Jason Gestwicki, PhD

InstitutionUniversity of California San Francisco
DepartmentInstitute for Neurodegenerative Diseases
Address675 Nelson Rising Lane
San Francisco CA 94158
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    Collapse Biography 
    Collapse Awards and Honors
    University of Michigan2012American Association for the Advancement of Science (AAAS) Fellow
    University of Michigan2009NSF CAREER Award
    University of Michigan2006McKnight Foundation's Brain Disorders Award
    University of Michigan2005Biological Sciences Scholar
    Stanford Univeristy2003Helen Hay Whitney Foundation Fellow
    University of Wisconsin - Madison2001Sigrid Leirmo Award
    University of Wisconsin - Madison1998NIH Biotechnology Training Fellow

    Collapse Overview 
    Collapse Overview
    The Gestwicki Laboratory is interested in molecular chaperones, protein homeostasis and protein misfolding disorders. To approach the big questions in this area, we use a chemical biology strategy that includes the discovery and optimization of new chemical inhibitors. We use these chemical probes to acutely perturb chaperone functions, revealing how these systems normally protect from cancer and neurodegeneration. Many of our chemical probes target allosteric and protein-protein interaction sites on the molecular chaperones and we have a significant interest in discovering inhibitors of "difficult" targets. We hope to better understand the logic of protein folding and misfolding and use this information to design more effective therapies.

    Collapse Research 
    Collapse Research Activities and Funding
    Activation of the 20S Proteasome to Normalize Tau Homeostasis
    NIH/NIA R21AG053619Aug 15, 2016 - May 31, 2018
    Role: Principal Investigator
    Exploration of Molecular Chaperone Complexes During Active Protein Triage
    NIH/NIGMS R01GM109896Jun 1, 2014 - Feb 28, 2018
    Role: Co-Principal Investigator
    Chemical Probes and Chaperone-Accelerated Turnover of Tau
    NIH/NIA R21AG042813Aug 1, 2012 - Jul 31, 2014
    Role: Principal Investigator
    Natural Product-Inspired Method for Enhancing HIV Protease Inhibitors
    NIH/NIAID R21AI098431Feb 1, 2012 - Jan 31, 2014
    Role: Principal Investigator
    Molecular Chaperones and Small Molecules
    NIH/NINDS R01NS059690Feb 1, 2008 - Jul 31, 2018
    Role: Principal Investigator

    Collapse ORNG Applications 
    Collapse Global Health
    Collapse Websites

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. Shao H, Li X, Moses MA, Gilbert LA, Kalyanaraman C, Young ZT, Chernova M, Journey SN, Weissman JS, Hann B, Jacobson MP, Neckers LM, Gestwicki J. Exploration of Benzothiazole-Rhodacyanines as Allosteric Inhibitors of Protein-Protein Interactions with Heat Shock Protein 70 (Hsp70). J Med Chem. 2018 Jun 28. PMID: 29953808.
      View in: PubMed
    2. Ran X, Gestwicki J. Inhibitors of protein-protein interactions (PPIs): an analysis of scaffold choices and buried surface area. Curr Opin Chem Biol. 2018 Jun 13; 44:75-86. PMID: 29908451.
      View in: PubMed
    3. Glazier AA, Hafeez N, Mellacheruvu D, Basrur V, Nesvizhskii AI, Lee LM, Shao H, Tang V, Yob JM, Gestwicki J, Helms AS, Day SM. HSC70 is a chaperone for wild-type and mutant cardiac myosin binding protein C. JCI Insight. 2018 Jun 07; 3(11). PMID: 29875314.
      View in: PubMed
    4. Schwarz DMC, Gestwicki J. Revisiting the "Art of the Chemical Probe". ACS Chem Biol. 2018 May 18; 13(5):1109-1110. PMID: 29772907.
      View in: PubMed
    5. Moses MA, Kim YS, Rivera-Marquez GM, Oshima N, Watson MJ, Beebe K, Wells C, Lee S, Zuehlke AD, Shao H, Bingman WE, Kumar V, Malhotra S, Weigel NL, Gestwicki J, Trepel J, Neckers LM. Targeting the Hsp40/Hsp70 chaperone axis as a novel strategy to treat castration-resistant prostate cancer. Cancer Res. 2018 May 15. PMID: 29764864.
      View in: PubMed
    6. Taylor IR, Ahmad A, Wu T, Nordhues BA, Bhullar A, Gestwicki J, Zuiderweg ERP. The disorderly conduct of Hsc70 and its interaction with the Alzheimer's related Tau protein. J Biol Chem. 2018 May 15. PMID: 29764935.
      View in: PubMed
    7. Mok SA, Condello C, Freilich R, Gillies A, Arhar T, Oroz J, Kadavath H, Julien O, Assimon VA, Rauch JN, Dunyak BM, Lee J, Tsai FTF, Wilson MR, Zweckstetter M, Dickey CA, Gestwicki J. Mapping interactions with the chaperone network reveals factors that protect against tau aggregation. Nat Struct Mol Biol. 2018 May; 25(5):384-393. PMID: 29728653.
      View in: PubMed
    8. Ran X, Burchfiel ET, Dong B, Rettko NJ, Dunyak BM, Shao H, Thiele DJ, Gestwicki J. Rational design and screening of peptide-based inhibitors of heat shock factor 1 (HSF1). Bioorg Med Chem. 2018 Apr 07. PMID: 29661622.
      View in: PubMed
    9. Freilich R, Arhar T, Abrams JL, Gestwicki J. Protein-Protein Interactions in the Molecular Chaperone Network. Acc Chem Res. 2018 Apr 03. PMID: 29613769.
      View in: PubMed
    10. Ouimet CM, Dawod M, Grinias J, Assimon VA, Lodge J, Mapp AK, Gestwicki J, Kennedy RT. Protein cross-linking capillary electrophoresis at increased throughput for a range of protein-protein interactions. Analyst. 2018 Mar 22. PMID: 29565056.
      View in: PubMed
    11. Min SW, Sohn PD, Li Y, Devidze N, Johnson JR, Krogan NJ, Masliah E, Mok SA, Gestwicki J, Gan L. SIRT1 Deacetylates Tau and Reduces Pathogenic Tau Spread in a Mouse Model of Tauopathy. J Neurosci. 2018 Apr 11; 38(15):3680-3688. PMID: 29540553.
      View in: PubMed
    12. Taylor IR, Dunyak BM, Komiyama T, Shao H, Ran X, Assimon VA, Kalyanaraman C, Rauch JN, Jacobson MP, Zuiderweg ERP, Gestwicki J. High Throughput Screen for Inhibitors of Protein-Protein Interactions in a Reconstituted Heat Shock Protein 70 (Hsp70) Complex. J Biol Chem. 2018 Feb 02. PMID: 29414793.
      View in: PubMed
    13. Young ZT, Mok SA, Gestwicki J. Therapeutic Strategies for Restoring Tau Homeostasis. Cold Spring Harb Perspect Med. 2018 Jan 02; 8(1). PMID: 28159830.
      View in: PubMed
    14. Cesa LC, Shao H, Srinivasan SR, Tse E, Jain C, Zuiderweg ERP, Southworth DR, Mapp AK, Gestwicki J. X-linked inhibitor of apoptosis protein (XIAP) is a client of heat shock protein 70 (Hsp70) and a biomarker of its inhibition. J Biol Chem. 2018 Feb 16; 293(7):2370-2380. PMID: 29255093.
      View in: PubMed
    15. Srinivasan SR, Cesa LC, Li X, Julien O, Zhuang M, Shao H, Chung J, Maillard I, Wells JA, Duckett CS, Gestwicki J. Heat Shock Protein 70 (Hsp70) Suppresses RIP1-Dependent Apoptotic and Necroptotic Cascades. Mol Cancer Res. 2018 Jan; 16(1):58-68. PMID: 28970360.
      View in: PubMed
    16. Rauch JN, Olson SH, Gestwicki J. Interactions between Microtubule-Associated Protein Tau (MAPT) and Small Molecules. Cold Spring Harb Perspect Med. 2017 Jul 05; 7(7). PMID: 27940599.
      View in: PubMed
    17. Pricer R, Gestwicki J, Mapp AK. From Fuzzy to Function: The New Frontier of Protein-Protein Interactions. Acc Chem Res. 2017 Mar 21; 50(3):584-589. PMID: 28945413.
      View in: PubMed
    18. Zuiderweg ER, Hightower LE, Gestwicki J. The remarkable multivalency of the Hsp70 chaperones. Cell Stress Chaperones. 2017 Mar; 22(2):173-189. PMID: 28220454.
      View in: PubMed
    19. Rauch JN, Tse E, Freilich R, Mok SA, Makley LN, Southworth DR, Gestwicki J. BAG3 Is a Modular, Scaffolding Protein that physically Links Heat Shock Protein 70 (Hsp70) to the Small Heat Shock Proteins. J Mol Biol. 2017 01 06; 429(1):128-141. PMID: 27884606.
      View in: PubMed
    20. Costa MDC, Ashraf NS, Fischer S, Yang Y, Schapka E, Joshi G, McQuade TJ, Dharia RM, Dulchavsky M, Ouyang M, Cook D, Sun D, Larsen MJ, Gestwicki J, Todi SV, Ivanova MI, Paulson HL. Unbiased screen identifies aripiprazole as a modulator of abundance of the polyglutamine disease protein, ataxin-3. Brain. 2016 11 01; 139(11):2891-2908. PMID: 27645800.
      View in: PubMed
    21. Zuiderweg ER, Gestwicki J. Backbone and methyl resonance assignments of the 42 kDa human Hsc70 nucleotide binding domain in the ADP state. Biomol NMR Assign. 2017 04; 11(1):11-15. PMID: 27699616.
      View in: PubMed
    22. Gabai VL, Yaglom JA, Wang Y, Meng L, Shao H, Kim G, Colvin T, Gestwicki J, Sherman MY. Anticancer Effects of Targeting Hsp70 in Tumor Stromal Cells. Cancer Res. 2016 10 15; 76(20):5926-5932. PMID: 27503927.
      View in: PubMed
    23. Young ZT, Rauch JN, Assimon VA, Jinwal UK, Ahn M, Li X, Dunyak BM, Ahmad A, Carlson GA, Srinivasan SR, Zuiderweg ER, Dickey CA, Gestwicki J. Stabilizing the Hsp70-Tau Complex Promotes Turnover in Models of Tauopathy. Cell Chem Biol. 2016 Aug 18; 23(8):992-1001. PMID: 27499529; PMCID: PMC4992411 [Available on 08/18/17].
    24. Rauch JN, Zuiderweg ER, Gestwicki J. Non-canonical Interactions between Heat Shock Cognate Protein 70 (Hsc70) and Bcl2-associated Anthanogene (BAG) Co-Chaperones Are Important for Client Release. J Biol Chem. 2016 09 16; 291(38):19848-57. PMID: 27474739.
      View in: PubMed
    25. Ouimet CM, Shao H, Rauch JN, Dawod M, Nordhues B, Dickey CA, Gestwicki J, Kennedy RT. Protein Cross-Linking Capillary Electrophoresis for Protein-Protein Interaction Analysis. Anal Chem. 2016 Aug 16; 88(16):8272-8. PMID: 27434096.
      View in: PubMed
    26. Dunyak BM, Gestwicki J. Peptidyl-Proline Isomerases (PPIases): Targets for Natural Products and Natural Product-Inspired Compounds. J Med Chem. 2016 Nov 10; 59(21):9622-9644. PMID: 27409354.
      View in: PubMed
    27. Sabnis AJ, Guerriero CJ, Olivas V, Sayana A, Shue J, Flanagan J, Asthana S, Paton AW, Paton JC, Gestwicki J, Walter P, Weissman JS, Wipf P, Brodsky JL, Bivona TG. Combined chemical-genetic approach identifies cytosolic HSP70 dependence in rhabdomyosarcoma. Proc Natl Acad Sci U S A. 2016 08 09; 113(32):9015-20. PMID: 27450084; PMCID: PMC4987817 [Available on 02/09/17].
    28. Morozova K, Clement CC, Kaushik S, Stiller B, Arias E, Ahmad A, Rauch JN, Chatterjee V, Melis C, Scharf B, Gestwicki J, Cuervo AM, Zuiderweg ER, Santambrogio L. Structural and Biological Interaction of hsc-70 Protein with Phosphatidylserine in Endosomal Microautophagy. J Biol Chem. 2016 08 26; 291(35):18096-106. PMID: 27405763; PMCID: PMC5000059 [Available on 08/26/17].
    29. Martin MD, Baker JD, Suntharalingam A, Nordhues BA, Shelton LB, Zheng D, Sabbagh JJ, Haystead TA, Gestwicki J, Dickey CA. Inhibition of Both Hsp70 Activity and Tau Aggregation in Vitro Best Predicts Tau Lowering Activity of Small Molecules. ACS Chem Biol. 2016 07 15; 11(7):2041-8. PMID: 27177119.
      View in: PubMed
    30. Paatero AO, Kellosalo J, Dunyak BM, Almaliti J, Gestwicki J, Gerwick WH, Taunton J, Paavilainen VO. Apratoxin Kills Cells by Direct Blockade of the Sec61 Protein Translocation Channel. Cell Chem Biol. 2016 May 19; 23(5):561-566. PMID: 27203376.
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    31. Khachatoorian R, Riahi R, Ganapathy E, Shao H, Wheatley NM, Sundberg C, Jung CL, Ruchala P, Dasgupta A, Arumugaswami V, Gestwicki J, French SW. Allosteric heat shock protein 70 inhibitors block hepatitis C virus assembly. Int J Antimicrob Agents. 2016 Apr; 47(4):289-96. PMID: 27013001; PMCID: PMC4833571 [Available on 04/01/17].
    32. Li X, Shao H, Taylor IR, Gestwicki J. Targeting Allosteric Control Mechanisms in Heat Shock Protein 70 (Hsp70). Curr Top Med Chem. 2016; 16(25):2729-40. PMID: 27072701.
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    33. Klionsky DJ, Abdelmohsen K, Abe A, Abedin MJ, Abeliovich H, Acevedo Arozena A, Adachi H, Adams CM, Adams PD, Adeli K, Adhihetty PJ, Adler SG, Agam G, Agarwal R, Aghi MK, Agnello M, Agostinis P, Aguilar PV, Aguirre-Ghiso J, Airoldi EM, Ait-Si-Ali S, Akematsu T, Akporiaye ET, Al-Rubeai M, Albaiceta GM, Albanese C, Albani D, Albert ML, Aldudo J, Algül H, Alirezaei M, Alloza I, Almasan A, Almonte-Beceril M, Alnemri ES, Alonso C, Altan-Bonnet N, Altieri DC, Alvarez S, Alvarez-Erviti L, Alves S, Amadoro G, Amano A, Amantini C, Ambrosio S, Amelio I, Amer AO, Amessou M, Amon A, An Z, Anania FA, Andersen SU, Andley UP, Andreadi CK, Andrieu-Abadie N, Anel A, Ann DK, Anoopkumar-Dukie S, Antonioli M, Aoki H, Apostolova N, Aquila S, Aquilano K, Araki K, Arama E, Aranda A, Araya J, Arcaro A, Arias E, Arimoto H, Ariosa AR, Armstrong JL, Arnould T, Arsov I, Asanuma K, Askanas V, Asselin E, Atarashi R, Atherton SS, Atkin JD, Attardi LD, Auberger P, Auburger G, Aurelian L, Autelli R, Avagliano L, Avantaggiati ML, Avrahami L, Awale S, Azad N, Bachetti T, Backer JM, Bae DH, Bae JS, Bae ON, Bae SH, Baehrecke EH, Baek SH, Baghdiguian S, Bagniewska-Zadworna A, Bai H, Bai J, Bai XY, Bailly Y, Balaji KN, Balduini W, Ballabio A, Balzan R, Banerjee R, Bánhegyi G, Bao H, Barbeau B, Barrachina MD, Barreiro E, Bartel B, Bartolomé A, Bassham DC, Bassi MT, Bast RC, Basu A, Batista MT, Batoko H, Battino M, Bauckman K, Baumgarner BL, Bayer KU, Beale R, Beaulieu JF, Beck GR, Becker C, Beckham JD, Bédard PA, Bednarski PJ, Begley TJ, Behl C, Behrends C, Behrens GM, Behrns KE, Bejarano E, Belaid A, Belleudi F, Bénard G, Berchem G, Bergamaschi D, Bergami M, Berkhout B, Berliocchi L, Bernard A, Bernard M, Bernassola F, Bertolotti A, Bess AS, Besteiro S, Bettuzzi S, Bhalla S, Bhattacharyya S, Bhutia SK, Biagosch C, Bianchi MW, Biard-Piechaczyk M, Billes V, Bincoletto C, Bingol B, Bird SW, Bitoun M, Bjedov I, Blackstone C, Blanc L, Blanco GA, Blomhoff HK, Boada-Romero E, Böckler S, Boes M, Boesze-Battaglia K, Boise LH, Bolino A, Boman A, Bonaldo P, Bordi M, Bosch J, Botana LM, Botti J, Bou G, Bouché M, Bouchecareilh M, Boucher MJ, Boulton ME, Bouret SG, Boya P, Boyer-Guittaut M, Bozhkov PV, Brady N, Braga VM, Brancolini C, Braus GH, Bravo-San Pedro JM, Brennan LA, Bresnick EH, Brest P, Bridges D, Bringer MA, Brini M, Brito GC, Brodin B, Brookes PS, Brown EJ, Brown K, Broxmeyer HE, Bruhat A, Brum PC, Brumell JH, Brunetti-Pierri N, Bryson-Richardson RJ, Buch S, Buchan AM, Budak H, Bulavin DV, Bultman SJ, Bultynck G, Bumbasirevic V, Burelle Y, Burke RE, Burmeister M, Bütikofer P, Caberlotto L, Cadwell K, Cahova M, Cai D, Cai J, Cai Q, Calatayud S, Camougrand N, Campanella M, Campbell GR, Campbell M, Campello S, Candau R, Caniggia I, Cantoni L, Cao L, Caplan AB, Caraglia M, Cardinali C, Cardoso SM, Carew JS, Carleton LA, Carlin CR, Carloni S, Carlsson SR, Carmona-Gutierrez D, Carneiro LA, Carnevali O, Carra S, Carrier A, Carroll B, Casas C, Casas J, Cassinelli G, Castets P, Castro-Obregon S, Cavallini G, Ceccherini I, Cecconi F, Cederbaum AI, Ceña V, Cenci S, Cerella C, Cervia D, Cetrullo S, Chaachouay H, Chae HJ, Chagin AS, Chai CY, Chakrabarti G, Chamilos G, Chan EY, Chan MT, Chandra D, Chandra P, Chang CP, Chang RC, Chang TY, Chatham JC, Chatterjee S, Chauhan S, Che Y, Cheetham ME, Cheluvappa R, Chen CJ, Chen G, Chen GC, Chen G, Chen H, Chen JW, Chen JK, Chen M, Chen M, Chen P, Chen Q, Chen Q, Chen SD, Chen S, Chen SS, Chen W, Chen WJ, Chen WQ, Chen W, Chen X, Chen YH, Chen YG, Chen Y, Chen Y, Chen Y, Chen YJ, Chen YQ, Chen Y, Chen Z, Chen Z, Cheng A, Cheng CH, Cheng H, Cheong H, Cherry S, Chesney J, Cheung CH, Chevet E, Chi HC, Chi SG, Chiacchiera F, Chiang HL, Chiarelli R, Chiariello M, Chieppa M, Chin LS, Chiong M, Chiu GN, Cho DH, Cho SG, Cho WC, Cho YY, Cho YS, Choi AM, Choi EJ, Choi EK, Choi J, Choi ME, Choi SI, Chou TF, Chouaib S, Choubey D, Choubey V, Chow KC, Chowdhury K, Chu CT, Chuang TH, Chun T, Chung H, Chung T, Chung YL, Chwae YJ, Cianfanelli V, et al. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy. 2016; 12(1):1-222. PMID: 26799652.
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    34. Assimon VA, Southworth DR, Gestwicki J. Specific Binding of Tetratricopeptide Repeat Proteins to Heat Shock Protein 70 (Hsp70) and Heat Shock Protein 90 (Hsp90) Is Regulated by Affinity and Phosphorylation. Biochemistry. 2015 Dec 08; 54(48):7120-31. PMID: 26565746; PMCID: PMC4714923 [Available on 12/08/16].
    35. Taguwa S, Maringer K, Li X, Bernal-Rubio D, Rauch JN, Gestwicki J, Andino R, Fernandez-Sesma A, Frydman J. Defining Hsp70 Subnetworks in Dengue Virus Replication Reveals Key Vulnerability in Flavivirus Infection. Cell. 2015 Nov 19; 163(5):1108-1123. PMID: 26582131; PMCID: PMC4869517 [Available on 11/19/16].
    36. Makley LN, McMenimen KA, DeVree BT, Goldman JW, McGlasson BN, Rajagopal P, Dunyak BM, McQuade TJ, Thompson AD, Sunahara R, Klevit RE, Andley UP, Gestwicki J. Pharmacological chaperone for a-crystallin partially restores transparency in cataract models. Science. 2015 Nov 06; 350(6261):674-7. PMID: 26542570; PMCID: PMC4725592 [Available on 11/06/16].
    37. Min SW, Chen X, Tracy TE, Li Y, Zhou Y, Wang C, Shirakawa K, Minami SS, Defensor E, Mok SA, Sohn PD, Schilling B, Cong X, Ellerby L, Gibson BW, Johnson J, Krogan N, Shamloo M, Gestwicki J, Masliah E, Verdin E, Gan L. Critical role of acetylation in tau-mediated neurodegeneration and cognitive deficits. Nat Med. 2015 Oct; 21(10):1154-62. PMID: 26390242.
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    38. Dunyak BM, Nakamura RL, Frankel AD, Gestwicki J. Selective Targeting of Cells via Bispecific Molecules That Exploit Coexpression of Two Intracellular Proteins. ACS Chem Biol. 2015 Nov 20; 10(11):2441-7. PMID: 26322864; PMCID: PMC4714926 [Available on 11/20/16].
    39. Cesa LC, Mapp AK, Gestwicki J. Direct and Propagated Effects of Small Molecules on Protein-Protein Interaction Networks. Front Bioeng Biotechnol. 2015; 3:119. PMID: 26380257; PMCID: PMC4547496.
    40. Guan S, Trnka MJ, Bushnell DA, Robinson PJ, Gestwicki J, Burlingame AL. Deconvolution method for specific and nonspecific binding of ligand to multiprotein complex by native mass spectrometry. Anal Chem. 2015 Aug 18; 87(16):8541-6. PMID: 26189511; PMCID: PMC4714924.
    41. Fontaine SN, Martin MD, Akoury E, Assimon VA, Borysov S, Nordhues BA, Sabbagh JJ, Cockman M, Gestwicki J, Zweckstetter M, Dickey CA. The active Hsc70/tau complex can be exploited to enhance tau turnover without damaging microtubule dynamics. Hum Mol Genet. 2015 Jul 15; 24(14):3971-81. PMID: 25882706; PMCID: PMC4560066.
    42. Fontaine SN, Rauch JN, Nordhues BA, Assimon VA, Stothert AR, Jinwal UK, Sabbagh JJ, Chang L, Stevens SM, Zuiderweg ER, Gestwicki J, Dickey CA. Isoform-selective Genetic Inhibition of Constitutive Cytosolic Hsp70 Activity Promotes Client Tau Degradation Using an Altered Co-chaperone Complement. J Biol Chem. 2015 May 22; 290(21):13115-27. PMID: 25864199; PMCID: PMC4505567.
    43. Assimon VA, Shao H, Garneau-Tsodikova S, Gestwicki J. Concise Synthesis of Spergualin-Inspired Molecules With Broad-Spectrum Antibiotic Activity. Medchemcomm. 2015 May 01; 6(5):912-918. PMID: 27087913.
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    44. Martin MD, Calcul L, Smith C, Jinwal UK, Fontaine SN, Darling A, Seeley K, Wojtas L, Narayan M, Gestwicki J, Smith GR, Reitz AB, Baker BJ, Dickey CA. Synthesis, stereochemical analysis, and derivatization of myricanol provide new probes that promote autophagic tau clearance. ACS Chem Biol. 2015 Apr 17; 10(4):1099-109. PMID: 25588114; PMCID: PMC4971885.
    45. Li X, Colvin T, Rauch JN, Acosta-Alvear D, Kampmann M, Dunyak B, Hann B, Aftab BT, Murnane M, Cho M, Walter P, Weissman JS, Sherman MY, Gestwicki J. Validation of the Hsp70-Bag3 protein-protein interaction as a potential therapeutic target in cancer. Mol Cancer Ther. 2015 Mar; 14(3):642-8. PMID: 25564440; PMCID: PMC4456214.
    46. Evans CG, Gestwicki J. Correction to enantioselective organocatalytic hantzsch synthesis of polyhydroquinolines. Org Lett. 2014 Nov 21; 16(22):6040. PMID: 25275212; PMCID: PMC4198511.
    47. Pratt WB, Gestwicki J, Osawa Y, Lieberman AP. Targeting Hsp90/Hsp70-based protein quality control for treatment of adult onset neurodegenerative diseases. Annu Rev Pharmacol Toxicol. 2015; 55:353-71. PMID: 25292434; PMCID: PMC4372135.
    48. Jaeger AM, Makley LN, Gestwicki J, Thiele DJ. Genomic heat shock element sequences drive cooperative human heat shock factor 1 DNA binding and selectivity. J Biol Chem. 2014 Oct 31; 289(44):30459-69. PMID: 25204655; PMCID: PMC4215228.
    49. Selenica ML, Davtyan H, Housley SB, Blair LJ, Gillies A, Nordhues BA, Zhang B, Liu J, Gestwicki J, Lee DC, Gordon MN, Morgan D, Dickey CA. Epitope analysis following active immunization with tau proteins reveals immunogens implicated in tau pathogenesis. J Neuroinflammation. 2014 Sep 03; 11:152. PMID: 25183004; PMCID: PMC4167523.
    50. Rauch JN, Gestwicki J. Rehabilitating mutant GCase. Chem Biol. 2014 Aug 14; 21(8):919-20. PMID: 25126987.
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    51. Ung PM, Dunbar JB, Gestwicki J, Carlson HA. An allosteric modulator of HIV-1 protease shows equipotent inhibition of wild-type and drug-resistant proteases. J Med Chem. 2014 Aug 14; 57(15):6468-78. PMID: 25062388; PMCID: PMC4136727.
    52. Colvin TA, Gabai VL, Gong J, Calderwood SK, Li H, Gummuluru S, Matchuk ON, Smirnova SG, Orlova NV, Zamulaeva IA, Garcia-Marcos M, Li X, Young ZT, Rauch JN, Gestwicki J, Takayama S, Sherman MY. Hsp70-Bag3 interactions regulate cancer-related signaling networks. Cancer Res. 2014 Sep 01; 74(17):4731-40. PMID: 24994713; PMCID: PMC4174322.
    53. Pratt WB, Morishima Y, Gestwicki J, Lieberman AP, Osawa Y. A model in which heat shock protein 90 targets protein-folding clefts: rationale for a new approach to neuroprotective treatment of protein folding diseases. Exp Biol Med (Maywood). 2014 Nov; 239(11):1405-13. PMID: 24990484; PMCID: PMC4318483.
    54. Walter GM, Raveh A, Mok SA, McQuade TJ, Arevang CJ, Schultz PJ, Smith MC, Asare S, Cruz PG, Wisen S, Matainaho T, Sherman DH, Gestwicki J. High-throughput screen of natural product extracts in a yeast model of polyglutamine proteotoxicity. Chem Biol Drug Des. 2014 Apr; 83(4):440-9. PMID: 24636344; PMCID: PMC4068144.
    55. Connarn JN, Assimon VA, Reed RA, Tse E, Southworth DR, Zuiderweg ER, Gestwicki J, Sun D. The molecular chaperone Hsp70 activates protein phosphatase 5 (PP5) by binding the tetratricopeptide repeat (TPR) domain. J Biol Chem. 2014 Jan 31; 289(5):2908-17. PMID: 24327656; PMCID: PMC3908423.
    56. Rauch JN, Gestwicki J. Binding of human nucleotide exchange factors to heat shock protein 70 (Hsp70) generates functionally distinct complexes in vitro. J Biol Chem. 2014 Jan 17; 289(3):1402-14. PMID: 24318877; PMCID: PMC3894324.
    57. Ung PM, Thompson AD, Chang L, Gestwicki J, Carlson HA. Identification of key hinge residues important for nucleotide-dependent allostery in E. coli Hsp70/DnaK. PLoS Comput Biol. 2013; 9(11):e1003279. PMID: 24277995; PMCID: PMC3836694.
    58. Li X, Srinivasan SR, Connarn J, Ahmad A, Young ZT, Kabza AM, Zuiderweg ER, Sun D, Gestwicki J. Analogs of the Allosteric Heat Shock Protein 70 (Hsp70) Inhibitor, MKT-077, as Anti-Cancer Agents. ACS Med Chem Lett. 2013 Nov 14; 4(11). PMID: 24312699.
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    59. Rauch JN, Nie J, Buchholz TJ, Gestwicki J, Kennedy RT. Development of a capillary electrophoresis platform for identifying inhibitors of protein-protein interactions. Anal Chem. 2013 Oct 15; 85(20):9824-31. PMID: 24060167; PMCID: PMC3845662.
    60. Smith MC, Scaglione KM, Assimon VA, Patury S, Thompson AD, Dickey CA, Southworth DR, Paulson HL, Gestwicki J, Zuiderweg ER. The E3 ubiquitin ligase CHIP and the molecular chaperone Hsc70 form a dynamic, tethered complex. Biochemistry. 2013 Aug 13; 52(32):5354-64. PMID: 23865999; PMCID: PMC3856692.
    61. Cesa LC, Patury S, Komiyama T, Ahmad A, Zuiderweg ERP, Gestwicki J. Inhibitors of difficult protein-protein interactions identified by high-throughput screening of multiprotein complexes. ACS Chem Biol. 2013 Sep 20; 8(9):1988-1997. PMID: 23819499; PMCID: PMC3783524.
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    136. Gestwicki J, Crabtree GR, Graef IA. Harnessing chaperones to generate small-molecule inhibitors of amyloid beta aggregation. Science. 2004 Oct 29; 306(5697):865-9. PMID: 15514157.
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    137. Chang CP, Neilson JR, Bayle JH, Gestwicki J, Kuo A, Stankunas K, Graef IA, Crabtree GR. A field of myocardial-endocardial NFAT signaling underlies heart valve morphogenesis. Cell. 2004 Sep 03; 118(5):649-63. PMID: 15339668.
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    138. Stankunas K, Bayle JH, Gestwicki J, Lin YM, Wandless TJ, Crabtree GR. Conditional protein alleles using knockin mice and a chemical inducer of dimerization. Mol Cell. 2003 Dec; 12(6):1615-24. PMID: 14690613.
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    139. Gestwicki J, Cairo CW, Borrok MJ, Kiessling LL. Visualization and characterization of receptor clusters by transmission electron microscopy. Methods Enzymol. 2003; 362:301-12. PMID: 12968372.
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    140. Gestwicki J, Cairo CW, Strong LE, Oetjen KA, Kiessling LL. Influencing receptor-ligand binding mechanisms with multivalent ligand architecture. J Am Chem Soc. 2002 Dec 18; 124(50):14922-33. PMID: 12475334.
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    141. Lamanna AC, Gestwicki J, Strong LE, Borchardt SL, Owen RM, Kiessling LL. Conserved amplification of chemotactic responses through chemoreceptor interactions. J Bacteriol. 2002 Sep; 184(18):4981-7. PMID: 12193613; PMCID: PMC135308.
    142. Owen RM, Gestwicki J, Young T, Kiessling LL. Synthesis and applications of end-labeled neoglycopolymers. Org Lett. 2002 Jul 11; 4(14):2293-6. PMID: 12098230.
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    143. Gestwicki J, Cairo CW, Mann DA, Owen RM, Kiessling LL. Selective immobilization of multivalent ligands for surface plasmon resonance and fluorescence microscopy. Anal Biochem. 2002 Jun 15; 305(2):149-55. PMID: 12054443.
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    144. Cairo CW, Gestwicki J, Kanai M, Kiessling LL. Control of multivalent interactions by binding epitope density. J Am Chem Soc. 2002 Feb 27; 124(8):1615-9. PMID: 11853434.
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    145. Gestwicki J, Strong LE, Cairo CW, Boehm FJ, Kiessling LL. Cell aggregation by scaffolded receptor clusters. Chem Biol. 2002 Feb; 9(2):163-9. PMID: 11880031.
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    146. Gestwicki J, Kiessling LL. Inter-receptor communication through arrays of bacterial chemoreceptors. Nature. 2002 Jan 03; 415(6867):81-4. PMID: 11780121.
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    147. Gestwicki J, Hsieh HV, Pitner JB. Using receptor conformational change to detect low molecular weight analytes by surface plasmon resonance. Anal Chem. 2001 Dec 01; 73(23):5732-7. PMID: 11774914.
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    148. Gestwicki J, Strong LE, Borchardt SL, Cairo CW, Schnoes AM, Kiessling LL. Designed potent multivalent chemoattractants for Escherichia coli. Bioorg Med Chem. 2001 Sep; 9(9):2387-93. PMID: 11553480.
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    149. Gestwicki J, Strong LE, Kiessling LL. Visualization of Single Multivalent Receptor-Ligand Complexes by Transmission Electron Microscopy The authors thank Colleen Lavin (UW Madison, Microscopy Resource) and Kim Dickson for experimental support. This work was supported in part by the NIH (GM 55984). J.E.G. acknowledges the NIH Biotechnology Training Grant for support (T32GM08349). L.E.S. was supported by an NIH predoctoral fellowship (GM 18750). Angew Chem Int Ed Engl. 2000 Dec 15; 39(24):4567-4570. PMID: 11169672.
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    150. Kiessling LL, Gestwicki J, Strong LE. Synthetic multivalent ligands in the exploration of cell-surface interactions. Curr Opin Chem Biol. 2000 Dec; 4(6):696-703. PMID: 11102876.
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    151. Gestwicki J, Lamanna AC, Harshey RM, McCarter LL, Kiessling LL, Adler J. Evolutionary conservation of methyl-accepting chemotaxis protein location in Bacteria and Archaea. J Bacteriol. 2000 Nov; 182(22):6499-502. PMID: 11053396; PMCID: PMC94798.
    152. Hoag KA, Clise-Dwyer K, Lim YH, Nashold FE, Gestwicki J, Cancro MP, Hayes CE. A quantitative-trait locus controlling peripheral B-cell deficiency maps to mouse Chromosome 15. Immunogenetics. 2000 Sep; 51(11):924-9. PMID: 11003386.
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    153. Gestwicki J, Strong LE, Kiessling LL. Tuning chemotactic responses with synthetic multivalent ligands. Chem Biol. 2000 Aug; 7(8):583-91. PMID: 11048949.
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    154. Maki N, Gestwicki J, Lake EM, Kiessling LL, Adler J. Motility and chemotaxis of filamentous cells of Escherichia coli. J Bacteriol. 2000 Aug; 182(15):4337-42. PMID: 10894745; PMCID: PMC101954.
    155. Kabza KG, Chapados BR, Gestwicki J, McGrath JL. Microwave-induced esterification using heterogeneous acid catalyst in a low dielectric constant medium J Org Chem. 2000 Feb 25; 65(4):1210-4. PMID: 10814074.
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    156. Gordon EJ, Gestwicki J, Strong LE, Kiessling LL. Synthesis of end-labeled multivalent ligands for exploring cell-surface-receptor-ligand interactions. Chem Biol. 2000 Jan; 7(1):9-16. PMID: 10662681.
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