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Arun Wiita, MD, PhD

Title(s)Associate Professor, Laboratory Medicine
SchoolSchool of Medicine
Phone415-514-6238
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    Collapse Biography 
    Collapse Education and Training
    Princeton UniversityA.B.2002Chemistry
    Columbia UniversityMD/PhD2009Medicine/Biophysics
    UCSFResidency2012Laboratory Medicine
    UCSFPost-doc2014Pharmaceutical Chemistry
    Collapse Awards and Honors
    Gabrielle's Angels Foundation for Cancer Research2017Janet Rowley Memorial Medical Research Award
    NIH Director's Common Fund2016New Innovator Award (DP2)
    Doris Duke Charitable Foundation2016Clinical Scientist Development Award
    Damon Runyon Cancer Research Foundation2015Dale Frey Breakthrough Award
    UCSF-American Cancer Society2015Individual Research Award
    NIH-NCI2014  - 2019K08 Mentored Clinical Scientist Award
    Damon Runyon Cancer Research Foundation2012  - 2014Post-doctoral Fellowship
    Columbia University2008Dean's Award for Excellence in Research (top PhD thesis prize)

    Collapse Overview 
    Collapse Overview
    My research laboratory spans a number of areas within genomics, proteomics, and hematologic malignancies. One major area of research involves the use of targeted, quantitative proteomics to answer important biological questions in myeloma and B-cell leukemia. We are particularly interested in questions related to translational regulation after cancer therapy as well as how clinically-relevant genomic alterations result in phenotypic changes at the level of the proteome. Furthermore, my group includes the UCSF Stephen and Nancy Grand Multiple Myeloma Translational Initiative Laboratory. In this role, we collaborate with industry and academic partners to advance preclinical evaluation of novel myeloma therapeutics using a suite of in vitro and in vivo approaches.

    In my clinical work, I am Assistant Director of the UCSF Clinical Cytogenetics laboratory. In this role, I interpret molecular genetic testing for the prenatal and postnatal diagnosis of microdeletion and microduplication syndromes. In this area we have translational interests in emerging technologies for determination of DNA copy number variation, integration of single-nucleotide variants and CNV interpretation, and cell-free DNA for prenatal testing. In my research laboratory we are also using quantitative proteomics to evaluate the role of protein homeostasis in determining the phenotypic effects of CNVs, as well using CRISPR/Cas9 genome engineering to generate new functional models of syndromic CNVs.

    Collapse Research 
    Collapse Research Activities and Funding
    Immuno Oncology Product for Multiple Myeloma: g-NK Cellular Therapy to Optimize Monoclonal Antibody Efficacy in Treating Multiple
    NIH/NCI R43CA235786May 15, 2019 - Feb 29, 2020
    Role: Co-Principal Investigator
    Exploiting myeloma proteome remodeling to extend proteasome inhibitor efficacy
    NIH/NCI R01CA226851Mar 1, 2018 - Feb 28, 2023
    Role: Principal Investigator
    In vivo monitoring of oxidative protein folding through time-resolved quantitative mass spectrometry
    NIH/NIGMS DP2GM123500Sep 30, 2016 - May 31, 2021
    Role: Principal Investigator
    Global Assessment of Myeloma Response to Chemotherapy
    NIH/NCI K08CA184116Sep 16, 2014 - Aug 31, 2019
    Role: Principal Investigator

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    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
    List All   |   Timeline
    1. Crottès D, Lin YT, Peters CJ, Gilchrist JM, Wiita AP, Jan YN, Jan LY. TMEM16A controls EGF-induced calcium signaling implicated in pancreatic cancer prognosis. Proc Natl Acad Sci U S A. 2019 Jun 25; 116(26):13026-13035. PMID: 31182586.
      View in: PubMed
    2. Trinklein ND, Pham D, Schellenberger U, Buelow B, Boudreau A, Choudhry P, Clarke SC, Dang K, Harris KE, Iyer S, Jorgensen B, Pratap PP, Rangaswamy US, Ugamraj HS, Vafa O, Wiita AP, van Schooten W, Buelow R, Force Aldred S. Efficient tumor killing and minimal cytokine release with novel T-cell agonist bispecific antibodies. MAbs. 2019 Feb 20; 1-14. PMID: 30698484.
      View in: PubMed
    3. Spector JD, Wiita AP. ClinTAD: a tool for copy number variant interpretation in the context of topologically associated domains. J Hum Genet. 2019 May; 64(5):437-443. PMID: 30765865.
      View in: PubMed
    4. Abrahams CL, Li X, Embry M, Yu A, Krimm S, Krueger S, Greenland NY, Wen KW, Jones C, DeAlmeida V, Solis WA, Matheny S, Kline T, Yam AY, Stafford R, Wiita AP, Hallam T, Lupher M, Molina A. Targeting CD74 in multiple myeloma with the novel, site-specific antibody-drug conjugate STRO-001. Oncotarget. 2018 Dec 28; 9(102):37700-37714. PMID: 30701025.
      View in: PubMed
    5. Choudhry P, Galligan D, Wiita AP. Seeking Convergence and Cure with New Myeloma Therapies. Trends Cancer. 2018 08; 4(8):567-582. PMID: 30064664.
      View in: PubMed
    6. Lam C, Ferguson ID, Mariano MC, Lin YT, Murnane M, Liu H, Smith GA, Wong SW, Taunton J, Liu JO, Mitsiades CS, Hann BC, Aftab BT, Wiita AP. Repurposing tofacitinib as an anti-myeloma therapeutic to reverse growth-promoting effects of the bone marrow microenvironment. Haematologica. 2018 Jul; 103(7):1218-1228. PMID: 29622655.
      View in: PubMed
    7. Goode B, Mondal G, Hyun M, Ruiz DG, Lin YH, Van Ziffle J, Joseph NM, Onodera C, Talevich E, Grenert JP, Hewedi IH, Snuderl M, Brat DJ, Kleinschmidt-DeMasters BK, Rodriguez FJ, Louis DN, Yong WH, Lopes MB, Rosenblum MK, Butowski N, Tihan T, Bollen AW, Phillips JJ, Wiita AP, Yeh I, Jacobson MP, Bastian BC, Perry A, Solomon DA. A recurrent kinase domain mutation in PRKCA defines chordoid glioma of the third ventricle. Nat Commun. 2018 02 23; 9(1):810. PMID: 29476136.
      View in: PubMed
    8. Deshpande A, Yadav S, Dao DQ, Wu ZY, Hokanson KC, Cahill MK, Wiita AP, Jan YN, Ullian EM, Weiss LA. Cellular Phenotypes in Human iPSC-Derived Neurons from a Genetic Model of Autism Spectrum Disorder. Cell Rep. 2017 Dec 05; 21(10):2678-2687. PMID: 29212016.
      View in: PubMed
    9. Le Moigne R, Aftab BT, Djakovic S, Dhimolea E, Valle E, Murnane M, King EM, Soriano F, Menon MK, Wu ZY, Wong ST, Lee GJ, Yao B, Wiita AP, Lam C, Rice J, Wang J, Chesi M, Bergsagel PL, Kraus M, Driessen C, Kiss von Soly S, Yakes FM, Wustrow D, Shawver L, Zhou HJ, Martin TG, Wolf JL, Mitsiades CS, Anderson DJ, Rolfe M. The p97 Inhibitor CB-5083 Is a Unique Disrupter of Protein Homeostasis in Models of Multiple Myeloma. Mol Cancer Ther. 2017 11; 16(11):2375-2386. PMID: 28878026.
      View in: PubMed
    10. Liu TY, Huang HH, Wheeler D, Xu Y, Wells JA, Song YS, Wiita AP. Time-Resolved Proteomics Extends Ribosome Profiling-Based Measurements of Protein Synthesis Dynamics. Cell Syst. 2017 Jun 28; 4(6):636-644.e9. PMID: 28578850.
      View in: PubMed
    11. Qi Z, Madaan S, Chetty S, Yu J, Wiita AP. False negative fetal cell free DNA screening for microdeletion syndromes in the presence of an unbalanced translocation involving monosomy 4p. Prenat Diagn. 2017 04; 37(4):420-422. PMID: 28207935.
      View in: PubMed
    12. Sherbenou DW, Aftab BT, Su Y, Behrens CR, Wiita A, Logan AC, Acosta-Alvear D, Hann BC, Walter P, Shuman MA, Wu X, Atkinson JP, Wolf JL, Martin TG, Liu B. Antibody-drug conjugate targeting CD46 eliminates multiple myeloma cells. J Clin Invest. 2016 12 01; 126(12):4640-4653. PMID: 27841764.
      View in: PubMed
    13. Mather CA, Qi Z, Wiita AP. False positive cell free DNA screening for microdeletions due to non-pathogenic copy number variants. Prenat Diagn. 2016 Jun; 36(6):584-6. PMID: 27061292.
      View in: PubMed
    14. Ori-McKenney KM, McKenney RJ, Huang HH, Li T, Meltzer S, Jan LY, Vale RD, Wiita AP, Jan YN. Phosphorylation of ß-Tubulin by the Down Syndrome Kinase, Minibrain/DYRK1a, Regulates Microtubule Dynamics and Dendrite Morphogenesis. Neuron. 2016 05 04; 90(3):551-63. PMID: 27112495; PMCID: PMC4860041 [Available on 05/04/17].
    15. Julien O, Zhuang M, Wiita AP, O'Donoghue AJ, Knudsen GM, Craik CS, Wells JA. Quantitative MS-based enzymology of caspases reveals distinct protein substrate specificities, hierarchies, and cellular roles. Proc Natl Acad Sci U S A. 2016 Apr 05; 113(14):E2001-10. PMID: 27006500; PMCID: PMC4833263 [Available on 10/05/16].
    16. Wiita AP, Hsu GW, Lu CM, Esensten JH, Wells JA. Circulating proteolytic signatures of chemotherapy-induced cell death in humans discovered by N-terminal labeling. Proc Natl Acad Sci U S A. 2014 May 27; 111(21):7594-9. PMID: 24821784; PMCID: PMC4040605.
    17. Carr SA, Abbatiello SE, Ackermann BL, Borchers C, Domon B, Deutsch EW, Grant RP, Hoofnagle AN, Hüttenhain R, Koomen JM, Liebler DC, Liu T, MacLean B, Mani DR, Mansfield E, Neubert H, Paulovich AG, Reiter L, Vitek O, Aebersold R, Anderson L, Bethem R, Blonder J, Boja E, Botelho J, Boyne M, Bradshaw RA, Burlingame AL, Chan D, Keshishian H, Kuhn E, Kinsinger C, Lee JS, Lee SW, Moritz R, Oses-Prieto J, Rifai N, Ritchie J, Rodriguez H, Srinivas PR, Townsend RR, Van Eyk J, Whiteley G, Wiita A, Weintraub S. Targeted peptide measurements in biology and medicine: best practices for mass spectrometry-based assay development using a fit-for-purpose approach. Mol Cell Proteomics. 2014 Mar; 13(3):907-17. PMID: 24443746; PMCID: PMC3945918.
    18. Wiita AP, Seaman JE, Wells JA. Global analysis of cellular proteolysis by selective enzymatic labeling of protein N-termini. Methods Enzymol. 2014; 544:327-58. PMID: 24974296; PMCID: PMC4260929.
    19. Wiita AP, Ziv E, Wiita PJ, Urisman A, Julien O, Burlingame AL, Weissman JS, Wells JA. Global cellular response to chemotherapy-induced apoptosis. Elife. 2013 Oct 29; 2:e01236. PMID: 24171104; PMCID: PMC3808542.
    20. Wiita AP, Nambiar A. Longitudinal management with crossmatch-compatible platelets for refractory patients: alloimmunization, response to transfusion, and clinical outcomes (CME). Transfusion. 2012 Oct; 52(10):2146-54. PMID: 23113654.
      View in: PubMed
    21. Wiita AP, Roubinian N, Khan Y, Chin-Hong PV, Singer JP, Golden JA, Miller S. Cytomegalovirus disease and infection in lung transplant recipients in the setting of planned indefinite valganciclovir prophylaxis. Transpl Infect Dis. 2012 Jun; 14(3):248-58. PMID: 22385394.
      View in: PubMed
    22. Wiita AP, Schrijver I. Clinical application of high throughput molecular screening techniques for pharmacogenomics. Pharmgenomics Pers Med. 2011; 4:109-21. PMID: 23226057.
      View in: PubMed
    23. Perez-Jimenez R, Li J, Kosuri P, Sanchez-Romero I, Wiita AP, Rodriguez-Larrea D, Chueca A, Holmgren A, Miranda-Vizuete A, Becker K, Cho SH, Beckwith J, Gelhaye E, Jacquot JP, Gaucher EA, Gaucher E, Sanchez-Ruiz JM, Berne BJ, Fernandez JM. Diversity of chemical mechanisms in thioredoxin catalysis revealed by single-molecule force spectroscopy. Nat Struct Mol Biol. 2009 Aug; 16(8):890-6. PMID: 19597482.
      View in: PubMed
    24. Perez-Jimenez R, Wiita AP, Rodriguez-Larrea D, Kosuri P, Gavira JA, Sanchez-Ruiz JM, Fernandez JM. Force-clamp spectroscopy detects residue co-evolution in enzyme catalysis. J Biol Chem. 2008 Oct 03; 283(40):27121-9. PMID: 18687682.
      View in: PubMed
    25. Koti Ainavarapu SR, Wiita AP, Dougan L, Uggerud E, Fernandez JM. Single-molecule force spectroscopy measurements of bond elongation during a bimolecular reaction. J Am Chem Soc. 2008 May 21; 130(20):6479-87. PMID: 18433129.
      View in: PubMed
    26. Ainavarapu SR, Wiita AP, Huang HH, Fernandez JM. A single-molecule assay to directly identify solvent-accessible disulfide bonds and probe their effect on protein folding. J Am Chem Soc. 2008 Jan 16; 130(2):436-7. PMID: 18088123.
      View in: PubMed
    27. Szoszkiewicz R, Ainavarapu SR, Wiita AP, Perez-Jimenez R, Sanchez-Ruiz JM, Fernandez JM. Dwell time analysis of a single-molecule mechanochemical reaction. Langmuir. 2008 Feb 19; 24(4):1356-64. PMID: 17999545.
      View in: PubMed
    28. Wiita AP, Perez-Jimenez R, Walther KA, Gräter F, Berne BJ, Holmgren A, Sanchez-Ruiz JM, Fernandez JM. Probing the chemistry of thioredoxin catalysis with force. Nature. 2007 Nov 01; 450(7166):124-7. PMID: 17972886.
      View in: PubMed
    29. Ainavarapu SR, Brujic J, Huang HH, Wiita AP, Lu H, Li L, Walther KA, Carrion-Vazquez M, Li H, Fernandez JM. Contour length and refolding rate of a small protein controlled by engineered disulfide bonds. Biophys J. 2007 Jan 01; 92(1):225-33. PMID: 17028145; PMCID: PMC1697845.
    30. Wiita AP, Ainavarapu SR, Huang HH, Fernandez JM. Force-dependent chemical kinetics of disulfide bond reduction observed with single-molecule techniques. Proc Natl Acad Sci U S A. 2006 May 09; 103(19):7222-7. PMID: 16645035; PMCID: PMC1464324.
    31. Kipp RA, Case MA, Wist AD, Cresson CM, Carrell M, Griner E, Wiita A, Albiniak PA, Chai J, Shi Y, Semmelhack MF, McLendon GL. Molecular targeting of inhibitor of apoptosis proteins based on small molecule mimics of natural binding partners. Biochemistry. 2002 Jun 11; 41(23):7344-9. PMID: 12044166.
      View in: PubMed