Bo Huang, PhD

Title(s)Professor, Pharmaceutical Chemistry
SchoolSchool of Pharmacy
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    Collapse Biography 
    Collapse Awards and Honors
    2017UCSF Byers Award for Basic Science
    2016American Society for Cell Biology Young Life Scientist Award
    2011NIH Director's New Innovator Award
    2010Searle Scholar
    2010Packard Fellowship for Science and Engineering
    2007GE & Science Young Life Scientist Award, North America Regional Winner
    2001  - 2004Stanford Graduate Fellowship
    2006Annual Reviews Prize in Physical Chemistry, Stanford University

    Collapse Overview 
    Collapse Overview
    Cellular processes are carried out by coordinated participation of many biomolecules in a tiny volume. Many people have been dreaming to see clear pictures of these processes in order to understand how these molecules work together. Taking on this challenge, we are developing new visualization techniques and imaging probes by combining super-resolution microscopy, protein engineering and microfluidic automation. We are particularly interested in the following problems:

    (1) Physical organization and dynamics of the genome,
    (2) Architecture of large protein complexes such as the centrosome, and
    (3) Spatial distribution of membrane proteins, particularly G-protein coupled receptors and neuron adhesion molecules, and how this distribution defines their signaling specificity.

    In order to study these systems, we are developing the following microscopy technologies:

    (1) Super-resolution and light-sheet microscopes that can visualize subcellular structures at a higher spatial resolution, record long term cell behavior, and track cells in intact animals, and
    (2) New fluorescent probes based on fluorescent proteins, nanobodies and aptamers so that biological questions can be converted into "imageable" ones.

    Collapse Research 
    Collapse Research Activities and Funding
    Rapid screening of gene-edited cells
    NIH/NIGMS R21GM129652Aug 1, 2018 - Jun 30, 2020
    Role: Principal Investigator
    Structure mapping of molecular complexes by super-resolution microscopy
    NIH/NIGMS R01GM124334May 4, 2018 - Mar 31, 2022
    Role: Principal Investigator
    Multimerized GFP probe for live cell imaging
    NIH/NIBIB R21EB022798Jun 1, 2016 - Mar 31, 2018
    Role: Principal Investigator
    Computational design of Cas9-based molecular imaging reagents
    NIH/NIBIB R21EB021453May 10, 2016 - Feb 28, 2018
    Role: Co-Principal Investigator
    Analyzing genomic elements in live animals by CRISPR imaging
    NIH/NIBIB R33EB019784Sep 25, 2014 - Jun 30, 2017
    Role: Principal Investigator
    The Role of Dscam Dock and Cdc42 Effectors in Prosophilia Neuron Dendritogenesis
    NIH/NIMH R21MH101688Mar 15, 2014 - Feb 29, 2016
    Role: Principal Investigator
    Advancing Whole-Organism Screening for Chemical Modifiers of the Brain
    NIH/NINDS R21NS082938Feb 1, 2013 - Jan 31, 2015
    Role: Co-Principal Investigator
    Solving macromolecular complex architecture in situ by super-resolution microscop
    NIH DP2OD008479Sep 30, 2011 - Jun 30, 2016
    Role: Principal Investigator

    Collapse ORNG Applications 
    Collapse Websites
    Collapse In The News

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. Shi X, Garcia G, Wang Y, Reiter JF, Huang B. Deformed alignment of super-resolution images for semi-flexible structures. PLoS One. 2019; 14(3):e0212735. PMID: 30865666.
      View in: PubMed
    2. Chow TT, Shi X, Wei JH, Guan J, Stadler G, Huang B, Blackburn EH. Local enrichment of HP1alpha at telomeres alters their structure and regulation of telomere protection. Nat Commun. 2018 09 04; 9(1):3583. PMID: 30181605.
      View in: PubMed
    3. Percharde M, Lin CJ, Yin Y, Guan J, Peixoto GA, Bulut-Karslioglu A, Biechele S, Huang B, Shen X, Ramalho-Santos M. A LINE1-Nucleolin Partnership Regulates Early Development and ESC Identity. Cell. 2018 Jul 12; 174(2):391-405.e19. PMID: 29937225.
      View in: PubMed
    4. Kelliher MT, Yue Y, Ng A, Kamiyama D, Huang B, Verhey KJ, Wildonger J. Autoinhibition of kinesin-1 is essential to the dendrite-specific localization of Golgi outposts. J Cell Biol. 2018 Jul 02; 217(7):2531-2547. PMID: 29728423.
      View in: PubMed
    5. Sepulveda G, Antkowiak M, Brust-Mascher I, Mahe K, Ou T, Castro NM, Christensen LN, Cheung L, Jiang X, Yoon D, Huang B, Jao LE. Co-translational protein targeting facilitates centrosomal recruitment of PCNT during centrosome maturation in vertebrates. Elife. 2018 Apr 30; 7. PMID: 29708497.
      View in: PubMed
    6. Schnitzbauer J, Wang Y, Zhao S, Bakalar M, Nuwal T, Chen B, Huang B. Correlation analysis framework for localization-based superresolution microscopy. Proc Natl Acad Sci U S A. 2018 03 27; 115(13):3219-3224. PMID: 29531072.
      View in: PubMed
    7. Viswanath P, Radoul M, Izquierdo-Garcia JL, Ong WQ, Luchman HA, Cairncross JG, Huang B, Pieper RO, Phillips JJ, Ronen SM. 2-Hydroxyglutarate-Mediated Autophagy of the Endoplasmic Reticulum Leads to an Unusual Downregulation of Phospholipid Biosynthesis in Mutant IDH1 Gliomas. Cancer Res. 2018 May 01; 78(9):2290-2304. PMID: 29358170.
      View in: PubMed
    8. Citron YR, Fagerstrom CJ, Keszthelyi B, Huang B, Rusan NM, Kelly MJS, Agard DA. The centrosomin CM2 domain is a multi-functional binding domain with distinct cell cycle roles. PLoS One. 2018; 13(1):e0190530. PMID: 29315319.
      View in: PubMed
    9. Feng S, Sekine S, Pessino V, Li H, Leonetti MD, Huang B. Improved split fluorescent proteins for endogenous protein labeling. Nat Commun. 2017 08 29; 8(1):370. PMID: 28851864.
      View in: PubMed
    10. Shi X, Garcia G, Van De Weghe JC, McGorty R, Pazour GJ, Doherty D, Huang B, Reiter JF. Super-resolution microscopy reveals that disruption of ciliary transition-zone architecture causes Joubert syndrome. Nat Cell Biol. 2017 Oct; 19(10):1178-1188. PMID: 28846093.
      View in: PubMed
    11. Mcgorty R, Xie D, Huang B. High-NA open-top selective-plane illumination microscopy for biological imaging. Opt Express. 2017 Jul 24; 25(15):17798-17810. PMID: 28789271.
      View in: PubMed
    12. Pessino V, Citron YR, Feng S, Huang B. Covalent Protein Labeling by SpyTag-SpyCatcher in Fixed Cells for Super-Resolution Microscopy. Chembiochem. 2017 08 04; 18(15):1492-1495. PMID: 28514494.
      View in: PubMed
    13. Irannejad R, Pessino V, Mika D, Huang B, Wedegaertner PB, Conti M, von Zastrow M. Functional selectivity of GPCR-directed drug action through location bias. Nat Chem Biol. 2017 Jul; 13(7):799-806. PMID: 28553949.
      View in: PubMed
    14. Guan J, Liu H, Shi X, Feng S, Huang B. Tracking Multiple Genomic Elements Using Correlative CRISPR Imaging and Sequential DNA FISH. Biophys J. 2017 Mar 28; 112(6):1077-1084. PMID: 28355536.
      View in: PubMed
    15. van Lengerich B, Agnew C, Puchner EM, Huang B, Jura N. EGF and NRG induce phosphorylation of HER3/ERBB3 by EGFR using distinct oligomeric mechanisms. Proc Natl Acad Sci U S A. 2017 04 04; 114(14):E2836-E2845. PMID: 28320942.
      View in: PubMed
    16. Ong WQ, Citron YR, Sekine S, Huang B. Live Cell Imaging of Endogenous mRNA Using RNA-Based Fluorescence "Turn-On" Probe. ACS Chem Biol. 2017 01 20; 12(1):200-205. PMID: 28103687.
      View in: PubMed
    17. Huang B. A microscopic view of the cell. Mol Biol Cell. 2016 11 01; 27(21):3183-3184. PMID: 27799489.
      View in: PubMed
    18. Liu H, Chen S, Huang K, Kim J, Mo H, Iovine R, Gendre J, Pascal P, Li Q, Sun Y, Dong Z, Arkin M, Guo S, Huang B. A High-Content Larval Zebrafish Brain Imaging Method for Small Molecule Drug Discovery. PLoS One. 2016; 11(10):e0164645. PMID: 27732643.
      View in: PubMed
    19. Bai Y, Liu H, Huang B, Wagle M, Guo S. Identification of environmental stressors and validation of light preference as a measure of anxiety in larval zebrafish. BMC Neurosci. 2016 09 15; 17(1):63. PMID: 27633776.
      View in: PubMed
    20. Leonetti MD, Sekine S, Kamiyama D, Weissman JS, Huang B. A scalable strategy for high-throughput GFP tagging of endogenous human proteins. Proc Natl Acad Sci U S A. 2016 06 21; 113(25):E3501-8. PMID: 27274053; PMCID: PMC4922190 [Available on 12/21/16].
    21. Chen B, Guan J, Huang B. Imaging Specific Genomic DNA in Living Cells. Annu Rev Biophys. 2016 07 05; 45:1-23. PMID: 27145877.
      View in: PubMed
    22. Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B. Versatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016 Mar 18; 7:11046. PMID: 26988139; PMCID: PMC4802074.
    23. Bieling P, Li TD, Weichsel J, McGorty R, Jreij P, Huang B, Fletcher DA, Mullins RD. Force Feedback Controls Motor Activity and Mechanical Properties of Self-Assembling Branched Actin Networks. Cell. 2016 Jan 14; 164(1-2):115-127. PMID: 26771487; PMCID: PMC5033619 [Available on 01/14/17].
    24. Chen B, Hu J, Almeida R, Liu H, Balakrishnan S, Covill-Cooke C, Lim WA, Huang B. Expanding the CRISPR imaging toolset with Staphylococcus aureus Cas9 for simultaneous imaging of multiple genomic loci. Nucleic Acids Res. 2016 05 05; 44(8):e75. PMID: 26740581; PMCID: PMC4856973.
    25. Kamiyama D, McGorty R, Kamiyama R, Kim MD, Chiba A, Huang B. Specification of Dendritogenesis Site in Drosophila aCC Motoneuron by Membrane Enrichment of Pak1 through Dscam1. Dev Cell. 2015 Oct 12; 35(1):93-106. PMID: 26460947; PMCID: PMC4626010 [Available on 10/12/16].
    26. Ong WQ, Citron YR, Schnitzbauer J, Kamiyama D, Huang B. Heavy water: a simple solution to increasing the brightness of fluorescent proteins in super-resolution imaging. Chem Commun (Camb). 2015 Sep 11; 51(70):13451-3. PMID: 26226621; PMCID: PMC4544551.
    27. Patton JG, Franklin JL, Weaver AM, Vickers K, Zhang B, Coffey RJ, Ansel KM, Blelloch R, Goga A, Huang B, L'Etoille N, Raffai RL, Lai CP, Krichevsky AM, Mateescu B, Greiner VJ, Hunter C, Voinnet O, McManus MT. Biogenesis, delivery, and function of extracellular RNA. J Extracell Vesicles. 2015; 4:27494. PMID: 26320939; PMCID: PMC4553266.
    28. McGorty R, Liu H, Kamiyama D, Dong Z, Guo S, Huang B. Open-top selective plane illumination microscope for conventionally mounted specimens. Opt Express. 2015 Jun 15; 23(12):16142-53. PMID: 26193587; PMCID: PMC4523553.
    29. Puchner EM, Huang B, Gaub HE. Single molecule techniques--applications in biology. FEBS Lett. 2014 Oct 01; 588(19):3519. PMID: 25157433.
      View in: PubMed
    30. Wang Y, Schnitzbauer J, Hu Z, Li X, Cheng Y, Huang ZL, Huang B. Localization events-based sample drift correction for localization microscopy with redundant cross-correlation algorithm. Opt Express. 2014 Jun 30; 22(13):15982-91. PMID: 24977854; PMCID: PMC4162368.
    31. Sun S, Zhou C, Chen S, Liu J, Yu J, Chilek J, Zhao L, Yu M, Vinluan R, Huang B, Zheng J. Surface-chemistry effect on cellular response of luminescent plasmonic silver nanoparticles. Bioconjug Chem. 2014 Mar 19; 25(3):453-9. PMID: 24559325; PMCID: PMC3983130.
    32. McGorty R, Schnitzbauer J, Zhang W, Huang B. Correction of depth-dependent aberrations in 3D single-molecule localization and super-resolution microscopy. Opt Lett. 2014 Jan 15; 39(2):275-8. PMID: 24562125; PMCID: PMC4030053.
    33. Chen B, Huang B. Imaging genomic elements in living cells using CRISPR/Cas9. Methods Enzymol. 2014; 546:337-54. PMID: 25398348.
      View in: PubMed
    34. Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang B. Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell. 2013 Dec 19; 155(7):1479-91. PMID: 24360272; PMCID: PMC3918502.
    35. Mennella V, Agard DA, Huang B, Pelletier L. Amorphous no more: subdiffraction view of the pericentriolar material architecture. Trends Cell Biol. 2014 Mar; 24(3):188-97. PMID: 24268653; PMCID: PMC3991556.
    36. Puchner EM, Walter JM, Kasper R, Huang B, Lim WA. Counting molecules in single organelles with superresolution microscopy allows tracking of the endosome maturation trajectory. Proc Natl Acad Sci U S A. 2013 Oct 01; 110(40):16015-20. PMID: 24043832; PMCID: PMC3791776.
    37. Schnitzbauer J, McGorty R, Huang B. 4Pi fluorescence detection and 3D particle localization with a single objective. Opt Express. 2013 Aug 26; 21(17):19701-8. PMID: 24105517; PMCID: PMC3796685.
    38. Huang F, Wang X, Ostertag EM, Nuwal T, Huang B, Jan YN, Basbaum AI, Jan LY. TMEM16C facilitates Na(+)-activated K+ currents in rat sensory neurons and regulates pain processing. Nat Neurosci. 2013 Sep; 16(9):1284-90. PMID: 23872594; PMCID: PMC4034143.
    39. McGorty R, Kamiyama D, Huang B. Active Microscope Stabilization in Three Dimensions Using Image Correlation. Opt Nanoscopy. 2013 Apr 18; 2(1). PMID: 24380058.
      View in: PubMed
    40. Irannejad R, Tomshine JC, Tomshine JR, Chevalier M, Mahoney JP, Steyaert J, Rasmussen SG, Sunahara RK, El-Samad H, Huang B, von Zastrow M. Conformational biosensors reveal GPCR signalling from endosomes. Nature. 2013 Mar 28; 495(7442):534-8. PMID: 23515162; PMCID: PMC3835555.
    41. Stender AS, Marchuk K, Liu C, Sander S, Meyer MW, Smith EA, Neupane B, Wang G, Li J, Cheng JX, Huang B, Fang N. Single cell optical imaging and spectroscopy. Chem Rev. 2013 Apr 10; 113(4):2469-527. PMID: 23410134; PMCID: PMC3624028.
    42. Kamiyama D, Huang B. Development in the STORM. Dev Cell. 2012 Dec 11; 23(6):1103-10. PMID: 23237944; PMCID: PMC3523271.
    43. Mennella V, Keszthelyi B, McDonald KL, Chhun B, Kan F, Rogers GC, Huang B, Agard DA. Subdiffraction-resolution fluorescence microscopy reveals a domain of the centrosome critical for pericentriolar material organization. Nat Cell Biol. 2012 Nov; 14(11):1159-68. PMID: 23086239; PMCID: PMC3767400.
    44. Shim SH, Xia C, Zhong G, Babcock HP, Vaughan JC, Huang B, Wang X, Xu C, Bi GQ, Zhuang X. Super-resolution fluorescence imaging of organelles in live cells with photoswitchable membrane probes. Proc Natl Acad Sci U S A. 2012 Aug 28; 109(35):13978-83. PMID: 22891300; PMCID: PMC3435176.
    45. Zhu L, Zhang W, Elnatan D, Huang B. Faster STORM using compressed sensing. Nat Methods. 2012 Apr 22; 9(7):721-3. PMID: 22522657; PMCID: PMC3477591.
    46. Beaudoin GM, Schofield CM, Nuwal T, Zang K, Ullian EM, Huang B, Reichardt LF. Afadin, a Ras/Rap effector that controls cadherin function, promotes spine and excitatory synapse density in the hippocampus. J Neurosci. 2012 Jan 04; 32(1):99-110. PMID: 22219273; PMCID: PMC3305287.
    47. Kasper R, Huang B. SnapShot: Light microscopy. Cell. 2011 Nov 23; 147(5):1198.e1. PMID: 22118471.
      View in: PubMed
    48. Huang B. An in-depth view. Nat Methods. 2011 Apr; 8(4):304-5. PMID: 21451519.
      View in: PubMed
    49. Huang B, Babcock H, Zhuang X. Breaking the diffraction barrier: super-resolution imaging of cells. Cell. 2010 Dec 23; 143(7):1047-58. PMID: 21168201; PMCID: PMC3272504.
    50. Dani A, Huang B, Bergan J, Dulac C, Zhuang X. Superresolution imaging of chemical synapses in the brain. Neuron. 2010 Dec 09; 68(5):843-56. PMID: 21144999; PMCID: PMC3057101.
    51. Wu M, Huang B, Graham M, Raimondi A, Heuser JE, Zhuang X, De Camilli P. Coupling between clathrin-dependent endocytic budding and F-BAR-dependent tubulation in a cell-free system. Nat Cell Biol. 2010 Sep; 12(9):902-8. PMID: 20729836; PMCID: PMC3338250.
    52. Dani A, Huang B. New resolving power for light microscopy: applications to neurobiology. Curr Opin Neurobiol. 2010 Oct; 20(5):648-52. PMID: 20728340.
      View in: PubMed
    53. Huang B. Super-resolution optical microscopy: multiple choices. Curr Opin Chem Biol. 2010 Feb; 14(1):10-4. PMID: 19897404.
      View in: PubMed
    54. Huang B, Bates M, Zhuang X. Super-resolution fluorescence microscopy. Annu Rev Biochem. 2009; 78:993-1016. PMID: 19489737; PMCID: PMC2835776.
    55. Huang B, Jones SA, Brandenburg B, Zhuang X. Whole-cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution. Nat Methods. 2008 Dec; 5(12):1047-52. PMID: 19029906; PMCID: PMC2596623.
    56. Bates M, Huang B, Zhuang X. Super-resolution microscopy by nanoscale localization of photo-switchable fluorescent probes. Curr Opin Chem Biol. 2008 Oct; 12(5):505-14. PMID: 18809508; PMCID: PMC2642911.
    57. Huang B, Wang W, Bates M, Zhuang X. Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy. Science. 2008 Feb 08; 319(5864):810-3. PMID: 18174397; PMCID: PMC2633023.
    58. Kim S, Huang B, Zare RN. Microfluidic separation and capture of analytes for single-molecule spectroscopy. Lab Chip. 2007 Dec; 7(12):1663-5. PMID: 18030384.
      View in: PubMed
    59. Huang B, Kim S, Wu H, Zare RN. Use of a mixture of n-dodecyl-beta-D-maltoside and sodium dodecyl sulfate in poly(dimethylsiloxane) microchips to suppress adhesion and promote separation of proteins. Anal Chem. 2007 Dec 01; 79(23):9145-9. PMID: 17948969.
      View in: PubMed
    60. Bates M, Huang B, Dempsey GT, Zhuang X. Multicolor super-resolution imaging with photo-switchable fluorescent probes. Science. 2007 Sep 21; 317(5845):1749-53. PMID: 17702910; PMCID: PMC2633025.
    61. Whorton MR, Bokoch MP, Rasmussen SG, Huang B, Zare RN, Kobilka B, Sunahara RK. A monomeric G protein-coupled receptor isolated in a high-density lipoprotein particle efficiently activates its G protein. Proc Natl Acad Sci U S A. 2007 May 01; 104(18):7682-7. PMID: 17452637; PMCID: PMC1863461.
    62. Huang B, Yu F, Zare RN. Surface plasmon resonance imaging using a high numerical aperture microscope objective. Anal Chem. 2007 Apr 01; 79(7):2979-83. PMID: 17309232.
      View in: PubMed
    63. Huang B, Wu H, Bhaya D, Grossman A, Granier S, Kobilka BK, Zare RN. Counting low-copy number proteins in a single cell. Science. 2007 Jan 05; 315(5808):81-4. PMID: 17204646.
      View in: PubMed
    64. Luo Y, Huang B, Wu H, Zare RN. Controlling electroosmotic flow in poly(dimethylsiloxane) separation channels by means of prepolymer additives. Anal Chem. 2006 Jul 01; 78(13):4588-92. PMID: 16808469.
      View in: PubMed
    65. Wu H, Huang B, Zare RN. Generation of complex, static solution gradients in microfluidic channels. J Am Chem Soc. 2006 Apr 05; 128(13):4194-5. PMID: 16568971.
      View in: PubMed
    66. Huang B, Wu H, Kim S, Kobilka BK, Zare RN. Phospholipid biotinylation of polydimethylsiloxane (PDMS) for protein immobilization. Lab Chip. 2006 Mar; 6(3):369-73. PMID: 16511619.
      View in: PubMed
    67. Wu H, Huang B, Zare RN. Construction of microfluidic chips using polydimethylsiloxane for adhesive bonding. Lab Chip. 2005 Dec; 5(12):1393-8. PMID: 16286971.
      View in: PubMed
    68. Huang B, Wu H, Kim S, Zare RN. Coating of poly(dimethylsiloxane) with n-dodecyl-beta-D-maltoside to minimize nonspecific protein adsorption. Lab Chip. 2005 Oct; 5(10):1005-7. PMID: 16175253.
      View in: PubMed
    69. Perroud TD, Huang B, Zare RN. Effect of bin time on the photon counting histogram for one-photon excitation. Chemphyschem. 2005 May; 6(5):905-12. PMID: 15884075.
      View in: PubMed
    70. Huang B, Perroud TD, Zare RN. Photon counting histogram: one-photon excitation. Chemphyschem. 2004 Oct 18; 5(10):1523-31. PMID: 15535551.
      View in: PubMed
    71. Perroud TD, Huang B, Wallace MI, Zare RN. Photon counting histogram for one-photon excitation. Chemphyschem. 2003 Oct 17; 4(10):1121-3. PMID: 14596014.
      View in: PubMed
    72. Whelan RJ, Wohland T, Neumann L, Huang B, Kobilka BK, Zare RN. Analysis of biomolecular interactions using a miniaturized surface plasmon resonance sensor. Anal Chem. 2002 Sep 01; 74(17):4570-6. PMID: 12236371.
      View in: PubMed
    73. Ying L, Huang X, Huang B, Xie J, Zhao J, Zhao XS. Fluorescence emission and absorption spectra of single Anabaena sp. strain PCC7120 cells. Photochem Photobiol. 2002 Sep; 76(3):310-3. PMID: 12403452.
      View in: PubMed