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    Yifan Cheng, PhD

    TitleAssociate Professor
    SchoolUCSF School of Medicine
    DepartmentBiochemistry and Biophysics
    Address600 16th Street
    San Francisco CA 94158
    Phone415-514-9707

       Overview 
       Overview
      We are interested in studying the three-dimensional structures of macromolecular complexes: their structural architectures, the regulation of their function and the dynamic processes of their assembly and disassembly, by molecular electron microscopy (cryoEM). A full understanding of the biological functions/processes of any macromolecular complex requires structural information at a wide range of resolutions, including atomic details of its components, spatial arrangements of these components and interactions between them. CryoEM can be used to study macromolecules in their native conformations at a wide resolution range, from sub-nanometer resolution (single particle cryoEM) to the cellular level (electron tomography).


       ORNG Applications 
       Websites
       Awarded Grants
       More Info

       Bibliographic 
       Publications
      Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
      List All   |   Timeline
      1. Liao M, Cao E, Julius D, Cheng Y. Single particle electron cryo-microscopy of a mammalian ion channel. Curr Opin Struct Biol. 2014 Mar 25; 27C:1-7.
        View in: PubMed
      2. Liao M, Cao E, Julius D, Cheng Y. Structure of the TRPV1 ion channel determined by electron cryo-microscopy. Nature. 2013 Dec 5; 504(7478):107-12.
        View in: PubMed
      3. Li X, Zheng SQ, Egami K, Agard DA, Cheng Y. Influence of electron dose rate on electron counting images recorded with the K2 camera. J Struct Biol. 2013 Nov; 184(2):251-60.
        View in: PubMed
      4. Kim HM, Yu Y, Cheng Y. Structure characterization of the 26S proteasome. Biochim Biophys Acta. 2011 Feb; 1809(2):67-79.
        View in: PubMed
      5. Daugherty MD, Booth DS, Jayaraman B, Cheng Y, Frankel AD. HIV Rev response element (RRE) directs assembly of the Rev homooligomer into discrete asymmetric complexes. Proc Natl Acad Sci U S A. 2010 Jul 13; 107(28):12481-6.
        View in: PubMed
      6. Li X, Grigorieff N, Cheng Y. GPU-enabled FREALIGN: accelerating single particle 3D reconstruction and refinement in Fourier space on graphics processors. J Struct Biol. 2010 Dec; 172(3):407-12.
        View in: PubMed
      7. Lenassi M, Cagney G, Liao M, Vaupotic T, Bartholomeeusen K, Cheng Y, Krogan NJ, Plemenitas A, Peterlin BM. HIV Nef is secreted in exosomes and triggers apoptosis in bystander CD4+ T cells. Traffic. 2010 Jan; 11(1):110-22.
        View in: PubMed
      8. Racki LR, Yang JG, Naber N, Partensky PD, Acevedo A, Purcell TJ, Cooke R, Cheng Y, Narlikar GJ. The chromatin remodeller ACF acts as a dimeric motor to space nucleosomes. Nature. 2009 Dec 24; 462(7276):1016-21.
        View in: PubMed
      9. Yu Y, Smith DM, Kim HM, Rodriguez V, Goldberg AL, Cheng Y. Interactions of PAN's C-termini with archaeal 20S proteasome and implications for the eukaryotic proteasome-ATPase interactions. EMBO J. 2010 Feb 3; 29(3):692-702.
        View in: PubMed
      10. Li Z, Hite RK, Cheng Y, Walz T. Evaluation of imaging plates as recording medium for images of negatively stained single particles and electron diffraction patterns of two-dimensional crystals. J Electron Microsc (Tokyo). 2010; 59(1):53-63.
        View in: PubMed
      11. Cheng Y. Toward an atomic model of the 26S proteasome. Curr Opin Struct Biol. 2009 Apr; 19(2):203-8.
        View in: PubMed
      12. Kim HZ, Jung K, Kim HM, Cheng Y, Koh GY. A designed angiopoietin-2 variant, pentameric COMP-Ang2, strongly activates Tie2 receptor and stimulates angiogenesis. Biochim Biophys Acta. 2009 May; 1793(5):772-80.
        View in: PubMed
      13. Cheng Y, Walz T. The advent of near-atomic resolution in single-particle electron microscopy. Annu Rev Biochem. 2009; 78:723-42.
        View in: PubMed
      14. Rabl J, Smith DM, Yu Y, Chang SC, Goldberg AL, Cheng Y. Mechanism of gate opening in the 20S proteasome by the proteasomal ATPases. Mol Cell. 2008 May 9; 30(3):360-8.
        View in: PubMed
      15. Frey G, Peng H, Rits-Volloch S, Morelli M, Cheng Y, Chen B. A fusion-intermediate state of HIV-1 gp41 targeted by broadly neutralizing antibodies. Proc Natl Acad Sci U S A. 2008 Mar 11; 105(10):3739-44.
        View in: PubMed
      16. Ballister ER, Lai AH, Zuckermann RN, Cheng Y, Mougous JD. In vitro self-assembly of tailorable nanotubes from a simple protein building block. Proc Natl Acad Sci U S A. 2008 Mar 11; 105(10):3733-8.
        View in: PubMed
      17. Ohi MD, Feoktistova A, Ren L, Yip C, Cheng Y, Chen JS, Yoon HJ, Wall JS, Huang Z, Penczek PA, Gould KL, Walz T. Structural organization of the anaphase-promoting complex bound to the mitotic activator Slp1. Mol Cell. 2007 Dec 14; 28(5):871-85.
        View in: PubMed
      18. Gonen T, Hite RK, Cheng Y, Petre BM, Kistler J, Walz T. Polymorphic assemblies and crystalline arrays of lens tetraspanin MP20. J Mol Biol. 2008 Feb 15; 376(2):380-92.
        View in: PubMed
      19. Smith DM, Chang SC, Park S, Finley D, Cheng Y, Goldberg AL. Docking of the proteasomal ATPases' carboxyl termini in the 20S proteasome's alpha ring opens the gate for substrate entry. Mol Cell. 2007 Sep 7; 27(5):731-44.
        View in: PubMed
      20. Cheng Y, Walz T. Reconstructing the endocytotic machinery. Methods Cell Biol. 2007; 79:463-87.
        View in: PubMed
      21. Cheng Y, Boll W, Kirchhausen T, Harrison SC, Walz T. Cryo-electron tomography of clathrin-coated vesicles: structural implications for coat assembly. J Mol Biol. 2007 Jan 19; 365(3):892-9.
        View in: PubMed
      22. Nishida N, Xie C, Shimaoka M, Cheng Y, Walz T, Springer TA. Activation of leukocyte beta2 integrins by conversion from bent to extended conformations. Immunity. 2006 Oct; 25(4):583-94.
        View in: PubMed
      23. Liu J, Wu S, Reedy MC, Winkler H, Lucaveche C, Cheng Y, Reedy MK, Taylor KA. Electron tomography of swollen rigor fibers of insect flight muscle reveals a short and variably angled S2 domain. J Mol Biol. 2006 Sep 29; 362(4):844-60.
        View in: PubMed
      24. Fotin A, Kirchhausen T, Grigorieff N, Harrison SC, Walz T, Cheng Y. Structure determination of clathrin coats to subnanometer resolution by single particle cryo-electron microscopy. J Struct Biol. 2006 Dec; 156(3):453-60.
        View in: PubMed
      25. Nakagawa T, Cheng Y, Sheng M, Walz T. Three-dimensional structure of an AMPA receptor without associated stargazin/TARP proteins. Biol Chem. 2006 Feb; 387(2):179-87.
        View in: PubMed
      26. Smith DM, Kafri G, Cheng Y, Ng D, Walz T, Goldberg AL. ATP binding to PAN or the 26S ATPases causes association with the 20S proteasome, gate opening, and translocation of unfolded proteins. Mol Cell. 2005 Dec 9; 20(5):687-98.
        View in: PubMed
      27. Gonen T, Cheng Y, Sliz P, Hiroaki Y, Fujiyoshi Y, Harrison SC, Walz T. Lipid-protein interactions in double-layered two-dimensional AQP0 crystals. Nature. 2005 Dec 1; 438(7068):633-8.
        View in: PubMed
      28. Cheng Y, Zak O, Aisen P, Harrison SC, Walz T. Single particle reconstruction of the human apo-transferrin-transferrin receptor complex. J Struct Biol. 2005 Dec; 152(3):204-10.
        View in: PubMed
      29. Cheng Y, Wolf E, Larvie M, Zak O, Aisen P, Grigorieff N, Harrison SC, Walz T. Single particle reconstructions of the transferrin-transferrin receptor complex obtained with different specimen preparation techniques. J Mol Biol. 2006 Feb 3; 355(5):1048-65.
        View in: PubMed
      30. Nakagawa T, Cheng Y, Ramm E, Sheng M, Walz T. Structure and different conformational states of native AMPA receptor complexes. Nature. 2005 Feb 3; 433(7025):545-9.
        View in: PubMed
      31. Fotin A, Cheng Y, Sliz P, Grigorieff N, Harrison SC, Kirchhausen T, Walz T. Molecular model for a complete clathrin lattice from electron cryomicroscopy. Nature. 2004 Dec 2; 432(7017):573-9.
        View in: PubMed
      32. Fotin A, Cheng Y, Grigorieff N, Walz T, Harrison SC, Kirchhausen T. Structure of an auxilin-bound clathrin coat and its implications for the mechanism of uncoating. Nature. 2004 Dec 2; 432(7017):649-53.
        View in: PubMed
      33. Gonen T, Cheng Y, Kistler J, Walz T. Aquaporin-0 membrane junctions form upon proteolytic cleavage. J Mol Biol. 2004 Sep 24; 342(4):1337-45.
        View in: PubMed
      34. Gonen T, Sliz P, Kistler J, Cheng Y, Walz T. Aquaporin-0 membrane junctions reveal the structure of a closed water pore. Nature. 2004 May 13; 429(6988):193-7.
        View in: PubMed
      35. Chen B, Cheng Y, Calder L, Harrison SC, Reinherz EL, Skehel JJ, Wiley DC. A chimeric protein of simian immunodeficiency virus envelope glycoprotein gp140 and Escherichia coli aspartate transcarbamoylase. J Virol. 2004 May; 78(9):4508-16.
        View in: PubMed
      36. Ohi M, Li Y, Cheng Y, Walz T. Negative Staining and Image Classification - Powerful Tools in Modern Electron Microscopy. Biol Proced Online. 2004; 6:23-34.
        View in: PubMed
      37. Cheng Y, Zak O, Aisen P, Harrison SC, Walz T. Structure of the human transferrin receptor-transferrin complex. Cell. 2004 Feb 20; 116(4):565-76.
        View in: PubMed
      38. Toth EA, Li Y, Sawaya MR, Cheng Y, Ellenberger T. The crystal structure of the bifunctional primase-helicase of bacteriophage T7. Mol Cell. 2003 Nov; 12(5):1113-23.
        View in: PubMed
      39. Lederkremer GZ, Cheng Y, Petre BM, Vogan E, Springer S, Schekman R, Walz T, Kirchhausen T. Structure of the Sec23p/24p and Sec13p/31p complexes of COPII. Proc Natl Acad Sci U S A. 2001 Sep 11; 98(19):10704-9.
        View in: PubMed
      40. Schmidt-Krey I, Mitsuoka K, Hirai T, Murata K, Cheng Y, Fujiyoshi Y, Morgenstern R, Hebert H. The three-dimensional map of microsomal glutathione transferase 1 at 6 A resolution. EMBO J. 2000 Dec 1; 19(23):6311-6.
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      41. Schmidt-Krey I, Murata K, Hirai T, Mitsuoka K, Cheng Y, Morgenstern R, Fujiyoshi Y, Hebert H. The projection structure of the membrane protein microsomal glutathione transferase at 3 A resolution as determined from two-dimensional hexagonal crystals. J Mol Biol. 1999 Apr 30; 288(2):243-53.
        View in: PubMed
      42. Hiroaki Y, Mitsuoka K, Cheng Y, Hiroaki H, Fujiyoshi Y. Influence of various nucleotides on the in situ crystallization of Ca2+-ATPase. Biochim Biophys Acta. 1999 Jan 8; 1415(2):361-8.
        View in: PubMed
      43. Cheng Y, Taylor KA. Characterization of the low magnification performance of a Philips CM300-FEG. Ultramicroscopy. 1998 Sep; 74(4):209-20.
        View in: PubMed
      44. Taylor KA, Tang J, Cheng Y, Winkler H. The use of electron tomography for structural analysis of disordered protein arrays. J Struct Biol. 1997 Dec; 120(3):372-86.
        View in: PubMed
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