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Geeta Narlikar, PhD

Title(s)Professor, Biochemistry and Biophysics
SchoolSchool of Medicine
Phone415-514-0394
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    Collapse Research 
    Collapse Research Activities and Funding
    ATP-dependent and independent mechanisms of regulating chromatin states
    NIH/NIGMS R35GM127020May 1, 2018 - Apr 30, 2023
    Role: Principal Investigator
    Mechanistic dissection of HP1 mediated heterochromatin
    NIH/NIGMS R01GM108455May 1, 2014 - Apr 30, 2018
    Role: Principal Investigator
    Mechanistic Analysis of Chromatin Remodeling
    NIH/NIGMS R01GM073767Apr 1, 2005 - Nov 30, 2018
    Role: Principal Investigator
    Bio-Organic Biomedical Mass Spectrometry Resource
    NIH P41RR001614Mar 1, 1982 - May 31, 2015
    Role: Co-Investigator
    Cell Biology, Genetics, and Biochemistry Training Grant
    NIH/NIGMS T32GM007810Jul 1, 1979 - Jun 30, 2021
    Role: Principal Investigator

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    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. Sanulli S, Trnka MJ, Dharmarajan V, Tibble RW, Pascal BD, Burlingame AL, Griffin PR, Gross JD, Narlikar GJ. HP1 reshapes nucleosome core to promote phase separation of heterochromatin. Nature. 2019 11; 575(7782):390-394. PMID: 31618757.
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    2. Mir M, Bickmore W, Furlong EEM, Narlikar G. Chromatin topology, condensates and gene regulation: shifting paradigms or just a phase? Development. 2019 Sep 25; 146(19). PMID: 31554625.
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    3. Armache JP, Gamarra N, Johnson SL, Leonard JD, Wu S, Narlikar GJ, Cheng Y. Cryo-EM structures of remodeler-nucleosome intermediates suggest allosteric control through the nucleosome. Elife. 2019 06 18; 8. PMID: 31210637.
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    4. Gebala M, Johnson SL, Narlikar GJ, Herschlag D. Ion counting demonstrates a high electrostatic field generated by the nucleosome. Elife. 2019 06 11; 8. PMID: 31184587.
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    5. Geeta Narlikar, Serena Sanulli, John D. Gross, Patrick Griffin, Mike Trnka. The Role of Phase-Separation in Heterochromatin. Biophysical Journal. 2019 Feb 1; 116(3):161a.
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    6. Stoddard CI, Feng S, Campbell MG, Liu W, Wang H, Zhong X, Bernatavichute Y, Cheng Y, Jacobsen SE, Narlikar GJ. A Nucleosome Bridging Mechanism for Activation of a Maintenance DNA Methyltransferase. Mol Cell. 2019 01 03; 73(1):73-83.e6. PMID: 30415948.
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    7. Keenen MM, Larson AG, Narlikar GJ. Visualization and Quantitation of Phase-Separated Droplet Formation by Human HP1a. Methods Enzymol. 2018; 611:51-66. PMID: 30471698.
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    8. Larson AG, Narlikar GJ. The Role of Phase Separation in Heterochromatin Formation, Function, and Regulation. Biochemistry. 2018 05 01; 57(17):2540-2548. PMID: 29644850.
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    9. Gamarra N, Johnson SL, Trnka MJ, Burlingame AL, Narlikar GJ. The nucleosomal acidic patch relieves auto-inhibition by the ISWI remodeler SNF2h. Elife. 2018 04 17; 7. PMID: 29664398.
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    10. Zhou CY, Johnson SL, Lee LJ, Longhurst AD, Beckwith SL, Johnson MJ, Morrison AJ, Narlikar GJ. The Yeast INO80 Complex Operates as a Tunable DNA Length-Sensitive Switch to Regulate Nucleosome Sliding. Mol Cell. 2018 02 15; 69(4):677-688.e9. PMID: 29452642.
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    11. Madeline M. Keenen, Adam G. Larson, Geeta J. Narlikar, Sy Redding. Dissecting the Mechanism of HP1 Mediated Chromatin Compaction via Single Molecule DNA Curtains. Biophysical Journal. 2018 Feb 1; 114(3):30a.
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    12. Isaac RS, Sanulli S, Tibble R, Hornsby M, Ravalin M, Craik CS, Gross JD, Narlikar GJ. Biochemical Basis for Distinct Roles of the Heterochromatin Proteins Swi6 and Chp2. J Mol Biol. 2017 11 24; 429(23):3666-3677. PMID: 28942089.
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    13. Larson AG, Elnatan D, Keenen MM, Trnka MJ, Johnston JB, Burlingame AL, Agard DA, Redding S, Narlikar GJ. Liquid droplet formation by HP1a suggests a role for phase separation in heterochromatin. Nature. 2017 07 13; 547(7662):236-240. PMID: 28636604.
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    14. Zhou CY, Stoddard CI, Johnston JB, Trnka MJ, Echeverria I, Palovcak E, Sali A, Burlingame AL, Cheng Y, Narlikar GJ. Regulation of Rvb1/Rvb2 by a Domain within the INO80 Chromatin Remodeling Complex Implicates the Yeast Rvbs as Protein Assembly Chaperones. Cell Rep. 2017 06 06; 19(10):2033-2044. PMID: 28591576.
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    15. Sinha KK, Gross JD, Narlikar GJ. Distortion of histone octamer core promotes nucleosome mobilization by a chromatin remodeler. Science. 2017 01 20; 355(6322). PMID: 28104838.
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    16. Zhou CY, Johnson SL, Gamarra NI, Narlikar GJ. Mechanisms of ATP-Dependent Chromatin Remodeling Motors. Annu Rev Biophys. 2016 07 05; 45:153-81. PMID: 27391925.
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    17. Isaac RS, Jiang F, Doudna JA, Lim WA, Narlikar GJ, Almeida R. Nucleosome breathing and remodeling constrain CRISPR-Cas9 function. Elife. 2016 04 28; 5. PMID: 27130520.
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    18. Zhou CY, Narlikar GJ. Analysis of Nucleosome Sliding by ATP-Dependent Chromatin Remodeling Enzymes. Methods Enzymol. 2016; 573:119-35. PMID: 27372751.
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    19. Stephanie L. Johnson, Nathan I. Gamarra, Matthew J. Johnson, Geeta J. Narlikar. Single-Molecule Mechanistic Dissection of a Chromatin Remodeling Motor. Biophysical Journal. 2016 Feb 1; 110(3):67a-68a.
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    20. Zhao H, Ghirlando R, Alfonso C, Arisaka F, Attali I, Bain DL, Bakhtina MM, Becker DF, Bedwell GJ, Bekdemir A, Besong TM, Birck C, Brautigam CA, Brennerman W, Byron O, Bzowska A, Chaires JB, Chaton CT, Cölfen H, Connaghan KD, Crowley KA, Curth U, Daviter T, Dean WL, Díez AI, Ebel C, Eckert DM, Eisele LE, Eisenstein E, England P, Escalante C, Fagan JA, Fairman R, Finn RM, Fischle W, de la Torre JG, Gor J, Gustafsson H, Hall D, Harding SE, Cifre JG, Herr AB, Howell EE, Isaac RS, Jao SC, Jose D, Kim SJ, Kokona B, Kornblatt JA, Kosek D, Krayukhina E, Krzizike D, Kusznir EA, Kwon H, Larson A, Laue TM, Le Roy A, Leech AP, Lilie H, Luger K, Luque-Ortega JR, Ma J, May CA, Maynard EL, Modrak-Wojcik A, Mok YF, Mücke N, Nagel-Steger L, Narlikar GJ, Noda M, Nourse A, Obsil T, Park CK, Park JK, Pawelek PD, Perdue EE, Perkins SJ, Perugini MA, Peterson CL, Peverelli MG, Piszczek G, Prag G, Prevelige PE, Raynal BD, Rezabkova L, Richter K, Ringel AE, Rosenberg R, Rowe AJ, Rufer AC, Scott DJ, Seravalli JG, Solovyova AS, Song R, Staunton D, Stoddard C, Stott K, Strauss HM, Streicher WW, Sumida JP, Swygert SG, Szczepanowski RH, Tessmer I, Toth RT, Tripathy A, Uchiyama S, Uebel SF, Unzai S, Gruber AV, von Hippel PH, Wandrey C, Wang SH, Weitzel SE, Wielgus-Kutrowska B, Wolberger C, Wolff M, Wright E, Wu YS, Wubben JM, Schuck P. A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation. PLoS One. 2015; 10(5):e0126420. PMID: 25997164.
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    21. Leonard JD, Narlikar GJ. A nucleotide-driven switch regulates flanking DNA length sensing by a dimeric chromatin remodeler. Mol Cell. 2015 Mar 05; 57(5):850-859. PMID: 25684208.
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    22. Racki LR, Naber N, Pate E, Leonard JD, Cooke R, Narlikar GJ. The histone H4 tail regulates the conformation of the ATP-binding pocket in the SNF2h chromatin remodeling enzyme. J Mol Biol. 2014 May 15; 426(10):2034-44. PMID: 24607692.
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    23. Canzio D, Larson A, Narlikar GJ. Mechanisms of functional promiscuity by HP1 proteins. Trends Cell Biol. 2014 Jun; 24(6):377-86. PMID: 24618358.
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    24. John D. Leonard, Jean-Paul Armache, Nariman Naber, Shenping Wu, Edward Pate, Roger Cooke, Yifan Cheng, Geeta J. Narlikar. The Mechanism of Nucleosome Spacing by a Dimeric Chromatin Remodeling Enzyme. Biophysical Journal. 2014 Jan 1; 106(2):69a-70a.
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    25. Coral Y. Zhou, Geeta J. Narlikar. Mechanism of Nucleosome Remodeling by INO80 from S. Cerevisiae. Biophysical Journal. 2014 Jan 1; 106(2):76a.
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    26. Narlikar GJ, Sundaramoorthy R, Owen-Hughes T. Mechanisms and functions of ATP-dependent chromatin-remodeling enzymes. Cell. 2013 Aug 01; 154(3):490-503. PMID: 23911317.
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    27. Al-Sady B, Madhani HD, Narlikar GJ. Division of labor between the chromodomains of HP1 and Suv39 methylase enables coordination of heterochromatin spread. Mol Cell. 2013 Jul 11; 51(1):80-91. PMID: 23849629.
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    28. Shiau C, Trnka MJ, Bozicevic A, Ortiz Torres I, Al-Sady B, Burlingame AL, Narlikar GJ, Fujimori DG. Reconstitution of nucleosome demethylation and catalytic properties of a Jumonji histone demethylase. Chem Biol. 2013 Apr 18; 20(4):494-9. PMID: 23601638.
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    29. Canzio D, Liao M, Naber N, Pate E, Larson A, Wu S, Marina DB, Garcia JF, Madhani HD, Cooke R, Schuck P, Cheng Y, Narlikar GJ. A conformational switch in HP1 releases auto-inhibition to drive heterochromatin assembly. Nature. 2013 Apr 18; 496(7445):377-81. PMID: 23485968.
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    30. Rougemaille M, Braun S, Coyle S, Dumesic PA, Garcia JF, Isaac RS, Libri D, Narlikar GJ, Madhani HD. Ers1 links HP1 to RNAi. Proc Natl Acad Sci U S A. 2012 Jul 10; 109(28):11258-63. PMID: 22733737.
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    31. Shahian T, Narlikar GJ. Analysis of changes in nucleosome conformation using fluorescence resonance energy transfer. Methods Mol Biol. 2012; 833:337-49. PMID: 22183603.
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    32. Geeta Narlikar, John Leonard, Lisa Racki, Nariman Naber, Roger Cooke, Yifan Cheng. Mechanisms of Chromatin Remodeling Motors. Biophysical Journal. 2012 Jan 1; 102(3):428a.
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    33. Armache KJ, Garlick JD, Canzio D, Narlikar GJ, Kingston RE. Structural basis of silencing: Sir3 BAH domain in complex with a nucleosome at 3.0 Å resolution. Science. 2011 Nov 18; 334(6058):977-82. PMID: 22096199.
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    34. Charles GM, Chen C, Shih SC, Collins SR, Beltrao P, Zhang X, Sharma T, Tan S, Burlingame AL, Krogan NJ, Madhani HD, Narlikar GJ. Site-specific acetylation mark on an essential chromatin-remodeling complex promotes resistance to replication stress. Proc Natl Acad Sci U S A. 2011 Jun 28; 108(26):10620-5. PMID: 21673141.
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    35. Lisa Racki, Nariman Naber, Roger Cooke, Geeta Narlikar. EPR Spectroscopy Shows That the H4 Tail of the Nucleosome Influences the Nucleotide-Binding Pocket of Acf. Biophysical Journal. 2011 Feb 1; 100(3):69a.
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    36. Canzio D, Chang EY, Shankar S, Kuchenbecker KM, Simon MD, Madhani HD, Narlikar GJ, Al-Sady B. Chromodomain-mediated oligomerization of HP1 suggests a nucleosome-bridging mechanism for heterochromatin assembly. Mol Cell. 2011 Jan 07; 41(1):67-81. PMID: 21211724.
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    37. Rowe CE, Narlikar GJ. The ATP-dependent remodeler RSC transfers histone dimers and octamers through the rapid formation of an unstable encounter intermediate. Biochemistry. 2010 Nov 16; 49(45):9882-90. PMID: 20853842.
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    38. Narlikar GJ. A proposal for kinetic proof reading by ISWI family chromatin remodeling motors. Curr Opin Chem Biol. 2010 Oct; 14(5):660-5. PMID: 20833099.
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    39. Lisa R. Racki, Geeta J. Narlikar, Roger Cooke, Nariman Naber, Thomas J. Purcell, Janet G. Yang, Yifan Cheng, Ashley Acevedo, Peretz Partensky. The Chromatin-Remodeling Complex ACF Functions as a Dimeric Motor to Space Nucleosomes. Biophysical Journal. 2010 Jan 1; 98(3):476a.
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    40. Racki LR, Yang JG, Naber N, Partensky PD, Acevedo A, Purcell TJ, Cooke R, Cheng Y, Narlikar GJ. The chromatin remodeller ACF acts as a dimeric motor to space nucleosomes. Nature. 2009 Dec 24; 462(7276):1016-21. PMID: 20033039.
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    41. Blosser TR, Yang JG, Stone MD, Narlikar GJ, Zhuang X. Dynamics of nucleosome remodelling by individual ACF complexes. Nature. 2009 Dec 24; 462(7276):1022-7. PMID: 20033040.
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    42. Partensky PD, Narlikar GJ. Chromatin remodelers act globally, sequence positions nucleosomes locally. J Mol Biol. 2009 Aug 07; 391(1):12-25. PMID: 19450608.
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    43. Timothy R. Blosser, Michael D. Stone, Janet Yang, Geeta Narlikar, Xiaowei Zhuang. Nucleosome Sliding by ACF is Processive and Bidirectional. Biophysical Journal. 2009 Feb 1; 96(3):566a.
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    44. Chang EY, Ferreira H, Somers J, Nusinow DA, Owen-Hughes T, Narlikar GJ. MacroH2A allows ATP-dependent chromatin remodeling by SWI/SNF and ACF complexes but specifically reduces recruitment of SWI/SNF. Biochemistry. 2008 Dec 23; 47(51):13726-32. PMID: 19035833.
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    45. Madhani HD, Francis NJ, Kingston RE, Kornberg RD, Moazed D, Narlikar GJ, Panning B, Struhl K. Epigenomics: a roadmap, but to where? Science. 2008 Oct 03; 322(5898):43-4. PMID: 18832628.
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    46. Racki LR, Narlikar GJ. ATP-dependent chromatin remodeling enzymes: two heads are not better, just different. Curr Opin Genet Dev. 2008 Apr; 18(2):137-44. PMID: 18339542.
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    47. Simon MD, Chu F, Racki LR, de la Cruz CC, Burlingame AL, Panning B, Narlikar GJ, Shokat KM. The site-specific installation of methyl-lysine analogs into recombinant histones. Cell. 2007 Mar 09; 128(5):1003-12. PMID: 17350582.
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    48. Yang JG, Narlikar GJ. FRET-based methods to study ATP-dependent changes in chromatin structure. Methods. 2007 Mar; 41(3):291-5. PMID: 17309839.
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    49. Yang JG, Madrid TS, Sevastopoulos E, Narlikar GJ. The chromatin-remodeling enzyme ACF is an ATP-dependent DNA length sensor that regulates nucleosome spacing. Nat Struct Mol Biol. 2006 Dec; 13(12):1078-83. PMID: 17099699.
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    50. He X, Fan HY, Narlikar GJ, Kingston RE. Human ACF1 alters the remodeling strategy of SNF2h. J Biol Chem. 2006 Sep 29; 281(39):28636-47. PMID: 16877760.
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    51. Mahajan MC, Narlikar GJ, Boyapaty G, Kingston RE, Weissman SM. Heterogeneous nuclear ribonucleoprotein C1/C2, MeCP1, and SWI/SNF form a chromatin remodeling complex at the beta-globin locus control region. Proc Natl Acad Sci U S A. 2005 Oct 18; 102(42):15012-7. PMID: 16217013.
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    52. Fan HY, Narlikar GJ, Kingston RE. Noncovalent modification of chromatin: different remodeled products with different ATPase domains. Cold Spring Harb Symp Quant Biol. 2004; 69:183-92. PMID: 16117648.
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    53. Fan HY, He X, Kingston RE, Narlikar GJ. Distinct strategies to make nucleosomal DNA accessible. Mol Cell. 2003 May; 11(5):1311-22. PMID: 12769854.
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    54. Aoyagi S, Narlikar G, Zheng C, Sif S, Kingston RE, Hayes JJ. Nucleosome remodeling by the human SWI/SNF complex requires transient global disruption of histone-DNA interactions. Mol Cell Biol. 2002 Jun; 22(11):3653-62. PMID: 11997502.
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    55. Narlikar GJ, Fan HY, Kingston RE. Cooperation between complexes that regulate chromatin structure and transcription. Cell. 2002 Feb 22; 108(4):475-87. PMID: 11909519.
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    56. Narlikar GJ, Phelan ML, Kingston RE. Generation and interconversion of multiple distinct nucleosomal states as a mechanism for catalyzing chromatin fluidity. Mol Cell. 2001 Dec; 8(6):1219-30. PMID: 11779498.
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    57. Aalfs JD, Narlikar GJ, Kingston RE. Functional differences between the human ATP-dependent nucleosome remodeling proteins BRG1 and SNF2H. J Biol Chem. 2001 Sep 07; 276(36):34270-8. PMID: 11435432.
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    58. Lee KM, Narlikar G. Assembly of nucleosomal templates by salt dialysis. Curr Protoc Mol Biol. 2001 May; Chapter 21:Unit 21.6. PMID: 18265198.
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    59. Jónsson ZO, Dhar SK, Narlikar GJ, Auty R, Wagle N, Pellman D, Pratt RE, Kingston R, Dutta A. Rvb1p and Rvb2p are essential components of a chromatin remodeling complex that regulates transcription of over 5% of yeast genes. J Biol Chem. 2001 May 11; 276(19):16279-88. PMID: 11278922.
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    60. Guyon JR, Narlikar GJ, Sullivan EK, Kingston RE. Stability of a human SWI-SNF remodeled nucleosomal array. Mol Cell Biol. 2001 Feb; 21(4):1132-44. PMID: 11158300.
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    61. Narlikar GJ, Bartley LE, Herschlag D. Use of duplex rigidity for stability and specificity in RNA tertiary structure. Biochemistry. 2000 May 23; 39(20):6183-9. PMID: 10821693.
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    62. Narlikar GJ, Bartley LE, Khosla M, Herschlag D. Characterization of a local folding event of the Tetrahymena group I ribozyme: effects of oligonucleotide substrate length, pH, and temperature on the two substrate binding steps. Biochemistry. 1999 Oct 26; 38(43):14192-204. PMID: 10571993.
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    63. Kingston RE, Narlikar GJ. ATP-dependent remodeling and acetylation as regulators of chromatin fluidity. Genes Dev. 1999 Sep 15; 13(18):2339-52. PMID: 10500090.
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    64. Shan SO, Narlikar GJ, Herschlag D. Protonated 2'-aminoguanosine as a probe of the electrostatic environment of the active site of the Tetrahymena group I ribozyme. Biochemistry. 1999 Aug 24; 38(34):10976-88. PMID: 10460152.
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    65. Guyon JR, Narlikar GJ, Sif S, Kingston RE. Stable remodeling of tailless nucleosomes by the human SWI-SNF complex. Mol Cell Biol. 1999 Mar; 19(3):2088-97. PMID: 10022896.
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    66. Phelan ML, Sif S, Narlikar GJ, Kingston RE. Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits. Mol Cell. 1999 Feb; 3(2):247-53. PMID: 10078207.
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    67. Narlikar GJ, Herschlag D. Direct demonstration of the catalytic role of binding interactions in an enzymatic reaction. Biochemistry. 1998 Jul 14; 37(28):9902-11. PMID: 9665695.
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    68. Narlikar GJ, Khosla M, Usman N, Herschlag D. Quantitating tertiary binding energies of 2' OH groups on the P1 duplex of the Tetrahymena ribozyme: intrinsic binding energy in an RNA enzyme. Biochemistry. 1997 Mar 04; 36(9):2465-77. PMID: 9054551.
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    69. Narlikar GJ, Herschlag D. Mechanistic aspects of enzymatic catalysis: lessons from comparison of RNA and protein enzymes. Annu Rev Biochem. 1997; 66:19-59. PMID: 9242901.
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    70. Narlikar GJ, Herschlag D. Isolation of a local tertiary folding transition in the context of a globally folded RNA. Nat Struct Biol. 1996 Aug; 3(8):701-10. PMID: 8756329.
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    71. Narlikar GJ, Gopalakrishnan V, McConnell TS, Usman N, Herschlag D. Use of binding energy by an RNA enzyme for catalysis by positioning and substrate destabilization. Proc Natl Acad Sci U S A. 1995 Apr 25; 92(9):3668-72. PMID: 7731962.
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    72. Knitt DS, Narlikar GJ, Herschlag D. Dissection of the role of the conserved G.U pair in group I RNA self-splicing. Biochemistry. 1994 Nov 22; 33(46):13864-79. PMID: 7947795.
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