David Nguyen PhD MD is a physician-scientist in Infectious Diseases and studies applications of genome editing in the immune system as applied to understanding and correcting mutations causing primary immunodeficiency (PID). He completed his PhD in Materials Science and Medical Engineering at MIT in the HST program, where he studied nanoparticle delivery of DNA and RNA to the immune system in the laboratory of Prof. Robert Langer with applications in DNA vaccines and immunostimulatory RNA vaccine adjuvants. He then went to Stanford University for medical school, and since 2012 has been at the UCSF Medical Center where he completed medical residency and a clinical fellowship in Infectious Diseases. He is currently an Assistant Adjunct Professor in the Department of Medicine at UCSF, and is a research fellow of the UCSF CIRM Alpha Stem Cell Clinic working in the laboratory of Prof. Alex Marson. Dr. Nguyen has clinical interests in treating infections in patients with compromised immune systems, and research interests in therapeutic gene editing to correct mutations that drive PID. Dr. Nguyen has leveraged his background in nucleic acid delivery to create new tools for high-efficiency non-viral CRISPR Cas9 gene editing in primary human immune cells. He is applying these tools to discover and model mutations associated with PIDs with an eventual goal of developing novel non-viral gene therapy strategies in human HSCs and T cells for treatment of patients living with a PID disease.