Loading...

Frances Brodsky, PhD

TitleProfessor
SchoolUCSF School of Pharmacy
DepartmentBioengineering
Address513 Parnassus Ave
San Francisco CA 94143
Phone415-476-6406
vCardDownload vCard

    Collapse Biography 
    Collapse Awards and Honors
    1975Phi Beta Kappa, Junior year Camille and Henry Dreyfus Fellowship for Summer Research
    1976Marshall Scholarship
    1980Damon Runyon-Walter Winchell Fund Postdoctoral Fellowship
    1987Pharmaceutical Manufacturers Association Fund, Research Starter Grant
    1988Pew Scholars Program
    McGill University2005Boehringer-Ingleheim Lecturer
    University of Queensland2005Honorary Professor
    University of California, San Francisco2005Commencement Speaker for Graduate Programs
    National Institutes of Health2006Margaret Pittman Lecturer
    King's College, Cambridge2006Visiting Fellowship
    American Society of Cell Biology2007Senior Career Award, Women in Cell Biology
    University of Massachusetts2008Kai Lin Memorial Lecturer
    Lysosomes Gordon Research Conference2008Elected Vice-Chair
    Lysosomes Gordon Research Conference2010Elected Chair
    Harris-Manchester College, Oxford University2010Lord Harris Visiting Professor
    Karolinska Institute Stockholm, Sweden2011Diabetes Symposium Invited Speaker

    Collapse Overview 
    Collapse Overview
    Clathrin is a self-assembling protein that controls the formation of intracellular transport vesicles required for several key membrane traffic pathways. These include receptor-mediated endocytosis and biogenesis of lysosomes and secretory granules. Thus clathrin plays a critical role in cell growth control, antigen presentation, neuronal function and hormone secretion. Malfunction of clathrin pathways is associated with development of cancer, heart disease and neuronal defects. Clathrin-mediated pathways are also subverted by certain viruses and intracellular bacteria to facilitate their infection. A novel isoform of clathrin (CHC22) that is primarily expressed in skeletal muscle, cardiac muscle and fat has been recently characterized by the Brodsky laboratory. Its tissue-specific function is to package the GLUT4 glucose transporter into intracellular vesicles that are released in response to insulin stimulation, leading to clearance of glucose from the bloodstream. This CHC22 clathrin isoform is therefore associated with pathways that are defective in type 2 diabetes. The focus of the Brodsky laboratory since 1982 has been to define the biochemistry, regulation, evolution and physiological function of the clathrin subunits and their isoforms, focusing on their role in disease states, as well as in normal cellular physiology. The laboratory has made significant contributions in these areas over the years and maintains an international reputation for expertise on clathrin structure and function.
    There are four current projects in the laboratory. These projects aim to define the comparative functions of the two clathrin heavy chain (CHC) isoforms (CHC17 and CHC22) and the two clathrin light chain isoforms (LCa and LCb). The regulation of CHC17 clathrin function by signaling pathways and the cooperation of CHC17 clathrin with the actin cytoskeleton through clathrin light chain interaction with the actin-binding Hip proteins is being investigated in the context of cell adhesion and migration. Establishing the mechanistic role of CHC22 clathrin in GLUT4 transport and pathways that malfunction in type 2 diabetes is also a major goal for the laboratory.


    Collapse Research 
    Collapse Research Activities and Funding
    Biochemistry and Cell Biology of CHC22 Clathrin
    NIH/NIDDK R01DK095663Dec 1, 2012 - Nov 30, 2016
    Role: Principal Investigator
    CHC22 CLATHRIN FUNCTION IN HUMAN GLUCOSE METABOLISM
    NIH/NIDDK R56DK083589Sep 20, 2009 - Aug 31, 2011
    Role: Principal Investigator
    2008-2010 Lysosomes &Endocytosis Gordon Research Conference
    NIH/NIGMS R13GM084519May 1, 2008 - Apr 30, 2011
    Role: Principal Investigator
    Human Natural Killer Cell Biology
    NIH/NIAID P01AI064520Sep 16, 2005 - Feb 28, 2011
    Role: Principal Investigator
    STRUCTURAL BASIS FOR CLATHRIN FUNCTION
    NIH/NIGMS R01GM061954Jul 1, 2000 - Jun 30, 2004
    Role: Principal Investigator
    HUMAN IMMUNE CELL MATURATION DURING ONTOGENY AND INFECTI
    NIH/NIAID P01AI045865Sep 30, 1999 - Aug 31, 2003
    Role: Principal Investigator
    MICROPARTICULATE ACCESSIBILITY TO THE ENDOCYTIC PATHWAY
    NIH/NIGMS R01GM057657Aug 1, 1997 - Jul 31, 2003
    Role: Principal Investigator
    MOLECULAR BASIS OF DRUG TRANSPORT IN THE INTESTINE
    NIH/NIGMS R01GM057656Aug 1, 1997 - Jul 31, 2001
    Role: Co-Investigator
    STRUCTURAL BASIS FOR CLATHRIN FUNCTION
    NIH/NIGMS R21GM055143Jul 1, 1997 - Jun 30, 2000
    Role: Principal Investigator
    ANTIGEN PRESENTATION BY THE MHC--MOLECULAR MECHANISM
    NIH/NIGMS R01GM046205May 1, 1992 - Apr 30, 1997
    Role: Principal Investigator
    Cellular Regulation of Clathrin Assembly and Function
    NIH/NIGMS R01GM038093Feb 1, 1988 - Aug 31, 2015
    Role: Principal Investigator
    PHARMACOKINETICS/PHARMACODYNAMICS
    NIH/NIGMS P50GM026691Aug 1, 1979 - Jul 31, 1998
    Role: Co-Investigator
    MULTIPURPOSE ARTHRITIS AND MUSCULOSKELETAL DISEASES CTR
    NIH/NIAMS P60AR020684Sep 15, 1977 - Dec 31, 2003
    Role: Co-Investigator

    Collapse ORNG Applications 
    Collapse Websites
    Collapse In The News
    Collapse Global Health

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
    List All   |   Timeline
    1. Wu S, Majeed SR, Evans TM, Camus MD, Wong NM, Schollmeier Y, Park M, Muppidi JR, Reboldi A, Parham P, Cyster JG, Brodsky F, et al. Clathrin light chains' role in selective endocytosis influences antibody isotype switching. Proc Natl Acad Sci U S A. 2016 Aug 18. PMID: 27540116.
      View in: PubMed
    2. Goyos A, Guethlein LA, Horowitz A, Hilton HG, Gleimer M, Brodsky F, Parham P. A Distinctive Cytoplasmic Tail Contributes to Low Surface Expression and Intracellular Retention of the Patr-AL MHC Class I Molecule. J Immunol. 2015 Oct 15; 195(8):3725-36. PMID: 26371256; PMCID: PMC4592827 [Available on 10/15/16].
      View in: PubMed, PubMed Central
    3. Brodsky F, Sosa RT, Ybe JA, O'Halloran TJ. Unconventional functions for clathrin, ESCRTs, and other endocytic regulators in the cytoskeleton, cell cycle, nucleus, and beyond: links to human disease. Cold Spring Harb Perspect Biol. 2014 Sep; 6(9):a017004. PMID: 25183831.
      View in: PubMed
    4. Majeed SR, Vasudevan L, Chen CY, Luo Y, Torres JA, Evans TM, Sharkey A, Foraker AB, Wong NM, Esk C, Freeman TA, Moffett A, Keen JH, Brodsky F. Clathrin light chains are required for the gyrating-clathrin recycling pathway and thereby promote cell migration. Nat Commun. 2014; 5:3891. PMID: 24852344; PMCID: PMC4050264.
      View in: PubMed, PubMed Central
    5. Vassilopoulos S, Gentil C, Lainé J, Buclez PO, Franck A, Ferry A, Précigout G, Roth R, Heuser JE, Brodsky F, Garcia L, Bonne G, Voit T, Piétri-Rouxel F, Bitoun M. Actin scaffolding by clathrin heavy chain is required for skeletal muscle sarcomere organization. J Cell Biol. 2014 May 12; 205(3):377-93. PMID: 24798732; PMCID: PMC4018784.
      View in: PubMed, PubMed Central
    6. Sullivan CS, Scheib JL, Ma Z, Dang RP, Schafer JM, Hickman FE, Brodsky F, Ravichandran KS, Carter BD. The adaptor protein GULP promotes Jedi-1-mediated phagocytosis through a clathrin-dependent mechanism. Mol Biol Cell. 2014 Jun 15; 25(12):1925-36. PMID: 24743597; PMCID: PMC4055271.
      View in: PubMed, PubMed Central
    7. Asensio CS, Sirkis DW, Maas JW, Egami K, To TL, Brodsky F, Shu X, Cheng Y, Edwards RH. Self-assembly of VPS41 promotes sorting required for biogenesis of the regulated secretory pathway. Dev Cell. 2013 Nov 25; 27(4):425-37. PMID: 24210660; PMCID: PMC3974617.
      View in: PubMed, PubMed Central
    8. Hoshino S, Sakamoto K, Vassilopoulos S, Camus SM, Griffin CA, Esk C, Torres JA, Ohkoshi N, Ishii A, Tamaoka A, Funke BH, Kucherlapati R, Margeta M, Rando TA, Brodsky F. The CHC22 clathrin-GLUT4 transport pathway contributes to skeletal muscle regeneration. PLoS One. 2013; 8(10):e77787. PMID: 24204966; PMCID: PMC3813726.
      View in: PubMed, PubMed Central
    9. Brodsky F. Frances Brodsky: so you think you know all about clathrin? Interview by Caitlin Sedwick. J Cell Biol. 2013 Sep 16; 202(6):830-1. PMID: 24043697; PMCID: PMC3776341.
      View in: PubMed, PubMed Central
    10. Stachowiak JC, Brodsky F, Miller EA. A cost-benefit analysis of the physical mechanisms of membrane curvature. Nat Cell Biol. 2013 Sep; 15(9):1019-27. PMID: 23999615; PMCID: PMC3813008.
      View in: PubMed, PubMed Central
    11. Holmes BB, DeVos SL, Kfoury N, Li M, Jacks R, Yanamandra K, Ouidja MO, Brodsky F, Marasa J, Bagchi DP, Kotzbauer PT, Miller TM, Papy-Garcia D, Diamond MI. Heparan sulfate proteoglycans mediate internalization and propagation of specific proteopathic seeds. Proc Natl Acad Sci U S A. 2013 Aug 13; 110(33):E3138-47. PMID: 23898162; PMCID: PMC3746848.
      View in: PubMed, PubMed Central
    12. Young A, Stoilova-McPhie S, Rothnie A, Vallis Y, Harvey-Smith P, Ranson N, Kent H, Brodsky F, Pearse BM, Roseman A, Smith CJ. Hsc70-induced changes in clathrin-auxilin cage structure suggest a role for clathrin light chains in cage disassembly. Traffic. 2013 Sep; 14(9):987-96. PMID: 23710728; PMCID: PMC3776051.
      View in: PubMed, PubMed Central
    13. Hom N, Mehta KR, Chou T, Foraker AB, Brodsky F, Kirshenbaum K, Montclare JK. Anisotropic nanocrystal arrays organized on protein lattices formed by recombinant clathrin fragments. J Mater Chem. 2012 Dec 28; 22(44):23335-23339. PMID: 23836951.
      View in: PubMed
    14. Bonazzi M, Kühbacher A, Toledo-Arana A, Mallet A, Vasudevan L, Pizarro-Cerdá J, Brodsky F, Cossart P. A common clathrin-mediated machinery co-ordinates cell-cell adhesion and bacterial internalization. Traffic. 2012 Dec; 13(12):1653-66. PMID: 22984946; PMCID: PMC3760411.
      View in: PubMed, PubMed Central
    15. Foraker AB, Camus SM, Evans TM, Majeed SR, Chen CY, Taner SB, Corrêa IR, Doxsey SJ, Brodsky F. Clathrin promotes centrosome integrity in early mitosis through stabilization of centrosomal ch-TOG. J Cell Biol. 2012 Aug 20; 198(4):591-605. PMID: 22891263; PMCID: PMC3514040.
      View in: PubMed, PubMed Central
    16. Brodsky F. Diversity of clathrin function: new tricks for an old protein. Annu Rev Cell Dev Biol. 2012; 28:309-36. PMID: 22831640.
      View in: PubMed
    17. Brodsky F, Thattai M, Mayor S. Evolutionary cell biology: Lessons from diversity. Nat Cell Biol. 2012 Jul; 14(7):651. PMID: 22743706.
      View in: PubMed
    18. Bonazzi M, Vasudevan L, Mallet A, Sachse M, Sartori A, Prevost MC, Roberts A, Taner SB, Wilbur JD, Brodsky F, Cossart P. Clathrin phosphorylation is required for actin recruitment at sites of bacterial adhesion and internalization. J Cell Biol. 2011 Oct 31; 195(3):525-36. PMID: 22042622; PMCID: PMC3206339.
      View in: PubMed, PubMed Central
    19. Stoddart A, Tennant TR, Fernald AA, Anastasi J, Brodsky F, Le Beau MM. The clathrin-binding domain of CALM-AF10 alters the phenotype of myeloid neoplasms in mice. Oncogene. 2012 Jan 26; 31(4):494-506. PMID: 21706055; PMCID: PMC3204175.
      View in: PubMed, PubMed Central
    20. Shieh JC, Schaar BT, Srinivasan K, Brodsky F, McConnell SK. Endocytosis regulates cell soma translocation and the distribution of adhesion proteins in migrating neurons. PLoS One. 2011; 6(3):e17802. PMID: 21445347; PMCID: PMC3062553.
      View in: PubMed, PubMed Central
    21. Taner SB, Pando MJ, Roberts A, Schellekens J, Marsh SG, Malmberg KJ, Parham P, Brodsky F. Interactions of NK cell receptor KIR3DL1*004 with chaperones and conformation-specific antibody reveal a functional folded state as well as predominant intracellular retention. J Immunol. 2011 Jan 1; 186(1):62-72. PMID: 21115737; PMCID: PMC3129036.
      View in: PubMed, PubMed Central
    22. Wilbur JD, Hwang PK, Ybe JA, Lane M, Sellers BD, Jacobson MP, Fletterick RJ, Brodsky F. Conformation switching of clathrin light chain regulates clathrin lattice assembly. Dev Cell. 2010 May 18; 18(5):841-8. PMID: 20493816; PMCID: PMC2975025.
      View in: PubMed, PubMed Central
    23. Wilbur JD, Hwang PK, Brodsky F, Fletterick RJ. Accommodation of structural rearrangements in the huntingtin-interacting protein 1 coiled-coil domain. Acta Crystallogr D Biol Crystallogr. 2010 Mar; 66(Pt 3):314-8. PMID: 20179344; PMCID: PMC2827350.
      View in: PubMed, PubMed Central
    24. Esk C, Chen CY, Johannes L, Brodsky F. The clathrin heavy chain isoform CHC22 functions in a novel endosomal sorting step. J Cell Biol. 2010 Jan 11; 188(1):131-44. PMID: 20065094; PMCID: PMC2812854.
      View in: PubMed, PubMed Central
    25. Vassilopoulos S, Esk C, Hoshino S, Brodsky F. [The CHC22 human clathrin heavy chain isoform, intracellular traffic of the glucose transporter GLUT4, and type 2 diabetes]. Med Sci (Paris). 2010 Jan; 26(1):33-6. PMID: 20132771.
      View in: PubMed
    26. Sharma D, Bastard K, Guethlein LA, Norman PJ, Yawata N, Yawata M, Pando M, Thananchai H, Dong T, Rowland-Jones S, Brodsky F, Parham P. Dimorphic motifs in D0 and D1+D2 domains of killer cell Ig-like receptor 3DL1 combine to form receptors with high, moderate, and no avidity for the complex of a peptide derived from HIV and HLA-A*2402. J Immunol. 2009 Oct 1; 183(7):4569-82. PMID: 19752231; PMCID: PMC2827337.
      View in: PubMed, PubMed Central
    27. Vassilopoulos S, Esk C, Hoshino S, Funke BH, Chen CY, Plocik AM, Wright WE, Kucherlapati R, Brodsky F. A role for the CHC22 clathrin heavy-chain isoform in human glucose metabolism. Science. 2009 May 29; 324(5931):1192-6. PMID: 19478182; PMCID: PMC2975026.
      View in: PubMed, PubMed Central
    28. Wilbur JD, Chen CY, Manalo V, Hwang PK, Fletterick RJ, Brodsky F. Actin binding by Hip1 (huntingtin-interacting protein 1) and Hip1R (Hip1-related protein) is regulated by clathrin light chain. J Biol Chem. 2008 Nov 21; 283(47):32870-9. PMID: 18790740; PMCID: PMC2583295.
      View in: PubMed, PubMed Central
    29. Kirkham M, Nixon SJ, Howes MT, Abi-Rached L, Wakeham DE, Hanzal-Bayer M, Ferguson C, Hill MM, Fernandez-Rojo M, Brown DA, Hancock JF, Brodsky F, Parton RG. Evolutionary analysis and molecular dissection of caveola biogenesis. J Cell Sci. 2008 Jun 15; 121(Pt 12):2075-86. PMID: 18505796.
      View in: PubMed
    30. Sowinski S, Jolly C, Berninghausen O, Purbhoo MA, Chauveau A, Köhler K, Oddos S, Eissmann P, Brodsky F, Hopkins C, Onfelt B, Sattentau Q, Davis DM. Membrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission. Nat Cell Biol. 2008 Feb; 10(2):211-9. PMID: 18193035.
      View in: PubMed
    31. Knuehl C, Chen CY, Manalo V, Hwang PK, Ota N, Brodsky F. Novel binding sites on clathrin and adaptors regulate distinct aspects of coat assembly. Traffic. 2006 Dec; 7(12):1688-700. PMID: 17052248.
      View in: PubMed
    32. Chen TT, Li L, Chung DH, Allen CD, Torti SV, Torti FM, Cyster JG, Chen CY, Brodsky F, Niemi EC, Nakamura MC, Seaman WE, Daws MR. TIM-2 is expressed on B cells and in liver and kidney and is a receptor for H-ferritin endocytosis. J Exp Med. 2005 Oct 3; 202(7):955-65. PMID: 16203866; PMCID: PMC2213179.
      View in: PubMed, PubMed Central
    33. Wakeham DE, Abi-Rached L, Towler MC, Wilbur JD, Parham P, Brodsky F. Clathrin heavy and light chain isoforms originated by independent mechanisms of gene duplication during chordate evolution. Proc Natl Acad Sci U S A. 2005 May 17; 102(20):7209-14. PMID: 15883369; PMCID: PMC1091751.
      View in: PubMed, PubMed Central
    34. Wilbur JD, Hwang PK, Brodsky F. New faces of the familiar clathrin lattice. Traffic. 2005 Apr; 6(4):346-50. PMID: 15752139.
      View in: PubMed
    35. Stoddart A, Jackson AP, Brodsky F. Plasticity of B cell receptor internalization upon conditional depletion of clathrin. Mol Biol Cell. 2005 May; 16(5):2339-48. PMID: 15716350; PMCID: PMC1087239.
      View in: PubMed, PubMed Central
    36. Brodsky F. Cell biology: clathrin's Achilles' ankle. Nature. 2004 Dec 2; 432(7017):568-9. PMID: 15577897.
      View in: PubMed
    37. Chen CY, Brodsky F. Huntingtin-interacting protein 1 (Hip1) and Hip1-related protein (Hip1R) bind the conserved sequence of clathrin light chains and thereby influence clathrin assembly in vitro and actin distribution in vivo. J Biol Chem. 2005 Feb 18; 280(7):6109-17. PMID: 15533940.
      View in: PubMed
    38. Niiro H, Allam A, Stoddart A, Brodsky F, Marshall AJ, Clark EA. The B lymphocyte adaptor molecule of 32 kilodaltons (Bam32) regulates B cell antigen receptor internalization. J Immunol. 2004 Nov 1; 173(9):5601-9. PMID: 15494510.
      View in: PubMed
    39. Towler MC, Gleeson PA, Hoshino S, Rahkila P, Manalo V, Ohkoshi N, Ordahl C, Parton RG, Brodsky F. Clathrin isoform CHC22, a component of neuromuscular and myotendinous junctions, binds sorting nexin 5 and has increased expression during myogenesis and muscle regeneration. Mol Biol Cell. 2004 Jul; 15(7):3181-95. PMID: 15133132; PMCID: PMC452575.
      View in: PubMed, PubMed Central
    40. Crotzer VL, Mabardy AS, Weiss A, Brodsky F. T cell receptor engagement leads to phosphorylation of clathrin heavy chain during receptor internalization. J Exp Med. 2004 Apr 5; 199(7):981-91. PMID: 15067034; PMCID: PMC2211883.
      View in: PubMed, PubMed Central
    41. Towler MC, Kaufman SJ, Brodsky F. Membrane traffic in skeletal muscle. Traffic. 2004 Mar; 5(3):129-39. PMID: 15086789.
      View in: PubMed
    42. Wakeham DE, Chen CY, Greene B, Hwang PK, Brodsky F. Clathrin self-assembly involves coordinated weak interactions favorable for cellular regulation. EMBO J. 2003 Oct 1; 22(19):4980-90. PMID: 14517237; PMCID: PMC204494.
      View in: PubMed, PubMed Central
    43. Knuehl C, Brodsky F. The long and short of adaptor appendages. Nat Struct Biol. 2003 Aug; 10(8):580-2. PMID: 12886287.
      View in: PubMed
    44. Chen CY, Reese ML, Hwang PK, Ota N, Agard D, Brodsky F. Clathrin light and heavy chain interface: alpha-helix binding superhelix loops via critical tryptophans. EMBO J. 2002 Nov 15; 21(22):6072-82. PMID: 12426379; PMCID: PMC137186.
      View in: PubMed, PubMed Central
    45. Stoddart A, Dykstra ML, Brown BK, Song W, Pierce SK, Brodsky F. Lipid rafts unite signaling cascades with clathrin to regulate BCR internalization. Immunity. 2002 Oct; 17(4):451-62. PMID: 12387739.
      View in: PubMed
    46. Yang D, Buchholz F, Huang Z, Goga A, Chen CY, Brodsky F, Bishop JM. Short RNA duplexes produced by hydrolysis with Escherichia coli RNase III mediate effective RNA interference in mammalian cells. Proc Natl Acad Sci U S A. 2002 Jul 23; 99(15):9942-7. PMID: 12096193; PMCID: PMC126604.
      View in: PubMed, PubMed Central
    47. Bennett EM, Chen CY, Engqvist-Goldstein AE, Drubin DG, Brodsky F. Clathrin hub expression dissociates the actin-binding protein Hip1R from coated pits and disrupts their alignment with the actin cytoskeleton. Traffic. 2001 Nov; 2(11):851-8. PMID: 11733052.
      View in: PubMed
    48. Bennett EM, Lin SX, Towler MC, Maxfield FR, Brodsky F. Clathrin hub expression affects early endosome distribution with minimal impact on receptor sorting and recycling. Mol Biol Cell. 2001 Sep; 12(9):2790-9. PMID: 11553717; PMCID: PMC59713.
      View in: PubMed, PubMed Central
    49. Molinete M, Dupuis S, Brodsky F, Halban PA. Role of clathrin in the regulated secretory pathway of pancreatic beta-cells. J Cell Sci. 2001 Aug; 114(Pt 16):3059-66. PMID: 11686308.
      View in: PubMed
    50. Liu SH, Towler MC, Chen E, Chen CY, Song W, Apodaca G, Brodsky F. A novel clathrin homolog that co-distributes with cytoskeletal components functions in the trans-Golgi network. EMBO J. 2001 Jan 15; 20(1-2):272-84. PMID: 11226177; PMCID: PMC140205.
      View in: PubMed, PubMed Central
    51. Brodsky F, Chen CY, Knuehl C, Towler MC, Wakeham DE. Biological basket weaving: formation and function of clathrin-coated vesicles. Annu Rev Cell Dev Biol. 2001; 17:517-68. PMID: 11687498.
      View in: PubMed
    52. Beattie EC, Howe CL, Wilde A, Brodsky F, Mobley WC. NGF signals through TrkA to increase clathrin at the plasma membrane and enhance clathrin-mediated membrane trafficking. J Neurosci. 2000 Oct 1; 20(19):7325-33. PMID: 11007890.
      View in: PubMed
    53. Ybe JA, Wakeham DE, Brodsky F, Hwang PK. Molecular structures of proteins involved in vesicle fusion. Traffic. 2000 Jun; 1(6):474-9. PMID: 11208133.
      View in: PubMed
    54. Wakeham DE, Ybe JA, Brodsky F, Hwang PK. Molecular structures of proteins involved in vesicle coat formation. Traffic. 2000 May; 1(5):393-8. PMID: 11208125.
      View in: PubMed
    55. Jahn R, Brodsky F. Transport of proteins and membrane in eukaryotic cells (Göttingen, September 29-October 1, 1999). Traffic. 2000 Feb; 1(2):187-8. PMID: 11208099.
      View in: PubMed
    56. Greene B, Liu SH, Wilde A, Brodsky F. Complete reconstitution of clathrin basket formation with recombinant protein fragments: adaptor control of clathrin self-assembly. Traffic. 2000 Jan; 1(1):69-75. PMID: 11208061.
      View in: PubMed
    57. Altschuler Y, Liu S, Katz L, Tang K, Hardy S, Brodsky F, Apodaca G, Mostov K. ADP-ribosylation factor 6 and endocytosis at the apical surface of Madin-Darby canine kidney cells. J Cell Biol. 1999 Oct 4; 147(1):7-12. PMID: 10508850; PMCID: PMC2164974.
      View in: PubMed, PubMed Central
    58. Ybe JA, Brodsky F, Hofmann K, Lin K, Liu SH, Chen L, Earnest TN, Fletterick RJ, Hwang PK. Clathrin self-assembly is mediated by a tandemly repeated superhelix. Nature. 1999 May 27; 399(6734):371-5. PMID: 10360576.
      View in: PubMed
    59. Bennett EM, Bennink JR, Yewdell JW, Brodsky F. Cutting edge: adenovirus E19 has two mechanisms for affecting class I MHC expression. J Immunol. 1999 May 1; 162(9):5049-52. PMID: 10227971.
      View in: PubMed
    60. Brodsky F. Stealth, sabotage and exploitation. Immunol Rev. 1999 Apr; 168:5-11. PMID: 10399060.
      View in: PubMed
    61. Brodsky F, Lem L, Solache A, Bennett EM. Human pathogen subversion of antigen presentation. Immunol Rev. 1999 Apr; 168:199-215. PMID: 10399076.
      View in: PubMed
    62. Wilde A, Beattie EC, Lem L, Riethof DA, Liu SH, Mobley WC, Soriano P, Brodsky F. EGF receptor signaling stimulates SRC kinase phosphorylation of clathrin, influencing clathrin redistribution and EGF uptake. Cell. 1999 Mar 5; 96(5):677-87. PMID: 10089883.
      View in: PubMed
    63. Lem L, Riethof DA, Scidmore-Carlson M, Griffiths GM, Hackstadt T, Brodsky F. Enhanced interaction of HLA-DM with HLA-DR in enlarged vacuoles of hereditary and infectious lysosomal diseases. J Immunol. 1999 Jan 1; 162(1):523-32. PMID: 9886429.
      View in: PubMed
    64. Ybe JA, Greene B, Liu SH, Pley U, Parham P, Brodsky F. Clathrin self-assembly is regulated by three light-chain residues controlling the formation of critical salt bridges. EMBO J. 1998 Aug 10; 17(5):1297-303. PMID: 9482727; PMCID: PMC1170478.
      View in: PubMed, PubMed Central
    65. Lu X, Yu H, Liu SH, Brodsky F, Peterlin BM. Interactions between HIV1 Nef and vacuolar ATPase facilitate the internalization of CD4. Immunity. 1998 May; 8(5):647-56. PMID: 9620685.
      View in: PubMed
    66. Bursztajn S, Vincent S, Brodsky F, Benes F, Morris SA. A novel AP180-related protein in vesicles that concentrate at acetylcholine receptor clusters. J Cell Biochem. 1998 Mar 15; 68(4):457-71. PMID: 9493909.
      View in: PubMed
    67. Liu SH, Marks MS, Brodsky F. A dominant-negative clathrin mutant differentially affects trafficking of molecules with distinct sorting motifs in the class II major histocompatibility complex (MHC) pathway. J Cell Biol. 1998 Mar 9; 140(5):1023-37. PMID: 9490717; PMCID: PMC2132696.
      View in: PubMed, PubMed Central
    68. Brodsky F. New fashions in vesicle coats. Trends Cell Biol. 1997 May; 7(5):175-9. PMID: 17708937.
      View in: PubMed
    69. Bresnahan PA, Barber LD, Brodsky F. Localization of class I histocompatibility molecule assembly by subfractionation of the early secretory pathway. Hum Immunol. 1997 Apr 1; 53(2):129-39. PMID: 9129970.
      View in: PubMed
    70. Wilde A, Brodsky F, Boll W. Clathrin assembly: phosphorylation and peptides provide new tools. Trends Cell Biol. 1997 Feb; 7(2):47. PMID: 17708900.
      View in: PubMed
    71. Mallet WG, Brodsky F. A membrane-associated protein complex with selective binding to the clathrin coat adaptor AP1. J Cell Sci. 1996 Dec; 109 ( Pt 13):3059-68. PMID: 9004040.
      View in: PubMed
    72. Wilde A, Brodsky F. In vivo phosphorylation of adaptors regulates their interaction with clathrin. J Cell Biol. 1996 Nov; 135(3):635-45. PMID: 8909539; PMCID: PMC2121074.
      View in: PubMed, PubMed Central
    73. Brodsky F, Lem L, Bresnahan PA. Antigen processing and presentation. Tissue Antigens. 1996 Jun; 47(6):464-71. PMID: 8813734.
      View in: PubMed
    74. Liu SH, Wong ML, Craik CS, Brodsky F. Regulation of clathrin assembly and trimerization defined using recombinant triskelion hubs. Cell. 1995 Oct 20; 83(2):257-67. PMID: 7585943.
      View in: PubMed
    75. Pley UM, Hill BL, Alibert C, Brodsky F, Parham P. The interaction of calmodulin with clathrin-coated vesicles, triskelions, and light chains. Localization of a binding site. J Biol Chem. 1995 Feb 3; 270(5):2395-402. PMID: 7836475.
      View in: PubMed
    76. Zia-Amirhosseini PZ, Harris RZ, Brodsky F, Benet LZ. Hypersensitivity to nonsteroidal anti-inflammatory drugs. Nat Med. 1995 Jan; 1(1):2-4. PMID: 7584944.
      View in: PubMed
    77. Acton SL, Wong DH, Parham P, Brodsky F, Jackson AP. Alteration of clathrin light chain expression by transfection and gene disruption. Mol Biol Cell. 1993 Jun; 4(6):647-60. PMID: 8374173; PMCID: PMC300971.
      View in: PubMed, PubMed Central
    78. Chang MP, Mallet WG, Mostov KE, Brodsky F. Adaptor self-aggregation, adaptor-receptor recognition and binding of alpha-adaptin subunits to the plasma membrane contribute to recruitment of adaptor (AP2) components of clathrin-coated pits. EMBO J. 1993 May; 12(5):2169-80. PMID: 8491205; PMCID: PMC413438.
      View in: PubMed, PubMed Central
    79. Beck KA, Chang M, Brodsky F, Keen JH. Clathrin assembly protein AP-2 induces aggregation of membrane vesicles: a possible role for AP-2 in endosome formation. J Cell Biol. 1992 Nov; 119(4):787-96. PMID: 1358896; PMCID: PMC2289704.
      View in: PubMed, PubMed Central
    80. Hille A, Klumperman J, Geuze HJ, Peters C, Brodsky F, von Figura K. Lysosomal acid phosphatase is internalized via clathrin-coated pits. Eur J Cell Biol. 1992 Oct; 59(1):106-15. PMID: 1468434.
      View in: PubMed
    81. Wong DH, Brodsky F. 100-kD proteins of Golgi- and trans-Golgi network-associated coated vesicles have related but distinct membrane binding properties. J Cell Biol. 1992 Jun; 117(6):1171-9. PMID: 1607381; PMCID: PMC2289504.
      View in: PubMed, PubMed Central
    82. Koppelman B, Zimmerman DL, Walter P, Brodsky F. Evidence for peptide transport across microsomal membranes. Proc Natl Acad Sci U S A. 1992 May 1; 89(9):3908-12. PMID: 1570312; PMCID: PMC525600.
      View in: PubMed, PubMed Central
    83. Brodsky F. Antigen processing and presentation: close encounters in the endocytic pathway. Trends Cell Biol. 1992 Apr; 2(4):109-15. PMID: 14732015.
      View in: PubMed
    84. Näthke IS, Heuser J, Lupas A, Stock J, Turck CW, Brodsky F. Folding and trimerization of clathrin subunits at the triskelion hub. Cell. 1992 Mar 6; 68(5):899-910. PMID: 1547490.
      View in: PubMed
    85. Brodsky F. What's the score? Nature. 1991 Jul 25; 352(6333):288-9. PMID: 1677162.
      View in: PubMed
    86. Brodsky F. Intracellular routes for antigen processing and presentation. Res Immunol. 1991 Jun-Aug; 142(5-6):453-8. PMID: 1754720.
      View in: PubMed
    87. Brodsky F, Hill BL, Acton SL, Näthke I, Wong DH, Ponnambalam S, Parham P. Clathrin light chains: arrays of protein motifs that regulate coated-vesicle dynamics. Trends Biochem Sci. 1991 Jun; 16(6):208-13. PMID: 1909824.
      View in: PubMed
    88. VanBuskirk AM, DeNagel DC, Guagliardi LE, Brodsky F, Pierce SK. Cellular and subcellular distribution of PBP72/74, a peptide-binding protein that plays a role in antigen processing. J Immunol. 1991 Jan 15; 146(2):500-6. PMID: 1987275.
      View in: PubMed
    89. Brodsky F, Guagliardi LE. The cell biology of antigen processing and presentation. Annu Rev Immunol. 1991; 9:707-44. PMID: 1910692.
      View in: PubMed
    90. Brodsky F. Immunology. The invariant dating service. Nature. 1990 Dec 13; 348(6302):581-2. PMID: 2250712.
      View in: PubMed
    91. Näthke I, Hill BL, Parham P, Brodsky F. The calcium-binding site of clathrin light chains. J Biol Chem. 1990 Oct 25; 265(30):18621-7. PMID: 2211724.
      View in: PubMed
    92. Acton SL, Brodsky F. Predominance of clathrin light chain LCb correlates with the presence of a regulated secretory pathway. J Cell Biol. 1990 Oct; 111(4):1419-26. PMID: 2211818; PMCID: PMC2116225.
      View in: PubMed, PubMed Central
    93. Mahaffey DT, Peeler JS, Brodsky F, Anderson RG. Clathrin-coated pits contain an integral membrane protein that binds the AP-2 subunit with high affinity. J Biol Chem. 1990 Sep 25; 265(27):16514-20. PMID: 1975814.
      View in: PubMed
    94. Wong DH, Ignatius MJ, Parosky G, Parham P, Trojanowski JQ, Brodsky F. Neuron-specific expression of high-molecular-weight clathrin light chain. J Neurosci. 1990 Sep; 10(9):3025-31. PMID: 2204686.
      View in: PubMed
    95. Draper RK, Goda Y, Brodsky F, Pfeffer SR. Antibodies to clathrin inhibit endocytosis but not recycling to the trans Golgi network in vitro. Science. 1990 Jun 22; 248(4962):1539-41. PMID: 2163108.
      View in: PubMed
    96. Guagliardi LE, Koppelman B, Blum JS, Marks MS, Cresswell P, Brodsky F. Co-localization of molecules involved in antigen processing and presentation in an early endocytic compartment. Nature. 1990 Jan 11; 343(6254):133-9. PMID: 2404209.
      View in: PubMed
    97. Chin DJ, Straubinger RM, Acton S, Näthke I, Brodsky F. 100-kDa polypeptides in peripheral clathrin-coated vesicles are required for receptor-mediated endocytosis. Proc Natl Acad Sci U S A. 1989 Dec; 86(23):9289-93. PMID: 2574457; PMCID: PMC298480.
      View in: PubMed, PubMed Central
    98. Szöllösi J, Damjanovich S, Balàzs M, Nagy P, Trón L, Fulwyler MJ, Brodsky F. Physical association between MHC class I and class II molecules detected on the cell surface by flow cytometric energy transfer. J Immunol. 1989 Jul 1; 143(1):208-13. PMID: 2732468.
      View in: PubMed
    99. Mahaffey DT, Moore MS, Brodsky F, Anderson RG. Coat proteins isolated from clathrin coated vesicles can assemble into coated pits. J Cell Biol. 1989 May; 108(5):1615-24. PMID: 2565904; PMCID: PMC2115561.
      View in: PubMed, PubMed Central
    100. Parham P, Brodsky F, Drickamer K. The occurrence of disulphide bonds in purified clathrin light chains. Biochem J. 1989 Feb 1; 257(3):775-81. PMID: 2930486; PMCID: PMC1135655.
      View in: PubMed, PubMed Central
    101. Brodsky F, Koppelman B, Blum JS, Marks MS, Cresswell P, Guagliardi L. Intracellular colocalization of molecules involved in antigen processing and presentation by B cells. Cold Spring Harb Symp Quant Biol. 1989; 54 Pt 1:319-31. PMID: 2700939.
      View in: PubMed
    102. Brodsky F. Living with clathrin: its role in intracellular membrane traffic. Science. 1988 Dec 9; 242(4884):1396-402. PMID: 2904698.
      View in: PubMed
    103. Robbins PA, Maino VC, Warner NL, Brodsky F. Activated T cells and monocytes have characteristic patterns of class II antigen expression. J Immunol. 1988 Aug 15; 141(4):1281-7. PMID: 3135327.
      View in: PubMed
    104. Hill BL, Drickamer K, Brodsky F, Parham P. Identification of the phosphorylation sites of clathrin light chain LCb. J Biol Chem. 1988 Apr 25; 263(12):5499-501. PMID: 3128543.
      View in: PubMed
    105. Brodsky F. Analysis of coated vesicle structure and function using anti-clathrin monoclonal antibodies. Prog Clin Biol Res. 1988; 270:141-52. PMID: 3413156.
      View in: PubMed
    106. Metzker A, Brodsky F. Traumatic plantar urticaria--an unrecognized entity? J Am Acad Dermatol. 1988 Jan; 18(1 Pt 1):144-6. PMID: 3346400.
      View in: PubMed
    107. Brodsky F. Monoclonal antibodies as magic bullets. Pharm Res. 1988 Jan; 5(1):1-9. PMID: 3072552.
      View in: PubMed
    108. Blank GS, Brodsky F. Clathrin assembly involves a light chain-binding region. J Cell Biol. 1987 Nov; 105(5):2011-9. PMID: 2445759; PMCID: PMC2114853.
      View in: PubMed, PubMed Central
    109. Sadée W, Yu VC, Richards ML, Preis PN, Schwab MR, Brodsky F, Biedler JL. Expression of neurotransmitter receptors and myc protooncogenes in subclones of a human neuroblastoma cell line. Cancer Res. 1987 Oct 1; 47(19):5207-12. PMID: 3040240.
      View in: PubMed
    110. Doxsey SJ, Brodsky F, Blank GS, Helenius A. Inhibition of endocytosis by anti-clathrin antibodies. Cell. 1987 Jul 31; 50(3):453-63. PMID: 3111717.
      View in: PubMed
    111. Moore MS, Mahaffey DT, Brodsky F, Anderson RG. Assembly of clathrin-coated pits onto purified plasma membranes. Science. 1987 May 1; 236(4801):558-63. PMID: 2883727.
      View in: PubMed
    112. Robbins PA, Evans EL, Ding AH, Warner NL, Brodsky F. Monoclonal antibodies that distinguish between class II antigens (HLA-DP, DQ, and DR) in 14 haplotypes. Hum Immunol. 1987 Apr; 18(4):301-13. PMID: 3494718.
      View in: PubMed
    113. Clayberger C, Krensky AM, McIntyre BW, Koller TD, Parham P, Brodsky F, Linn DJ, Evans EL. Identification and characterization of two novel lymphocyte function-associated antigens, L24 and L25. J Immunol. 1987 Mar 1; 138(5):1510-4. PMID: 3492556.
      View in: PubMed
    114. Brodsky F, Galloway CJ, Blank GS, Jackson AP, Seow HF, Drickamer K, Parham P. Localization of clathrin light-chain sequences mediating heavy-chain binding and coated vesicle diversity. Nature. 1987 Mar 12-18; 326(6109):203-5. PMID: 2434865.
      View in: PubMed
    115. Blank GS, Brodsky F. Site-specific disruption of clathrin assembly produces novel structures. EMBO J. 1986 Sep; 5(9):2087-95. PMID: 3096714; PMCID: PMC1167086.
      View in: PubMed, PubMed Central
    116. Radka SF, Charron DJ, Brodsky F. Class II molecules of the major histocompatibility complex considered as differentiation markers. Hum Immunol. 1986 Aug; 16(4):390-400. PMID: 2428784.
      View in: PubMed
    117. Brodsky F. Clathrin structure characterized with monoclonal antibodies. I. Analysis of multiple antigenic sites. J Cell Biol. 1985 Dec; 101(6):2047-54. PMID: 2415533; PMCID: PMC2114023.
      View in: PubMed, PubMed Central
    118. Brodsky F. Clathrin structure characterized with monoclonal antibodies. II. Identification of in vivo forms of clathrin. J Cell Biol. 1985 Dec; 101(6):2055-62. PMID: 4066749; PMCID: PMC2114020.
      View in: PubMed, PubMed Central
    119. Brodsky F. The intracellular traffic of immunologically active molecules. Immunol Today. 1984 Dec; 5(12):350-7. PMID: 25290979.
      View in: PubMed
    120. Chen YX, Evans RL, Pollack MS, Lanier LL, Phillips JH, Rousso C, Warner NL, Brodsky F. Characterization and expression of the HLA-DC antigens defined by anti-Leu 10. Hum Immunol. 1984 Aug; 10(4):221-35. PMID: 6332101.
      View in: PubMed
    121. Holmes N, Biermann JS, Brodsky F, Bharucha D, Parham P. Comparison of the primary structures of clathrin light chains from bovine brain and adrenal gland by peptide mapping. EMBO J. 1984 Jul; 3(7):1621-7. PMID: 6745244; PMCID: PMC557568.
      View in: PubMed, PubMed Central
    122. Brodsky F. A matrix approach to human class II histocompatibility antigens: reactions of four monoclonal antibodies with the products of nine haplotypes. Immunogenetics. 1984; 19(3):179-94. PMID: 6200433.
      View in: PubMed
    123. Brodsky F, Parham P. Polymorphism in clathrin light chains from different tissues. J Mol Biol. 1983 Jun 15; 167(1):197-204. PMID: 6864801.
      View in: PubMed
    124. Kirchhausen T, Harrison SC, Parham P, Brodsky F. Location and distribution of the light chains in clathrin trimers. Proc Natl Acad Sci U S A. 1983 May; 80(9):2481-5. PMID: 6189124; PMCID: PMC393849.
      View in: PubMed, PubMed Central
    125. Payne R, Brodsky F, Peterlin BM, Young LM. "Bare lymphocytes" without immunodeficiency. Hum Immunol. 1983 Apr; 6(4):219-27. PMID: 6573306.
      View in: PubMed
    126. Brodsky F, Holmes NJ, Parham P. Tropomyosin-like properties of clathrin light chains allow a rapid, high-yield purification. J Cell Biol. 1983 Mar; 96(3):911-4. PMID: 6833387; PMCID: PMC2112400.
      View in: PubMed, PubMed Central
    127. Parham P, Androlewicz MJ, Brodsky F, Holmes NJ, Ways JP. Monoclonal antibodies: purification, fragmentation and application to structural and functional studies of class I MHC antigens. J Immunol Methods. 1982 Sep 17; 53(2):133-73. PMID: 6183363.
      View in: PubMed
    128. Mason R, Austyn J, Brodsky F, Gordon S. Monoclonal antimacrophage antibodies: human pulmonary macrophages express HLA-DR (Ia-like) antigens in culture. Am Rev Respir Dis. 1982 May; 125(5):586-93. PMID: 6952778.
      View in: PubMed
    129. Brodsky F, Parham P. Monomorphic anti-HLA-A,B,C monoclonal antibodies detecting molecular subunits and combinatorial determinants. J Immunol. 1982 Jan; 128(1):129-35. PMID: 6172474.
      View in: PubMed
    130. Brodsky F, Parham P. Evolution of HLA antigenic determinants: species cross-reactions of monoclonal antibodies. Immunogenetics. 1982; 15(2):151-66. PMID: 6174442.
      View in: PubMed
    131. Parham P, Brodsky F. Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28. Hum Immunol. 1981 Dec; 3(4):277-99. PMID: 7035415.
      View in: PubMed
    132. Brodsky F, Stone WH, Parham P. Of cows and men: a comparative study of histocompatibility antigens. Hum Immunol. 1981 Oct; 3(2):143-52. PMID: 7035414.
      View in: PubMed
    133. McMichael AJ, Parham P, Rust N, Brodsky F. A monoclonal antibody that recognizes an antigenic determinant shared by HLA A2 and B17. Hum Immunol. 1980 Sep; 1(2):121-9. PMID: 6167544.
      View in: PubMed
    134. McMichael AJ, Parham P, Brodsky F, Pilch JR. Influenza virus-specific cytotoxic T lymphocytes recognize HLA-molecules. Blocking by monoclonal anti-HLA antibodies. J Exp Med. 1980 Aug 1; 152(2 Pt 2):195s-203s. PMID: 6967937.
      View in: PubMed
    135. Brodsky F, Parham P, Bodmer WF. Monoclonal antibodies to HLA--DRw determinants. Tissue Antigens. 1980 Jul; 16(1):30-48. PMID: 6162228.
      View in: PubMed
    136. Brodsky F, Bodmer WF, Parham P. Characterization of a monoclonal anti-beta 2-microglobulin antibody and its use in the genetic and biochemical analysis of major histocompatibility antigens. Eur J Immunol. 1979 Jul; 9(7):536-45. PMID: 91522.
      View in: PubMed
    137. Parham P, Sehgal PK, Brodsky F. Anti-HLA-A,B,C monoclonal antibodies with no alloantigenic specificity in humans define polymorphisms in other primate species. Nature. 1979 Jun 14; 279(5714):639-41. PMID: 88017.
      View in: PubMed
    138. Feuerman EJ, Brodsky F. Nonthrombocytopenic purpura induced by carbromal. Cutis. 1979 Apr; 23(4):489-90. PMID: 34502.
      View in: PubMed
    139. Brodsky F, Parham P, Barnstable CJ, Crumpton MJ, Bodmer WF. Monoclonal antibodies for analysis of the HLA system. Immunol Rev. 1979; 47:3-61. PMID: 95015.
      View in: PubMed
    140. Brodsky F, Alteras I, Feuerman EJ. [Opportunistic fungi in the sputum of patients with malignancy]. Harefuah. 1978 May 15; 94(10):317-9. PMID: 689513.
      View in: PubMed
    141. Brodsky F, Wray SH, Tabira T, de F Webster H. Preparation of whole mounts of rabbit retina for examination with the differential-interference microscope: a technique for morphological studies of CNS demyelination. Brain Res. 1977 Mar 18; 124(1):140-6. PMID: 321098.
      View in: PubMed
    Frances's Networks
    Concepts
    Derived automatically from this person's publications.
    See all (572) concept(s)
    _
    Co-Authors
    People in Profiles who have published with this person.
    See all (24) people
    _
    Related Authors
    People who share related concepts with this person.
    See all (60) people
    _
    Back to TOP