Daniel Minor, JR, PhD

Title(s)Professor, Cardiovascular Research Inst
SchoolSchool of Medicine
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    Collapse Biography 
    Collapse Education and Training
    University of Pennsylvania, Philadelphia, PAB.A. magna cum laude05/1989Biochemistry (Honors) Biophysics (Honors)
    Massachusetts Institute of Technology, Cambridge, MAPh.D.02/1996Biochemistry
    MRC Laboratory of Molecular Biology, Cambridge, UKpostdoc09/1996Ion channel structure
    University of California San Francisco, San Francisco, CApostdoc12/2000Ion channel structure and function

    Collapse Research 
    Collapse Research Activities and Funding
    Expanding the Chemical Biology of K2P Channels with Selective Cellular and In vivo Probes
    NIH/NIMH R01MH116278Mar 1, 2018 - Jan 31, 2021
    Role: Co-Principal Investigator
    Genetic and chemical biological studies of K2P structure, function, and modulation
    NIH/NIMH R01MH093603Mar 1, 2011 - Feb 28, 2021
    Role: Principal Investigator
    Center for Structure of Membrane Proteins
    NIH/NIGMS U54GM094625Sep 30, 2010 - Jun 30, 2015
    Role: Co-Investigator
    Structural studies of CaV alpha2delta subunits and interaction with anti-nocicept
    NIH/NINDS R21NS065448Sep 1, 2009 - Aug 31, 2013
    Role: Principal Investigator
    Structural Studies of Ion Channel Assembly and Signaling Complexes
    NIH/NIDCD R01DC007664Jul 1, 2005 - Jan 31, 2022
    Role: Principal Investigator
    Structure and function of voltage-gated calcium channels
    NIH/NHLBI R01HL080050May 1, 2005 - Mar 31, 2018
    Role: Principal Investigator
    Membrane Protein Expression Center
    NIH/NIGMS P50GM073210Sep 24, 2004 - Aug 31, 2014
    Role: Co-Investigator
    Discovery and characterization of ion channel modulators
    NIH/NINDS R01NS049272Jul 1, 2004 - May 31, 2010
    Role: Principal Investigator
    Eukaryotic Ion Channel Expression for Structural Study
    NIH/NINDS R21NS047299Jan 1, 2004 - Dec 31, 2006
    Role: Principal Investigator
    Bio-Organic Biomedical Mass Spectrometry Resource
    NIH P41RR001614Mar 1, 1982 - May 31, 2015
    Role: Co-Investigator

    Collapse ORNG Applications 
    Collapse Websites
    Collapse In The News

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. Yen TJ, Lolicato M, Thomas-Tran R, Du Bois J, Minor DL. Structure of the saxiphilin:saxitoxin (STX) complex reveals a convergent molecular recognition strategy for paralytic toxins. Sci Adv. 2019 Jun; 5(6):eaax2650. PMID: 31223657.
      View in: PubMed
    2. Kimberlin CR, Meshcheriakova A, Palty R, Raveh A, Karbat I, Reuveny E, Minor DL. SARAF Luminal Domain Structure Reveals a Novel Domain-Swapped ß-Sandwich Fold Important for SOCE Modulation. J Mol Biol. 2019 Jul 12; 431(15):2869-2883. PMID: 31082439.
      View in: PubMed
    3. Abderemane-Ali F, Findeisen F, Rossen ND, Minor DL. A Selectivity Filter Gate Controls Voltage-Gated Calcium Channel Calcium-Dependent Inactivation. Neuron. 2019 Mar 20; 101(6):1134-1149.e3. PMID: 30733149.
      View in: PubMed
    4. Chang A, Abderemane-Ali F, Hura GL, Rossen ND, Gate RE, Minor DL. A Calmodulin C-Lobe Ca2+-Dependent Switch Governs Kv7 Channel Function. Neuron. 2018 Feb 21; 97(4):836-852.e6. PMID: 29429937.
      View in: PubMed
    5. Arrigoni C, Minor DL. Global versus local mechanisms of temperature sensing in ion channels. Pflugers Arch. 2018 05; 470(5):733-744. PMID: 29340775.
      View in: PubMed
    6. Ely LK, Lolicato M, David T, Lowe K, Kim YC, Samuel D, Bessette P, Garcia JL, Mikita T, Minor DL, Coughlin SR. Structural Basis for Activity and Specificity of an Anticoagulant Anti-FXIa Monoclonal Antibody and a Reversal Agent. Structure. 2018 02 06; 26(2):187-198.e4. PMID: 29336885.
      View in: PubMed
    7. Dang S, Feng S, Tien J, Peters CJ, Bulkley D, Lolicato M, Zhao J, Zuberbühler K, Ye W, Qi L, Chen T, Craik CS, Jan YN, Minor DL, Cheng Y, Jan LY. Cryo-EM structures of the TMEM16A calcium-activated chloride channel. Nature. 2017 12 21; 552(7685):426-429. PMID: 29236684.
      View in: PubMed
    8. Lolicato M, Arrigoni C, Mori T, Sekioka Y, Bryant C, Clark KA, Minor DL. K2P2.1 (TREK-1)-activator complexes reveal a cryptic selectivity filter binding site. Nature. 2017 07 20; 547(7663):364-368. PMID: 28693035.
      View in: PubMed
    9. Findeisen F, Campiglio M, Jo H, Abderemane-Ali F, Rumpf CH, Pope L, Rossen ND, Flucher BE, DeGrado WF, Minor DL. Stapled Voltage-Gated Calcium Channel (CaV) a-Interaction Domain (AID) Peptides Act As Selective Protein-Protein Interaction Inhibitors of CaV Function. ACS Chem Neurosci. 2017 06 21; 8(6):1313-1326. PMID: 28278376.
      View in: PubMed
    10. Arrigoni C, Rohaim A, Shaya D, Findeisen F, Stein RA, Nurva SR, Mishra S, Mchaourab HS, Minor DL. Unfolding of a Temperature-Sensitive Domain Controls Voltage-Gated Channel Activation. Cell. 2016 Feb 25; 164(5):922-36. PMID: 26919429; PMCID: PMC4769381 [Available on 02/25/17].
    11. Minor DL. Let It Go and Open Up, an Ensemble of Ion Channel Active States. Cell. 2016 Feb 11; 164(4):597-8. PMID: 26871624.
      View in: PubMed
    12. Lolicato M, Riegelhaupt PM, Arrigoni C, Clark KA, Minor DL. Transmembrane helix straightening and buckling underlies activation of mechanosensitive and thermosensitive K(2P) channels. Neuron. 2014 Dec 17; 84(6):1198-212. PMID: 25500157; PMCID: PMC4270892 [Available on 12/17/15].
    13. Payandeh J, Minor DL. Bacterial voltage-gated sodium channels (BacNa(V)s) from the soil, sea, and salt lakes enlighten molecular mechanisms of electrical signaling and pharmacology in the brain and heart. J Mol Biol. 2015 Jan 16; 427(1):3-30. PMID: 25158094; PMCID: PMC4277928.
    14. Bagriantsev SN, Chatelain FC, Clark KA, Alagem N, Reuveny E, Minor DL. Tethered protein display identifies a novel Kir3.2 (GIRK2) regulator from protein scaffold libraries. ACS Chem Neurosci. 2014 Sep 17; 5(9):812-22. PMID: 25028803; PMCID: PMC4176385.
    15. Isacoff EY, Jan LY, Minor DL. Conduits of life's spark: a perspective on ion channel research since the birth of neuron. Neuron. 2013 Oct 30; 80(3):658-74. PMID: 24183018; PMCID: PMC3867263.
    16. Shaya D, Findeisen F, Abderemane-Ali F, Arrigoni C, Wong S, Nurva SR, Loussouarn G, Minor DL. Structure of a prokaryotic sodium channel pore reveals essential gating elements and an outer ion binding site common to eukaryotic channels. J Mol Biol. 2014 Jan 23; 426(2):467-83. PMID: 24120938; PMCID: PMC3947372.
    17. Findeisen F, Rumpf CH, Minor DL. Apo states of calmodulin and CaBP1 control CaV1 voltage-gated calcium channel function through direct competition for the IQ domain. J Mol Biol. 2013 Sep 09; 425(17):3217-34. PMID: 23811053; PMCID: PMC3839849.
    18. Bagriantsev SN, Ang KH, Gallardo-Godoy A, Clark KA, Arkin MR, Renslo AR, Minor DL. A high-throughput functional screen identifies small molecule regulators of temperature- and mechano-sensitive K2P channels. ACS Chem Biol. 2013 Aug 16; 8(8):1841-51. PMID: 23738709; PMCID: PMC3747594.
    19. Tien J, Lee HY, Minor DL, Jan YN, Jan LY. Identification of a dimerization domain in the TMEM16A calcium-activated chloride channel (CaCC). Proc Natl Acad Sci U S A. 2013 Apr 16; 110(16):6352-7. PMID: 23576756; PMCID: PMC3631655.
    20. Bagriantsev SN, Minor DL. Using yeast to study potassium channel function and interactions with small molecules. Methods Mol Biol. 2013; 995:31-42. PMID: 23494370; PMCID: PMC3950311.
    21. Xu Q, Chang A, Tolia A, Minor DL. Structure of a Ca(2+)/CaM:Kv7.4 (KCNQ4) B-helix complex provides insight into M current modulation. J Mol Biol. 2013 Jan 23; 425(2):378-94. PMID: 23178170; PMCID: PMC3540129.
    22. Bagriantsev SN, Clark KA, Minor DL. Metabolic and thermal stimuli control K(2P)2.1 (TREK-1) through modular sensory and gating domains. EMBO J. 2012 Aug 01; 31(15):3297-308. PMID: 22728824; PMCID: PMC3411076.
    23. Liu L, Kohout SC, Xu Q, Müller S, Kimberlin CR, Isacoff EY, Minor DL. A glutamate switch controls voltage-sensitive phosphatase function. Nat Struct Mol Biol. 2012 May 06; 19(6):633-41. PMID: 22562138.
      View in: PubMed
    24. Bagriantsev SN, Peyronnet R, Clark KA, Honoré E, Minor DL. Multiple modalities converge on a common gate to control K2P channel function. EMBO J. 2011 Jul 15; 30(17):3594-606. PMID: 21765396; PMCID: PMC3181481.
    25. Shaya D, Kreir M, Robbins RA, Wong S, Hammon J, Brüggemann A, Minor DL. Voltage-gated sodium channel (NaV) protein dissection creates a set of functional pore-only proteins. Proc Natl Acad Sci U S A. 2011 Jul 26; 108(30):12313-8. PMID: 21746903; PMCID: PMC3145705.
    26. Findeisen F, Tolia A, Arant R, Kim EY, Isacoff E, Minor DL. Calmodulin overexpression does not alter Cav1.2 function or oligomerization state. Channels (Austin). 2011 Jul-Aug; 5(4):320-4. PMID: 21712653; PMCID: PMC3225732.
    27. Findeisen F, Minor DL. Structural basis for the differential effects of CaBP1 and calmodulin on Ca(V)1.2 calcium-dependent inactivation. Structure. 2010 Dec 08; 18(12):1617-31. PMID: 21134641.
      View in: PubMed
    28. Minor DL, Findeisen F. Progress in the structural understanding of voltage-gated calcium channel (CaV) function and modulation. Channels (Austin). 2010 Nov-Dec; 4(6):459-74. PMID: 21139419; PMCID: PMC3018750.
    29. Kim EY, Rumpf CH, Van Petegem F, Arant RJ, Findeisen F, Cooley ES, Isacoff EY, Minor DL. Multiple C-terminal tail Ca(2+)/CaMs regulate Ca(V)1.2 function but do not mediate channel dimerization. EMBO J. 2010 Dec 01; 29(23):3924-38. PMID: 20953164.
      View in: PubMed
    30. Bagriantsev SN, Minor DL. Small molecule ion channel match making: a natural fit for new ASIC ligands. Neuron. 2010 Oct 06; 68(1):1-3. PMID: 20920784; PMCID: PMC2981581.
    31. Kohout SC, Bell SC, Liu L, Xu Q, Minor DL, Isacoff EY. Electrochemical coupling in the voltage-dependent phosphatase Ci-VSP. Nat Chem Biol. 2010 May; 6(5):369-75. PMID: 20364128; PMCID: PMC2857593.
    32. Chatelain FC, Gazzarrini S, Fujiwara Y, Arrigoni C, Domigan C, Ferrara G, Pantoja C, Thiel G, Moroni A, Minor DL. Selection of inhibitor-resistant viral potassium channels identifies a selectivity filter site that affects barium and amantadine block. PLoS One. 2009 Oct 16; 4(10):e7496. PMID: 19834614.
      View in: PubMed
    33. Xu Q, Minor DL. Crystal structure of a trimeric form of the K(V)7.1 (KCNQ1) A-domain tail coiled-coil reveals structural plasticity and context dependent changes in a putative coiled-coil trimerization motif. Protein Sci. 2009 Oct; 18(10):2100-14. PMID: 19693805.
      View in: PubMed
    34. Minor DL. Searching for interesting channels: pairing selection and molecular evolution methods to study ion channel structure and function. Mol Biosyst. 2009 Aug; 5(8):802-10. PMID: 19603113; PMCID: PMC3016960.
    35. Findeisen F, Minor DL. Disruption of the IS6-AID linker affects voltage-gated calcium channel inactivation and facilitation. J Gen Physiol. 2009 Mar; 133(3):327-43. PMID: 19237593; PMCID: PMC2654080.
    36. Stroud RM, Choe S, Holton J, Kaback HR, Kwiatkowski W, Minor DL, Riek R, Sali A, Stahlberg H, Harries W. 2007 annual progress report synopsis of the Center for Structures of Membrane Proteins. J Struct Funct Genomics. 2009 Apr; 10(2):193-208. PMID: 19148774; PMCID: PMC2705707.
    37. Hammon J, Palanivelu DV, Chen J, Patel C, Minor DL. A green fluorescent protein screen for identification of well-expressed membrane proteins from a cohort of extremophilic organisms. Protein Sci. 2009 Jan; 18(1):121-33. PMID: 19177357; PMCID: PMC2708023.
    38. Kim EY, Rumpf CH, Fujiwara Y, Cooley ES, Van Petegem F, Minor DL. Structures of CaV2 Ca2+/CaM-IQ domain complexes reveal binding modes that underlie calcium-dependent inactivation and facilitation. Structure. 2008 Oct 08; 16(10):1455-67. PMID: 18940602.
      View in: PubMed
    39. Fujiwara Y, Minor DL. X-ray crystal structure of a TRPM assembly domain reveals an antiparallel four-stranded coiled-coil. J Mol Biol. 2008 Nov 21; 383(4):854-70. PMID: 18782578.
      View in: PubMed
    40. Van Petegem F, Duderstadt KE, Clark KA, Wang M, Minor DL. Alanine-scanning mutagenesis defines a conserved energetic hotspot in the CaValpha1 AID-CaVbeta interaction site that is critical for channel modulation. Structure. 2008 Feb; 16(2):280-94. PMID: 18275819; PMCID: PMC3018278.
    41. Minor DL. Puzzle plugged by protein pore plasticity. Mol Cell. 2007 May 25; 26(4):459-60. PMID: 17531803.
      View in: PubMed
    42. Minor DL. The neurobiologist's guide to structural biology: a primer on why macromolecular structure matters and how to evaluate structural data. Neuron. 2007 May 24; 54(4):511-33. PMID: 17521566; PMCID: PMC3011226.
    43. Howard RJ, Clark KA, Holton JM, Minor DL. Structural insight into KCNQ (Kv7) channel assembly and channelopathy. Neuron. 2007 Mar 01; 53(5):663-75. PMID: 17329207.
      View in: PubMed
    44. Pioletti M, Findeisen F, Hura GL, Minor DL. Three-dimensional structure of the KChIP1-Kv4.3 T1 complex reveals a cross-shaped octamer. Nat Struct Mol Biol. 2006 Nov; 13(11):987-95. PMID: 17057713.
      View in: PubMed
    45. Tsuruda PR, Julius D, Minor DL. Coiled coils direct assembly of a cold-activated TRP channel. Neuron. 2006 Jul 20; 51(2):201-12. PMID: 16846855.
      View in: PubMed
    46. Minor DL. A sensitive channel family replete with sense and motion. Nat Struct Mol Biol. 2006 May; 13(5):388-90. PMID: 16738606.
      View in: PubMed
    47. Van Petegem F, Chatelain FC, Minor DL. Insights into voltage-gated calcium channel regulation from the structure of the CaV1.2 IQ domain-Ca2+/calmodulin complex. Nat Struct Mol Biol. 2005 Dec; 12(12):1108-15. PMID: 16299511.
      View in: PubMed
    48. Chatelain FC, Alagem N, Xu Q, Pancaroglu R, Reuveny E, Minor DL. The pore helix dipole has a minor role in inward rectifier channel function. Neuron. 2005 Sep 15; 47(6):833-43. PMID: 16157278.
      View in: PubMed
    49. Van Petegem F, Clark KA, Chatelain FC, Minor DL. Structure of a complex between a voltage-gated calcium channel beta-subunit and an alpha-subunit domain. Nature. 2004 Jun 10; 429(6992):671-5. PMID: 15141227.
      View in: PubMed
    50. Walden H, Podgorski MS, Huang DT, Miller DW, Howard RJ, Minor DL, Holton JM, Schulman BA. The structure of the APPBP1-UBA3-NEDD8-ATP complex reveals the basis for selective ubiquitin-like protein activation by an E1. Mol Cell. 2003 Dec; 12(6):1427-37. PMID: 14690597.
      View in: PubMed