Loading...

Frances Brodsky, PhD

TitleProfessor
InstitutionUniversity of California San Francisco
DepartmentBioengineering
AddressLocation Required
Varies CA 00000
Phone415-476-6406
vCardDownload vCard

    Collapse Biography 
    Collapse Awards and Honors
    1975Phi Beta Kappa, Junior year Camille and Henry Dreyfus Fellowship for Summer Research
    1976Marshall Scholarship
    1980Damon Runyon-Walter Winchell Fund Postdoctoral Fellowship
    1987Pharmaceutical Manufacturers Association Fund, Research Starter Grant
    1988Pew Scholars Program
    McGill University2005Boehringer-Ingleheim Lecturer
    University of Queensland2005Honorary Professor
    University of California, San Francisco2005Commencement Speaker for Graduate Programs
    National Institutes of Health2006Margaret Pittman Lecturer
    King's College, Cambridge2006Visiting Fellowship
    American Society of Cell Biology2007Senior Career Award, Women in Cell Biology
    University of Massachusetts2008Kai Lin Memorial Lecturer
    Lysosomes Gordon Research Conference2008Elected Vice-Chair
    Lysosomes Gordon Research Conference2010Elected Chair
    Harris-Manchester College, Oxford University2010Lord Harris Visiting Professor
    Karolinska Institute Stockholm, Sweden2011Diabetes Symposium Invited Speaker

    Collapse Overview 
    Collapse Overview
    Clathrin is a self-assembling protein that controls the formation of intracellular transport vesicles required for several key membrane traffic pathways. These include receptor-mediated endocytosis and biogenesis of lysosomes and secretory granules. Thus clathrin plays a critical role in cell growth control, antigen presentation, neuronal function and hormone secretion. Malfunction of clathrin pathways is associated with development of cancer, heart disease and neuronal defects. Clathrin-mediated pathways are also subverted by certain viruses and intracellular bacteria to facilitate their infection. A novel isoform of clathrin (CHC22) that is primarily expressed in skeletal muscle, cardiac muscle and fat has been recently characterized by the Brodsky laboratory. Its tissue-specific function is to package the GLUT4 glucose transporter into intracellular vesicles that are released in response to insulin stimulation, leading to clearance of glucose from the bloodstream. This CHC22 clathrin isoform is therefore associated with pathways that are defective in type 2 diabetes. The focus of the Brodsky laboratory since 1982 has been to define the biochemistry, regulation, evolution and physiological function of the clathrin subunits and their isoforms, focusing on their role in disease states, as well as in normal cellular physiology. The laboratory has made significant contributions in these areas over the years and maintains an international reputation for expertise on clathrin structure and function.
    There are four current projects in the laboratory. These projects aim to define the comparative functions of the two clathrin heavy chain (CHC) isoforms (CHC17 and CHC22) and the two clathrin light chain isoforms (LCa and LCb). The regulation of CHC17 clathrin function by signaling pathways and the cooperation of CHC17 clathrin with the actin cytoskeleton through clathrin light chain interaction with the actin-binding Hip proteins is being investigated in the context of cell adhesion and migration. Establishing the mechanistic role of CHC22 clathrin in GLUT4 transport and pathways that malfunction in type 2 diabetes is also a major goal for the laboratory.


    Collapse Research 
    Collapse Research Activities and Funding
    Biochemistry and Cell Biology of CHC22 Clathrin
    NIH/NIDDK R01DK095663Dec 1, 2012 - Nov 30, 2016
    Role: Principal Investigator
    CHC22 CLATHRIN FUNCTION IN HUMAN GLUCOSE METABOLISM
    NIH/NIDDK R56DK083589Sep 20, 2009 - Aug 31, 2011
    Role: Principal Investigator
    2008-2010 Lysosomes &Endocytosis Gordon Research Conference
    NIH/NIGMS R13GM084519May 1, 2008 - Apr 30, 2011
    Role: Principal Investigator
    Human Natural Killer Cell Biology
    NIH/NIAID P01AI064520Sep 16, 2005 - Feb 28, 2011
    Role: Principal Investigator
    STRUCTURAL BASIS FOR CLATHRIN FUNCTION
    NIH/NIGMS R01GM061954Jul 1, 2000 - Jun 30, 2004
    Role: Principal Investigator
    HUMAN IMMUNE CELL MATURATION DURING ONTOGENY AND INFECTI
    NIH/NIAID P01AI045865Sep 30, 1999 - Aug 31, 2003
    Role: Principal Investigator
    MICROPARTICULATE ACCESSIBILITY TO THE ENDOCYTIC PATHWAY
    NIH/NIGMS R01GM057657Aug 1, 1997 - Jul 31, 2003
    Role: Principal Investigator
    MOLECULAR BASIS OF DRUG TRANSPORT IN THE INTESTINE
    NIH/NIGMS R01GM057656Aug 1, 1997 - Jul 31, 2001
    Role: Co-Investigator
    STRUCTURAL BASIS FOR CLATHRIN FUNCTION
    NIH/NIGMS R21GM055143Jul 1, 1997 - Jun 30, 2000
    Role: Principal Investigator
    ANTIGEN PRESENTATION BY THE MHC--MOLECULAR MECHANISM
    NIH/NIGMS R01GM046205May 1, 1992 - Apr 30, 1997
    Role: Principal Investigator
    Cellular Regulation of Clathrin Assembly and Function
    NIH/NIGMS R01GM038093Feb 1, 1988 - Aug 31, 2015
    Role: Principal Investigator
    PHARMACOKINETICS/PHARMACODYNAMICS
    NIH/NIGMS P50GM026691Aug 1, 1979 - Jul 31, 1998
    Role: Co-Investigator
    MULTIPURPOSE ARTHRITIS AND MUSCULOSKELETAL DISEASES CTR
    NIH/NIAMS P60AR020684Sep 15, 1977 - Dec 31, 2003
    Role: Co-Investigator

    Collapse ORNG Applications 
    Collapse Websites
    Collapse In The News

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
    List All   |   Timeline
    1. Dannhauser PN, Camus SM, Sakamoto K, Sadacca LA, Torres JA, Camus MD, Briant K, Vassilopoulos S, Rothnie A, Smith CJ, Brodsky FM. CHC22 and CHC17 clathrins have distinct biochemical properties and display differential regulation and function. J Biol Chem. 2017 Dec 22; 292(51):20834-20844. PMID: 29097553.
      View in: PubMed
    2. Wu S, Majeed SR, Evans TM, Camus MD, Wong NM, Schollmeier Y, Park M, Muppidi JR, Reboldi A, Parham P, Cyster JG, Brodsky FM. Clathrin light chains' role in selective endocytosis influences antibody isotype switching. Proc Natl Acad Sci U S A. 2016 08 30; 113(35):9816-21. PMID: 27540116; PMCID: PMC5024586 [Available on 02/28/17].
      View in: PubMed, PubMed Central
    3. Goyos A, Guethlein LA, Horowitz A, Hilton HG, Gleimer M, Brodsky FM, Parham P. A Distinctive Cytoplasmic Tail Contributes to Low Surface Expression and Intracellular Retention of the Patr-AL MHC Class I Molecule. J Immunol. 2015 Oct 15; 195(8):3725-36. PMID: 26371256; PMCID: PMC4592827 [Available on 10/15/16].
      View in: PubMed, PubMed Central
    4. Brodsky FM, Sosa RT, Ybe JA, O'Halloran TJ. Unconventional functions for clathrin, ESCRTs, and other endocytic regulators in the cytoskeleton, cell cycle, nucleus, and beyond: links to human disease. Cold Spring Harb Perspect Biol. 2014 Sep 02; 6(9):a017004. PMID: 25183831; PMCID: PMC4142963.
      View in: PubMed, PubMed Central
    5. Majeed SR, Vasudevan L, Chen CY, Luo Y, Torres JA, Evans TM, Sharkey A, Foraker AB, Wong NM, Esk C, Freeman TA, Moffett A, Keen JH, Brodsky FM. Clathrin light chains are required for the gyrating-clathrin recycling pathway and thereby promote cell migration. Nat Commun. 2014 May 23; 5:3891. PMID: 24852344; PMCID: PMC4050264.
      View in: PubMed, PubMed Central
    6. Vassilopoulos S, Gentil C, Lainé J, Buclez PO, Franck A, Ferry A, Précigout G, Roth R, Heuser JE, Brodsky FM, Garcia L, Bonne G, Voit T, Piétri-Rouxel F, Bitoun M. Actin scaffolding by clathrin heavy chain is required for skeletal muscle sarcomere organization. J Cell Biol. 2014 May 12; 205(3):377-93. PMID: 24798732; PMCID: PMC4018784.
      View in: PubMed, PubMed Central
    7. Sullivan CS, Scheib JL, Ma Z, Dang RP, Schafer JM, Hickman FE, Brodsky FM, Ravichandran KS, Carter BD. The adaptor protein GULP promotes Jedi-1-mediated phagocytosis through a clathrin-dependent mechanism. Mol Biol Cell. 2014 Jun 15; 25(12):1925-36. PMID: 24743597; PMCID: PMC4055271.
      View in: PubMed, PubMed Central
    8. Asensio CS, Sirkis DW, Maas JW, Egami K, To TL, Brodsky FM, Shu X, Cheng Y, Edwards RH. Self-assembly of VPS41 promotes sorting required for biogenesis of the regulated secretory pathway. Dev Cell. 2013 Nov 25; 27(4):425-37. PMID: 24210660; PMCID: PMC3974617.
      View in: PubMed, PubMed Central
    9. Hoshino S, Sakamoto K, Vassilopoulos S, Camus SM, Griffin CA, Esk C, Torres JA, Ohkoshi N, Ishii A, Tamaoka A, Funke BH, Kucherlapati R, Margeta M, Rando TA, Brodsky FM. The CHC22 clathrin-GLUT4 transport pathway contributes to skeletal muscle regeneration. PLoS One. 2013; 8(10):e77787. PMID: 24204966; PMCID: PMC3813726.
      View in: PubMed, PubMed Central
    10. Brodsky F. Frances Brodsky: so you think you know all about clathrin? Interview by Caitlin Sedwick. J Cell Biol. 2013 Sep 16; 202(6):830-1. PMID: 24043697; PMCID: PMC3776341.
      View in: PubMed, PubMed Central
    11. Stachowiak JC, Brodsky FM, Miller EA. A cost-benefit analysis of the physical mechanisms of membrane curvature. Nat Cell Biol. 2013 Sep; 15(9):1019-27. PMID: 23999615; PMCID: PMC3813008.
      View in: PubMed, PubMed Central
    12. Holmes BB, DeVos SL, Kfoury N, Li M, Jacks R, Yanamandra K, Ouidja MO, Brodsky FM, Marasa J, Bagchi DP, Kotzbauer PT, Miller TM, Papy-Garcia D, Diamond MI. Heparan sulfate proteoglycans mediate internalization and propagation of specific proteopathic seeds. Proc Natl Acad Sci U S A. 2013 Aug 13; 110(33):E3138-47. PMID: 23898162; PMCID: PMC3746848.
      View in: PubMed, PubMed Central
    13. Young A, Stoilova-McPhie S, Rothnie A, Vallis Y, Harvey-Smith P, Ranson N, Kent H, Brodsky FM, Pearse BM, Roseman A, Smith CJ. Hsc70-induced changes in clathrin-auxilin cage structure suggest a role for clathrin light chains in cage disassembly. Traffic. 2013 Sep; 14(9):987-96. PMID: 23710728; PMCID: PMC3776051.
      View in: PubMed, PubMed Central
    14. Hom N, Mehta KR, Chou T, Foraker AB, Brodsky FM, Kirshenbaum K, Montclare JK. Anisotropic nanocrystal arrays organized on protein lattices formed by recombinant clathrin fragments. J Mater Chem. 2012 Dec 28; 22(44):23335-23339. PMID: 23836951.
      View in: PubMed
    15. Bonazzi M, Kühbacher A, Toledo-Arana A, Mallet A, Vasudevan L, Pizarro-Cerdá J, Brodsky FM, Cossart P. A common clathrin-mediated machinery co-ordinates cell-cell adhesion and bacterial internalization. Traffic. 2012 Dec; 13(12):1653-66. PMID: 22984946; PMCID: PMC3760411.
      View in: PubMed, PubMed Central
    16. Foraker AB, Camus SM, Evans TM, Majeed SR, Chen CY, Taner SB, Corrêa IR, Doxsey SJ, Brodsky FM. Clathrin promotes centrosome integrity in early mitosis through stabilization of centrosomal ch-TOG. J Cell Biol. 2012 Aug 20; 198(4):591-605. PMID: 22891263; PMCID: PMC3514040.
      View in: PubMed, PubMed Central
    17. Brodsky FM. Diversity of clathrin function: new tricks for an old protein. Annu Rev Cell Dev Biol. 2012; 28:309-36. PMID: 22831640.
      View in: PubMed
    18. Brodsky FM, Thattai M, Mayor S. Evolutionary cell biology: Lessons from diversity. Nat Cell Biol. 2012 Jun 29; 14(7):651. PMID: 22743706.
      View in: PubMed
    19. Bonazzi M, Vasudevan L, Mallet A, Sachse M, Sartori A, Prevost MC, Roberts A, Taner SB, Wilbur JD, Brodsky FM, Cossart P. Clathrin phosphorylation is required for actin recruitment at sites of bacterial adhesion and internalization. J Cell Biol. 2011 Oct 31; 195(3):525-36. PMID: 22042622; PMCID: PMC3206339.
      View in: PubMed, PubMed Central
    20. Stoddart A, Tennant TR, Fernald AA, Anastasi J, Brodsky FM, Le Beau MM. The clathrin-binding domain of CALM-AF10 alters the phenotype of myeloid neoplasms in mice. Oncogene. 2012 Jan 26; 31(4):494-506. PMID: 21706055; PMCID: PMC3204175.
      View in: PubMed, PubMed Central
    21. Shieh JC, Schaar BT, Srinivasan K, Brodsky FM, McConnell SK. Endocytosis regulates cell soma translocation and the distribution of adhesion proteins in migrating neurons. PLoS One. 2011 Mar 22; 6(3):e17802. PMID: 21445347; PMCID: PMC3062553.
      View in: PubMed, PubMed Central
    22. Taner SB, Pando MJ, Roberts A, Schellekens J, Marsh SG, Malmberg KJ, Parham P, Brodsky FM. Interactions of NK cell receptor KIR3DL1*004 with chaperones and conformation-specific antibody reveal a functional folded state as well as predominant intracellular retention. J Immunol. 2011 Jan 01; 186(1):62-72. PMID: 21115737; PMCID: PMC3129036.
      View in: PubMed, PubMed Central
    23. Wilbur JD, Hwang PK, Ybe JA, Lane M, Sellers BD, Jacobson MP, Fletterick RJ, Brodsky FM. Conformation switching of clathrin light chain regulates clathrin lattice assembly. Dev Cell. 2010 May 18; 18(5):841-8. PMID: 20493816; PMCID: PMC2975025.
      View in: PubMed, PubMed Central
    24. Wilbur JD, Hwang PK, Brodsky FM, Fletterick RJ. Accommodation of structural rearrangements in the huntingtin-interacting protein 1 coiled-coil domain. Acta Crystallogr D Biol Crystallogr. 2010 Mar; 66(Pt 3):314-8. PMID: 20179344; PMCID: PMC2827350.
      View in: PubMed, PubMed Central
    25. Esk C, Chen CY, Johannes L, Brodsky FM. The clathrin heavy chain isoform CHC22 functions in a novel endosomal sorting step. J Cell Biol. 2010 Jan 11; 188(1):131-44. PMID: 20065094; PMCID: PMC2812854.
      View in: PubMed, PubMed Central
    26. Vassilopoulos S, Esk C, Hoshino S, Brodsky FM. [The CHC22 human clathrin heavy chain isoform, intracellular traffic of the glucose transporter GLUT4, and type 2 diabetes]. Med Sci (Paris). 2010 Jan; 26(1):33-6. PMID: 20132771.
      View in: PubMed
    27. Sharma D, Bastard K, Guethlein LA, Norman PJ, Yawata N, Yawata M, Pando M, Thananchai H, Dong T, Rowland-Jones S, Brodsky FM, Parham P. Dimorphic motifs in D0 and D1+D2 domains of killer cell Ig-like receptor 3DL1 combine to form receptors with high, moderate, and no avidity for the complex of a peptide derived from HIV and HLA-A*2402. J Immunol. 2009 Oct 01; 183(7):4569-82. PMID: 19752231; PMCID: PMC2827337.
      View in: PubMed, PubMed Central
    28. Vassilopoulos S, Esk C, Hoshino S, Funke BH, Chen CY, Plocik AM, Wright WE, Kucherlapati R, Brodsky FM. A role for the CHC22 clathrin heavy-chain isoform in human glucose metabolism. Science. 2009 May 29; 324(5931):1192-6. PMID: 19478182; PMCID: PMC2975026.
      View in: PubMed, PubMed Central
    29. Wilbur JD, Chen CY, Manalo V, Hwang PK, Fletterick RJ, Brodsky FM. Actin binding by Hip1 (huntingtin-interacting protein 1) and Hip1R (Hip1-related protein) is regulated by clathrin light chain. J Biol Chem. 2008 Nov 21; 283(47):32870-9. PMID: 18790740; PMCID: PMC2583295.
      View in: PubMed, PubMed Central
    30. Kirkham M, Nixon SJ, Howes MT, Abi-Rached L, Wakeham DE, Hanzal-Bayer M, Ferguson C, Hill MM, Fernandez-Rojo M, Brown DA, Hancock JF, Brodsky FM, Parton RG. Evolutionary analysis and molecular dissection of caveola biogenesis. J Cell Sci. 2008 Jun 15; 121(Pt 12):2075-86. PMID: 18505796.
      View in: PubMed
    31. Sowinski S, Jolly C, Berninghausen O, Purbhoo MA, Chauveau A, Köhler K, Oddos S, Eissmann P, Brodsky FM, Hopkins C, Onfelt B, Sattentau Q, Davis DM. Membrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission. Nat Cell Biol. 2008 Feb; 10(2):211-9. PMID: 18193035.
      View in: PubMed
    32. Knuehl C, Chen CY, Manalo V, Hwang PK, Ota N, Brodsky FM. Novel binding sites on clathrin and adaptors regulate distinct aspects of coat assembly. Traffic. 2006 Dec; 7(12):1688-700. PMID: 17052248.
      View in: PubMed
    33. Chen TT, Li L, Chung DH, Allen CD, Torti SV, Torti FM, Cyster JG, Chen CY, Brodsky FM, Niemi EC, Nakamura MC, Seaman WE, Daws MR. TIM-2 is expressed on B cells and in liver and kidney and is a receptor for H-ferritin endocytosis. J Exp Med. 2005 Oct 03; 202(7):955-65. PMID: 16203866; PMCID: PMC2213179.
      View in: PubMed, PubMed Central
    34. Wakeham DE, Abi-Rached L, Towler MC, Wilbur JD, Parham P, Brodsky FM. Clathrin heavy and light chain isoforms originated by independent mechanisms of gene duplication during chordate evolution. Proc Natl Acad Sci U S A. 2005 May 17; 102(20):7209-14. PMID: 15883369; PMCID: PMC1091751.
      View in: PubMed, PubMed Central
    35. Wilbur JD, Hwang PK, Brodsky FM. New faces of the familiar clathrin lattice. Traffic. 2005 Apr; 6(4):346-50. PMID: 15752139.
      View in: PubMed
    36. Stoddart A, Jackson AP, Brodsky FM. Plasticity of B cell receptor internalization upon conditional depletion of clathrin. Mol Biol Cell. 2005 May; 16(5):2339-48. PMID: 15716350; PMCID: PMC1087239.
      View in: PubMed, PubMed Central
    37. Brodsky FM. Cell biology: clathrin's Achilles' ankle. Nature. 2004 Dec 02; 432(7017):568-9. PMID: 15577897.
      View in: PubMed
    38. Chen CY, Brodsky FM. Huntingtin-interacting protein 1 (Hip1) and Hip1-related protein (Hip1R) bind the conserved sequence of clathrin light chains and thereby influence clathrin assembly in vitro and actin distribution in vivo. J Biol Chem. 2005 Feb 18; 280(7):6109-17. PMID: 15533940.
      View in: PubMed
    39. Niiro H, Allam A, Stoddart A, Brodsky FM, Marshall AJ, Clark EA. The B lymphocyte adaptor molecule of 32 kilodaltons (Bam32) regulates B cell antigen receptor internalization. J Immunol. 2004 Nov 01; 173(9):5601-9. PMID: 15494510.
      View in: PubMed
    40. Towler MC, Gleeson PA, Hoshino S, Rahkila P, Manalo V, Ohkoshi N, Ordahl C, Parton RG, Brodsky FM. Clathrin isoform CHC22, a component of neuromuscular and myotendinous junctions, binds sorting nexin 5 and has increased expression during myogenesis and muscle regeneration. Mol Biol Cell. 2004 Jul; 15(7):3181-95. PMID: 15133132; PMCID: PMC452575.
      View in: PubMed, PubMed Central
    41. Crotzer VL, Mabardy AS, Weiss A, Brodsky FM. T cell receptor engagement leads to phosphorylation of clathrin heavy chain during receptor internalization. J Exp Med. 2004 Apr 05; 199(7):981-91. PMID: 15067034.
      View in: PubMed
    42. Towler MC, Kaufman SJ, Brodsky FM. Membrane traffic in skeletal muscle. Traffic. 2004 Mar; 5(3):129-39. PMID: 15086789.
      View in: PubMed
    43. Wakeham DE, Chen CY, Greene B, Hwang PK, Brodsky FM. Clathrin self-assembly involves coordinated weak interactions favorable for cellular regulation. EMBO J. 2003 Oct 01; 22(19):4980-90. PMID: 14517237; PMCID: PMC204494.
      View in: PubMed, PubMed Central
    44. Knuehl C, Brodsky FM. The long and short of adaptor appendages. Nat Struct Biol. 2003 Aug; 10(8):580-2. PMID: 12886287.
      View in: PubMed
    45. Chen CY, Reese ML, Hwang PK, Ota N, Agard D, Brodsky FM. Clathrin light and heavy chain interface: alpha-helix binding superhelix loops via critical tryptophans. EMBO J. 2002 Nov 15; 21(22):6072-82. PMID: 12426379; PMCID: PMC137186.
      View in: PubMed, PubMed Central
    46. Stoddart A, Dykstra ML, Brown BK, Song W, Pierce SK, Brodsky FM. Lipid rafts unite signaling cascades with clathrin to regulate BCR internalization. Immunity. 2002 Oct; 17(4):451-62. PMID: 12387739.
      View in: PubMed
    47. Yang D, Buchholz F, Huang Z, Goga A, Chen CY, Brodsky FM, Bishop JM. Short RNA duplexes produced by hydrolysis with Escherichia coli RNase III mediate effective RNA interference in mammalian cells. Proc Natl Acad Sci U S A. 2002 Jul 23; 99(15):9942-7. PMID: 12096193; PMCID: PMC126604.
      View in: PubMed, PubMed Central
    48. Bennett EM, Chen CY, Engqvist-Goldstein AE, Drubin DG, Brodsky FM. Clathrin hub expression dissociates the actin-binding protein Hip1R from coated pits and disrupts their alignment with the actin cytoskeleton. Traffic. 2001 Nov; 2(11):851-8. PMID: 11733052.
      View in: PubMed
    49. Bennett EM, Lin SX, Towler MC, Maxfield FR, Brodsky FM. Clathrin hub expression affects early endosome distribution with minimal impact on receptor sorting and recycling. Mol Biol Cell. 2001 Sep; 12(9):2790-9. PMID: 11553717; PMCID: PMC59713.
      View in: PubMed, PubMed Central
    50. Molinete M, Dupuis S, Brodsky FM, Halban PA. Role of clathrin in the regulated secretory pathway of pancreatic beta-cells. J Cell Sci. 2001 Aug; 114(Pt 16):3059-66. PMID: 11686308.
      View in: PubMed
    51. Liu SH, Towler MC, Chen E, Chen CY, Song W, Apodaca G, Brodsky FM. A novel clathrin homolog that co-distributes with cytoskeletal components functions in the trans-Golgi network. EMBO J. 2001 Jan 15; 20(1-2):272-84. PMID: 11226177; PMCID: PMC140205.
      View in: PubMed, PubMed Central
    52. Brodsky FM, Chen CY, Knuehl C, Towler MC, Wakeham DE. Biological basket weaving: formation and function of clathrin-coated vesicles. Annu Rev Cell Dev Biol. 2001; 17:517-68. PMID: 11687498.
      View in: PubMed
    53. Beattie EC, Howe CL, Wilde A, Brodsky FM, Mobley WC. NGF signals through TrkA to increase clathrin at the plasma membrane and enhance clathrin-mediated membrane trafficking. J Neurosci. 2000 Oct 01; 20(19):7325-33. PMID: 11007890.
      View in: PubMed
    54. Ybe JA, Wakeham DE, Brodsky FM, Hwang PK. Molecular structures of proteins involved in vesicle fusion. Traffic. 2000 Jun; 1(6):474-9. PMID: 11208133.
      View in: PubMed
    55. Wakeham DE, Ybe JA, Brodsky FM, Hwang PK. Molecular structures of proteins involved in vesicle coat formation. Traffic. 2000 May; 1(5):393-8. PMID: 11208125.
      View in: PubMed
    56. Jahn R, Brodsky FM. Transport of proteins and membrane in eukaryotic cells (Göttingen, September 29-October 1, 1999). Traffic. 2000 Feb; 1(2):187-8. PMID: 11208099.
      View in: PubMed
    57. Greene B, Liu SH, Wilde A, Brodsky FM. Complete reconstitution of clathrin basket formation with recombinant protein fragments: adaptor control of clathrin self-assembly. Traffic. 2000 Jan; 1(1):69-75. PMID: 11208061.
      View in: PubMed
    58. Altschuler Y, Liu S, Katz L, Tang K, Hardy S, Brodsky F, Apodaca G, Mostov K. ADP-ribosylation factor 6 and endocytosis at the apical surface of Madin-Darby canine kidney cells. J Cell Biol. 1999 Oct 04; 147(1):7-12. PMID: 10508850; PMCID: PMC2164974.
      View in: PubMed, PubMed Central
    59. Ybe JA, Brodsky FM, Hofmann K, Lin K, Liu SH, Chen L, Earnest TN, Fletterick RJ, Hwang PK. Clathrin self-assembly is mediated by a tandemly repeated superhelix. Nature. 1999 May 27; 399(6734):371-5. PMID: 10360576.
      View in: PubMed
    60. Bennett EM, Bennink JR, Yewdell JW, Brodsky FM. Cutting edge: adenovirus E19 has two mechanisms for affecting class I MHC expression. J Immunol. 1999 May 01; 162(9):5049-52. PMID: 10227971.
      View in: PubMed
    61. Brodsky FM. Stealth, sabotage and exploitation. Immunol Rev. 1999 Apr; 168:5-11. PMID: 10399060.
      View in: PubMed
    62. Brodsky FM, Lem L, Solache A, Bennett EM. Human pathogen subversion of antigen presentation. Immunol Rev. 1999 Apr; 168:199-215. PMID: 10399076.
      View in: PubMed
    63. Wilde A, Beattie EC, Lem L, Riethof DA, Liu SH, Mobley WC, Soriano P, Brodsky FM. EGF receptor signaling stimulates SRC kinase phosphorylation of clathrin, influencing clathrin redistribution and EGF uptake. Cell. 1999 Mar 05; 96(5):677-87. PMID: 10089883.
      View in: PubMed
    64. Lem L, Riethof DA, Scidmore-Carlson M, Griffiths GM, Hackstadt T, Brodsky FM. Enhanced interaction of HLA-DM with HLA-DR in enlarged vacuoles of hereditary and infectious lysosomal diseases. J Immunol. 1999 Jan 01; 162(1):523-32. PMID: 9886429.
      View in: PubMed
    65. Ybe JA, Greene B, Liu SH, Pley U, Parham P, Brodsky FM. Clathrin self-assembly is regulated by three light-chain residues controlling the formation of critical salt bridges. EMBO J. 1998 Aug 10; 17(5):1297-303. PMID: 9482727; PMCID: PMC1170478.
      View in: PubMed, PubMed Central
    66. Lu X, Yu H, Liu SH, Brodsky FM, Peterlin BM. Interactions between HIV1 Nef and vacuolar ATPase facilitate the internalization of CD4. Immunity. 1998 May; 8(5):647-56. PMID: 9620685.
      View in: PubMed
    67. Liu SH, Marks MS, Brodsky FM. A dominant-negative clathrin mutant differentially affects trafficking of molecules with distinct sorting motifs in the class II major histocompatibility complex (MHC) pathway. J Cell Biol. 1998 Mar 09; 140(5):1023-37. PMID: 9490717; PMCID: PMC2132696.
      View in: PubMed, PubMed Central
    68. Bresnahan PA, Barber LD, Brodsky FM. Localization of class I histocompatibility molecule assembly by subfractionation of the early secretory pathway. Hum Immunol. 1997 Apr 01; 53(2):129-39. PMID: 9129970.
      View in: PubMed
    69. Mallet WG, Brodsky FM. A membrane-associated protein complex with selective binding to the clathrin coat adaptor AP1. J Cell Sci. 1996 Dec; 109 ( Pt 13):3059-68. PMID: 9004040.
      View in: PubMed
    70. Wilde A, Brodsky FM. In vivo phosphorylation of adaptors regulates their interaction with clathrin. J Cell Biol. 1996 Nov; 135(3):635-45. PMID: 8909539; PMCID: PMC2121074.
      View in: PubMed, PubMed Central
    71. Brodsky FM, Lem L, Bresnahan PA. Antigen processing and presentation. Tissue Antigens. 1996 Jun; 47(6):464-71. PMID: 8813734.
      View in: PubMed
    72. Liu SH, Wong ML, Craik CS, Brodsky FM. Regulation of clathrin assembly and trimerization defined using recombinant triskelion hubs. Cell. 1995 Oct 20; 83(2):257-67. PMID: 7585943.
      View in: PubMed
    73. Pley UM, Hill BL, Alibert C, Brodsky FM, Parham P. The interaction of calmodulin with clathrin-coated vesicles, triskelions, and light chains. Localization of a binding site. J Biol Chem. 1995 Feb 03; 270(5):2395-402. PMID: 7836475.
      View in: PubMed
    74. Acton SL, Wong DH, Parham P, Brodsky FM, Jackson AP. Alteration of clathrin light chain expression by transfection and gene disruption. Mol Biol Cell. 1993 Jun; 4(6):647-60. PMID: 8374173; PMCID: PMC300971.
      View in: PubMed, PubMed Central
    75. Chang MP, Mallet WG, Mostov KE, Brodsky FM. Adaptor self-aggregation, adaptor-receptor recognition and binding of alpha-adaptin subunits to the plasma membrane contribute to recruitment of adaptor (AP2) components of clathrin-coated pits. EMBO J. 1993 May; 12(5):2169-80. PMID: 8491205; PMCID: PMC413438.
      View in: PubMed, PubMed Central
    76. Beck KA, Chang M, Brodsky FM, Keen JH. Clathrin assembly protein AP-2 induces aggregation of membrane vesicles: a possible role for AP-2 in endosome formation. J Cell Biol. 1992 Nov; 119(4):787-96. PMID: 1358896; PMCID: PMC2289704.
      View in: PubMed, PubMed Central
    77. Wong DH, Brodsky FM. 100-kD proteins of Golgi- and trans-Golgi network-associated coated vesicles have related but distinct membrane binding properties. J Cell Biol. 1992 Jun; 117(6):1171-9. PMID: 1607381; PMCID: PMC2289504.
      View in: PubMed, PubMed Central
    78. Brodsky FM. Antigen processing and presentation: close encounters in the endocytic pathway. Trends Cell Biol. 1992 Apr; 2(4):109-15. PMID: 14732015.
      View in: PubMed
    79. Näthke IS, Heuser J, Lupas A, Stock J, Turck CW, Brodsky FM. Folding and trimerization of clathrin subunits at the triskelion hub. Cell. 1992 Mar 06; 68(5):899-910. PMID: 1547490.
      View in: PubMed
    80. Brodsky FM, Hill BL, Acton SL, Näthke I, Wong DH, Ponnambalam S, Parham P. Clathrin light chains: arrays of protein motifs that regulate coated-vesicle dynamics. Trends Biochem Sci. 1991 Jun; 16(6):208-13. PMID: 1909824.
      View in: PubMed
    81. VanBuskirk AM, DeNagel DC, Guagliardi LE, Brodsky FM, Pierce SK. Cellular and subcellular distribution of PBP72/74, a peptide-binding protein that plays a role in antigen processing. J Immunol. 1991 Jan 15; 146(2):500-6. PMID: 1987275.
      View in: PubMed
    82. Brodsky FM, Guagliardi LE. The cell biology of antigen processing and presentation. Annu Rev Immunol. 1991; 9:707-44. PMID: 1910692.
      View in: PubMed
    83. Näthke I, Hill BL, Parham P, Brodsky FM. The calcium-binding site of clathrin light chains. J Biol Chem. 1990 Oct 25; 265(30):18621-7. PMID: 2211724.
      View in: PubMed
    84. Acton SL, Brodsky FM. Predominance of clathrin light chain LCb correlates with the presence of a regulated secretory pathway. J Cell Biol. 1990 Oct; 111(4):1419-26. PMID: 2211818; PMCID: PMC2116225.
      View in: PubMed, PubMed Central
    85. Mahaffey DT, Peeler JS, Brodsky FM, Anderson RG. Clathrin-coated pits contain an integral membrane protein that binds the AP-2 subunit with high affinity. J Biol Chem. 1990 Sep 25; 265(27):16514-20. PMID: 1975814.
      View in: PubMed
    86. Wong DH, Ignatius MJ, Parosky G, Parham P, Trojanowski JQ, Brodsky FM. Neuron-specific expression of high-molecular-weight clathrin light chain. J Neurosci. 1990 Sep; 10(9):3025-31. PMID: 2204686.
      View in: PubMed
    87. Draper RK, Goda Y, Brodsky FM, Pfeffer SR. Antibodies to clathrin inhibit endocytosis but not recycling to the trans Golgi network in vitro. Science. 1990 Jun 22; 248(4962):1539-41. PMID: 2163108.
      View in: PubMed
    88. Guagliardi LE, Koppelman B, Blum JS, Marks MS, Cresswell P, Brodsky FM. Co-localization of molecules involved in antigen processing and presentation in an early endocytic compartment. Nature. 1990 Jan 11; 343(6254):133-9. PMID: 2404209.
      View in: PubMed
    89. Chin DJ, Straubinger RM, Acton S, Näthke I, Brodsky FM. 100-kDa polypeptides in peripheral clathrin-coated vesicles are required for receptor-mediated endocytosis. Proc Natl Acad Sci U S A. 1989 Dec; 86(23):9289-93. PMID: 2574457; PMCID: PMC298480.
      View in: PubMed, PubMed Central
    90. Szöllösi J, Damjanovich S, Balàzs M, Nagy P, Trón L, Fulwyler MJ, Brodsky FM. Physical association between MHC class I and class II molecules detected on the cell surface by flow cytometric energy transfer. J Immunol. 1989 Jul 01; 143(1):208-13. PMID: 2732468.
      View in: PubMed
    91. Mahaffey DT, Moore MS, Brodsky FM, Anderson RG. Coat proteins isolated from clathrin coated vesicles can assemble into coated pits. J Cell Biol. 1989 May; 108(5):1615-24. PMID: 2565904; PMCID: PMC2115561.
      View in: PubMed, PubMed Central
    92. Parham P, Brodsky FM, Drickamer K. The occurrence of disulphide bonds in purified clathrin light chains. Biochem J. 1989 Feb 01; 257(3):775-81. PMID: 2930486; PMCID: PMC1135655.
      View in: PubMed, PubMed Central
    93. Brodsky FM, Koppelman B, Blum JS, Marks MS, Cresswell P, Guagliardi L. Intracellular colocalization of molecules involved in antigen processing and presentation by B cells. Cold Spring Harb Symp Quant Biol. 1989; 54 Pt 1:319-31. PMID: 2700939.
      View in: PubMed
    94. Brodsky FM. Living with clathrin: its role in intracellular membrane traffic. Science. 1988 Dec 09; 242(4884):1396-402. PMID: 2904698.
      View in: PubMed
    95. Hill BL, Drickamer K, Brodsky FM, Parham P. Identification of the phosphorylation sites of clathrin light chain LCb. J Biol Chem. 1988 Apr 25; 263(12):5499-501. PMID: 3128543.
      View in: PubMed
    96. Brodsky FM. Monoclonal antibodies as magic bullets. Pharm Res. 1988 Jan; 5(1):1-9. PMID: 3072552.
      View in: PubMed
    97. Blank GS, Brodsky FM. Clathrin assembly involves a light chain-binding region. J Cell Biol. 1987 Nov; 105(5):2011-9. PMID: 2445759; PMCID: PMC2114853.
      View in: PubMed, PubMed Central
    98. Doxsey SJ, Brodsky FM, Blank GS, Helenius A. Inhibition of endocytosis by anti-clathrin antibodies. Cell. 1987 Jul 31; 50(3):453-63. PMID: 3111717.
      View in: PubMed
    99. Clayberger C, Krensky AM, McIntyre BW, Koller TD, Parham P, Brodsky F, Linn DJ, Evans EL. Identification and characterization of two novel lymphocyte function-associated antigens, L24 and L25. J Immunol. 1987 Mar 01; 138(5):1510-4. PMID: 3492556.
      View in: PubMed
    100. Brodsky FM, Galloway CJ, Blank GS, Jackson AP, Seow HF, Drickamer K, Parham P. Localization of clathrin light-chain sequences mediating heavy-chain binding and coated vesicle diversity. Nature. 1987 Mar 12-18; 326(6109):203-5. PMID: 2434865.
      View in: PubMed
    101. Blank GS, Brodsky FM. Site-specific disruption of clathrin assembly produces novel structures. EMBO J. 1986 Sep; 5(9):2087-95. PMID: 3096714; PMCID: PMC1167086.
      View in: PubMed, PubMed Central
    102. Brodsky FM. Clathrin structure characterized with monoclonal antibodies. I. Analysis of multiple antigenic sites. J Cell Biol. 1985 Dec; 101(6):2047-54. PMID: 2415533; PMCID: PMC2114023.
      View in: PubMed, PubMed Central
    103. Brodsky FM. Clathrin structure characterized with monoclonal antibodies. II. Identification of in vivo forms of clathrin. J Cell Biol. 1985 Dec; 101(6):2055-62. PMID: 4066749; PMCID: PMC2114020.
      View in: PubMed, PubMed Central
    104. Holmes N, Biermann JS, Brodsky FM, Bharucha D, Parham P. Comparison of the primary structures of clathrin light chains from bovine brain and adrenal gland by peptide mapping. EMBO J. 1984 Jul; 3(7):1621-7. PMID: 6745244; PMCID: PMC557568.
      View in: PubMed, PubMed Central
    105. Brodsky FM, Parham P. Polymorphism in clathrin light chains from different tissues. J Mol Biol. 1983 Jun 15; 167(1):197-204. PMID: 6864801.
      View in: PubMed
    106. Kirchhausen T, Harrison SC, Parham P, Brodsky FM. Location and distribution of the light chains in clathrin trimers. Proc Natl Acad Sci U S A. 1983 May; 80(9):2481-5. PMID: 6189124; PMCID: PMC393849.
      View in: PubMed, PubMed Central
    107. Brodsky FM, Holmes NJ, Parham P. Tropomyosin-like properties of clathrin light chains allow a rapid, high-yield purification. J Cell Biol. 1983 Mar; 96(3):911-4. PMID: 6833387; PMCID: PMC2112400.
      View in: PubMed, PubMed Central
    108. Parham P, Androlewicz MJ, Brodsky FM, Holmes NJ, Ways JP. Monoclonal antibodies: purification, fragmentation and application to structural and functional studies of class I MHC antigens. J Immunol Methods. 1982 Sep 17; 53(2):133-73. PMID: 6183363.
      View in: PubMed
    109. Brodsky FM, Parham P. Monomorphic anti-HLA-A,B,C monoclonal antibodies detecting molecular subunits and combinatorial determinants. J Immunol. 1982 Jan; 128(1):129-35. PMID: 6172474.
      View in: PubMed
    110. Brodsky FM, Parham P. Evolution of HLA antigenic determinants: species cross-reactions of monoclonal antibodies. Immunogenetics. 1982; 15(2):151-66. PMID: 6174442.
      View in: PubMed
    111. Parham P, Brodsky FM. Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28. Hum Immunol. 1981 Dec; 3(4):277-99. PMID: 7035415.
      View in: PubMed
    112. Brodsky FM, Stone WH, Parham P. Of cows and men: a comparative study of histocompatibility antigens. Hum Immunol. 1981 Oct; 3(2):143-52. PMID: 7035414.
      View in: PubMed
    113. McMichael AJ, Parham P, Rust N, Brodsky F. A monoclonal antibody that recognizes an antigenic determinant shared by HLA A2 and B17. Hum Immunol. 1980 Sep; 1(2):121-9. PMID: 6167544.
      View in: PubMed
    114. McMichael AJ, Parham P, Brodsky FM, Pilch JR. Influenza virus-specific cytotoxic T lymphocytes recognize HLA-molecules. Blocking by monoclonal anti-HLA antibodies. J Exp Med. 1980 Aug 01; 152(2 Pt 2):195s-203s. PMID: 6967937.
      View in: PubMed
    115. Brodsky FM, Parham P, Bodmer WF. Monoclonal antibodies to HLA--DRw determinants. Tissue Antigens. 1980 Jul; 16(1):30-48. PMID: 6162228.
      View in: PubMed
    116. Brodsky FM, Bodmer WF, Parham P. Characterization of a monoclonal anti-beta 2-microglobulin antibody and its use in the genetic and biochemical analysis of major histocompatibility antigens. Eur J Immunol. 1979 Jul; 9(7):536-45. PMID: 91522.
      View in: PubMed
    117. Parham P, Sehgal PK, Brodsky FM. Anti-HLA-A,B,C monoclonal antibodies with no alloantigenic specificity in humans define polymorphisms in other primate species. Nature. 1979 Jun 14; 279(5714):639-41. PMID: 88017.
      View in: PubMed
    118. Brodsky FM, Parham P, Barnstable CJ, Crumpton MJ, Bodmer WF. Monoclonal antibodies for analysis of the HLA system. Immunol Rev. 1979; 47:3-61. PMID: 95015.
      View in: PubMed
    Frances's Networks
    Concepts
    Derived automatically from this person's publications.
    See all (517) concept(s)
    _
    Co-Authors
    People in Profiles who have published with this person.
    See all (19) people
    _
    Related Authors
    People who share related concepts with this person.
    See all (60) people
    _
    ↑