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Nadia Roan, PhD

Title(s)Associate Professor, Urology
SchoolSchool of Medicine
Phone415-734-4883
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    Collapse Biography 
    Collapse Awards and Honors
    Program for Breakthrough Biomedical Research2020  - 2021PBBR COVID-19 Grant
    NIH2019  - 2023R01AI147777. PI: Roan
    NIH2017  - 2022P01AI131374. PI of P01: Greene, PI of Project 1: Roan
    NIH2016  - 2021R01AI127219. PI: Roan
    NIH2016  - 2018R21AI122821. PI: Roan
    NIH2015  - 2017R21AI116252. PI: Roan
    NIH2018  - 2023R01AI141003. PI: Henrich
    NIH2017  - 2020R15GM123447. PI: Jensen-Seaman
    amfAR2016  - 2021amfAR Cure Institute
    UCSF, NIH2016  - 2017NCTRI P50 Pilot Grant Award. PI: Roan
    NIH2016  - 2017WIHS W16036 PI: Yukl
    CFAR2014  - 2015Center for AIDS Research Basic Science HIV/AIDS grant
    NIH2013  - 2016K99/R00 (K99AI103262, R00AI104262). PI: Roan
    UCSF2013  - 2015Hellman Award for Early-Career Faculty
    NIH2012  - 2013KURe K12
    NIH2012  - 2017R01HD074511. PI: Pilcher
    CFAR2012Centers for AIDS Research Early Career Award of Excellence in Basic Research
    The J. David Gladstones Institutes2012Gladstone Institute of Virology and Immunology Award for Scientific Excellence
    A.P. Giannini Foundation2008  - 2010A.P. Giannini Foundation Postdoctoral Fellowship
    NIH2006Travel Award for the 11th International Symposium on Human Chlamydial Infections
    Harvard Medical School2004Manfred Karnovsky Fellowship Award
    U.C. Berkeley2001Outstanding Scholar Award
    NSF2001  - 2004National Science Foundation Pre-Doctoral Fellowship
    U.C. Berkeley2001F.H. Carpenter Memorial Price in Biochemistry
    U.C. Berkeley2001University Medal Finalist

    Collapse Overview 
    Collapse Overview
    The Roan Lab studies how intracellular and extracellular factors in the tissue microenvironment can affect infection by HIV, mucosal immunity, and reproductive health. We have demonstrated that genital and rectal fibroblasts, amongst the most abundant cells of the mucosa, potently increase HIV infection of T cells through at least two distinct mechanisms: promoting viral entry, and altering the cellular state of T cells to render them more permissive to viral replication. To characterize the molecular basis of how intrinsic and extrinsic perturbations can render some subsets of CD4+ T cells more susceptible than others to HIV infection, we are using a variety of global gene expression analysis approaches, including CyTOF and RNA-seq. These approaches are also being used to characterize the HIV latent reservoir and the nature of viral rebound upon antiretroviral treatment interruption. Another research interest in the lab is to understand how factors in seminal plasma affect reproductive health and susceptibility to sexually transmitted diseases.

    Collapse Research 
    Collapse Research Activities and Funding
    Phenotypic and mechanistic analysis of the in vivo HIV latent reservoir by single-cell technologies
    NIH/NIAID R01AI147777Aug 1, 2019 - Jul 31, 2023
    Role: Principal Investigator
    Targeting Non Viral Markers of HIV Persistence
    NIH/NIAID R01AI141003May 15, 2018 - Apr 30, 2023
    Role: Co-Investigator
    Exploiting the Host-HIV Interface To Identify Biomarkers Predicting Time to Viral Rebound after Treatment Interruption
    NIH/NIAID P01AI131374Aug 8, 2017 - Jul 31, 2022
    Role: Principal Investigator of Project 1
    Elucidating the mechanism of progesterone-induced permissivity in the upper female reproductive tract
    NIH/NIAID R01AI127219Sep 26, 2016 - Aug 31, 2021
    Role: Principal Investigator
    Characterization of Exosomes From Semen of Uninfected and HIV-Infected Men
    NIH/NIAID R21AI122821Mar 1, 2016 - Feb 28, 2018
    Role: Principal Investigator
    Characterization of Gallic Acid as a Novel HIV Microbicide Candidate
    NIH/NIAID R21AI116252Jul 1, 2015 - Jun 30, 2017
    Role: Principal Investigator
    Exploring the Role of Semen Amyloids in Promoting HIV Infection and Fertilization
    NIH/NIAID R00AI104262May 15, 2013 - Nov 30, 2016
    Role: Principal Investigator
    Exploring the Role of Semen Amyloids in Promoting HIV Infection and Fertilization
    NIH/NIAID K99AI104262May 15, 2013 - Apr 30, 2014
    Role: Principal Investigator

    Collapse ORNG Applications 
    Collapse Featured Publications
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    Collapse Featured Presentations

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
    List All   |   Timeline
    1. Ma T, Luo X, George AF, Mukherjee G, Sen N, Spitzer TL, Giudice LC, Greene WC, Roan NR. HIV efficiently infects T cells from the endometrium and remodels them to promote systemic viral spread. Elife. 2020 May 26; 9. PMID: 32452381.
      View in: PubMed
    2. Marques de Menezes EG, Jang K, George AF, Nyegaard M, Neidleman J, Inglis HC, Danesh A, Deng X, Afshari A, Kim YH, Billaud JN, Marson K, Pilcher CD, Pillai SK, Norris PJ, Roan NR. Seminal plasma-derived extracellular vesicle fractions from HIV-infected men exhibit unique miRNA signatures and induce a pro-inflammatory response in cells isolated from the female reproductive tract. J Virol. 2020 May 20. PMID: 32434889.
      View in: PubMed
    3. Hsiao F, Frouard J, Gramatica A, Xie G, Telwatte S, Lee GQ, Roychoudhury P, Schwarzer R, Luo X, Yukl SA, Lee S, Hoh R, Deeks SG, Jones RB, Cavrois M, Greene WC, Roan NR. Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection. PLoS Pathog. 2020 Apr; 16(4):e1008450. PMID: 32353080.
      View in: PubMed
    4. George AF, Jang KS, Nyegaard M, Neidleman J, Spitzer TL, Xie G, Chen JC, Herzig E, Laustsen A, Marques de Menezes EG, Houshdaran S, Pilcher CD, Norris PJ, Jakobsen MR, Greene WC, Giudice LC, Roan NR. Seminal plasma promotes decidualization of endometrial stromal fibroblasts in vitro from women with and without inflammatory disorders in a manner dependent on interleukin-11 signaling. Hum Reprod. 2020 Mar 27; 35(3):617-640. PMID: 32219408.
      View in: PubMed
    5. James KS, Trumble I, Clohosey ML, Moeser M, Roan NR, Adimora AA, Joseph SB, Archin NM, Hudgens M, Soriano-Sarabia N. Measuring the contribution of ?d T cells to the persistent HIV reservoir. AIDS. 2020 03 01; 34(3):363-371. PMID: 31764074.
      View in: PubMed
    6. Thomas A, Hammarlund E, Gao L, Holman S, Michel KG, Glesby M, Villacres MC, Golub ET, Roan NR, French AL, Augenbraun MH, Slifka MK. Loss of Pre-Existing Immunological Memory among HIV Infected Women Despite Immune Reconstitution with Antiretroviral Therapy. J Infect Dis. 2019 Dec 23. PMID: 31867597.
      View in: PubMed
    7. Cavrois M, Hilton JF, Roan NR, Takeda M, Seidman D, Averbach S, Chang E, Raman N, Greenblatt R, Shacklett BL, Smith-McCune K. Effects of the levonorgestrel-containing intrauterine device, copper intrauterine device, and levonorgestrel-containing oral contraceptive on susceptibility of immune cells from cervix, endometrium and blood to HIV-1 fusion measured ex vivo. PLoS One. 2019; 14(8):e0221181. PMID: 31437197.
      View in: PubMed
    8. Skinner WM, Mannowetz N, Lishko PV, Roan NR. Single-cell Motility Analysis of Tethered Human Spermatozoa. Bio Protoc. 2019 Mar 05; 9(5). PMID: 31032381.
      View in: PubMed
    9. Laustsen A, Bak RO, Krapp C, Kjær L, Egedahl JH, Petersen CC, Pillai S, Tang HQ, Uldbjerg N, Porteus M, Roan NR, Nyegaard M, Denton PW, Jakobsen MR. Interferon priming is essential for human CD34+ cell-derived plasmacytoid dendritic cell maturation and function. Nat Commun. 2018 08 30; 9(1):3525. PMID: 30166549.
      View in: PubMed
    10. Martin JW, Chen JC, Neidleman J, Tatsumi K, Hu J, Giudice LC, Greene WC, Roan NR. Potent and rapid activation of tropomyosin-receptor kinase A in endometrial stromal fibroblasts by seminal plasma. Biol Reprod. 2018 08 01; 99(2):336-348. PMID: 29518187.
      View in: PubMed
    11. Röcker A, Roan NR, Yadav JK, Fändrich M, Münch J. Structure, function and antagonism of semen amyloids. Chem Commun (Camb). 2018 Jul 05; 54(55):7557-7569. PMID: 29873340.
      View in: PubMed
    12. Müller JA, Harms M, Krüger F, Groß R, Joas S, Hayn M, Dietz AN, Lippold S, von Einem J, Schubert A, Michel M, Mayer B, Cortese M, Jang KS, Sandi-Monroy N, Deniz M, Ebner F, Vapalahti O, Otto M, Bartenschlager R, Herbeuval JP, Schmidt-Chanasit J, Roan NR, Münch J. Semen inhibits Zika virus infection of cells and tissues from the anogenital region. Nat Commun. 2018 06 07; 9(1):2207. PMID: 29880824.
      View in: PubMed
    13. Roan NR. Resident T cells stand up to HIV. Sci Immunol. 2018 06 01; 3(24). PMID: 29858287.
      View in: PubMed
    14. Trapecar M, Khan S, Roan NR, Chen TH, Telwatte S, Deswal M, Pao M, Somsouk M, Deeks SG, Hunt PW, Yukl S, Sanjabi S. An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies. AIDS Res Hum Retroviruses. 2017 Nov; 33(S1):S31-S39. PMID: 28882052.
      View in: PubMed
    15. Cavrois M, Banerjee T, Mukherjee G, Raman N, Hussien R, Rodriguez BA, Vasquez J, Spitzer MH, Lazarus NH, Jones JJ, Ochsenbauer C, McCune JM, Butcher EC, Arvin AM, Sen N, Greene WC, Roan NR. Mass Cytometric Analysis of HIV Entry, Replication, and Remodeling in Tissue CD4+ T Cells. Cell Rep. 2017 07 25; 20(4):984-998. PMID: 28746881.
      View in: PubMed
    16. Roan NR, Sandi-Monroy N, Kohgadai N, Usmani SM, Hamil KG, Neidleman J, Montano M, Ständker L, Röcker A, Cavrois M, Rosen J, Marson K, Smith JF, Pilcher CD, Gagsteiger F, Sakk O, O'Rand M, Lishko PV, Kirchhoff F, Münch J, Greene WC. Semen amyloids participate in spermatozoa selection and clearance. Elife. 2017 06 27; 6. PMID: 28653619.
      View in: PubMed
    17. Neidleman JA, Chen JC, Kohgadai N, Müller JA, Laustsen A, Thavachelvam K, Jang KS, Stürzel CM, Jones JJ, Ochsenbauer C, Chitre A, Somsouk M, Garcia MM, Smith JF, Greenblatt RM, Münch J, Jakobsen MR, Giudice LC, Greene WC, Roan NR. Mucosal stromal fibroblasts markedly enhance HIV infection of CD4+ T cells. PLoS Pathog. 2017 02; 13(2):e1006163. PMID: 28207890.
      View in: PubMed
    18. Camus C, Matusali G, Bourry O, Mahe D, Aubry F, Bujan L, Pasquier C, Massip P, Ravel C, Zirafi O, Munch J, Roan NR, Pineau C, Dejucq-Rainsford N. Comparison of the effect of semen from HIV-infected and uninfected men on CD4+ T-cell infection. AIDS. 2016 05 15; 30(8):1197-208. PMID: 26854806.
      View in: PubMed
    19. LoRicco JG, Xu CS, Neidleman J, Bergkvist M, Greene WC, Roan NR, Makhatadze GI. Gallic Acid Is an Antagonist of Semen Amyloid Fibrils That Enhance HIV-1 Infection. J Biol Chem. 2016 Jul 01; 291(27):14045-55. PMID: 27226574.
      View in: PubMed
    20. Bergman P, Roan NR, Römling U, Bevins CL, Münch J. Amyloid formation: functional friend or fearful foe? J Intern Med. 2016 Aug; 280(2):139-52. PMID: 27151743.
      View in: PubMed
    21. Müller JA, Zirafi O, Roan NR, Lee SJ, Münch J. Evaluation of EPI-X4 as a urinary peptide biomarker for diagnosis and prognosis of late acute GvHD. Bone Marrow Transplant. 2016 08; 51(8):1137-9. PMID: 27042833.
      View in: PubMed
    22. Roan NR, Jakobsen MR. Friend or Foe: Innate Sensing of HIV in the Female Reproductive Tract. Curr HIV/AIDS Rep. 2016 Feb; 13(1):53-63. PMID: 26879654.
      View in: PubMed
    23. Chen JC, Roan NR. Isolation and Culture of Human Endometrial Epithelial Cells and Stromal Fibroblasts. Bio Protoc. 2015 Oct 20; 5(20). PMID: 27347495.
      View in: PubMed
    24. Roan NR, Münch J. Improving preclinical models of HIV microbicide efficacy. Trends Microbiol. 2015 Aug; 23(8):445-7. PMID: 26250616.
      View in: PubMed
    25. Zirafi O, Kim KA, Roan NR, Kluge SF, Müller JA, Jiang S, Mayer B, Greene WC, Kirchhoff F, Münch J. Semen enhances HIV infectivity and impairs the antiviral efficacy of microbicides. Sci Transl Med. 2014 Nov 12; 6(262):262ra157. PMID: 25391483.
      View in: PubMed
    26. Onofrio Zirafi, Kyeong-Ae Kim, Nadia R. Roan, Benjamin Mayer, Silvia Kluge, Shibo Jiang, Warner C. Greene, Frank Kirchhoff, Jan Münch. Semen-mediated Enhancement of HIV Infection Markedly Impairs the Antiviral Efficacy of Microbicides. AIDS Research and Human Retroviruses. 2014 Oct 1; 30(S1):a183-a183.
      View in: Publisher Site
    27. Shariq Usmani, Haichuan Liu, Chris D. Pilcher, H. Ewa Witkowska, Frank Kirchhoff, Warner C. Greene, Jan Münch, Nadia R. Roan. HIV-enhancing Amyloids Are Prevalent in Fresh Semen and Are a Determinant for Semen's Ability to Enhance HIV Infection: Relevance for HIV Transmission. AIDS Research and Human Retroviruses. 2014 Oct 1; 30(S1):a183-a184.
      View in: Publisher Site
    28. French KC, Roan NR, Makhatadze GI. Structural characterization of semen coagulum-derived SEM1(86-107) amyloid fibrils that enhance HIV-1 infection. Biochemistry. 2014 May 27; 53(20):3267-77. PMID: 24811874.
      View in: PubMed
    29. Roan NR, Liu H, Usmani SM, Neidleman J, Müller JA, Avila-Herrera A, Gawanbacht A, Zirafi O, Chu S, Dong M, Kumar ST, Smith JF, Pollard KS, Fändrich M, Kirchhoff F, Münch J, Witkowska HE, Greene WC. Liquefaction of semen generates and later degrades a conserved semenogelin peptide that enhances HIV infection. J Virol. 2014 Jul; 88(13):7221-34. PMID: 24741080.
      View in: PubMed
    30. Roan NR, Chu S, Liu H, Neidleman J, Witkowska HE, Greene WC. Interaction of fibronectin with semen amyloids synergistically enhances HIV infection. J Infect Dis. 2014 Oct 01; 210(7):1062-6. PMID: 24719472.
      View in: PubMed
    31. Usmani SM, Zirafi O, Müller JA, Sandi-Monroy NL, Yadav JK, Meier C, Weil T, Roan NR, Greene WC, Walther P, Nilsson KP, Hammarström P, Wetzel R, Pilcher CD, Gagsteiger F, Fändrich M, Kirchhoff F, Münch J. Direct visualization of HIV-enhancing endogenous amyloid fibrils in human semen. Nat Commun. 2014 Apr 01; 5:3508. PMID: 24691351.
      View in: PubMed
    32. Chen JC, Johnson BA, Erikson DW, Piltonen TT, Barragan F, Chu S, Kohgadai N, Irwin JC, Greene WC, Giudice LC, Roan NR. Seminal plasma induces global transcriptomic changes associated with cell migration, proliferation and viability in endometrial epithelial cells and stromal fibroblasts. Hum Reprod. 2014 Jun; 29(6):1255-70. PMID: 24626806.
      View in: PubMed
    33. Tang Q, Roan NR, Yamamura Y. Seminal plasma and semen amyloids enhance cytomegalovirus infection in cell culture. J Virol. 2013 Dec; 87(23):12583-91. PMID: 24027327.
      View in: PubMed
    34. Yolamanova M, Meier C, Shaytan AK, Vas V, Bertoncini CW, Arnold F, Zirafi O, Usmani SM, Müller JA, Sauter D, Goffinet C, Palesch D, Walther P, Roan NR, Geiger H, Lunov O, Simmet T, Bohne J, Schrezenmeier H, Schwarz K, Ständker L, Forssmann WG, Salvatella X, Khalatur PG, Khokhlov AR, Knowles TP, Weil T, Kirchhoff F, Münch J. Peptide nanofibrils boost retroviral gene transfer and provide a rapid means for concentrating viruses. Nat Nanotechnol. 2013 Feb; 8(2):130-6. PMID: 23334171.
      View in: PubMed
    35. B.A. Johnson, J. Chen, N. Roan, F. Barragan, J. Irwin, L.C. Giudice. Pro-inflammatory effects of seminal plasma on human endometrium: implications for implantation and human immunodeficiency virus (HIV) transmission. Fertility and Sterility. 2012 Sep 1; 98(3):s221.
      View in: Publisher Site
    36. Roan NR, Cavrois M, Greene WC. [Role of semen-derived amyloid fibrils as facilitators of HIV infection]. Med Sci (Paris). 2012 Apr; 28(4):358-60. PMID: 22549858.
      View in: PubMed
    37. Roan NR, Müller JA, Liu H, Chu S, Arnold F, Stürzel CM, Walther P, Dong M, Witkowska HE, Kirchhoff F, Münch J, Greene WC. Peptides released by physiological cleavage of semen coagulum proteins form amyloids that enhance HIV infection. Cell Host Microbe. 2011 Dec 15; 10(6):541-50. PMID: 22177559.
      View in: PubMed
    38. Arnold F, Schnell J, Zirafi O, Stürzel C, Meier C, Weil T, Ständker L, Forssmann WG, Roan NR, Greene WC, Kirchhoff F, Münch J. Naturally occurring fragments from two distinct regions of the prostatic acid phosphatase form amyloidogenic enhancers of HIV infection. J Virol. 2012 Jan; 86(2):1244-9. PMID: 22090109.
      View in: PubMed
    39. Kim KA, Yolamanova M, Zirafi O, Roan NR, Staendker L, Forssmann WG, Burgener A, Dejucq-Rainsford N, Hahn BH, Shaw GM, Greene WC, Kirchhoff F, Münch J. Semen-mediated enhancement of HIV infection is donor-dependent and correlates with the levels of SEVI. Retrovirology. 2010 Jun 23; 7:55. PMID: 20573198.
      View in: PubMed
    40. Roan NR, Sowinski S, Münch J, Kirchhoff F, Greene WC. Aminoquinoline surfen inhibits the action of SEVI (semen-derived enhancer of viral infection). J Biol Chem. 2010 Jan 15; 285(3):1861-9. PMID: 19897482.
      View in: PubMed
    41. Gondek DC, Roan NR, Starnbach MN. T cell responses in the absence of IFN-gamma exacerbate uterine infection with Chlamydia trachomatis. J Immunol. 2009 Jul 15; 183(2):1313-9. PMID: 19561106.
      View in: PubMed
    42. Roan NR, Münch J, Arhel N, Mothes W, Neidleman J, Kobayashi A, Smith-McCune K, Kirchhoff F, Greene WC. The cationic properties of SEVI underlie its ability to enhance human immunodeficiency virus infection. J Virol. 2009 Jan; 83(1):73-80. PMID: 18945786.
      View in: PubMed
    43. Starnbach MN, Roan NR. Conquering sexually transmitted diseases. Nat Rev Immunol. 2008 Apr; 8(4):313-7. PMID: 18309315.
      View in: PubMed
    44. Grotenbreg GM, Roan NR, Guillen E, Meijers R, Wang JH, Bell GW, Starnbach MN, Ploegh HL. Discovery of CD8+ T cell epitopes in Chlamydia trachomatis infection through use of caged class I MHC tetramers. Proc Natl Acad Sci U S A. 2008 Mar 11; 105(10):3831-6. PMID: 18245382.
      View in: PubMed
    45. Roan NR, Greene WC. A seminal finding for understanding HIV transmission. Cell. 2007 Dec 14; 131(6):1044-6. PMID: 18083095.
      View in: PubMed
    46. Roan NR, Starnbach MN. Immune-mediated control of Chlamydia infection. Cell Microbiol. 2008 Jan; 10(1):9-19. PMID: 17979983.
      View in: PubMed
    47. Nadia R. Roan, Michael N. Starnbach. Immune-mediated control of Chlamydia infection. Cellular Microbiology. 2007 Nov 2; 0(0):071103031556005-???.
      View in: Publisher Site
    48. Roan NR, Starnbach MN. Antigen-specific CD8+ T cells respond to Chlamydia trachomatis in the genital mucosa. J Immunol. 2006 Dec 01; 177(11):7974-9. PMID: 17114470.
      View in: PubMed
    49. Roan NR, Gierahn TM, Higgins DE, Starnbach MN. Monitoring the T cell response to genital tract infection. Proc Natl Acad Sci U S A. 2006 Aug 08; 103(32):12069-74. PMID: 16880389.
      View in: PubMed
    50. Balsara ZR, Roan NR, Steele LN, Starnbach MN. Developmental regulation of Chlamydia trachomatis class I accessible protein-1, a CD8+ T cell antigen. J Infect Dis. 2006 May 15; 193(10):1459-63. PMID: 16619195.
      View in: PubMed
    51. Hess D, Liu B, Roan NR, Sternglanz R, Winston F. Spt10-dependent transcriptional activation in Saccharomyces cerevisiae requires both the Spt10 acetyltransferase domain and Spt21. Mol Cell Biol. 2004 Jan; 24(1):135-43. PMID: 14673149.
      View in: PubMed
    52. D'Orazio SE, Velasquez M, Roan NR, Naveiras-Torres O, Starnbach MN. The Listeria monocytogenes lemA gene product is not required for intracellular infection or to activate fMIGWII-specific T cells. Infect Immun. 2003 Dec; 71(12):6721-7. PMID: 14638756.
      View in: PubMed
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