gokozan HN, Gygli P, Bush S, Weese-Mayer DE, Conklin B, Yee SP, Huang EJ, Gray PA, Rowitch D, Otero JJ. Dysregulation of locus coeruleus development in congenital central hypoventilation syndrome. Acta Neuropathol. 2015 Aug; 130(2):171-83. PMID: 25975378.
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    34. Wang L, Hsiao EC, Lieu S, Scott M, O'Carroll D, Urrutia A, Conklin BR, Colnot C, Nissenson RA. Loss of Gi G-Protein-Coupled Receptor Signaling in Osteoblasts Accelerates Bone Fracture Healing. J Bone Miner Res. 2015 Oct; 30(10):1896-904. PMID: 25917236.
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    35. Hayashi Y, Caboni L, Das D, Yumoto F, Clayton T, Deller MC, Nguyen P, Farr CL, Chiu HJ, Miller MD, Elsliger MA, Deacon AM, Godzik A, Lesley SA, Tomoda K, Conklin BR, Wilson IA, Yamanaka S, Fletterick RJ. Structure-based discovery of NANOG variant with enhanced properties to promote self-renewal and reprogramming of pluripotent stem cells. Proc Natl Acad Sci U S A. 2015 Apr 14; 112(15):4666-71. PMID: 25825768.
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    36. Maddah M, Heidmann JD, Mandegar MA, Walker CD, Bolouki S, Conklin BR, Loewke KE. A non-invasive platform for functional characterization of stem-cell-derived cardiomyocytes with applications in cardiotoxicity testing. Stem Cell Reports. 2015 Apr 14; 4(4):621-31. PMID: 25801505.
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    37. Mathur A, Loskill P, Shao K, Huebsch N, Hong S, Marcus SG, Marks N, Mandegar M, Conklin BR, Lee LP, Healy KE. Human iPSC-based cardiac microphysiological system for drug screening applications. Sci Rep. 2015 Mar 09; 5:8883. PMID: 25748532.
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    38. Wattanachanya L, Wang L, Millard SM, Lu WD, O'Carroll D, Hsiao EC, Conklin BR, Nissenson RA. Assessing the osteoblast transcriptome in a model of enhanced bone formation due to constitutive Gs-G protein signaling in osteoblasts. Exp Cell Res. 2015 May 01; 333(2):289-302. PMID: 25704759.
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    39. Berger M, Scheel DW, Macias H, Miyatsuka T, Kim H, Hoang P, Ku GM, Honig G, Liou A, Tang Y, Regard JB, Sharifnia P, Yu L, Wang J, Coughlin SR, Conklin BR, Deneris ES, Tecott LH, German MS. Gai/o-coupled receptor signaling restricts pancreatic ß-cell expansion. Proc Natl Acad Sci U S A. 2015 Mar 03; 112(9):2888-93. PMID: 25695968.
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    40. Orr AG, Hsiao EC, Wang MM, Ho K, Kim DH, Wang X, Guo W, Kang J, Yu GQ, Adame A, Devidze N, Dubal DB, Masliah E, Conklin BR, Mucke L. Astrocytic adenosine receptor A2A and Gs-coupled signaling regulate memory. Nat Neurosci. 2015 Mar; 18(3):423-34. PMID: 25622143.
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    41. Huebsch N, Loskill P, Mandegar MA, Marks NC, Sheehan AS, Ma Z, Mathur A, Nguyen TN, Yoo JC, Judge LM, Spencer CI, Chukka AC, Russell CR, So PL, Conklin BR, Healy KE. Automated Video-Based Analysis of Contractility and Calcium Flux in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Cultured over Different Spatial Scales. Tissue Eng Part C Methods. 2015 May; 21(5):467-79. PMID: 25333967.
      View in: PubMed
    42. Christopher M. Heylman, Rupsa Datta, Bruce R. Conklin, Steven C. George, Enrico Gratton. Classifying the Electrophysiological Effects of Chronotropic Drugs on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes using Voltage Sensitive Dyes and Supervised Machine Learning. Biophysical Journal. 2015 Jan 1; 108(2):110a.
      View in: Publisher Site
    43. Spencer CI, Baba S, Nakamura K, Hua EA, Sears MA, Fu CC, Zhang J, Balijepalli S, Tomoda K, Hayashi Y, Lizarraga P, Wojciak J, Scheinman MM, Aalto-Setälä K, Makielski JC, January CT, Healy KE, Kamp TJ, Yamanaka S, Conklin BR. Calcium transients closely reflect prolonged action potentials in iPSC models of inherited cardiac arrhythmia. Stem Cell Reports. 2014 Aug 12; 3(2):269-81. PMID: 25254341.
      View in: PubMed
    44. Park JS, Rhau B, Hermann A, McNally KA, Zhou C, Gong D, Weiner OD, Conklin BR, Onuffer J, Lim WA. Synthetic control of mammalian-cell motility by engineering chemotaxis to an orthogonal bioinert chemical signal. Proc Natl Acad Sci U S A. 2014 Apr 22; 111(16):5896-901. PMID: 24711398.
      View in: PubMed
    45. Miyaoka Y, Chan AH, Judge LM, Yoo J, Huang M, Nguyen TD, Lizarraga PP, So PL, Conklin BR. Isolation of single-base genome-edited human iPS cells without antibiotic selection. Nat Methods. 2014 Mar; 11(3):291-3. PMID: 24509632.
      View in: PubMed
    46. Mathur A, Loskill P, Hong S, Lee J, Marcus SG, Dumont L, Conklin BR, Willenbring H, Lee LP, Healy KE. Human induced pluripotent stem cell-based microphysiological tissue models of myocardium and liver for drug development. Stem Cell Res Ther. 2013; 4 Suppl 1:S14. PMID: 24565415.
      View in: PubMed
    47. Matsumoto Y, Hayashi Y, Schlieve CR, Ikeya M, Kim H, Nguyen TD, Sami S, Baba S, Barruet E, Nasu A, Asaka I, Otsuka T, Yamanaka S, Conklin BR, Toguchida J, Hsiao EC. Induced pluripotent stem cells from patients with human fibrodysplasia ossificans progressiva show increased mineralization and cartilage formation. Orphanet J Rare Dis. 2013 Dec 09; 8:190. PMID: 24321451.
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    48. Ma Z, Koo S, Finnegan MA, Loskill P, Huebsch N, Marks NC, Conklin BR, Grigoropoulos CP, Healy KE. Three-dimensional filamentous human diseased cardiac tissue model. Biomaterials. 2014 Feb; 35(5):1367-77. PMID: 24268663.
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    49. Taglieri DM, Johnson KR, Burmeister BT, Monasky MM, Spindler MJ, DeSantiago J, Banach K, Conklin BR, Carnegie GK. The C-terminus of the long AKAP13 isoform (AKAP-Lbc) is critical for development of compensatory cardiac hypertrophy. J Mol Cell Cardiol. 2014 Jan; 66:27-40. PMID: 24161911.
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    50. Conklin BR. Sculpting genomes with a hammer and chisel. Nat Methods. 2013 Sep; 10(9):839-40. PMID: 23985729.
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    51. Kreitzer FR, Salomonis N, Sheehan A, Huang M, Park JS, Spindler MJ, Lizarraga P, Weiss WA, So PL, Conklin BR. A robust method to derive functional neural crest cells from human pluripotent stem cells. Am J Stem Cells. 2013; 2(2):119-31. PMID: 23862100.
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    52. Spindler MJ, Burmeister BT, Huang Y, Hsiao EC, Salomonis N, Scott MJ, Srivastava D, Carnegie GK, Conklin BR. AKAP13 Rho-GEF and PKD-binding domain deficient mice develop normally but have an abnormal response to ß-adrenergic-induced cardiac hypertrophy. PLoS One. 2013; 8(4):e62705. PMID: 23658642.
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    53. Kumar A, Möcklinghoff S, Yumoto F, Jaroszewski L, Farr CL, Grzechnik A, Nguyen P, Weichenberger CX, Chiu HJ, Klock HE, Elsliger MA, Deacon AM, Godzik A, Lesley SA, Conklin BR, Fletterick RJ, Wilson IA. Structure of a novel winged-helix like domain from human NFRKB protein. PLoS One. 2012; 7(9):e43761. PMID: 22984442.
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    54. Tomoda K, Takahashi K, Leung K, Okada A, Narita M, Yamada NA, Eilertson KE, Tsang P, Baba S, White MP, Sami S, Srivastava D, Conklin BR, Panning B, Yamanaka S. Derivation conditions impact X-inactivation status in female human induced pluripotent stem cells. Cell Stem Cell. 2012 Jul 06; 11(1):91-9. PMID: 22770243.
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    55. Zambon AC, Gaj S, Ho I, Hanspers K, Vranizan K, Evelo CT, Conklin BR, Pico AR, Salomonis N. GO-Elite: a flexible solution for pathway and ontology over-representation. Bioinformatics. 2012 Aug 15; 28(16):2209-10. PMID: 22743224.
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    56. Kelder T, van Iersel MP, Hanspers K, Kutmon M, Conklin BR, Evelo CT, Pico AR. WikiPathways: building research communities on biological pathways. Nucleic Acids Res. 2012 Jan; 40(Database issue):D1301-7. PMID: 22096230.
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    57. Lahti AL, Kujala VJ, Chapman H, Koivisto AP, Pekkanen-Mattila M, Kerkelä E, Hyttinen J, Kontula K, Swan H, Conklin BR, Yamanaka S, Silvennoinen O, Aalto-Setälä K. Model for long QT syndrome type 2 using human iPS cells demonstrates arrhythmogenic characteristics in cell culture. Dis Model Mech. 2012 Mar; 5(2):220-30. PMID: 22052944.
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    58. Kazakia GJ, Speer D, Shanbhag S, Majumdar S, Conklin BR, Nissenson RA, Hsiao EC. Mineral composition is altered by osteoblast expression of an engineered G(s)-coupled receptor. Calcif Tissue Int. 2011 Jul; 89(1):10-20. PMID: 21526395.
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    60. Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR. Constitutive Gs activation using a single-construct tetracycline-inducible expression system in embryonic stem cells and mice. Stem Cell Res Ther. 2011 Mar 04; 2(2):11. PMID: 21375737.
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    62. Salomonis N, Conklin BR. Stem cell pluripotency: alternative modes of transcription regulation. Cell Cycle. 2010 Aug 15; 9(16):3133-4. PMID: 20814242.
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    64. Emig D, Salomonis N, Baumbach J, Lengauer T, Conklin BR, Albrecht M. AltAnalyze and DomainGraph: analyzing and visualizing exon expression data. Nucleic Acids Res. 2010 Jul; 38(Web Server issue):W755-62. PMID: 20513647.
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    65. Salomonis N, Schlieve CR, Pereira L, Wahlquist C, Colas A, Zambon AC, Vranizan K, Spindler MJ, Pico AR, Cline MS, Clark TA, Williams A, Blume JE, Samal E, Mercola M, Merrill BJ, Conklin BR. Alternative splicing regulates mouse embryonic stem cell pluripotency and differentiation. Proc Natl Acad Sci U S A. 2010 Jun 08; 107(23):10514-9. PMID: 20498046.
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    66. Hsiao EC, Boudignon BM, Halloran BP, Nissenson RA, Conklin BR. Gs G protein-coupled receptor signaling in osteoblasts elicits age-dependent effects on bone formation. J Bone Miner Res. 2010 Mar; 25(3):584-93. PMID: 20200944.
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    67. Hsiao EC, Millard SM, Louie A, Huang Y, Conklin BR, Nissenson RA. Ligand-mediated activation of an engineered gs g protein-coupled receptor in osteoblasts increases trabecular bone formation. Mol Endocrinol. 2010 Mar; 24(3):621-31. PMID: 20150184.
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    68. Lo B, Parham L, Alvarez-Buylla A, Cedars M, Conklin B, Fisher S, Gates E, Giudice L, Halme DG, Hershon W, Kriegstein A, Kwok PY, Wagner R. Cloning mice and men: prohibiting the use of iPS cells for human reproductive cloning. Cell Stem Cell. 2010 Jan 08; 6(1):16-20. PMID: 20085739.
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    69. van Iersel MP, Pico AR, Kelder T, Gao J, Ho I, Hanspers K, Conklin BR, Evelo CT. The BridgeDb framework: standardized access to gene, protein and metabolite identifier mapping services. BMC Bioinformatics. 2010 Jan 04; 11:5. PMID: 20047655.
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    70. Nakamura K, Salomonis N, Tomoda K, Yamanaka S, Conklin BR. G(i)-coupled GPCR signaling controls the formation and organization of human pluripotent colonies. PLoS One. 2009 Nov 10; 4(11):e7780. PMID: 19936228.
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    71. Salomonis N, Nelson B, Vranizan K, Pico AR, Hanspers K, Kuchinsky A, Ta L, Mercola M, Conklin BR. Alternative splicing in the differentiation of human embryonic stem cells into cardiac precursors. PLoS Comput Biol. 2009 Nov; 5(11):e1000553. PMID: 19893621.
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    72. Conklin BR. Journal club. A geneticist wonders why we need to sleep. Nature. 2009 Oct 01; 461(7264):573. PMID: 19794457.
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    73. Lo B, Conklin BR. Consent: criteria should be drawn up for tissue donors. Nature. 2009 Oct 01; 461(7264):593. PMID: 19794473.
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    74. Kelder T, Pico AR, Hanspers K, van Iersel MP, Evelo C, Conklin BR. Mining biological pathways using WikiPathways web services. PLoS One. 2009 Jul 30; 4(7):e6447. PMID: 19649250.
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    75. Neumann SA, Tingley WG, Conklin BR, Shrader CJ, Peet E, Muldoon MF, Jennings JR, Ferrell RE, Manuck SB. AKAP10 (I646V) functional polymorphism predicts heart rate and heart rate variability in apparently healthy, middle-aged European-Americans. Psychophysiology. 2009 May; 46(3):466-72. PMID: 19496216.
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    76. S. Millard, A. Louie, C. Manalac, W. Lu, N. Cotte, T.J. Wronski, B. Conklin, R.A. Nissenson. Endogenous Gi signaling in osteoblasts participates in sexually dimorphic regulation of cancellous bone structure. Bone. 2009 May 1; 44:s153.
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    77. Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot JG, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R, Calzolari D, Terskikh A, McCulloch AD, Price JH, Conklin BR, Chen HS, Mercola M. Lentiviral vectors and protocols for creation of stable hESC lines for fluorescent tracking and drug resistance selection of cardiomyocytes. PLoS One. 2009; 4(4):e5046. PMID: 19352491.
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    78. Aalto-Setälä K, Conklin BR, Lo B. Obtaining consent for future research with induced pluripotent cells: opportunities and challenges. PLoS Biol. 2009 Feb 24; 7(2):e42. PMID: 19243222.
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    79. Pico AR, Smirnov IV, Chang JS, Yeh RF, Wiemels JL, Wiencke JK, Tihan T, Conklin BR, Wrensch M. SNPLogic: an interactive single nucleotide polymorphism selection, annotation, and prioritization system. Nucleic Acids Res. 2009 Jan; 37(Database issue):D803-9. PMID: 18984625.
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    80. van Iersel MP, Kelder T, Pico AR, Hanspers K, Coort S, Conklin BR, Evelo C. Presenting and exploring biological pathways with PathVisio. BMC Bioinformatics. 2008 Sep 25; 9:399. PMID: 18817533.
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    81. Conklin BR, Hsiao EC, Claeysen S, Dumuis A, Srinivasan S, Forsayeth JR, Guettier JM, Chang WC, Pei Y, McCarthy KD, Nissenson RA, Wess J, Bockaert J, Roth BL. Engineering GPCR signaling pathways with RASSLs. Nat Methods. 2008 Aug; 5(8):673-8. PMID: 18668035.
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    83. Hsiao EC, Yoshinaga Y, Nguyen TD, Musone SL, Kim JE, Swinton P, Espineda I, Manalac C, deJong PJ, Conklin BR. Marking embryonic stem cells with a 2A self-cleaving peptide: a NKX2-5 emerald GFP BAC reporter. PLoS One. 2008 Jul 02; 3(7):e2532. PMID: 18596956.
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    84. Kristina Hanspers, Alexander R. Pico, Nathan Salomonis, Alexander C. Zambon, Karen Vranizan, Steven C. Lawlor, Kam D. Dahlquist, Scott W. Doniger, Bruce R. Conklin. An Introduction to Pathway Analysis with GenMAPP. NCI Nature Pathway Interaction Database. 2008 Apr 8.
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    85. Ivey KN, Muth A, Arnold J, King FW, Yeh RF, Fish JE, Hsiao EC, Schwartz RJ, Conklin BR, Bernstein HS, Srivastava D. MicroRNA regulation of cell lineages in mouse and human embryonic stem cells. Cell Stem Cell. 2008 Mar 06; 2(3):219-29. PMID: 18371447.
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    86. Hsiao EC, Boudignon BM, Chang WC, Bencsik M, Peng J, Nguyen TD, Manalac C, Halloran BP, Conklin BR, Nissenson RA. Osteoblast expression of an engineered Gs-coupled receptor dramatically increases bone mass. Proc Natl Acad Sci U S A. 2008 Jan 29; 105(4):1209-14. PMID: 18212126.
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    87. Chang WC, Ng JK, Nguyen T, Pellissier L, Claeysen S, Hsiao EC, Conklin BR. Modifying ligand-induced and constitutive signaling of the human 5-HT4 receptor. PLoS One. 2007 Dec 19; 2(12):e1317. PMID: 18338032.
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    88. Peng J, Bencsik M, Louie A, Lu W, Millard S, Nguyen P, Burghardt A, Majumdar S, Wronski TJ, Halloran B, Conklin BR, Nissenson RA. Conditional expression of a Gi-coupled receptor in osteoblasts results in trabecular osteopenia. Endocrinology. 2008 Mar; 149(3):1329-37. PMID: 18048501.
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    89. Zhang L, Zambon AC, Vranizan K, Pothula K, Conklin BR, Insel PA. Gene expression signatures of cAMP/protein kinase A (PKA)-promoted, mitochondrial-dependent apoptosis. Comparative analysis of wild-type and cAMP-deathless S49 lymphoma cells. J Biol Chem. 2008 Feb 15; 283(7):4304-13. PMID: 18048352.
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    90. Srinivasan S, Santiago P, Lubrano C, Vaisse C, Conklin BR. Engineering the melanocortin-4 receptor to control constitutive and ligand-mediated G(S) signaling in vivo. PLoS One. 2007 Aug 01; 2(7):e668. PMID: 17668051.
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    91. Nord AS, Vranizan K, Tingley W, Zambon AC, Hanspers K, Fong LG, Hu Y, Bacchetti P, Ferrin TE, Babbitt PC, Doniger SW, Skarnes WC, Young SG, Conklin BR. Modeling insertional mutagenesis using gene length and expression in murine embryonic stem cells. PLoS One. 2007 Jul 18; 2(7):e617. PMID: 17637833.
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    92. Salomonis N, Hanspers K, Zambon AC, Vranizan K, Lawlor SC, Dahlquist KD, Doniger SW, Stuart J, Conklin BR, Pico AR. GenMAPP 2: new features and resources for pathway analysis. BMC Bioinformatics. 2007 Jun 24; 8:217. PMID: 17588266.
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    93. Kwon C, Arnold J, Hsiao EC, Taketo MM, Conklin BR, Srivastava D. Canonical Wnt signaling is a positive regulator of mammalian cardiac progenitors. Proc Natl Acad Sci U S A. 2007 Jun 26; 104(26):10894-9. PMID: 17576928.
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    94. Kim W, Ng JK, Kunitake ME, Conklin BR, Yang P. Interfacing silicon nanowires with mammalian cells. J Am Chem Soc. 2007 Jun 13; 129(23):7228-9. PMID: 17516647.
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    95. Tingley WG, Pawlikowska L, Zaroff JG, Kim T, Nguyen T, Young SG, Vranizan K, Kwok PY, Whooley MA, Conklin BR. Gene-trapped mouse embryonic stem cell-derived cardiac myocytes and human genetics implicate AKAP10 in heart rhythm regulation. Proc Natl Acad Sci U S A. 2007 May 15; 104(20):8461-6. PMID: 17485678.
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    96. Conklin BR. New tools to build synthetic hormonal pathways. Proc Natl Acad Sci U S A. 2007 Mar 20; 104(12):4777-8. PMID: 17360323.
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    97. Sweger EJ, Casper KB, Scearce-Levie K, Conklin BR, McCarthy KD. Development of hydrocephalus in mice expressing the G(i)-coupled GPCR Ro1 RASSL receptor in astrocytes. J Neurosci. 2007 Feb 28; 27(9):2309-17. PMID: 17329428.
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    98. Cline MS, Smoot M, Cerami E, Kuchinsky A, Landys N, Workman C, Christmas R, Avila-Campilo I, Creech M, Gross B, Hanspers K, Isserlin R, Kelley R, Killcoyne S, Lotia S, Maere S, Morris J, Ono K, Pavlovic V, Pico AR, Vailaya A, Wang PL, Adler A, Conklin BR, Hood L, Kuiper M, Sander C, Schmulevich I, Schwikowski B, Warner GJ, Ideker T, Bader GD. Integration of biological networks and gene expression data using Cytoscape. Nat Protoc. 2007; 2(10):2366-82. PMID: 17947979.
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    99. Nicholls RE, Zhang XL, Bailey CP, Conklin BR, Kandel ER, Stanton PK. mGluR2 acts through inhibitory Galpha subunits to regulate transmission and long-term plasticity at hippocampal mossy fiber-CA3 synapses. Proc Natl Acad Sci U S A. 2006 Apr 18; 103(16):6380-5. PMID: 16606834.
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    100. Nord AS, Chang PJ, Conklin BR, Cox AV, Harper CA, Hicks GG, Huang CC, Johns SJ, Kawamoto M, Liu S, Meng EC, Morris JH, Rossant J, Ruiz P, Skarnes WC, Soriano P, Stanford WL, Stryke D, von Melchner H, Wurst W, Yamamura K, Young SG, Babbitt PC, Ferrin TE. The International Gene Trap Consortium Website: a portal to all publicly available gene trap cell lines in mouse. Nucleic Acids Res. 2006 Jan 01; 34(Database issue):D642-8. PMID: 16381950.
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    101. Turnbull L, Zhou HZ, Swigart PM, Turcato S, Karliner JS, Conklin BR, Simpson PC, Baker AJ. Sustained preconditioning induced by cardiac transgenesis with the tetracycline transactivator. Am J Physiol Heart Circ Physiol. 2006 Mar; 290(3):H1103-9. PMID: 16243914.
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    102. Zambon AC, Zhang L, Minovitsky S, Kanter JR, Prabhakar S, Salomonis N, Vranizan K, Dubchak I, Conklin BR, Insel PA. Gene expression patterns define key transcriptional events in cell-cycle regulation by cAMP and protein kinase A. Proc Natl Acad Sci U S A. 2005 Jun 14; 102(24):8561-6. PMID: 15939874.
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    103. McCloskey DT, Turnbull L, Swigart PM, Zambon AC, Turcato S, Joho S, Grossman W, Conklin BR, Simpson PC, Baker AJ. Cardiac transgenesis with the tetracycline transactivator changes myocardial function and gene expression. Physiol Genomics. 2005 Jun 16; 22(1):118-26. PMID: 15797971.
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    104. Scearce-Levie K, Lieberman MD, Elliott HH, Conklin BR. Engineered G protein coupled receptors reveal independent regulation of internalization, desensitization and acute signaling. BMC Biol. 2005 Feb 11; 3:3. PMID: 15707483.
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    105. Salomonis N, Cotte N, Zambon AC, Pollard KS, Vranizan K, Doniger SW, Dolganov G, Conklin BR. Identifying genetic networks underlying myometrial transition to labor. Genome Biol. 2005; 6(2):R12. PMID: 15693941.
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    106. Srinivasan S, Lubrano-Berthelier C, Govaerts C, Picard F, Santiago P, Conklin BR, Vaisse C. Constitutive activity of the melanocortin-4 receptor is maintained by its N-terminal domain and plays a role in energy homeostasis in humans. J Clin Invest. 2004 Oct; 114(8):1158-64. PMID: 15489963.
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    107. Skarnes WC, von Melchner H, Wurst W, Hicks G, Nord AS, Cox T, Young SG, Ruiz P, Soriano P, Tessier-Lavigne M, Conklin BR, Stanford WL, Rossant J. A public gene trap resource for mouse functional genomics. Nat Genet. 2004 Jun; 36(6):543-4. PMID: 15167922.
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    108. Zambon AC, McDearmon EL, Salomonis N, Vranizan KM, Johansen KL, Adey D, Takahashi JS, Schambelan M, Conklin BR. Time- and exercise-dependent gene regulation in human skeletal muscle. Genome Biol. 2003; 4(10):R61. PMID: 14519196.
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    109. Srinivasan S, Vaisse C, Conklin BR. Engineering the melanocortin-4 receptor to control G(s) signaling in vivo. Ann N Y Acad Sci. 2003 Jun; 994:225-32. PMID: 12851320.
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    110. Doniger SW, Salomonis N, Dahlquist KD, Vranizan K, Lawlor SC, Conklin BR. MAPPFinder: using Gene Ontology and GenMAPP to create a global gene-expression profile from microarray data. Genome Biol. 2003; 4(1):R7. PMID: 12540299.
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    111. Segal MR, Dahlquist KD, Conklin BR. Regression approaches for microarray data analysis. J Comput Biol. 2003; 10(6):961-80. PMID: 14980020.
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    112. Dahlquist KD, Salomonis N, Vranizan K, Lawlor SC, Conklin BR. GenMAPP, a new tool for viewing and analyzing microarray data on biological pathways. Nat Genet. 2002 May; 31(1):19-20. PMID: 11984561.
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    113. Scearce-Levie K, Coward P, Redfern CH, Conklin BR. Tools for dissecting signaling pathways in vivo: receptors activated solely by synthetic ligands. Methods Enzymol. 2002; 343:232-48. PMID: 11665570.
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    114. Scearce-Levie K, Coward P, Redfern CH, Conklin BR. Engineering receptors activated solely by synthetic ligands (RASSLs). Trends Pharmacol Sci. 2001 Aug; 22(8):414-20. PMID: 11479004.
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    115. Baker AJ, Redfern CH, Harwood MD, Simpson PC, Conklin BR. Abnormal contraction caused by expression of G(i)-coupled receptor in transgenic model of dilated cardiomyopathy. Am J Physiol Heart Circ Physiol. 2001 Apr; 280(4):H1653-9. PMID: 11247776.
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    116. Chang MA, Horner JW, Conklin BR, DePinho RA, Bok D, Zack DJ. Tetracycline-inducible system for photoreceptor-specific gene expression. Invest Ophthalmol Vis Sci. 2000 Dec; 41(13):4281-7. PMID: 11095627.
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    117. Redfern CH, Degtyarev MY, Kwa AT, Salomonis N, Cotte N, Nanevicz T, Fidelman N, Desai K, Vranizan K, Lee EK, Coward P, Shah N, Warrington JA, Fishman GI, Bernstein D, Baker AJ, Conklin BR. Conditional expression of a Gi-coupled receptor causes ventricular conduction delay and a lethal cardiomyopathy. Proc Natl Acad Sci U S A. 2000 Apr 25; 97(9):4826-31. PMID: 10781088.
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    118. Charles H. Redfern, Michael Y. Degtyarev, Kavin Desai, Andrew Kwa, Glenn I. Fishman, Daniel Bernstein, Anthony J. Baker, Bruce R. Conklin. Genomic analysis of an inducible and reversible model of dilated cardiomyopathy. Journal of Cardiac Failure. 1999 Sep 1; 5(3):14.
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    119. Nathalie Cotte, Charles H. Redfern, Peter Mikus, Nathan Salomonis, Michael Y. Degtyarev, Peter S. Coward, Andrew Kwa, Tetsuo Shioi, Nila Shah, Tim McKinsey, Eric Olson, Seigo Izumo, Janet Warrington, Anthony J. Baker, Bruce R. Conklin. A critical period of vulnerability to hyperactive Gq signaling revealed by inducible and heart-specific expression of Gq* in transgenic mice. Journal of Cardiac Failure. 1999 Sep 1; 5(3):12.
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    120. Coward P, Chan SD, Wada HG, Humphries GM, Conklin BR. Chimeric G proteins allow a high-throughput signaling assay of Gi-coupled receptors. Anal Biochem. 1999 Jun 01; 270(2):242-8. PMID: 10334841.
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    121. Redfern CH, Coward P, Degtyarev MY, Lee EK, Kwa AT, Hennighausen L, Bujard H, Fishman GI, Conklin BR. Conditional expression and signaling of a specifically designed Gi-coupled receptor in transgenic mice. Nat Biotechnol. 1999 Feb; 17(2):165-9. PMID: 10052353.
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    122. Coward P, Wada HG, Falk MS, Chan SD, Meng F, Akil H, Conklin BR. Controlling signaling with a specifically designed Gi-coupled receptor. Proc Natl Acad Sci U S A. 1998 Jan 06; 95(1):352-7. PMID: 9419379.
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    123. Kostenis E, Degtyarev MY, Conklin BR, Wess J. The N-terminal extension of Galphaq is critical for constraining the selectivity of receptor coupling. J Biol Chem. 1997 Aug 01; 272(31):19107-10. PMID: 9235898.
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    124. Kostenis E, Conklin BR, Wess J. Molecular basis of receptor/G protein coupling selectivity studied by coexpression of wild type and mutant m2 muscarinic receptors with mutant G alpha(q) subunits. Biochemistry. 1997 Feb 11; 36(6):1487-95. PMID: 9063897.
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    125. Burstein ES, Bräuner-Osborne H, Spalding TA, Conklin BR, Brann MR. Interactions of muscarinic receptors with the heterotrimeric G proteins Gq and G12: transduction of proliferative signals. J Neurochem. 1997 Feb; 68(2):525-33. PMID: 9003037.
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    126. Conklin BR, Herzmark P, Ishida S, Voyno-Yasenetskaya TA, Sun Y, Farfel Z, Bourne HR. Carboxyl-terminal mutations of Gq alpha and Gs alpha that alter the fidelity of receptor activation. Mol Pharmacol. 1996 Oct; 50(4):885-90. PMID: 8863834.
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    127. Liu J, Blin N, Conklin BR, Wess J. Molecular mechanisms involved in muscarinic acetylcholine receptor-mediated G protein activation studied by insertion mutagenesis. J Biol Chem. 1996 Mar 15; 271(11):6172-8. PMID: 8626406.
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    128. Liu J, Conklin BR, Blin N, Yun J, Wess J. Identification of a receptor/G-protein contact site critical for signaling specificity and G-protein activation. Proc Natl Acad Sci U S A. 1995 Dec 05; 92(25):11642-6. PMID: 8524820.
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    129. Chabre O, Conklin BR, Brandon S, Bourne HR, Limbird LE. Coupling of the alpha 2A-adrenergic receptor to multiple G-proteins. A simple approach for estimating receptor-G-protein coupling efficiency in a transient expression system. J Biol Chem. 1994 Feb 25; 269(8):5730-4. PMID: 7907086.
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    130. Voyno-Yasenetskaya T, Conklin BR, Gilbert RL, Hooley R, Bourne HR, Barber DL. G alpha 13 stimulates Na-H exchange. J Biol Chem. 1994 Feb 18; 269(7):4721-4. PMID: 8106440.
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    131. Conklin BR, Bourne HR. Homeostatic signals. Marriage of the flytrap and the serpent. Nature. 1994 Jan 06; 367(6458):22. PMID: 8107768.
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    132. Conklin BR, Bourne HR. Mouse coat colour reconsidered. Nature. 1993 Jul 08; 364(6433):110. PMID: 8321283.
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    133. Lustig KD, Conklin BR, Herzmark P, Taussig R, Bourne HR. Type II adenylylcyclase integrates coincident signals from Gs, Gi, and Gq. J Biol Chem. 1993 Jul 05; 268(19):13900-5. PMID: 8390980.
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    134. Conklin BR, Bourne HR. Structural elements of G alpha subunits that interact with G beta gamma, receptors, and effectors. Cell. 1993 May 21; 73(4):631-41. PMID: 8388779.
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    135. Conklin BR, Farfel Z, Lustig KD, Julius D, Bourne HR. Substitution of three amino acids switches receptor specificity of Gq alpha to that of Gi alpha. Nature. 1993 May 20; 363(6426):274-6. PMID: 8387644.
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    136. Chabre O, Conklin BR, Lin HY, Lodish HF, Wilson E, Ives HE, Catanzariti L, Hemmings BA, Bourne HR. A recombinant calcitonin receptor independently stimulates 3',5'-cyclic adenosine monophosphate and Ca2+/inositol phosphate signaling pathways. Mol Endocrinol. 1992 Apr; 6(4):551-6. PMID: 1316547.
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    137. Federman AD, Conklin BR, Schrader KA, Reed RR, Bourne HR. Hormonal stimulation of adenylyl cyclase through Gi-protein beta gamma subunits. Nature. 1992 Mar 12; 356(6365):159-61. PMID: 1312225.
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    138. Wong YH, Conklin BR, Bourne HR. Gz-mediated hormonal inhibition of cyclic AMP accumulation. Science. 1992 Jan 17; 255(5042):339-42. PMID: 1347957.
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    139. Conklin BR, Chabre O, Wong YH, Federman AD, Bourne HR. Recombinant Gq alpha. Mutational activation and coupling to receptors and phospholipase C. J Biol Chem. 1992 Jan 05; 267(1):31-4. PMID: 1309740.
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    140. Bourne HR, Lustig KD, Wong YH, Conklin BR. Detection of coincident signals by G proteins and adenylyl cyclase. Cold Spring Harb Symp Quant Biol. 1992; 57:145-8. PMID: 1339653.
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    141. Felder CC, Ma AL, Conklin BR. Carbachol-induced reverse transformation of Chinese hamster ovary cells transfected with and expressing the m5 muscarinic acetylcholine receptor. FEBS Lett. 1989 Mar 13; 245(1-2):75-9. PMID: 2466702.
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    142. Conklin BR, Brann MR, Buckley NJ, Ma AL, Bonner TI, Axelrod J. Stimulation of arachidonic acid release and inhibition of mitogenesis by cloned genes for muscarinic receptor subtypes stably expressed in A9 L cells. Proc Natl Acad Sci U S A. 1988 Nov; 85(22):8698-702. PMID: 2847172.
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    143. Conklin BR, Burch RM, Steranka LR, Axelrod J. Distinct bradykinin receptors mediate stimulation of prostaglandin synthesis by endothelial cells and fibroblasts. J Pharmacol Exp Ther. 1988 Feb; 244(2):646-9. PMID: 2894457.
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