Jim Wells, PhD
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Title | Professor |
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School | UCSF School of Pharmacy |
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Department | Pharmaceutical Chemistry |
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Address | 1700 4th St San Francisco CA 94158
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Phone | 415-514-4498 |
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vCard | Download vCard |
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Title | Chair, Pharmaceutical Chemistry |
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Institution | UCSF School of Pharmacy |
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Department | Pharmaceutical Chemistry |
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Biography | 1979
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| 1981 | Damon M. Runyon-Walter Winchell Postdoctoral Fellowship | American Cancer Society | 1990 | | Pfizer Award for achievements in enzyme chemistry | Perdue University | 1991 | | Joseph Foster Award Lecturer | Washington State University | 1992 | | Alumni Achievement Award | Temple University | 1994 | | Distinguished Award Lecture | Rockefeller University | 1995 | | Stein Memorial Award Lecture | SUNY Stony Brook | 1997 | | Begelsteil Award Lecture | | 1997 | | Frontiers in Biological Sciences Lecture, Case Western Reserve | Washington State University | 1997 | | Distinguished Alumni Award | Protein Society | 1998 | | Christian B. Anfinsin Awar | American Peptide Society | 1998 | | Vincent du Vignead Award | NAS | 1999 | | Elected Member to the National Academy of Sciences | Protein Society | 2003 | | Hans Neurath Award | Harvard Medical School | 2005 | | Otto Krayer Lectureship | Boston College | 2005 | | Boston College Distinguished Lectureship in Chemistry | Stanford University | 2005 | | Cutting Award Lecture | | 2005 | | Braisted Award Lecture | American Cancer Society, Biotech | 2006 | | Perlman Lecture Award of the ACS Biotechnology Division | Nature Biotechnology | 2006 | | Shortlisted Nominee, Nature Biotechnology’s Who’s Who in Biotech, most significant contribution to b | CABM, Rutgers University | 2006 | | Paul Janssen Prize in Adv. Biotech and Medicine | Yale University | 2007 | | Fred Richards Distinguished Lecture | Rockefeller University | 2008 | | Jane Darnell Distinguished Lecture | Johns Hopkins University | 2009 | | Kossiakoff Lecture in Biophysics | University of Chicago | 2009 | | Herman S. Bloch Award, “for scientific excellence in industry” | Thomas Jefferson University | 2009 | | Weinhouse Lecture in Biochemistry | ASBMB | 2010 | | Merck Award | American Cancer Society | 2011 | | Smissman Award in Medicinal Chemistry | University of Illinois | 2011 | | Ada Doisy Lectures | Washington University, St Louis | 2011 | | Carl and Gerty Cori Lecture | University of Wisconsin, Madison | 2011 | | Green Lecture | St Luke's, Boise ID | 2011 | | Burkholder Award Lecture | Vanderbilt University, TN | 2012 | | Lubomir S. Hnilica Endowed Lecture | University of Washington, Seattle | 2012 | | Gordon Lecture | Gladstone, San Francisco | 2012 | | Keynote at Gladstone Scientific Retreat | UCSC Department of Chemistry and Biochemistry | 2013 | | Keynote Speaker | ICSB and Kyoto University | 2013 | | Keynote Speaker at ICSB Meeting | Tsinghua University | 2013 | | Keynote Speaker, Symposium of Frontier Sciences on New Drug Discovery | American Academy of Arts and Sciences | 2015 | | Elected Member of Academy |
Overview James "Jim" Wells focuses on understanding and modulating signaling processes in human cells through protein and small molecule design. His lab seeks activators as well as inhibitors of signaling molecules, including proteases and kinases, and uses them to study the consequences of triggering specific nodes of signaling circuits. He is especially interested in the inter-protein circuitry of pathways involved in cell death and inflammation, and the intra-protein allosteric circuitry that governs how distant functional sites in one protein communicate.
Research ORNG Applications Bibliographic
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications.
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Morgan CW, Diaz JE, Zeitlin SG, Gray DC, Wells J. Engineered cellular gene-replacement platform for selective and inducible proteolytic profiling. Proc Natl Acad Sci U S A. 2015 Jul 07; 112(27):8344-9. PMID: 26106156; PMCID: PMC4500269.
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Miersch S, Li Z, Hanna R, McLaughlin ME, Hornsby M, Matsuguchi T, Paduch M, Sääf A, Wells J, Koide S, Kossiakoff A, Sidhu SS. Scalable high throughput selection from phage-displayed synthetic antibody libraries. J Vis Exp. 2015 Jan 17; (95):51492. PMID: 25651360; PMCID: PMC4354533.
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Rettenmaier TJ, Sadowsky JD, Thomsen ND, Chen SC, Doak AK, Arkin MR, Wells J. A small-molecule mimic of a peptide docking motif inhibits the protein kinase PDK1. Proc Natl Acad Sci U S A. 2014 Dec 30; 111(52):18590-5. PMID: 25518860; PMCID: PMC4284534.
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Koerber JT, Hornsby MJ, Wells J. An improved single-chain Fab platform for efficient display and recombinant expression. J Mol Biol. 2015 Jan 30; 427(2):576-86. PMID: 25481745; PMCID: PMC4297586.
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Roberts TA, Norris FC, Carnaghan H, Savery D, Wells J, Siow B, Scambler PJ, Pierro A, De Coppi P, Eaton S, Lythgoe MF. In amnio MRI of mouse embryos. PLoS One. 2014; 9(10):e109143. PMID: 25330230; PMCID: PMC4198080.
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Ordureau A, Sarraf SA, Duda DM, Heo JM, Jedrychowski MP, Sviderskiy VO, Olszewski JL, Koerber JT, Xie T, Beausoleil SA, Wells J, Gygi SP, Schulman BA, Harper JW. Quantitative proteomics reveal a feedforward mechanism for mitochondrial PARKIN translocation and ubiquitin chain synthesis. Mol Cell. 2014 Nov 06; 56(3):360-75. PMID: 25284222; PMCID: PMC4254048.
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Julien O, Kampmann M, Bassik MC, Zorn JA, Venditto VJ, Shimbo K, Agard NJ, Shimada K, Rheingold AL, Stockwell BR, Weissman JS, Wells J. Unraveling the mechanism of cell death induced by chemical fibrils. Nat Chem Biol. 2014 Nov; 10(11):969-76. PMID: 25262416; PMCID: PMC4201873.
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Arkin MR, Tang Y, Wells J. Small-molecule inhibitors of protein-protein interactions: progressing toward the reality. Chem Biol. 2014 Sep 18; 21(9):1102-14. PMID: 25237857; PMCID: PMC4179228.
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Wells J, Kossiakoff AA. Cell biology. New tricks for an old dimer. Science. 2014 May 16; 344(6185):703-4. PMID: 24833381.
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Wiita AP, Hsu GW, Lu CM, Esensten JH, Wells J. Circulating proteolytic signatures of chemotherapy-induced cell death in humans discovered by N-terminal labeling. Proc Natl Acad Sci U S A. 2014 May 27; 111(21):7594-9. PMID: 24821784; PMCID: PMC4040605.
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Lodge JM, Rettenmaier TJ, Wells J, Pomerantz WC, Mapp AK. FP Tethering: a screening technique to rapidly identify compounds that disrupt protein-protein interactions. Medchemcomm. 2014 Mar 01; 5:370-375. PMID: 24795804.
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Ashkenazi A, Yuan J, Wells J. Regulated cell death part A: apoptotic mechanisms. Preface. Methods Enzymol. 2014; 544:xv. PMID: 24974300.
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Morgan CW, Julien O, Unger EK, Shah NM, Wells J. Turning on caspases with genetics and small molecules. Methods Enzymol. 2014; 544:179-213. PMID: 24974291; PMCID: PMC4249682.
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Wiita AP, Seaman JE, Wells J. Global analysis of cellular proteolysis by selective enzymatic labeling of protein N-termini. Methods Enzymol. 2014; 544:327-58. PMID: 24974296; PMCID: PMC4260929.
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Ashkenazi A, Wells J, Yuan J. Methods in Enzymology. Regulated cell death part B--necroptotic, autophagic and other non-apoptotic mechanisms. Preface. Methods Enzymol. 2014; 545:xiii. PMID: 25065895.
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Ostrem JM, Peters U, Sos ML, Wells J, Shokat KM. K-Ras(G12C) inhibitors allosterically control GTP affinity and effector interactions. Nature. 2013 Nov 28; 503(7477):548-51. PMID: 24256730; PMCID: PMC4274051.
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Koerber JT, Thomsen ND, Hannigan BT, Degrado WF, Wells J. Nature-inspired design of motif-specific antibody scaffolds. Nat Biotechnol. 2013 Oct; 31(10):916-21. PMID: 23955275; PMCID: PMC3795957.
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Carter PJ, Hazuda D, Wells J. Next generation therapeutics. Curr Opin Chem Biol. 2013 Jun; 17(3):317-9. PMID: 23683350.
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Thomsen ND, Koerber JT, Wells J. Structural snapshots reveal distinct mechanisms of procaspase-3 and -7 activation. Proc Natl Acad Sci U S A. 2013 May 21; 110(21):8477-82. PMID: 23650375; PMCID: PMC3666719.
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Wang N, Majmudar CY, Pomerantz WC, Gagnon JK, Sadowsky JD, Meagher JL, Johnson TK, Stuckey JA, Brooks CL, Wells J, Mapp AK. Ordering a dynamic protein via a small-molecule stabilizer. J Am Chem Soc. 2013 Mar 06; 135(9):3363-6. PMID: 23384013; PMCID: PMC3607081.
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Datta D, McClendon CL, Jacobson MP, Wells J. Substrate and inhibitor-induced dimerization and cooperativity in caspase-1 but not caspase-3. J Biol Chem. 2013 Apr 05; 288(14):9971-81. PMID: 23386603; PMCID: PMC3617296.
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Crawford ED, Seaman JE, Agard N, Hsu GW, Julien O, Mahrus S, Nguyen H, Shimbo K, Yoshihara HA, Zhuang M, Chalkley RJ, Wells J. The DegraBase: a database of proteolysis in healthy and apoptotic human cells. Mol Cell Proteomics. 2013 Mar; 12(3):813-24. PMID: 23264352; PMCID: PMC3591672.
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Zhuang M, Guan S, Wang H, Burlingame AL, Wells J. Substrates of IAP ubiquitin ligases identified with a designed orthogonal E3 ligase, the NEDDylator. Mol Cell. 2013 Jan 24; 49(2):273-82. PMID: 23201124; PMCID: PMC3557559.
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Crawford ED, Seaman JE, Barber AE, David DC, Babbitt PC, Burlingame AL, Wells J. Conservation of caspase substrates across metazoans suggests hierarchical importance of signaling pathways over specific targets and cleavage site motifs in apoptosis. Cell Death Differ. 2012 Dec; 19(12):2040-8. PMID: 22918439; PMCID: PMC3504717.
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Zorn JA, Wolan DW, Agard NJ, Wells J. Fibrils colocalize caspase-3 with procaspase-3 to foster maturation. J Biol Chem. 2012 Sep 28; 287(40):33781-95. PMID: 22872644; PMCID: PMC3460474.
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Shimbo K, Hsu GW, Nguyen H, Mahrus S, Trinidad JC, Burlingame AL, Wells J. Quantitative profiling of caspase-cleaved substrates reveals different drug-induced and cell-type patterns in apoptosis. Proc Natl Acad Sci U S A. 2012 Jul 31; 109(31):12432-7. PMID: 22802652; PMCID: PMC3412033.
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Agard NJ, Mahrus S, Trinidad JC, Lynn A, Burlingame AL, Wells J. Global kinetic analysis of proteolysis via quantitative targeted proteomics. Proc Natl Acad Sci U S A. 2012 Feb 07; 109(6):1913-8. PMID: 22308409; PMCID: PMC3277568.
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Gao J, Wells J. Identification of specific tethered inhibitors for caspase-5. Chem Biol Drug Des. 2012 Feb; 79(2):209-15. PMID: 22014003; PMCID: PMC3271850.
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Zorn JA, Wille H, Wolan DW, Wells J. Self-assembling small molecules form nanofibrils that bind procaspase-3 to promote activation. J Am Chem Soc. 2011 Dec 14; 133(49):19630-3. PMID: 22066605; PMCID: PMC3253528.
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Tang Y, Wells J, Arkin MR. Structural and enzymatic insights into caspase-2 protein substrate recognition and catalysis. J Biol Chem. 2011 Sep 30; 286(39):34147-54. PMID: 21828056; PMCID: PMC3190824.
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Sadowsky JD, Burlingame MA, Wolan DW, McClendon CL, Jacobson MP, Wells J. Turning a protein kinase on or off from a single allosteric site via disulfide trapping. Proc Natl Acad Sci U S A. 2011 Apr 12; 108(15):6056-61. PMID: 21430264; PMCID: PMC3076885.
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Crawford ED, Wells J. Caspase substrates and cellular remodeling. Annu Rev Biochem. 2011; 80:1055-87. PMID: 21456965.
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Gray DC, Mahrus S, Wells J. Activation of specific apoptotic caspases with an engineered small-molecule-activated protease. Cell. 2010 Aug 20; 142(4):637-46. PMID: 20723762; PMCID: PMC3689538.
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Barkan DT, Hostetter DR, Mahrus S, Pieper U, Wells J, Craik CS, Sali A. Prediction of protease substrates using sequence and structure features. Bioinformatics. 2010 Jul 15; 26(14):1714-22. PMID: 20505003; PMCID: PMC2894511.
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Zorn JA, Wells J. Turning enzymes ON with small molecules. Nat Chem Biol. 2010 Mar; 6(3):179-188. PMID: 20154666.
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Agard NJ, Maltby D, Wells J. Inflammatory stimuli regulate caspase substrate profiles. Mol Cell Proteomics. 2010 May; 9(5):880-93. PMID: 20173201; PMCID: PMC2871421.
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Wildes D, Wells J. Sampling the N-terminal proteome of human blood. Proc Natl Acad Sci U S A. 2010 Mar 09; 107(10):4561-6. PMID: 20173099; PMCID: PMC2842036.
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Pérez-Payá E, Orzáez M, Mondragón L, Wolan D, Wells J, Messeguer A, Vicent MJ. Molecules that modulate Apaf-1 activity. Med Res Rev. 2011 Jul; 31(4):649-75. PMID: 20099266.
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Brunger AT, Wells J. Warren L. DeLano 21 June 1972-3 November 2009. Nat Struct Mol Biol. 2009 Dec; 16(12):1202-3. PMID: 19956203.
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Wolan DW, Zorn JA, Gray DC, Wells J. Small-molecule activators of a proenzyme. Science. 2009 Nov 06; 326(5954):853-8. PMID: 19892984; PMCID: PMC2886848.
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Agard NJ, Wells J. Methods for the proteomic identification of protease substrates. Curr Opin Chem Biol. 2009 Dec; 13(5-6):503-9. PMID: 19729334; PMCID: PMC2787889.
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Hardy JA, Wells J. Dissecting an allosteric switch in caspase-7 using chemical and mutational probes. J Biol Chem. 2009 Sep 18; 284(38):26063-9. PMID: 19581639; PMCID: PMC2758006.
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Gao J, Sidhu SS, Wells J. Two-state selection of conformation-specific antibodies. Proc Natl Acad Sci U S A. 2009 Mar 03; 106(9):3071-6. PMID: 19208804; PMCID: PMC2651286.
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Mahrus S, Trinidad JC, Barkan DT, Sali A, Burlingame AL, Wells J. Global sequencing of proteolytic cleavage sites in apoptosis by specific labeling of protein N termini. Cell. 2008 Sep 05; 134(5):866-76. PMID: 18722006; PMCID: PMC2566540.
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Yoshihara HA, Mahrus S, Wells J. Tags for labeling protein N-termini with subtiligase for proteomics. Bioorg Med Chem Lett. 2008 Nov 15; 18(22):6000-3. PMID: 18762420; PMCID: PMC2590642.
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Datta D, Scheer JM, Romanowski MJ, Wells J. An allosteric circuit in caspase-1. J Mol Biol. 2008 Sep 19; 381(5):1157-67. PMID: 18590738; PMCID: PMC2626611.
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Wells J, McClendon CL. Reaching for high-hanging fruit in drug discovery at protein-protein interfaces. Nature. 2007 Dec 13; 450(7172):1001-9. PMID: 18075579.
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Tóth G, Mukhyala K, Wells J. Computational approach to site-directed ligand discovery. Proteins. 2007 Aug 01; 68(2):551-60. PMID: 17444517.
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Danis RP, Glassman AR, Aiello LP, Antoszyk AN, Beck RW, Browning DJ, Ciardella AP, Kinyoun JL, Murtha TJ, Topping TM, Shami M, Sharuk GS, Wells J. Diurnal variation in retinal thickening measurement by optical coherence tomography in center-involved diabetic macular edema. Arch Ophthalmol. 2006 Dec; 124(12):1701-7. PMID: 17159029; PMCID: PMC2279019.
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Thanos CD, DeLano WL, Wells J. Hot-spot mimicry of a cytokine receptor by a small molecule. Proc Natl Acad Sci U S A. 2006 Oct 17; 103(42):15422-7. PMID: 17032757; PMCID: PMC1592646.
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Matioc AA, Wells J. Positive pressure ventilation with the laryngeal mask airway in the operating room and prehospital: a practical review. J Trauma. 2006 Jun; 60(6):1371-6. PMID: 16766989.
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Scheer JM, Romanowski MJ, Wells J. A common allosteric site and mechanism in caspases. Proc Natl Acad Sci U S A. 2006 May 16; 103(20):7595-600. PMID: 16682620; PMCID: PMC1458511.
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Scheer JM, Wells J, Romanowski MJ. Malonate-assisted purification of human caspases. Protein Expr Purif. 2005 May; 41(1):148-53. PMID: 15802232.
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Buck E, Wells J. Disulfide trapping to localize small-molecule agonists and antagonists for a G protein-coupled receptor. Proc Natl Acad Sci U S A. 2005 Feb 22; 102(8):2719-24. PMID: 15710877; PMCID: PMC549436.
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Hardy JA, Wells J. Searching for new allosteric sites in enzymes. Curr Opin Struct Biol. 2004 Dec; 14(6):706-15. PMID: 15582395.
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Buck E, Bourne H, Wells J. Site-specific disulfide capture of agonist and antagonist peptides on the C5a receptor. J Biol Chem. 2005 Feb 11; 280(6):4009-12. PMID: 15550394.
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Hardy JA, Lam J, Nguyen JT, O'Brien T, Wells J. Discovery of an allosteric site in the caspases. Proc Natl Acad Sci U S A. 2004 Aug 24; 101(34):12461-6. PMID: 15314233; PMCID: PMC514654.
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Martin AG, Nguyen J, Wells J, Fearnhead HO. Apo cytochrome c inhibits caspases by preventing apoptosome formation. Biochem Biophys Res Commun. 2004 Jul 02; 319(3):944-50. PMID: 15184073.
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Stroud RM, Wells J. Mechanistic diversity of cytokine receptor signaling across cell membranes. Sci STKE. 2004 Apr 28; 2004(231):re7. PMID: 15126678.
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Arkin MR, Wells J. Small-molecule inhibitors of protein-protein interactions: progressing towards the dream. Nat Rev Drug Discov. 2004 Apr; 3(4):301-17. PMID: 15060526.
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Erlanson DA, Wells J, Braisted AC. Tethering: fragment-based drug discovery. Annu Rev Biophys Biomol Struct. 2004; 33:199-223. PMID: 15139811.
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Thanos CD, Randal M, Wells J. Potent small-molecule binding to a dynamic hot spot on IL-2. J Am Chem Soc. 2003 Dec 17; 125(50):15280-1. PMID: 14664558.
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Yaucher NE, Fish JT, Smith HW, Wells J. Propylene glycol-associated renal toxicity from lorazepam infusion. Pharmacotherapy. 2003 Sep; 23(9):1094-9. PMID: 14524641.
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Nguyen JT, Wells J. Direct activation of the apoptosis machinery as a mechanism to target cancer cells. Proc Natl Acad Sci U S A. 2003 Jun 24; 100(13):7533-8. PMID: 12808146; PMCID: PMC164621.
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Arkin MR, Randal M, DeLano WL, Hyde J, Luong TN, Oslob JD, Raphael DR, Taylor L, Wang J, McDowell RS, Wells J, Braisted AC. Binding of small molecules to an adaptive protein-protein interface. Proc Natl Acad Sci U S A. 2003 Feb 18; 100(4):1603-8. PMID: 12582206; PMCID: PMC149879.
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Wells J, Arkin M, Braisted A, DeLano W, McDowell B, Oslob J, Raimundo B, Randal M. Drug discovery at signaling interfaces. Ernst Schering Res Found Workshop. 2003; (42):19-27. PMID: 12664533.
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Johnson B, Gorman NT, Hird JF, Wadsworth DE, Wells J, Dyson SJ, Bleby J. BVA governance. Vet Rec. 2002 Aug 24; 151(8):247; author reply 247. PMID: 12219907.
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Chasens ER, Umlauf MG, Pillion DJ, Wells J. Nocturnal polyuria in type 2 diabetes: a symptom of obstructive sleep apnea. Diabetes Educ. 2002 May-Jun; 28(3):424-34. PMID: 12068651.
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Matioc AA, Wells J. The LMA-unique in a prehospital trauma patient: interaction with a semirigid cervical collar: a case report. J Trauma. 2002 Jan; 52(1):162-4. PMID: 11791069.
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Wells J, Wood KE. Acute barium poisoning treated with hemodialysis. Am J Emerg Med. 2001 Mar; 19(2):175-7. PMID: 11239275.
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Gerrard JA, Sparrow AD, Wells J. Metabolic databases - what next? Trends Biochem Sci. 2001 Feb; 26(2):137-40. PMID: 11166573.
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Erlanson DA, Braisted AC, Raphael DR, Randal M, Stroud RM, Gordon EM, Wells J. Site-directed ligand discovery. Proc Natl Acad Sci U S A. 2000 Aug 15; 97(17):9367-72. PMID: 10944209; PMCID: PMC16870.
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Weiss GA, Wells J, Sidhu SS. Mutational analysis of the major coat protein of M13 identifies residues that control protein display. Protein Sci. 2000 Apr; 9(4):647-54. PMID: 10794407; PMCID: PMC2144610.
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Sidhu SS, Weiss GA, Wells J. High copy display of large proteins on phage for functional selections. J Mol Biol. 2000 Feb 18; 296(2):487-95. PMID: 10669603.
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DeLano WL, Ultsch MH, de Vos AM, Wells J. Convergent solutions to binding at a protein-protein interface. Science. 2000 Feb 18; 287(5456):1279-83. PMID: 10678837.
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Sidhu SS, Lowman HB, Cunningham BC, Wells J. Phage display for selection of novel binding peptides. Methods Enzymol. 2000; 328:333-63. PMID: 11075354.
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Wells J, Ouslander JG, Meadows E, Johnson VY. Urinary incontinence in a geriatric patient with a complex neurologic history. J Wound Ostomy Continence Nurs. 1999 Sep; 26(5):270-4; discussion 274-5. PMID: 10795211.
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Atwell S, Wells J. Selection for improved subtiligases by phage display. Proc Natl Acad Sci U S A. 1999 Aug 17; 96(17):9497-502. PMID: 10449721; PMCID: PMC22237.
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Pearce KH, Cunningham BC, Fuh G, Teeri T, Wells J. Growth hormone binding affinity for its receptor surpasses the requirements for cellular activity. Biochemistry. 1999 Jan 05; 38(1):81-9. PMID: 9890885.
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Fairbrother WJ, Christinger HW, Cochran AG, Fuh G, Keenan CJ, Quan C, Shriver SK, Tom JY, Wells J, Cunningham BC. Novel peptides selected to bind vascular endothelial growth factor target the receptor-binding site. Biochemistry. 1998 Dec 22; 37(51):17754-64. PMID: 9922141.
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Arkin MR, Wells J. Probing the importance of second sphere residues in an esterolytic antibody by phage display. J Mol Biol. 1998 Dec 11; 284(4):1083-94. PMID: 9837728.
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Ballinger MD, Wells J. Will any dimer do? Nat Struct Biol. 1998 Nov; 5(11):938-40. PMID: 9808034.
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Ballinger MD, Jones JT, Lofgren JA, Fairbrother WJ, Akita RW, Sliwkowski MX, Wells J. Selection of heregulin variants having higher affinity for the ErbB3 receptor by monovalent phage display. J Biol Chem. 1998 May 08; 273(19):11675-84. PMID: 9565588.
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Jones JT, Ballinger MD, Pisacane PI, Lofgren JA, Fitzpatrick VD, Fairbrother WJ, Wells J, Sliwkowski MX. Binding interaction of the heregulinbeta egf domain with ErbB3 and ErbB4 receptors assessed by alanine scanning mutagenesis. J Biol Chem. 1998 May 08; 273(19):11667-74. PMID: 9565587.
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Fuh G, Li B, Crowley C, Cunningham B, Wells J. Requirements for binding and signaling of the kinase domain receptor for vascular endothelial growth factor. J Biol Chem. 1998 May 01; 273(18):11197-204. PMID: 9556609.
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Clackson T, Ultsch MH, Wells J, de Vos AM. Structural and functional analysis of the 1:1 growth hormone:receptor complex reveals the molecular basis for receptor affinity. J Mol Biol. 1998 Apr 17; 277(5):1111-28. PMID: 9571026.
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Wells J, Karr D. Interface pressure, wound healing, and satisfaction in the evaluation of a non-powered fluid mattress. Ostomy Wound Manage. 1998 Feb; 44(2):38-42, 44-6, 48 passim. PMID: 9526420.
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Ezra E, Wells J, Gray RH, Kinsella FM, Orr GM, Grego J, Arden GB, Gregor ZJ. Incidence of idiopathic full-thickness macular holes in fellow eyes. A 5-year prospective natural history study. Ophthalmology. 1998 Feb; 105(2):353-9. PMID: 9479299.
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Eckstein M, Wells J, Aylward B, Gregor Z. Surgical removal of non-age-related subfoveal choroidal neovascular membranes. Eye (Lond). 1998; 12 ( Pt 5):775-80. PMID: 10070507.
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Wiesmann C, Fuh G, Christinger HW, Eigenbrot C, Wells J, de Vos AM. Crystal structure at 1.7 A resolution of VEGF in complex with domain 2 of the Flt-1 receptor. Cell. 1997 Nov 28; 91(5):695-704. PMID: 9393862.
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Atwell S, Ultsch M, De Vos AM, Wells J. Structural plasticity in a remodeled protein-protein interface. Science. 1997 Nov 07; 278(5340):1125-8. PMID: 9353194.
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Baca M, Scanlan TS, Stephenson RC, Wells J. Phage display of a catalytic antibody to optimize affinity for transition-state analog binding. Proc Natl Acad Sci U S A. 1997 Sep 16; 94(19):10063-8. PMID: 9294163; PMCID: PMC23305.
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Starovasnik MA, Braisted AC, Wells J. Structural mimicry of a native protein by a minimized binding domain. Proc Natl Acad Sci U S A. 1997 Sep 16; 94(19):10080-5. PMID: 9294166; PMCID: PMC23311.
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Scheidig AJ, Hynes TR, Pelletier LA, Wells J, Kossiakoff AA. Crystal structures of bovine chymotrypsin and trypsin complexed to the inhibitor domain of Alzheimer's amyloid beta-protein precursor (APPI) and basic pancreatic trypsin inhibitor (BPTI): engineering of inhibitors with altered specificities. Protein Sci. 1997 Sep; 6(9):1806-24. PMID: 9300481; PMCID: PMC2143802.
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Pearce KH, Potts BJ, Presta LG, Bald LN, Fendly BM, Wells J. Mutational analysis of thrombopoietin for identification of receptor and neutralizing antibody sites. J Biol Chem. 1997 Aug 15; 272(33):20595-602. PMID: 9252374.
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Cunningham BC, Wells J. Minimized proteins. Curr Opin Struct Biol. 1997 Aug; 7(4):457-62. PMID: 9266165.
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Kroodsma DE, Houlihan PW, Fallon PA, Wells J. Song development by grey catbirds Anim Behav. 1997 Aug; 54(2):457-64. PMID: 9268478.
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Muller YA, Li B, Christinger HW, Wells J, Cunningham BC, de Vos AM. Vascular endothelial growth factor: crystal structure and functional mapping of the kinase domain receptor binding site. Proc Natl Acad Sci U S A. 1997 Jul 08; 94(14):7192-7. PMID: 9207067; PMCID: PMC23789.
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Atwell S, Ridgway JB, Wells J, Carter P. Stable heterodimers from remodeling the domain interface of a homodimer using a phage display library. J Mol Biol. 1997 Jul 04; 270(1):26-35. PMID: 9231898.
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Baca M, Presta LG, O'Connor SJ, Wells J. Antibody humanization using monovalent phage display. J Biol Chem. 1997 Apr 18; 272(16):10678-84. PMID: 9099717.
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Braisted AC, Judice JK, Wells J. Synthesis of proteins by subtiligase. Methods Enzymol. 1997; 289:298-313. PMID: 9353727.
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Ballinger MD, Tom J, Wells J. Furilisin: a variant of subtilisin BPN' engineered for cleaving tribasic substrates. Biochemistry. 1996 Oct 22; 35(42):13579-85. PMID: 8885837.
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Clark R, Olson K, Fuh G, Marian M, Mortensen D, Teshima G, Chang S, Chu H, Mukku V, Canova-Davis E, Somers T, Cronin M, Winkler M, Wells J. Long-acting growth hormones produced by conjugation with polyethylene glycol. J Biol Chem. 1996 Sep 06; 271(36):21969-77. PMID: 8703002.
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Pearce KH, Ultsch MH, Kelley RF, de Vos AM, Wells J. Structural and mutational analysis of affinity-inert contact residues at the growth hormone-receptor interface. Biochemistry. 1996 Aug 13; 35(32):10300-7. PMID: 8756685.
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Wells J. Hormone mimicry. Science. 1996 Jul 26; 273(5274):449-50. PMID: 8677438.
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Braisted AC, Wells J. Minimizing a binding domain from protein A. Proc Natl Acad Sci U S A. 1996 Jun 11; 93(12):5688-92. PMID: 8650153; PMCID: PMC39121.
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Wells J, Murthy R, Chibber R, Nunn A, Molinatti PA, Kohner EM, Gregor ZJ. Levels of vascular endothelial growth factor are elevated in the vitreous of patients with subretinal neovascularisation. Br J Ophthalmol. 1996 Apr; 80(4):363-6. PMID: 8703891; PMCID: PMC505466.
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Wells J. Binding in the growth hormone receptor complex. Proc Natl Acad Sci U S A. 1996 Jan 09; 93(1):1-6. PMID: 8552582; PMCID: PMC40168.
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Wells J, Gregor ZJ. Surgical treatment of full-thickness macular holes using autologous serum. Eye (Lond). 1996; 10 ( Pt 5):593-9. PMID: 8977788.
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Wells J, de Vos AM. Hematopoietic receptor complexes. Annu Rev Biochem. 1996; 65:609-34. PMID: 8811191.
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Li B, Tom JY, Oare D, Yen R, Fairbrother WJ, Wells J, Cunningham BC. Minimization of a polypeptide hormone. Science. 1995 Dec 08; 270(5242):1657-60. PMID: 7502074.
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Goddard AD, Covello R, Luoh SM, Clackson T, Attie KM, Gesundheit N, Rundle AC, Wells J, Carlsson LM. Mutations of the growth hormone receptor in children with idiopathic short stature. The Growth Hormone Insensitivity Study Group. N Engl J Med. 1995 Oct 26; 333(17):1093-8. PMID: 7565946.
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Ballinger MD, Tom J, Wells J. Designing subtilisin BPN' to cleave substrates containing dibasic residues. Biochemistry. 1995 Oct 17; 34(41):13312-9. PMID: 7577915.
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Wells J. Structural and functional epitopes in the growth hormone receptor complex. Biotechnology (N Y). 1995 Jul; 13(7):647-51. PMID: 9634801.
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Fuh G, Wells J. Prolactin receptor antagonists that inhibit the growth of breast cancer cell lines. J Biol Chem. 1995 Jun 02; 270(22):13133-7. PMID: 7768908.
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Sell RL, Wells J, Wypij D. The prevalence of homosexual behavior and attraction in the United States, the United Kingdom and France: results of national population-based samples. Arch Sex Behav. 1995 Jun; 24(3):235-48. PMID: 7611844.
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Clackson T, Wells J. A hot spot of binding energy in a hormone-receptor interface. Science. 1995 Jan 20; 267(5196):383-6. PMID: 7529940.
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Chang TK, Jackson DY, Burnier JP, Wells J. Subtiligase: a tool for semisynthesis of proteins. Proc Natl Acad Sci U S A. 1994 Dec 20; 91(26):12544-8. PMID: 7809074; PMCID: PMC45475.
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Jin L, Wells J. Dissecting the energetics of an antibody-antigen interface by alanine shaving and molecular grafting. Protein Sci. 1994 Dec; 3(12):2351-7. PMID: 7538848; PMCID: PMC2142782.
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Wells J. Readability of HIV/AIDS educational materials: the role of the medium of communication, target audience, and producer characteristics. Patient Educ Couns. 1994 Dec; 24(3):249-59. PMID: 7753718.
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Wroblewski JJ, Wells J, Eckstein A, Fitzke FW, Jubb C, Keen TJ, Inglehearn CF, Bhattacharya SS, Arden GB, Jay MR, et al. Ocular findings associated with a 3 base pair deletion in the peripherin-RDS gene in autosomal dominant retinitis pigmentosa. Br J Ophthalmol. 1994 Nov; 78(11):831-6. PMID: 7848979; PMCID: PMC504967.
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Jackson DY, Burnier J, Quan C, Stanley M, Tom J, Wells J. A designed peptide ligase for total synthesis of ribonuclease A with unnatural catalytic residues. Science. 1994 Oct 14; 266(5183):243-7. PMID: 7939659.
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Matthews DJ, Wells J. Engineering an interfacial zinc site to increase hormone-receptor affinity. Chem Biol. 1994 Sep; 1(1):25-30. PMID: 9383367.
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Holz FG, Wolfensberger TJ, Piguet B, Gross-Jendroska M, Wells J, Minassian DC, Chisholm IH, Bird AC. Bilateral macular drusen in age-related macular degeneration. Prognosis and risk factors. Ophthalmology. 1994 Sep; 101(9):1522-8. PMID: 8090455.
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Matthews DJ, Goodman LJ, Gorman CM, Wells J. A survey of furin substrate specificity using substrate phage display. Protein Sci. 1994 Aug; 3(8):1197-205. PMID: 7987214; PMCID: PMC2142928.
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Wells J, Doughty DB. Pouching principles and products. Ostomy Wound Manage. 1994 Jul-Aug; 40(6):50-8, 60-3. PMID: 7880372.
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Ilondo MM, Damholt AB, Cunningham BA, Wells J, De Meyts P, Shymko RM. Receptor dimerization determines the effects of growth hormone in primary rat adipocytes and cultured human IM-9 lymphocytes. Endocrinology. 1994 Jun; 134(6):2397-403. PMID: 8194466.
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Cunningham BC, Lowe DG, Li B, Bennett BD, Wells J. Production of an atrial natriuretic peptide variant that is specific for type A receptor. EMBO J. 1994 Jun 01; 13(11):2508-15. PMID: 8013451; PMCID: PMC395124.
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Jamieson AC, Kim SH, Wells J. In vitro selection of zinc fingers with altered DNA-binding specificity. Biochemistry. 1994 May 17; 33(19):5689-95. PMID: 8180194.
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Wells J, Fairbrother WJ, Otlewski J, Laskowski M, Burnier J. A reinvestigation of a synthetic peptide (TrPepz) designed to mimic trypsin. Proc Natl Acad Sci U S A. 1994 May 10; 91(10):4110-4. PMID: 8183880; PMCID: PMC43734.
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Clackson T, Wells J. In vitro selection from protein and peptide libraries. Trends Biotechnol. 1994 May; 12(5):173-84. PMID: 7764900.
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Baumann G, Lowman HB, Mercado M, Wells J. The stoichiometry of growth hormone-binding protein complexes in human plasma: comparison with cell surface receptors. J Clin Endocrinol Metab. 1994 May; 78(5):1113-8. PMID: 8175967.
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Whittemore R, Wells J, Castellsague X. A second-generation study of 427 probands with congenital heart defects and their 837 children. J Am Coll Cardiol. 1994 May; 23(6):1459-67. PMID: 8176107.
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Wells J, Ruscavage D, Parker B, McArthur L. Literacy of women attending family planning clinics in Virginia and reading levels of brochures on HIV prevention. Fam Plann Perspect. 1994 May-Jun; 26(3):113-5, 131. PMID: 8070547.
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Wells J, Ellerine NP, Fernhoff PM, Waring GO. Corneal opacities in spondyloepiphyseal dysplasia tarda. Cornea. 1994 May; 13(3):280-3. PMID: 8033583.
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Wells J. Structural and functional basis for hormone binding and receptor oligomerization. Curr Opin Cell Biol. 1994 Apr; 6(2):163-73. PMID: 8024806.
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Jin L, Cohen FE, Wells J. Structure from function: screening structural models with functional data. Proc Natl Acad Sci U S A. 1994 Jan 04; 91(1):113-7. PMID: 7506411; PMCID: PMC42896.
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Wroblewski JJ, Wells J, Eckstein A, Fitzke F, Jubb C, Keen TJ, Inglehearn C, Bhattacharya S, Arden GB, Jay M, et al. Macular dystrophy associated with mutations at codon 172 in the human retinal degeneration slow gene. Ophthalmology. 1994 Jan; 101(1):12-22. PMID: 8302543.
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Lowman HB, Wells J. Affinity maturation of human growth hormone by monovalent phage display. J Mol Biol. 1993 Dec 05; 234(3):564-78. PMID: 8254660.
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Cunningham BC, Wells J. Comparison of a structural and a functional epitope. J Mol Biol. 1993 Dec 05; 234(3):554-63. PMID: 7504735.
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Feldman M, Ruan W, Cunningham BC, Wells J, Kleinberg DL. Evidence that the growth hormone receptor mediates differentiation and development of the mammary gland. Endocrinology. 1993 Oct; 133(4):1602-8. PMID: 8404600.
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Piguet B, Wells J, Palmvang IB, Wormald R, Chisholm IH, Bird AC. Age-related Bruch's membrane change: a clinical study of the relative role of heredity and environment. Br J Ophthalmol. 1993 Jul; 77(7):400-3. PMID: 8343465; PMCID: PMC504547.
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Matthews DJ, Wells J. Substrate phage: selection of protease substrates by monovalent phage display. Science. 1993 May 21; 260(5111):1113-7. PMID: 8493554.
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Fuh G, Colosi P, Wood WI, Wells J. Mechanism-based design of prolactin receptor antagonists. J Biol Chem. 1993 Mar 15; 268(8):5376-81. PMID: 8449901.
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Wells J, Cunningham BC, Fuh G, Lowman HB, Bass SH, Mulkerrin MG, Ultsch M, deVos AM. The molecular basis for growth hormone-receptor interactions. Recent Prog Horm Res. 1993; 48:253-75. PMID: 8441850.
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Wells J, de Vos AM. Structure and function of human growth hormone: implications for the hematopoietins. Annu Rev Biophys Biomol Struct. 1993; 22:329-51. PMID: 7688610.
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Retchin SM, Wells J, Valleron AJ, Albrecht GL. Health behavior changes in the United States, the United Kingdom, and France. J Gen Intern Med. 1992 Nov-Dec; 7(6):615-22. PMID: 1453245.
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Weber JR, Nelson C, Cunningham BC, Wells J, Fong S. Immunodominant structures of human growth hormone identified by homolog-scanning mutagenesis. Mol Immunol. 1992 Sep; 29(9):1081-8. PMID: 1495498.
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Braxton S, Wells J. Incorporation of a stabilizing Ca(2+)-binding loop into subtilisin BPN'. Biochemistry. 1992 Sep 01; 31(34):7796-801. PMID: 1510966.
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Jin L, Fendly BM, Wells J. High resolution functional analysis of antibody-antigen interactions. J Mol Biol. 1992 Aug 05; 226(3):851-65. PMID: 1380563.
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Wells J, Lowman HB. Rapid evolution of peptide and protein binding properties in vitro. Curr Opin Biotechnol. 1992 Aug; 3(4):355-62. PMID: 1368436.
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Kern M, Dolan PL, Mazzeo RS, Wells J, Dohm GL. Effect of aging and exercise on GLUT-4 glucose transporters in muscle. Am J Physiol. 1992 Aug; 263(2 Pt 1):E362-7. PMID: 1514619.
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Laurier D, Guiguet M, Chau NP, Wells J, Valleron AJ. Prevalence of obesity: a comparative survey in France, the United Kingdom and the United States. Int J Obes Relat Metab Disord. 1992 Aug; 16(8):565-72. PMID: 1326486.
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Fuh G, Cunningham BC, Fukunaga R, Nagata S, Goeddel DV, Wells J. Rational design of potent antagonists to the human growth hormone receptor. Science. 1992 Jun 19; 256(5064):1677-80. PMID: 1535167.
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Fu YK, Arkins S, Fuh G, Cunningham BC, Wells J, Fong S, Cronin MJ, Dantzer R, Kelley KW. Growth hormone augments superoxide anion secretion of human neutrophils by binding to the prolactin receptor. J Clin Invest. 1992 Feb; 89(2):451-7. PMID: 1310696; PMCID: PMC442872.
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Laurier D, Guiguet M, Chau NP, Wells J, Valleron AJ. [Prevalence of obesity in France]. Rev Epidemiol Sante Publique. 1992; 40(5):365-7. PMID: 1480813.
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Bechara SJ, Grossniklaus HE, Waring GO, Wells J. Keratoconus associated with posterior polymorphous dystrophy. Am J Ophthalmol. 1991 Dec 15; 112(6):729-31. PMID: 1957913.
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Lowman HB, Bass SH, Simpson N, Wells J. Selecting high-affinity binding proteins by monovalent phage display. Biochemistry. 1991 Nov 12; 30(45):10832-8. PMID: 1932005.
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Cunningham BC, Ultsch M, De Vos AM, Mulkerrin MG, Clauser KR, Wells J. Dimerization of the extracellular domain of the human growth hormone receptor by a single hormone molecule. Science. 1991 Nov 08; 254(5033):821-5. PMID: 1948064.
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Cunningham BC, Mulkerrin MG, Wells J. Dimerization of human growth hormone by zinc. Science. 1991 Aug 02; 253(5019):545-8. PMID: 1907025.
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Guiguet M, Cohen M, Flahault A, Wells J, Valleron AJ. French intravenous drug users: knowledge and sexual behavior change. Eur J Epidemiol. 1991 Jul; 7(4):423-6. PMID: 1915793.
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Braxton S, Wells J. The importance of a distal hydrogen bonding group in stabilizing the transition state in subtilisin BPN'. J Biol Chem. 1991 Jun 25; 266(18):11797-800. PMID: 1904870.
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Carter P, Abrahmsén L, Wells J. Probing the mechanism and improving the rate of substrate-assisted catalysis in subtilisin BPN'. Biochemistry. 1991 Jun 25; 30(25):6142-8. PMID: 2059622.
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Lowman HB, Cunningham BC, Wells J. Mutational analysis and protein engineering of receptor-binding determinants in human placental lactogen. J Biol Chem. 1991 Jun 15; 266(17):10982-8. PMID: 2040614.
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Bass SH, Mulkerrin MG, Wells J. A systematic mutational analysis of hormone-binding determinants in the human growth hormone receptor. Proc Natl Acad Sci U S A. 1991 May 15; 88(10):4498-502. PMID: 2034689; PMCID: PMC51688.
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Abrahmsén L, Tom J, Burnier J, Butcher KA, Kossiakoff A, Wells J. Engineering subtilisin and its substrates for efficient ligation of peptide bonds in aqueous solution. Biochemistry. 1991 Apr 30; 30(17):4151-9. PMID: 2021606.
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Cunningham BC, Wells J. Rational design of receptor-specific variants of human growth hormone. Proc Natl Acad Sci U S A. 1991 Apr 15; 88(8):3407-11. PMID: 2014261; PMCID: PMC51456.
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Wells J. Systematic mutational analyses of protein-protein interfaces. Methods Enzymol. 1991; 202:390-411. PMID: 1723781.
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Cunningham BC, Bass S, Fuh G, Wells J. Zinc mediation of the binding of human growth hormone to the human prolactin receptor. Science. 1990 Dec 21; 250(4988):1709-12. PMID: 2270485.
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Wells J. Additivity of mutational effects in proteins. Biochemistry. 1990 Sep 18; 29(37):8509-17. PMID: 2271534.
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Kern M, Wells J, Stephens JM, Elton CW, Friedman JE, Tapscott EB, Pekala PH, Dohm GL. Insulin responsiveness in skeletal muscle is determined by glucose transporter (Glut4) protein level. Biochem J. 1990 Sep 01; 270(2):397-400. PMID: 2205203; PMCID: PMC1131735.
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Casla A, Rovira A, Wells J, Dohm GL. Increased glucose transporter (GLUT4) protein expression in hyperthyroidism. Biochem Biophys Res Commun. 1990 Aug 31; 171(1):182-8. PMID: 2203344.
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Ellerby LM, Escobar WA, Fink AL, Mitchinson C, Wells J. The role of lysine-234 in beta-lactamase catalysis probed by site-directed mutagenesis. Biochemistry. 1990 Jun 19; 29(24):5797-806. PMID: 1974463.
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Cunningham BC, Henner DJ, Wells J. Engineering human prolactin to bind to the human growth hormone receptor. Science. 1990 Mar 23; 247(4949 Pt 1):1461-5. PMID: 2321008.
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Fuh G, Mulkerrin MG, Bass S, McFarland N, Brochier M, Bourell JH, Light DR, Wells J. The human growth hormone receptor. Secretion from Escherichia coli and disulfide bonding pattern of the extracellular binding domain. J Biol Chem. 1990 Feb 25; 265(6):3111-5. PMID: 2406245.
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Contreras I, Dohm GL, Abdallah S, Wells J, Mooney N, Rovira A, Caro JF. The effect of fasting on the activation in vivo of the insulin receptor kinase. Biochem J. 1990 Feb 01; 265(3):887-90. PMID: 2407238; PMCID: PMC1133714.
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Kesten S, Maleki-Yazdi R, Sanders BR, Wells J, McKillop SL, Chapman KR, Rebuck AS. Respiratory rate during acute asthma. Chest. 1990 Jan; 97(1):58-62. PMID: 2403901.
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Zonana HV, Bartel RL, Wells J, Buchanan JA, Getz MA. Part II: Sex differences in persons found not guilty by reason of insanity: analysis of data from the Connecticut NGRI Registry. Bull Am Acad Psychiatry Law. 1990; 18(2):129-42. PMID: 2372574.
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Zonana HV, Wells J, Getz MA, Buchanan J. Part I: The NGRI Registry: initial analyses of data collected on Connecticut insanity acquittees. Bull Am Acad Psychiatry Law. 1990; 18(2):115-28. PMID: 2372573.
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Bass S, Greene R, Wells J. Hormone phage: an enrichment method for variant proteins with altered binding properties. Proteins. 1990; 8(4):309-14. PMID: 1708882.
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Carter P, Wells J. Functional interaction among catalytic residues in subtilisin BPN'. Proteins. 1990; 7(4):335-42. PMID: 2199971.
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Wells J, Cunningham BC. Identification and design of binding determinants in proteins. Biochem Soc Symp. 1990; 57:143-5. PMID: 2099738.
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Cunningham BC, Wells J. High-resolution epitope mapping of hGH-receptor interactions by alanine-scanning mutagenesis. Science. 1989 Jun 02; 244(4908):1081-5. PMID: 2471267.
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Mitchinson C, Wells J. Protein engineering of disulfide bonds in subtilisin BPN'. Biochemistry. 1989 May 30; 28(11):4807-15. PMID: 2504281.
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Fushiki T, Wells J, Tapscott EB, Dohm GL. Changes in glucose transporters in muscle in response to exercise. Am J Physiol. 1989 May; 256(5 Pt 1):E580-7. PMID: 2655468.
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Cunningham BC, Jhurani P, Ng P, Wells J. Receptor and antibody epitopes in human growth hormone identified by homolog-scanning mutagenesis. Science. 1989 Mar 10; 243(4896):1330-6. PMID: 2466339.
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Carter P, Nilsson B, Burnier JP, Burdick D, Wells J. Engineering subtilisin BPN' for site-specific proteolysis. Proteins. 1989; 6(3):240-8. PMID: 2516317.
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Dunlop BD, Wells J, Wilensky GR. The influence of source of insurance coverage on the health care utilization patterns of the elderly. J Health Hum Resour Adm. 1989; 11(3):285-311. PMID: 10303344.
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Wells J, Estell DA. Subtilisin--an enzyme designed to be engineered. Trends Biochem Sci. 1988 Aug; 13(8):291-7. PMID: 3154281.
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Carter P, Wells J. Dissecting the catalytic triad of a serine protease. Nature. 1988 Apr 07; 332(6164):564-8. PMID: 3282170.
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Wells J, Zuercher A, Clinton J. GrantWatch. Foundation funding for AIDS education. Health Aff (Millwood). 1988; 7(5):146-58. PMID: 3229713.
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Wells J, Cunningham BC, Graycar TP, Estell DA. Recruitment of substrate-specificity properties from one enzyme into a related one by protein engineering. Proc Natl Acad Sci U S A. 1987 Aug; 84(15):5167-71. PMID: 3299378; PMCID: PMC298815.
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Cunningham BC, Wells J. Improvement in the alkaline stability of subtilisin using an efficient random mutagenesis and screening procedure. Protein Eng. 1987 Aug-Sep; 1(4):319-25. PMID: 3334089.
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Carter P, Wells J. Engineering enzyme specificity by "substrate-assisted catalysis". Science. 1987 Jul 24; 237(4813):394-9. PMID: 3299704.
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Pearson HA, Guiliotis DK, Rink L, Wells J. Patient age distribution in thalassemia major: changes from 1973 to 1985. Pediatrics. 1987 Jul; 80(1):53-7. PMID: 3601518.
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Wells J, Powers DB, Bott RR, Graycar TP, Estell DA. Designing substrate specificity by protein engineering of electrostatic interactions. Proc Natl Acad Sci U S A. 1987 Mar; 84(5):1219-23. PMID: 3547407; PMCID: PMC304398.
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Wells J, Cunningham BC, Graycar TP, Estell DA, Carter P. On the evolution of specificity and catalysis in subtilisin. Cold Spring Harb Symp Quant Biol. 1987; 52:647-52. PMID: 3331349.
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Wells J. Foundation funding for AIDS programs. Health Aff (Millwood). 1987; 6(3):113-23. PMID: 3679069.
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Wells J, Smyth RJ, Rebuck AS. Thoracoabdominal motion in response to treadmill and cycle exercise. Am Rev Respir Dis. 1986 Dec; 134(6):1125-8. PMID: 3098144.
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Estell DA, Graycar TP, Miller JV, Powers DB, Wells J, Burnier JP, Ng PG. Probing steric and hydrophobic effects on enzyme-substrate interactions by protein engineering. Science. 1986 Aug 08; 233(4764):659-63. PMID: 17835820.
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Wells J, Powers DB. In vivo formation and stability of engineered disulfide bonds in subtilisin. J Biol Chem. 1986 May 15; 261(14):6564-70. PMID: 3516996.
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Power SD, Adams RM, Wells J. Secretion and autoproteolytic maturation of subtilisin. Proc Natl Acad Sci U S A. 1986 May; 83(10):3096-100. PMID: 3517850; PMCID: PMC323459.
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Nakamaye KL, Wells J, Bridenbaugh RL, Okamoto Y, Yount RG. 2-[(4-Azido-2-nitrophenyl)amino]ethyl triphosphate, a novel chromophoric and photoaffinity analogue of ATP. Synthesis, characterization, and interaction with myosin subfragment 1. Biochemistry. 1985 Sep 10; 24(19):5226-35. PMID: 4074691.
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Estell DA, Graycar TP, Wells J. Engineering an enzyme by site-directed mutagenesis to be resistant to chemical oxidation. J Biol Chem. 1985 Jun 10; 260(11):6518-21. PMID: 3922976.
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Wells J, Vasser M, Powers DB. Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites. Gene. 1985; 34(2-3):315-23. PMID: 3891521.
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Perkins WJ, Wells J, Yount RG. Characterization of the properties of ethenoadenosine nucleotides bound or trapped at the active site of myosin subfragment 1. Biochemistry. 1984 Aug 14; 23(17):3994-4002. PMID: 6237680.
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Wells J, Swyryd EA, Stark GR. An improved method for purifying 2',5'-oligoadenylate synthetases. J Biol Chem. 1984 Jan 25; 259(2):1363-70. PMID: 6693390.
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Griffith MJ, Wells J. Awareness by consumers and the adoption of a new service: an HMO example. J Health Care Mark. 1984; 4(1):17-24. PMID: 10266249.
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Wells J, Ferrari E, Henner DJ, Estell DA, Chen EY. Cloning, sequencing, and secretion of Bacillus amyloliquefaciens subtilisin in Bacillus subtilis. Nucleic Acids Res. 1983 Nov 25; 11(22):7911-25. PMID: 6316278; PMCID: PMC326549.
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Wells J, Zipp JF, Schuette PT, McEleney J. Musculoskeletal disorders among letter carriers. A comparison of weight carrying, walking & sedentary occupations. J Occup Med. 1983 Nov; 25(11):814-20. PMID: 6644392.
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Dalbey RE, Wells J, Yount RG. Trapping of transition metal-nucleotide complexes in myosin subfragment 1 by cross-linking thiols; divalent transition metal probes of the active site. Biochemistry. 1983 Jan 18; 22(2):490-6. PMID: 6824640.
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Wells J, Strickland DE. Physiogenic bias as invalidity in psychiatric symptom scales. J Health Soc Behav. 1982 Sep; 23(3):235-52. PMID: 7175160.
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Wells J, Yount RG. Chemical modification of myosin by active-site trapping of metal-nucleotides with thiol crosslinking reagents. Methods Enzymol. 1982; 85 Pt B:93-116. PMID: 7121292.
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Wells J, Knoeber C, Sheldon MC, Werber MM, Yount RG. Cross-linking of myosin subfragment 1. Nucleotide-enhanced modification by a variety of bifunctional reagents. J Biol Chem. 1980 Dec 10; 255(23):11135-40. PMID: 7440533.
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Wells J, Werber MM, Yount RG. Mechanism of inactivation of myosin subfragment 1 by Co(III)phenATP. A reinvestigation. J Biol Chem. 1980 Aug 25; 255(16):7552-5. PMID: 6447149.
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Wells J, Yount RG. Reaction of 5,5'-dithiobis(2-nitrobenzoic acid) with myosin subfragment one: evidence for formation of a single protein disulfide with trapping of metal nucleotide at the active site. Biochemistry. 1980 Apr 15; 19(8):1711-7. PMID: 6445748.
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Wells J, Sheldon M, Yount RG. Magnesium nucleotide is stoichiometrically trapped at the active site of myosin and its active proteolytic fragments by thiol cross-linking reagents. J Biol Chem. 1980 Feb 25; 255(4):1598-602. PMID: 6444414.
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Wells, J.A. and Yount, R.G. . "Reaction of 5,5'-dithiobis (2-nitrobenzoic acid) with myosin subfragment one: Evidence for formation of a single protein disulfide with trapping of metal nucleotide at the active site". Biochemistry. 1980; 19:1711-1717.
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Wells, J.A., Sheldon, M., and Yount, R.G. "Magnesium nucleotide is stoichiometrically trapped at the active site of myosin and its proteolytic subfragments by thiol cross-linking reagents". J. Biol. Chem. 1980; 255:1598-1602.
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Wells J, Werber MM, Yount RG. Inactivation of myosin subfragment one by cobalt(II)/cobalt(III) phenanthroline complexes. 2. Cobalt chelation of two critical SH groups. Biochemistry. 1979 Oct 30; 18(22):4800-5. PMID: 159719.
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Wells J, Werber MM, Legg JI, Yount RG. Inactivation of myosin subfragment one by cobalt(II)/cobalt(III) phenanthroline complexes. I. Incorporation of Co(III) by in situ oxidation of Co(II). Biochemistry. 1979 Oct 30; 18(22):4793-9. PMID: 41570.
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Wells J, Yount RG. Active site trapping of nucleotides by crosslinking two sulfhydryls in myosin subfragment 1. Proc Natl Acad Sci U S A. 1979 Oct; 76(10):4966-70. PMID: 159451; PMCID: PMC413059.
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House JS, McMichael AJ, Wells J, Kaplan BH, Landerman LR. Occupational stress and health among factory workers. J Health Soc Behav. 1979 Jun; 20(2):139-60. PMID: 479526.
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Wells, J.A., Werber, M.M. and Yount, R.G. . "Inactivation of myosin subfragment one by cobalt (II)/cobalt (III) phenanthroline complexes. 1. Incorporation of Co(III) by in situ oxidation of Co(II)". Biochemistry. 1979; 18:4793-4799. .
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Wells, J.A., Werber, M.M., Legg, J.I., and Yount, R.G. . "Inactivation of myosin subfragment one by cobalt (II)/cobalt (III) phenanthroline complexes. 2. Cobalt chelation of two critical SH groups". Biochemistry. 1979; 18:4800-4805.
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Wells, J.A. and Yount, R.G. . "Active site trapping of nucleotides by crosslinking two sulfhydryls in myosin sub-fragment 1". Proc. Natl. Acad. Sci. USA. 1979; 76:4966-4970.
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Fisher JC, Wells J, Fulwider BT, Edgerton MT. Do we need a burn severity grading system. J Trauma. 1977 Mar; 17(3):252-5. PMID: 850285.
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Wells J. Issues in specialty certification. ANA Publ. 1975; (PMH-2):6-14. PMID: 1045880.
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1980 | 6 | 1982 | 2 | 1983 | 3 | 1984 | 3 | 1985 | 3 | 1986 | 4 | 1987 | 7 | 1988 | 3 | 1989 | 6 | 1990 | 14 | 1991 | 12 | 1992 | 10 | 1993 | 8 | 1994 | 21 | 1995 | 7 | 1996 | 9 | 1997 | 12 | 1998 | 10 | 1999 | 3 | 2000 | 5 | 2001 | 2 | 2002 | 3 | 2003 | 5 | 2004 | 7 | 2005 | 2 | 2006 | 4 | 2007 | 2 | 2008 | 3 | 2009 | 5 | 2010 | 6 | 2011 | 5 | 2012 | 6 | 2013 | 7 | 2014 | 13 | 2015 | 2 |
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