Sign in to edit your profile (add interests, mentoring, photo, etc.)

    Charles Craik, PhD

    TitleProfessor
    SchoolUCSF School of Pharmacy
    DepartmentPharmaceutical Chemistry
    Address600 16th Street
    San Francisco CA 94158
    Phone415-476-8146

       Biography 
       Awards and Honors
      National Honor Society1972 - 1975Alden Scholar
      Phi Beta Kappa1974 - 1975Doane Distinguished Scholar
      1975 - 1975Richard Edwin Lee Scholar
      The Amer. Assoc. for the Advan. of Science1978 - 1978Member, The Amer. Chem. Soc
      American Cancer Society 1982 - 1983Postdoctoral Fellow
      American Society of Biological Chemists1983 - 1983Member
      National Institutes of Health1983 - 1984Postdoctoral Fellow
      Science Digest1985 - 1986Top 100 Innovators
      BioTechniques1987 - 1997Editorial Board Member
      DNA1988 - 1988Editorial Board Member
      Protein Engineering1988 - 1988Associate Editor
      Applied Biocatalysis1989 - 1989Editorial Board Member
      Member, The Protein Society1989 - 1990Nominating Committee
      Sigma Xi1991 - 1994Distinguished Lecturer
      National Science Foundation Molecular Biochemistry1993 - 1996Advisory Panel
      Gordon Conference on Proteolytic Enzymes and Inhibitors1996 - 1996Vice Chair
      Gordon Conference on Proteolytic Enzymes and Inhibitors1998 - 1998Chair
      Cold Spring Harbor Meeting on the Biology of Proteolysis1997 - 1997Co-organizer
      Cold Spring Harbor Meeting on the Biology of Proteolysis1999 - 1999Co-organizer
      Cold Spring Harbor Meeting on the Biology of Proteolysis2001 - 2001Co-organizer
      Immunex Corporation (Seattle, WA)2000 - 2000POSH Lecturer
      United States Pharmacopeia2009Council of Experts
      Allegheney College (Meadville, PA)2010 - 2010Lord Lecturer
      American Association for the Advancement of Science2011 - 2011Fellow
      Institute of Organic Chemistry and Biochemistry, Academy of Sciences, Prague, Czech Republic2013 - 2013Institute Medal Lecture and Award

       Overview 
       Overview
      My research focuses on structure-function analysis of proteases and their inhibitors. In my lab we use a combination of genetic, biochemical, and biophysical methods, with particular emphasis on identifying the roles and regulating the activity of proteases associated with infectious diseases, cancer, and development.

      Proteases are directly or indirectly involved in virtually every biological function or dysfunction. The omnipresence of proteases and their natural inhibitors has placed them among the most studied proteins in biochemistry. The research efforts of the lab focus primarily on the structure-function analysis of proteases and their inhibitors using a combination of genetic, biochemical and biophysical methods. Chemical biological approaches are used or are being developed to study the substrate specificity, catalytic mechanism and biological role of proteases and their macromolecular inhibitors. The genes for serine (trypsin, serine collagenase, easter, prostate specific antigen, T-cell specific granzymes, MT-SP1 and 2, KSHV protease), cysteine (cruzain and falcipain) and aspartyl (HIV1 & HIV2 & SIV) proteases and macromolecular inhibitors such as ecotin and single chain antibodies are manipulated and expressed in heterologous expression systems. Targeted, region specific or random substitutions are introduced recombinantly to create variants that provide insight into the structure and activity of the target protein. A particular emphasis of our work is on identifying the roles and regulating the activity of proteases associated with infectious diseases, cancer and development. These studies are providing a better understanding of both the chemical make-up and the biological importance of these critical proteins. This in turn is leading to the development of strategies for regulating proteolytic activity as a means of therapeutic intervention. Further study of these proteins holds promise for better understanding, rapid detection and eventual control of infectious diseases and cancer.


       ORNG Applications 
       Websites
       In The News
       Awarded Grants
       Global Health
       More Info

       Bibliographic 
       Publications
      Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
      List All   |   Timeline
      1. Mahon C, Krogan NJ, Craik CS, Pick E. Cullin e3 ligases and their rewiring by viral factors. Biomolecules. 2014; 4(4):897-930.
        View in: PubMed
      2. Tajon CA, Seo D, Asmussen J, Shah N, Jun YW, Craik CS. Sensitive and selective plasmon ruler nanosensors for monitoring the apoptotic drug response in leukemia. ACS Nano. 2014 Sep 23; 8(9):9199-208.
        View in: PubMed
      3. Gable JE, Lee GM, Jaishankar P, Hearn BR, Waddling CA, Renslo AR, Craik CS. Broad-spectrum allosteric inhibition of herpesvirus proteases. Biochemistry. 2014 Jul 22; 53(28):4648-60.
        View in: PubMed
      4. Grove LM, Southern BD, Jin TH, White KE, Paruchuri S, Harel E, Wei Y, Rahaman SO, Gladson CL, Ding Q, Craik CS, Chapman HA, Olman MA. Urokinase-type Plasminogen Activator Receptor (uPAR) Ligation Induces a Raft-localized Integrin Signaling Switch That Mediates the Hypermotile Phenotype of Fibrotic Fibroblasts. J Biol Chem. 2014 May 2; 289(18):12791-804.
        View in: PubMed
      5. Lebeau AM, Sevillano N, King ML, Duriseti S, Murphy ST, Craik CS, Murphy LL, Vanbrocklin HF. Imaging the urokinase plasminongen activator receptor in preclinical breast cancer models of acquired drug resistance. Theranostics. 2014; 4(3):267-79.
        View in: PubMed
      6. Tajon C, Jun YW, Craik CS. Single-Molecule Sensing of Caspase Activation in Live Cells via Plasmon Coupling Nanotechnology. Methods Enzymol. 2014; 544:271-97.
        View in: PubMed
      7. Asmussen J, Lasater EA, Tajon C, Oses-Prieto J, Jun YW, Taylor BS, Burlingame A, Craik CS, Shah NP. MEK-Dependent Negative Feedback Underlies BCR-ABL-Mediated Oncogene Addiction. Cancer Discov. 2014 Feb; 4(2):200-15.
        View in: PubMed
      8. Du X, Chen NL, Wong A, Craik CS, Brömme D. Elastin degradation by cathepsin v requires two exosites. J Biol Chem. 2013 Nov 29; 288(48):34871-81.
        View in: PubMed
      9. O'Donoghue AJ, Jin Y, Knudsen GM, Perera NC, Jenne DE, Murphy JE, Craik CS, Hermiston TW. Global substrate profiling of proteases in human neutrophil extracellular traps reveals consensus motif predominantly contributed by elastase. PLoS One. 2013; 8(9):e75141.
        View in: PubMed
      10. Corvo I, O'Donoghue AJ, Pastro L, Pi-Denis N, Eroy-Reveles A, Roche L, McKerrow JH, Dalton JP, Craik CS, Caffrey CR, Tort JF. Dissecting the active site of the collagenolytic cathepsin L3 protease of the invasive stage of Fasciola hepatica. PLoS Negl Trop Dis. 2013; 7(7):e2269.
        View in: PubMed
      11. Small JL, O'Donoghue AJ, Boritsch EC, Tsodikov OV, Knudsen GM, Vandal O, Craik CS, Ehrt S. Substrate specificity of MarP, a periplasmic protease required for resistance to acid and oxidative stress in Mycobacterium tuberculosis. J Biol Chem. 2013 May 3; 288(18):12489-99.
        View in: PubMed
      12. Raman K, Trivedi NN, Raymond WW, Ganesan R, Kirchhofer D, Verghese GM, Craik CS, Schneider EL, Nimishakavi S, Caughey GH. Mutational tail loss is an evolutionary mechanism for liberating marapsins and other type I serine proteases from transmembrane anchors. J Biol Chem. 2013 Apr 12; 288(15):10588-98.
        View in: PubMed
      13. LeBeau AM, Duriseti S, Murphy ST, Pepin F, Hann B, Gray JW, VanBrocklin HF, Craik CS. Targeting uPAR with antagonistic recombinant human antibodies in aggressive breast cancer. Cancer Res. 2013 Apr 1; 73(7):2070-81.
        View in: PubMed
      14. LeBeau AM, Lee M, Murphy ST, Hann BC, Warren RS, Delos Santos R, Kurhanewicz J, Hanash SM, VanBrocklin HF, Craik CS. Imaging a functional tumorigenic biomarker in the transformed epithelium. Proc Natl Acad Sci U S A. 2013 Jan 2; 110(1):93-8.
        View in: PubMed
      15. Lee GM, Balouch E, Goetz DH, Lazic A, McKerrow JH, Craik CS. Mapping inhibitor binding modes on an active cysteine protease via nuclear magnetic resonance spectroscopy. Biochemistry. 2012 Dec 18; 51(50):10087-98.
        View in: PubMed
      16. O'Donoghue AJ, Eroy-Reveles AA, Knudsen GM, Ingram J, Zhou M, Statnekov JB, Greninger AL, Hostetter DR, Qu G, Maltby DA, Anderson MO, Derisi JL, McKerrow JH, Burlingame AL, Craik CS. Global identification of peptidase specificity by multiplex substrate profiling. Nat Methods. 2012 Nov; 9(11):1095-100.
        View in: PubMed
      17. Nimishakavi S, Besprozvannaya M, Raymond WW, Craik CS, Gruenert DC, Caughey GH. Activity and inhibition of prostasin and matriptase on apical and basolateral surfaces of human airway epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2012 Jul; 303(2):L97-106.
        View in: PubMed
      18. Sojka D, Franta Z, Frantová H, Bartosová P, Horn M, Váchová J, O'Donoghue AJ, Eroy-Reveles AA, Craik CS, Knudsen GM, Caffrey CR, McKerrow JH, Mares M, Kopácek P. Characterization of gut-associated cathepsin D hemoglobinase from tick Ixodes ricinus (IrCD1). J Biol Chem. 2012 Jun 15; 287(25):21152-63.
        View in: PubMed
      19. Wu S, Avila-Sakar A, Kim J, Booth DS, Greenberg CH, Rossi A, Liao M, Li X, Alian A, Griner SL, Juge N, Yu Y, Mergel CM, Chaparro-Riggers J, Strop P, Tampé R, Edwards RH, Stroud RM, Craik CS, Cheng Y. Fabs enable single particle cryoEM studies of small proteins. Structure. 2012 Apr 4; 20(4):582-92.
        View in: PubMed
      20. Ingram JR, Rafi SB, Eroy-Reveles AA, Ray M, Lambeth L, Hsieh I, Ruelas D, Lim KC, Sakanari J, Craik CS, Jacobson MP, McKerrow JH. Investigation of the proteolytic functions of an expanded cercarial elastase gene family in Schistosoma mansoni. PLoS Negl Trop Dis. 2012; 6(4):e1589.
        View in: PubMed
      21. Norbury LJ, Hung A, Beckham S, Pike RN, Spithill TW, Craik CS, Choe Y, Fecondo JV, Smooker PM. Analysis of Fasciola cathepsin L5 by S2 subsite substitutions and determination of the P1-P4 specificity reveals an unusual preference. Biochimie. 2012 May; 94(5):1119-27.
        View in: PubMed
      22. Craik CS, Shahian T. A screening strategy for trapping the inactive conformer of a dimeric enzyme with a small molecule inhibitor. Methods Mol Biol. 2012; 928:119-31.
        View in: PubMed
      23. Jäger S, Kim DY, Hultquist JF, Shindo K, LaRue RS, Kwon E, Li M, Anderson BD, Yen L, Stanley D, Mahon C, Kane J, Franks-Skiba K, Cimermancic P, Burlingame A, Sali A, Craik CS, Harris RS, Gross JD, Krogan NJ. Vif hijacks CBF-ß to degrade APOBEC3G and promote HIV-1 infection. Nature. 2012 Jan 19; 481(7381):371-5.
        View in: PubMed
      24. Jäger S, Cimermancic P, Gulbahce N, Johnson JR, McGovern KE, Clarke SC, Shales M, Mercenne G, Pache L, Li K, Hernandez H, Jang GM, Roth SL, Akiva E, Marlett J, Stephens M, D'Orso I, Fernandes J, Fahey M, Mahon C, O'Donoghue AJ, Todorovic A, Morris JH, Maltby DA, Alber T, Cagney G, Bushman FD, Young JA, Chanda SK, Sundquist WI, Kortemme T, Hernandez RD, Craik CS, Burlingame A, Sali A, Frankel AD, Krogan NJ. Global landscape of HIV-human protein complexes. Nature. 2012 Jan 19; 481(7381):365-70.
        View in: PubMed
      25. Schneider EL, Lee MS, Baharuddin A, Goetz DH, Farady CJ, Ward M, Wang CI, Craik CS. A reverse binding motif that contributes to specific protease inhibition by antibodies. J Mol Biol. 2012 Jan 27; 415(4):699-715.
        View in: PubMed
      26. Kim J, Stroud RM, Craik CS. Rapid identification of recombinant Fabs that bind to membrane proteins. Methods. 2011 Dec; 55(4):303-9.
        View in: PubMed
      27. Ray M, Hostetter DR, Loeb CR, Simko J, Craik CS. Inhibition of Granzyme B by PI-9 protects prostate cancer cells from apoptosis. Prostate. 2012 Jun 1; 72(8):846-55.
        View in: PubMed
      28. de Poot SA, Westgeest M, Hostetter DR, Van Damme P, Plasman K, Demeyer K, Broekhuizen R, Gevaert K, Craik CS, Bovenschen N. Human and mouse granzyme M display divergent and species-specific substrate specificities. Biochem J. 2011 Aug 1; 437(3):431-42.
        View in: PubMed
      29. Zhou Y, Agudelo J, Lu K, Goetz DH, Hansell E, Chen YT, Roush WR, McKerrow J, Craik CS, Amberg SM, Simmons G. Inhibitors of SARS-CoV entry--identification using an internally-controlled dual envelope pseudovirion assay. Antiviral Res. 2011 Nov; 92(2):187-94.
        View in: PubMed
      30. Franta Z, Sojka D, Frantova H, Dvorak J, Horn M, Srba J, Talacko P, Mares M, Schneider E, Craik CS, McKerrow JH, Caffrey CR, Kopacek P. IrCL1 - the haemoglobinolytic cathepsin L of the hard tick, Ixodes ricinus. Int J Parasitol. 2011 Oct; 41(12):1253-62.
        View in: PubMed
      31. Fahey ME, Bennett MJ, Mahon C, Jäger S, Pache L, Kumar D, Shapiro A, Rao K, Chanda SK, Craik CS, Frankel AD, Krogan NJ. GPS-Prot: a web-based visualization platform for integrating host-pathogen interaction data. BMC Bioinformatics. 2011; 12:298.
        View in: PubMed
      32. Lee GM, Shahian T, Baharuddin A, Gable JE, Craik CS. Enzyme inhibition by allosteric capture of an inactive conformation. J Mol Biol. 2011 Sep 2; 411(5):999-1016.
        View in: PubMed
      33. Craik CS, Page MJ, Madison EL. Proteases as therapeutics. Biochem J. 2011 Apr 1; 435(1):1-16.
        View in: PubMed
      34. Brown CM, Ray M, Eroy-Reveles AA, Egea P, Tajon C, Craik CS. Peptide length and leaving-group sterics influence potency of peptide phosphonate protease inhibitors. Chem Biol. 2011 Jan 28; 18(1):48-57.
        View in: PubMed
      35. Farady CJ, Craik CS. Mechanisms of macromolecular protease inhibitors. Chembiochem. 2010 Nov 22; 11(17):2341-6.
        View in: PubMed
      36. Swenerton RK, Zhang S, Sajid M, Medzihradszky KF, Craik CS, Kelly BL, McKerrow JH. The oligopeptidase B of Leishmania regulates parasite enolase and immune evasion. J Biol Chem. 2011 Jan 7; 286(1):429-40.
        View in: PubMed
      37. Na BK, Bae YA, Zo YG, Choe Y, Kim SH, Desai PV, Avery MA, Craik CS, Kim TS, Rosenthal PJ, Kong Y. Biochemical properties of a novel cysteine protease of Plasmodium vivax, vivapain-4. PLoS Negl Trop Dis. 2010; 4(10):e849.
        View in: PubMed
      38. Raymond WW, Trivedi NN, Makarova A, Ray M, Craik CS, Caughey GH. How immune peptidases change specificity: cathepsin G gained tryptic function but lost efficiency during primate evolution. J Immunol. 2010 Nov 1; 185(9):5360-8.
        View in: PubMed
      39. Cruz CE, Fogaça AC, Nakayasu ES, Angeli CB, Belmonte R, Almeida IC, Miranda A, Miranda MT, Tanaka AS, Braz GR, Craik CS, Schneider E, Caffrey CR, Daffre S. Characterization of proteinases from the midgut of Rhipicephalus (Boophilus) microplus involved in the generation of antimicrobial peptides. Parasit Vectors. 2010; 3:63.
        View in: PubMed
      40. Barkan DT, Hostetter DR, Mahrus S, Pieper U, Wells JA, Craik CS, Sali A. Prediction of protease substrates using sequence and structure features. Bioinformatics. 2010 Jul 15; 26(14):1714-22.
        View in: PubMed
      41. Duriseti S, Goetz DH, Hostetter DR, LeBeau AM, Wei Y, Craik CS. Antagonistic anti-urokinase plasminogen activator receptor (uPAR) antibodies significantly inhibit uPAR-mediated cellular signaling and migration. J Biol Chem. 2010 Aug 27; 285(35):26878-88.
        View in: PubMed
      42. He C, Nora GP, Schneider EL, Kerr ID, Hansell E, Hirata K, Gonzalez D, Sajid M, Boyd SE, Hruz P, Cobo ER, Le C, Liu WT, Eckmann L, Dorrestein PC, Houpt ER, Brinen LS, Craik CS, Roush WR, McKerrow J, Reed SL. A novel Entamoeba histolytica cysteine proteinase, EhCP4, is key for invasive amebiasis and a therapeutic target. J Biol Chem. 2010 Jun 11; 285(24):18516-27.
        View in: PubMed
      43. LeBeau AM, Kostova M, Craik CS, Denmeade SR. Prostate-specific antigen: an overlooked candidate for the targeted treatment and selective imaging of prostate cancer. Biol Chem. 2010 Apr; 391(4):333-43.
        View in: PubMed
      44. Stoop AA, Joshi RV, Eggers CT, Craik CS. Analysis of an engineered plasma kallikrein inhibitor and its effect on contact activation. Biol Chem. 2010 Apr; 391(4):425-33.
        View in: PubMed
      45. Ohol YM, Goetz DH, Chan K, Shiloh MU, Craik CS, Cox JS. Mycobacterium tuberculosis MycP1 protease plays a dual role in regulation of ESX-1 secretion and virulence. Cell Host Microbe. 2010 Mar 18; 7(3):210-20.
        View in: PubMed
      46. Sun C, Su KH, Valentine J, Rosa-Bauza YT, Ellman JA, Elboudwarej O, Mukherjee B, Craik CS, Shuman MA, Chen FF, Zhang X. Time-resolved single-step protease activity quantification using nanoplasmonic resonator sensors. ACS Nano. 2010 Feb 23; 4(2):978-84.
        View in: PubMed
      47. Darragh MR, Schneider EL, Lou J, Phojanakong PJ, Farady CJ, Marks JD, Hann BC, Craik CS. Tumor detection by imaging proteolytic activity. Cancer Res. 2010 Feb 15; 70(4):1505-12.
        View in: PubMed
      48. Camerer E, Barker A, Duong DN, Ganesan R, Kataoka H, Cornelissen I, Darragh MR, Hussain A, Zheng YW, Srinivasan Y, Brown C, Xu SM, Regard JB, Lin CY, Craik CS, Kirchhofer D, Coughlin SR. Local protease signaling contributes to neural tube closure in the mouse embryo. Dev Cell. 2010 Jan 19; 18(1):25-38.
        View in: PubMed
      49. Jun YW, Sheikholeslami S, Hostetter DR, Tajon C, Craik CS, Alivisatos AP. Continuous imaging of plasmon rulers in live cells reveals early-stage caspase-3 activation at the single-molecule level. Proc Natl Acad Sci U S A. 2009 Oct 20; 106(42):17735-40.
        View in: PubMed
      50. Sathler PC, Craik CS, Takeuchi T, Zingali RB, Castro HC. Engineering ecotin for identifying proteins with a trypsin fold. Appl Biochem Biotechnol. 2010 Apr; 160(8):2355-65.
        View in: PubMed
      51. Shahian T, Lee GM, Lazic A, Arnold LA, Velusamy P, Roels CM, Guy RK, Craik CS. Inhibition of a viral enzyme by a small-molecule dimer disruptor. Nat Chem Biol. 2009 Sep; 5(9):640-6.
        View in: PubMed
      52. Kerr ID, Lee JH, Farady CJ, Marion R, Rickert M, Sajid M, Pandey KC, Caffrey CR, Legac J, Hansell E, McKerrow JH, Craik CS, Rosenthal PJ, Brinen LS. Vinyl sulfones as antiparasitic agents and a structural basis for drug design. J Biol Chem. 2009 Sep 18; 284(38):25697-703.
        View in: PubMed
      53. McKerrow JH, Doyle PS, Engel JC, Podust LM, Robertson SA, Ferreira R, Saxton T, Arkin M, Kerr ID, Brinen LS, Craik CS. Two approaches to discovering and developing new drugs for Chagas disease. Mem Inst Oswaldo Cruz. 2009 Jul; 104 Suppl 1:263-9.
        View in: PubMed
      54. Mahon CS, O'Donoghue AJ, Goetz DH, Murray PG, Craik CS, Tuohy MG. Characterization of a multimeric, eukaryotic prolyl aminopeptidase: an inducible and highly specific intracellular peptidase from the non-pathogenic fungus Talaromyces emersonii. Microbiology. 2009 Nov; 155(Pt 11):3673-82.
        View in: PubMed
      55. Dvorák J, Mashiyama ST, Sajid M, Braschi S, Delcroix M, Schneider EL, McKerrow WH, Bahgat M, Hansell E, Babbitt PC, Craik CS, McKerrow JH, Caffrey CR. SmCL3, a gastrodermal cysteine protease of the human blood fluke Schistosoma mansoni. PLoS Negl Trop Dis. 2009; 3(6):e449.
        View in: PubMed
      56. Farady CJ, Sellers BD, Jacobson MP, Craik CS. Improving the species cross-reactivity of an antibody using computational design. Bioorg Med Chem Lett. 2009 Jul 15; 19(14):3744-7.
        View in: PubMed
      57. Liz MA, Fleming CE, Nunes AF, Almeida MR, Mar FM, Choe Y, Craik CS, Powers JC, Bogyo M, Sousa MM. Substrate specificity of transthyretin: identification of natural substrates in the nervous system. Biochem J. 2009 Apr 15; 419(2):467-74.
        View in: PubMed
      58. Lee GM, Craik CS. Trapping moving targets with small molecules. Science. 2009 Apr 10; 324(5924):213-5.
        View in: PubMed
      59. Subramanian S, Hardt M, Choe Y, Niles RK, Johansen EB, Legac J, Gut J, Kerr ID, Craik CS, Rosenthal PJ. Hemoglobin cleavage site-specificity of the Plasmodium falciparum cysteine proteases falcipain-2 and falcipain-3. PLoS One. 2009; 4(4):e5156.
        View in: PubMed
      60. Lilla JN, Joshi RV, Craik CS, Werb Z. Active plasma kallikrein localizes to mast cells and regulates epithelial cell apoptosis, adipocyte differentiation, and stromal remodeling during mammary gland involution. J Biol Chem. 2009 May 15; 284(20):13792-803.
        View in: PubMed
      61. Schneider EL, Craik CS. Positional scanning synthetic combinatorial libraries for substrate profiling. Methods Mol Biol. 2009; 539:59-78.
        View in: PubMed
      62. Raorane DA, Lim MD, Chen FF, Craik CS, Majumdar A. Quantitative and label-free technique for measuring protease activity and inhibition using a microfluidic cantilever array. Nano Lett. 2008 Sep; 8(9):2968-74.
        View in: PubMed
      63. O'Brien TC, Mackey ZB, Fetter RD, Choe Y, O'Donoghue AJ, Zhou M, Craik CS, Caffrey CR, McKerrow JH. A parasite cysteine protease is key to host protein degradation and iron acquisition. J Biol Chem. 2008 Oct 24; 283(43):28934-43.
        View in: PubMed
      64. O'Donoghue AJ, Mahon CS, Goetz DH, O'Malley JM, Gallagher DM, Zhou M, Murray PG, Craik CS, Tuohy MG. Inhibition of a secreted glutamic peptidase prevents growth of the fungus Talaromyces emersonii. J Biol Chem. 2008 Oct 24; 283(43):29186-95.
        View in: PubMed
      65. Dolecková K, Kasný M, Mikes L, Cartwright J, Jedelský P, Schneider EL, Dvorák J, Mountford AP, Craik CS, Horák P. The functional expression and characterisation of a cysteine peptidase from the invasive stage of the neuropathogenic schistosome Trichobilharzia regenti. Int J Parasitol. 2009 Jan; 39(2):201-11.
        View in: PubMed
      66. Lucas JJ, Hayes GR, Kalsi HK, Gilbert RO, Choe Y, Craik CS, Singh BN. Characterization of a cysteine protease from Tritrichomonas foetus that induces host-cell apoptosis. Arch Biochem Biophys. 2008 Sep 15; 477(2):239-43.
        View in: PubMed
      67. Debela M, Beaufort N, Magdolen V, Schechter NM, Craik CS, Schmitt M, Bode W, Goettig P. Structures and specificity of the human kallikrein-related peptidases KLK 4, 5, 6, and 7. Biol Chem. 2008 Jun; 389(6):623-32.
        View in: PubMed
      68. Farady CJ, Egea PF, Schneider EL, Darragh MR, Craik CS. Structure of an Fab-protease complex reveals a highly specific non-canonical mechanism of inhibition. J Mol Biol. 2008 Jul 4; 380(2):351-60.
        View in: PubMed
      69. DuBois KN, Abodeely M, Sakanari J, Craik CS, Lee M, McKerrow JH, Sajid M. Identification of the major cysteine protease of Giardia and its role in encystation. J Biol Chem. 2008 Jun 27; 283(26):18024-31.
        View in: PubMed
      70. Lim MD, Craik CS. Using specificity to strategically target proteases. Bioorg Med Chem. 2009 Feb 1; 17(3):1094-100.
        View in: PubMed
      71. Darragh MR, Bhatt AS, Craik CS. MT-SP1 proteolysis and regulation of cell-microenvironment interactions. Front Biosci. 2008; 13:528-39.
        View in: PubMed
      72. Stack CM, Caffrey CR, Donnelly SM, Seshaadri A, Lowther J, Tort JF, Collins PR, Robinson MW, Xu W, McKerrow JH, Craik CS, Geiger SR, Marion R, Brinen LS, Dalton JP. Structural and functional relationships in the virulence-associated cathepsin L proteases of the parasitic liver fluke, Fasciola hepatica. J Biol Chem. 2008 Apr 11; 283(15):9896-908.
        View in: PubMed
      73. Lazic A, Goetz DH, Nomura AM, Marnett AB, Craik CS. Substrate modulation of enzyme activity in the herpesvirus protease family. J Mol Biol. 2007 Nov 2; 373(4):913-23.
        View in: PubMed
      74. Hostetter DR, Loeb CR, Chu F, Craik CS. Hip is a pro-survival substrate of granzyme B. J Biol Chem. 2007 Sep 21; 282(38):27865-74.
        View in: PubMed
      75. Goetz DH, Choe Y, Hansell E, Chen YT, McDowell M, Jonsson CB, Roush WR, McKerrow J, Craik CS. Substrate specificity profiling and identification of a new class of inhibitor for the major protease of the SARS coronavirus. Biochemistry. 2007 Jul 31; 46(30):8744-52.
        View in: PubMed
      76. Liu GL, Rosa-Bauza YT, Salisbury CM, Craik C, Ellman JA, Chen FF, Lee LP. Peptide-nanoparticle hybrid SERS probes for optical detection of protease activity. J Nanosci Nanotechnol. 2007 Jul; 7(7):2323-30.
        View in: PubMed
      77. Meléndez-López SG, Herdman S, Hirata K, Choi MH, Choe Y, Craik C, Caffrey CR, Hansell E, Chávez-Munguía B, Chen YT, Roush WR, McKerrow J, Eckmann L, Guo J, Stanley SL, Reed SL. Use of recombinant Entamoeba histolytica cysteine proteinase 1 to identify a potent inhibitor of amebic invasion in a human colonic model. Eukaryot Cell. 2007 Jul; 6(7):1130-6.
        View in: PubMed
      78. Farady CJ, Sun J, Darragh MR, Miller SM, Craik CS. The mechanism of inhibition of antibody-based inhibitors of membrane-type serine protease 1 (MT-SP1). J Mol Biol. 2007 Jun 15; 369(4):1041-51.
        View in: PubMed
      79. Bhatt AS, Welm A, Farady CJ, Vásquez M, Wilson K, Craik CS. Coordinate expression and functional profiling identify an extracellular proteolytic signaling pathway. Proc Natl Acad Sci U S A. 2007 Apr 3; 104(14):5771-6.
        View in: PubMed
      80. Sojka D, Hajdusek O, Dvorák J, Sajid M, Franta Z, Schneider EL, Craik CS, Vancová M, Buresová V, Bogyo M, Sexton KB, McKerrow JH, Caffrey CR, Kopácek P. IrAE: an asparaginyl endopeptidase (legumain) in the gut of the hard tick Ixodes ricinus. Int J Parasitol. 2007 Jun; 37(7):713-24.
        View in: PubMed
      81. Chu F, Mahrus S, Craik CS, Burlingame AL. Isotope-coded and affinity-tagged cross-linking (ICATXL): an efficient strategy to probe protein interaction surfaces. J Am Chem Soc. 2006 Aug 16; 128(32):10362-3.
        View in: PubMed
      82. Park K, Kuechle MK, Choe Y, Craik CS, Lawrence OT, Presland RB. Expression and characterization of constitutively active human caspase-14. Biochem Biophys Res Commun. 2006 Sep 8; 347(4):941-8.
        View in: PubMed
      83. Loeb CR, Harris JL, Craik CS. Granzyme B proteolyzes receptors important to proliferation and survival, tipping the balance toward apoptosis. J Biol Chem. 2006 Sep 22; 281(38):28326-35.
        View in: PubMed
      84. Lund LR, Green KA, Stoop AA, Ploug M, Almholt K, Lilla J, Nielsen BS, Christensen IJ, Craik CS, Werb Z, Danø K, Rømer J. Plasminogen activation independent of uPA and tPA maintains wound healing in gene-deficient mice. EMBO J. 2006 Jun 21; 25(12):2686-97.
        View in: PubMed
      85. Debela M, Magdolen V, Schechter N, Valachova M, Lottspeich F, Craik CS, Choe Y, Bode W, Goettig P. Specificity profiling of seven human tissue kallikreins reveals individual subsite preferences. J Biol Chem. 2006 Sep 1; 281(35):25678-88.
        View in: PubMed
      86. Castro HC, Monteiro RQ, Assafim M, Loureiro NI, Craik C, Zingali RB. Ecotin modulates thrombin activity through exosite-2 interactions. Int J Biochem Cell Biol. 2006; 38(11):1893-900.
        View in: PubMed
      87. Baird TT, Wright WD, Craik CS. Conversion of trypsin to a functional threonine protease. Protein Sci. 2006 Jun; 15(6):1229-38.
        View in: PubMed
      88. Nomura AM, Marnett AB, Shimba N, Dötsch V, Craik CS. One functional switch mediates reversible and irreversible inactivation of a herpesvirus protease. Biochemistry. 2006 Mar 21; 45(11):3572-9.
        View in: PubMed
      89. Choe Y, Leonetti F, Greenbaum DC, Lecaille F, Bogyo M, Brömme D, Ellman JA, Craik CS. Substrate profiling of cysteine proteases using a combinatorial peptide library identifies functionally unique specificities. J Biol Chem. 2006 May 5; 281(18):12824-32.
        View in: PubMed
      90. Nomura AM, Marnett AB, Shimba N, Dötsch V, Craik CS. Induced structure of a helical switch as a mechanism to regulate enzymatic activity. Nat Struct Mol Biol. 2005 Nov; 12(11):1019-20.
        View in: PubMed
      91. Herter S, Piper DE, Aaron W, Gabriele T, Cutler G, Cao P, Bhatt AS, Choe Y, Craik CS, Walker N, Meininger D, Hoey T, Austin RJ. Hepatocyte growth factor is a preferred in vitro substrate for human hepsin, a membrane-anchored serine protease implicated in prostate and ovarian cancers. Biochem J. 2005 Aug 15; 390(Pt 1):125-36.
        View in: PubMed
      92. Bhatt AS, Erdjument-Bromage H, Tempst P, Craik CS, Moasser MM. Adhesion signaling by a novel mitotic substrate of src kinases. Oncogene. 2005 Aug 11; 24(34):5333-43.
        View in: PubMed
      93. Mahrus S, Craik CS. Selective chemical functional probes of granzymes A and B reveal granzyme B is a major effector of natural killer cell-mediated lysis of target cells. Chem Biol. 2005 May; 12(5):567-77.
        View in: PubMed
      94. Choe Y, Brinen LS, Price MS, Engel JC, Lange M, Grisostomi C, Weston SG, Pallai PV, Cheng H, Hardy LW, Hartsough DS, McMakin M, Tilton RF, Baldino CM, Craik CS. Development of alpha-keto-based inhibitors of cruzain, a cysteine protease implicated in Chagas disease. Bioorg Med Chem. 2005 Mar 15; 13(6):2141-56.
        View in: PubMed
      95. Marnett AB, Craik CS. Papa's got a brand new tag: advances in identification of proteases and their substrates. Trends Biotechnol. 2005 Feb; 23(2):59-64.
        View in: PubMed
      96. Matsumura M, Bhatt AS, Andress D, Clegg N, Takayama TK, Craik CS, Nelson PS. Substrates of the prostate-specific serine protease prostase/KLK4 defined by positional-scanning peptide libraries. Prostate. 2005 Jan 1; 62(1):1-13.
        View in: PubMed
      97. Mahrus S, Kisiel W, Craik CS. Granzyme M is a regulatory protease that inactivates proteinase inhibitor 9, an endogenous inhibitor of granzyme B. J Biol Chem. 2004 Dec 24; 279(52):54275-82.
        View in: PubMed
      98. Shimba N, Kovacs H, Stern AS, Nomura AM, Shimada I, Hoch JC, Craik CS, Dötsch V. Optimization of 13C direct detection NMR methods. J Biomol NMR. 2004 Oct; 30(2):175-9.
        View in: PubMed
      99. Williamson AL, Lecchi P, Turk BE, Choe Y, Hotez PJ, McKerrow JH, Cantley LC, Sajid M, Craik CS, Loukas A. A multi-enzyme cascade of hemoglobin proteolysis in the intestine of blood-feeding hookworms. J Biol Chem. 2004 Aug 20; 279(34):35950-7.
        View in: PubMed
      100. Serber Z, Straub W, Corsini L, Nomura AM, Shimba N, Craik CS, Ortiz de Montellano P, Dötsch V. Methyl groups as probes for proteins and complexes in in-cell NMR experiments. J Am Chem Soc. 2004 Jun 9; 126(22):7119-25.
        View in: PubMed
      101. Ruggles SW, Fletterick RJ, Craik CS. Characterization of structural determinants of granzyme B reveals potent mediators of extended substrate specificity. J Biol Chem. 2004 Jul 16; 279(29):30751-9.
        View in: PubMed
      102. Marnett AB, Nomura AM, Shimba N, Ortiz de Montellano PR, Craik CS. Communication between the active sites and dimer interface of a herpesvirus protease revealed by a transition-state inhibitor. Proc Natl Acad Sci U S A. 2004 May 4; 101(18):6870-5.
        View in: PubMed
      103. Eggers CT, Murray IA, Delmar VA, Day AG, Craik CS. The periplasmic serine protease inhibitor ecotin protects bacteria against neutrophil elastase. Biochem J. 2004 Apr 1; 379(Pt 1):107-18.
        View in: PubMed
      104. Na BK, Shenai BR, Sijwali PS, Choe Y, Pandey KC, Singh A, Craik CS, Rosenthal PJ. Identification and biochemical characterization of vivapains, cysteine proteases of the malaria parasite Plasmodium vivax. Biochem J. 2004 Mar 1; 378(Pt 2):529-38.
        View in: PubMed
      105. Shimba, N. Nomura, A.M., Marnett, A.B. and Craik, C.S. Herpesvirus Protease Inhibition by Dimer Disruption. J. Virol. 2004; 12(78):6657-65 .
      106. Brachyurins. Tsu, C., and Craik, C.S. Handbook of Proteolytic Enzymes . 2004; 2nd Ed.
      107. Stoop AA, Craik CS. Engineering of a macromolecular scaffold to develop specific protease inhibitors. Nat Biotechnol. 2003 Sep; 21(9):1063-8.
        View in: PubMed
      108. Shimba N, Serber Z, Ledwidge R, Miller SM, Craik CS, Dötsch V. Quantitative identification of the protonation state of histidines in vitro and in vivo. Biochemistry. 2003 Aug 5; 42(30):9227-34.
        View in: PubMed
      109. Bell JK, Goetz DH, Mahrus S, Harris JL, Fletterick RJ, Craik CS. The oligomeric structure of human granzyme A is a determinant of its extended substrate specificity. Nat Struct Biol. 2003 Jul; 10(7):527-34.
        View in: PubMed
      110. Raymond WW, Ruggles SW, Craik CS, Caughey GH. Albumin is a substrate of human chymase. Prediction by combinatorial peptide screening and development of a selective inhibitor based on the albumin cleavage site. J Biol Chem. 2003 Sep 5; 278(36):34517-24.
        View in: PubMed
      111. Leiting B, Pryor KD, Wu JK, Marsilio F, Patel RA, Craik CS, Ellman JA, Cummings RT, Thornberry NA. Catalytic properties and inhibition of proline-specific dipeptidyl peptidases II, IV and VII. Biochem J. 2003 Apr 15; 371(Pt 2):525-32.
        View in: PubMed
      112. Mackewicz CE, Craik CS, Levy JA. The CD8+ cell noncytotoxic anti-HIV response can be blocked by protease inhibitors. Proc Natl Acad Sci U S A. 2003 Mar 18; 100(6):3433-8.
        View in: PubMed
      113. Shimba N, Stern AS, Craik CS, Hoch JC, Dötsch V. Elimination of 13Calpha splitting in protein NMR spectra by deconvolution with maximum entropy reconstruction. J Am Chem Soc. 2003 Mar 5; 125(9):2382-3.
        View in: PubMed
      114. Sun J, Pons J, Craik CS. Potent and selective inhibition of membrane-type serine protease 1 by human single-chain antibodies. Biochemistry. 2003 Feb 4; 42(4):892-900.
        View in: PubMed
      115. Bhatt AS, Takeuchi T, Ylstra B, Ginzinger D, Albertson D, Shuman MA, Craik CS. Quantitation of membrane type serine protease 1 (MT-SP1) in transformed and normal cells. Biol Chem. 2003 Feb; 384(2):257-66.
        View in: PubMed
      116. Barrios AM, Craik CS. Scanning the prime-site substrate specificity of proteolytic enzymes: a novel assay based on ligand-enhanced lanthanide ion fluorescence. Bioorg Med Chem Lett. 2002 Dec 16; 12(24):3619-23.
        View in: PubMed
      117. Singh A, Shenai BR, Choe Y, Gut J, Sijwali PS, Craik CS, Rosenthal PJ. Critical role of amino acid 23 in mediating activity and specificity of vinckepain-2, a papain-family cysteine protease of rodent malaria parasites. Biochem J. 2002 Nov 15; 368(Pt 1):273-81.
        View in: PubMed
      118. Wang X, Choe Y, Craik CS, Ellman JA. Design and synthesis of novel inhibitors of gelatinase B. Bioorg Med Chem Lett. 2002 Aug 19; 12(16):2201-4.
        View in: PubMed
      119. Lecaille F, Choe Y, Brandt W, Li Z, Craik CS, Brömme D. Selective inhibition of the collagenolytic activity of human cathepsin K by altering its S2 subsite specificity. Biochemistry. 2002 Jul 2; 41(26):8447-54.
        View in: PubMed
      120. Salter JP, Choe Y, Albrecht H, Franklin C, Lim KC, Craik CS, McKerrow JH. Cercarial elastase is encoded by a functionally conserved gene family across multiple species of schistosomes. J Biol Chem. 2002 Jul 5; 277(27):24618-24.
        View in: PubMed
      121. Mathieu MA, Bogyo M, Caffrey CR, Choe Y, Lee J, Chapman H, Sajid M, Craik CS, McKerrow JH. Substrate specificity of schistosome versus human legumain determined by P1-P3 peptide libraries. Mol Biochem Parasitol. 2002 Apr 30; 121(1):99-105.
        View in: PubMed
      122. Laboissière MC, Young MM, Pinho RG, Todd S, Fletterick RJ, Kuntz I, Craik CS. Computer-assisted mutagenesis of ecotin to engineer its secondary binding site for urokinase inhibition. J Biol Chem. 2002 Jul 19; 277(29):26623-31.
        View in: PubMed
      123. Reiling KK, Endres NF, Dauber DS, Craik CS, Stroud RM. Anisotropic dynamics of the JE-2147-HIV protease complex: drug resistance and thermodynamic binding mode examined in a 1.09 A structure. Biochemistry. 2002 Apr 9; 41(14):4582-94.
        View in: PubMed
      124. Wang B, Brown KC, Lodder M, Craik CS, Hecht SM. Chemically mediated site-specific proteolysis. Alteration of protein-protein interaction. Biochemistry. 2002 Feb 26; 41(8):2805-13.
        View in: PubMed
      125. Maly DJ, Leonetti F, Backes BJ, Dauber DS, Harris JL, Craik CS, Ellman JA. Expedient solid-phase synthesis of fluorogenic protease substrates using the 7-amino-4-carbamoylmethylcoumarin (ACC) fluorophore. J Org Chem. 2002 Feb 8; 67(3):910-5.
        View in: PubMed
      126. Pray TR, Reiling KK, Demirjian BG, Craik CS. Conformational change coupling the dimerization and activation of KSHV protease. Biochemistry. 2002 Feb 5; 41(5):1474-82.
        View in: PubMed
      127. Dauber DS, Ziermann R, Parkin N, Maly DJ, Mahrus S, Harris JL, Ellman JA, Petropoulos C, Craik CS. Altered substrate specificity of drug-resistant human immunodeficiency virus type 1 protease. J Virol. 2002 Feb; 76(3):1359-68.
        View in: PubMed
      128. Person MD, Brown KC, Mahrus S, Craik CS, Burlingame AL. Novel inter-protein cross-link identified in the GGH-ecotin D137Y dimer. Protein Sci. 2001 Aug; 10(8):1549-62.
        View in: PubMed
      129. Harris JL, Niles A, Burdick K, Maffitt M, Backes BJ, Ellman JA, Kuntz I, Haak-Frendscho M, Craik CS. Definition of the extended substrate specificity determinants for beta-tryptases I and II. J Biol Chem. 2001 Sep 14; 276(37):34941-7.
        View in: PubMed
      130. Castro HC, Silva DM, Craik C, Zingali RB. Structural features of a snake venom thrombin-like enzyme: thrombin and trypsin on a single catalytic platform? Biochim Biophys Acta. 2001 Jun 11; 1547(2):183-95.
        View in: PubMed
      131. Eggers CT, Wang SX, Fletterick RJ, Craik CS. The role of ecotin dimerization in protease inhibition. J Mol Biol. 2001 May 18; 308(5):975-91.
        View in: PubMed
      132. Nealon K, Newcomb WW, Pray TR, Craik CS, Brown JC, Kedes DH. Lytic replication of Kaposi's sarcoma-associated herpesvirus results in the formation of multiple capsid species: isolation and molecular characterization of A, B, and C capsids from a gammaherpesvirus. J Virol. 2001 Mar; 75(6):2866-78.
        View in: PubMed
      133. Selvarajan S, Lund LR, Takeuchi T, Craik CS, Werb Z. A plasma kallikrein-dependent plasminogen cascade required for adipocyte differentiation. Nat Cell Biol. 2001 Mar; 3(3):267-75.
        View in: PubMed
      134. Reiling KK, Pray TR, Craik CS, Stroud RM. Functional consequences of the Kaposi's sarcoma-associated herpesvirus protease structure: regulation of activity and dimerization by conserved structural elements. Biochemistry. 2000 Oct 24; 39(42):12796-803.
        View in: PubMed
      135. Waugh SM, Harris JL, Fletterick R, Craik CS. The structure of the pro-apoptotic protease granzyme B reveals the molecular determinants of its specificity. Nat Struct Biol. 2000 Sep; 7(9):762-5.
        View in: PubMed
      136. Takeuchi T, Harris JL, Huang W, Yan KW, Coughlin SR, Craik CS. Cellular localization of membrane-type serine protease 1 and identification of protease-activated receptor-2 and single-chain urokinase-type plasminogen activator as substrates. J Biol Chem. 2000 Aug 25; 275(34):26333-42.
        View in: PubMed
      137. Harris JL, Backes BJ, Leonetti F, Mahrus S, Ellman JA, Craik CS. Rapid and general profiling of protease specificity by using combinatorial fluorogenic substrate libraries. Proc Natl Acad Sci U S A. 2000 Jul 5; 97(14):7754-9.
        View in: PubMed
      138. Gillmor SA, Takeuchi T, Yang SQ, Craik CS, Fletterick RJ. Compromise and accommodation in ecotin, a dimeric macromolecular inhibitor of serine proteases. J Mol Biol. 2000 Jun 16; 299(4):993-1003.
        View in: PubMed
      139. Sambrano GR, Huang W, Faruqi T, Mahrus S, Craik C, Coughlin SR. Cathepsin G activates protease-activated receptor-4 in human platelets. J Biol Chem. 2000 Mar 10; 275(10):6819-23.
        View in: PubMed
      140. Rozzelle JE, Dauber DS, Todd S, Kelley R, Craik CS. Macromolecular inhibitors of HIV-1 protease. Characterization of designed heterodimers. J Biol Chem. 2000 Mar 10; 275(10):7080-6.
        View in: PubMed
      141. Todd S, Anderson C, Jolly DJ, Craik CS. HIV protease as a target for retrovirus vector-mediated gene therapy. Biochim Biophys Acta. 2000 Mar 7; 1477(1-2):168-88.
        View in: PubMed
      142. Backes BJ, Harris JL, Leonetti F, Craik CS, Ellman JA. Synthesis of positional-scanning libraries of fluorogenic peptide substrates to define the extended substrate specificity of plasmin and thrombin. Nat Biotechnol. 2000 Feb; 18(2):187-93.
        View in: PubMed
      143. Todd S, Laboissière MC, Craik CS. Yeast two-hybrid assay for examining human immunodeficiency virus protease heterodimer formation with dominant-negative inhibitors and multidrug-resistant variants. Anal Biochem. 2000 Jan 15; 277(2):247-53.
        View in: PubMed
      144. K.K. Reiling, T.R. Pray, C.S. Craik, R.M. Stroud. Structure: Regulation of Activity and Dimerization by Conserved Structural Elements. Biochemistry. 2000; 24:12796-803 .
      145. B. Wang, M. Lodder, J. Zhou, T.T. Baird, Jr., K.C. Brown, C.S. Craik, S.M. Hecht. J. Am. Chem. Soc. Chemically Mediated Site-Specific Cleavage of Proteins. 2000; 122:7402-7403.
      146. T.T. Baird Jr., B. Wang, M. Lodder, S.M. Hecht and C.S. Craik. Generation of Active Trypsin by Chemical Cleavage. Tetrahedron Letters. 2000; 556:9477-9485 .
      147. Takeuchi T, Shuman MA, Craik CS. Reverse biochemistry: use of macromolecular protease inhibitors to dissect complex biological processes and identify a membrane-type serine protease in epithelial cancer and normal tissue. Proc Natl Acad Sci U S A. 1999 Sep 28; 96(20):11054-61.
        View in: PubMed
      148. Pray TR, Nomura AM, Pennington MW, Craik CS. Auto-inactivation by cleavage within the dimer interface of Kaposi's sarcoma-associated herpesvirus protease. J Mol Biol. 1999 Jun 4; 289(2):197-203.
        View in: PubMed
      149. Harris JL, Peterson EP, Hudig D, Thornberry NA, Craik CS. Definition and redesign of the extended substrate specificity of granzyme B. J Biol Chem. 1998 Oct 16; 273(42):27364-73.
        View in: PubMed
      150. Yang SQ, Craik CS. Engineering bidentate macromolecular inhibitors for trypsin and urokinase-type plasminogen activator. J Mol Biol. 1998 Jun 19; 279(4):1001-11.
        View in: PubMed
      151. Yang SQ, Wang CI, Gillmor SA, Fletterick RJ, Craik CS. Ecotin: a serine protease inhibitor with two distinct and interacting binding sites. J Mol Biol. 1998 Jun 19; 279(4):945-57.
        View in: PubMed
      152. Brown KC, Yu Z, Burlingame AL, Craik CS. Determining protein-protein interactions by oxidative cross-linking of a glycine-glycine-histidine fusion protein. Biochemistry. 1998 Mar 31; 37(13):4397-406.
        View in: PubMed
      153. Rose RB, Craik CS, Stroud RM. Domain flexibility in retroviral proteases: structural implications for drug resistant mutations. Biochemistry. 1998 Feb 24; 37(8):2607-21.
        View in: PubMed
      154. Harris JL, Craik CS. Engineering enzyme specificity. Curr Opin Chem Biol. 1998 Feb; 2(1):127-32.
        View in: PubMed
      155. Craik CS, Babé LM, Dauber D, McPhee F, Rosé J, Unal A. Engineering catalytically defective forms of HIV protease to modulate its activity. Adv Exp Med Biol. 1998; 436:31-9.
        View in: PubMed
      156. Dauber DS, McPhee F, Unal A, Craik CS. Optimization of a macromolecular inhibitor of HIV-1 protease. Adv Exp Med Biol. 1998; 436:65-70.
        View in: PubMed
      157. Perona JJ, Craik CS. Evolutionary divergence of substrate specificity within the chymotrypsin-like serine protease fold. J Biol Chem. 1997 Nov 28; 272(48):29987-90.
        View in: PubMed
      158. Babé LM, Craik CS. Viral proteases: evolution of diverse structural motifs to optimize function. Cell. 1997 Nov 14; 91(4):427-30.
        View in: PubMed
      159. Caldera PS, Yu Z, Knegtel RM, McPhee F, Burlingame AL, Craik CS, Kuntz ID, Ortiz de Montellano PR. Alkylation of a catalytic aspartate group of the SIV protease by an epoxide inhibitor. Bioorg Med Chem. 1997 Nov; 5(11):2019-27.
        View in: PubMed
      160. Unal A, Pray TR, Lagunoff M, Pennington MW, Ganem D, Craik CS. The protease and the assembly protein of Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8). J Virol. 1997 Sep; 71(9):7030-8.
        View in: PubMed
      161. Gillmor SA, Craik CS, Fletterick RJ. Structural determinants of specificity in the cysteine protease cruzain. Protein Sci. 1997 Aug; 6(8):1603-11.
        View in: PubMed
      162. Rutenber EE, De Voss JJ, Hoffman L, Stroud RM, Lee KH, Alvarez J, McPhee F, Craik C, Ortiz de Montellano PR. The discovery, characterization and crystallographically determined binding mode of an FMOC-containing inhibitor of HIV-1 protease. Bioorg Med Chem. 1997 Jul; 5(7):1311-20.
        View in: PubMed
      163. Ewoldt GR, Smyth MJ, Darcy PK, Harris JL, Craik CS, Horowitz B, Woodard SL, Powers JC, Hudig D. P-4 and RNKP-7, new granzyme-like serine proteases expressed in activated rat lymphocytes. J Immunol. 1997 May 15; 158(10):4574-83.
        View in: PubMed
      164. Perona JJ, Tsu CA, Craik CS, Fletterick RJ. Crystal structure of an ecotin-collagenase complex suggests a model for recognition and cleavage of the collagen triple helix. Biochemistry. 1997 May 6; 36(18):5381-92.
        View in: PubMed
      165. Tsu CA, Perona JJ, Fletterick RJ, Craik CS. Structural basis for the broad substrate specificity of fiddler crab collagenolytic serine protease 1. Biochemistry. 1997 May 6; 36(18):5393-401.
        View in: PubMed
      166. Babé LM, Unal A, Craik CS. Obstruction of HIV-1 particle release by interferon-alpha occurs before viral protease processing and is independent of envelope glycoprotein. J Interferon Cytokine Res. 1997 May; 17(5):287-93.
        View in: PubMed
      167. Kågedahl M, Swaan PW, Redemann CT, Tang M, Craik CS, Szoka FC, Oie S. Use of the intestinal bile acid transporter for the uptake of cholic acid conjugates with HIV-1 protease inhibitory activity. Pharm Res. 1997 Feb; 14(2):176-80.
        View in: PubMed
      168. C.S. Craik & R.J. Fletterick. The Marriage of Protein Structure and Pharmaceutical Discovery. Biotech. & Appl. Biochem. 26 . 1997; 125-126 .
      169. McPhee F, Caldera PS, Bemis GW, McDonagh AF, Kuntz ID, Craik CS. Bile pigments as HIV-1 protease inhibitors and their effects on HIV-1 viral maturation and infectivity in vitro. Biochem J. 1996 Dec 1; 320 ( Pt 2):681-6.
        View in: PubMed
      170. Junker U, Escaich S, Plavec I, Baker J, McPhee F, Rose JR, Craik CS, Böhnlein E. Intracellular expression of human immunodeficiency virus type 1 (HIV-1) protease variants inhibits replication of wild-type and protease inhibitor-resistant HIV-1 strains in human T-cell lines. J Virol. 1996 Nov; 70(11):7765-72.
        View in: PubMed
      171. McPhee F, Good AC, Kuntz ID, Craik CS. Engineering human immunodeficiency virus 1 protease heterodimers as macromolecular inhibitors of viral maturation. Proc Natl Acad Sci U S A. 1996 Oct 15; 93(21):11477-81.
        View in: PubMed
      172. Rose RB, Craik CS, Douglas NL, Stroud RM. Three-dimensional structures of HIV-1 and SIV protease product complexes. Biochemistry. 1996 Oct 1; 35(39):12933-44.
        View in: PubMed
      173. Brinen LS, Willett WS, Craik CS, Fletterick RJ. X-ray structures of a designed binding site in trypsin show metal-dependent geometry. Biochemistry. 1996 May 14; 35(19):5999-6009.
        View in: PubMed
      174. Tsu CA, Craik CS. Substrate recognition by recombinant serine collagenase 1 from Uca pugilator. J Biol Chem. 1996 May 10; 271(19):11563-70.
        View in: PubMed
      175. Gudmundsdóttir E, Spilliaert R, Yang Q, Craik CS, Bjarnason JB, Gudmundsdóttir A. Isolation and characterization of two cDNAs from Atlantic cod encoding two distinct psychrophilic elastases. Comp Biochem Physiol B Biochem Mol Biol. 1996 Apr; 113(4):795-801.
        View in: PubMed
      176. Z. Yu, P. Caldera, F. McPhee, J. DeVoss, P.R. Jones, A. Burlingame, I. Kuntz, C.S. Craik & P.R. Ortiz de Montellano. Irreversible Inhibition of the HIV-1 Protease: Targeting Alkylating Agents to the Catalytic Aspartate Groups. J. Amer. Chem. Soc. 118. 1996; 5846-5856.
      177. W.S. Willett, L.S. Brinen, R.J. Fletterick & C.S. Craik. Delocalizing Trypsin Specificity with Metal-Activation. Biochem; 35. 1996; 5992-5998.
      178. Wang CI, Yang Q, Craik CS. Phage display of proteases and macromolecular inhibitors. Methods Enzymol. 1996; 267:52-68.
        View in: PubMed
      179. S. Halfon & C.S. Craik. Regulation of Proteolytic Activity by Engineered Tridentate Metal Binding Loops. J. Am. Chem. Soc. 118. 1996; 1227-1228 .
      180. E.E. Rutenber, F. McPhee, A.P. Kaplan, S.L. Gallion, J.C. Hogan Jr., C.S. Craik & R.M. Stroud. A New Class of HIV1 Protease Inhibitor: The Crystallographic Structure, Inhibition and Chemical Synthesis of an Aminimide Peptide Isostere. Bioorg.& Med. Chem. 4. 1996; 1545-1558 .
      181. R. Li, X. Chen, B. Gong, Z. Li, E. Davidson, G. Kurzban, R.E. Miller, E.O. Nuzman, J.H. McKerrow, R.J. Fletterick, S.A. Gillmore, C.S. Craik, I.D. Kuntz, F.E. Cohen & G.L. Kenyon. Structure-based Design of Parasitic Protease Inhibitors. Bioorg. & Med. Chem. 4 . 1996; 1421-1427.
      182. Babé LM, Rosé J, Craik CS. Trans-dominant inhibitory human immunodeficiency virus type 1 protease monomers prevent protease activation and virion maturation. Proc Natl Acad Sci U S A. 1995 Oct 24; 92(22):10069-73.
        View in: PubMed
      183. Liu SH, Wong ML, Craik CS, Brodsky FM. Regulation of clathrin assembly and trimerization defined using recombinant triskelion hubs. Cell. 1995 Oct 20; 83(2):257-67.
        View in: PubMed
      184. Corey DR, Willett WS, Coombs GS, Craik CS. Trypsin specificity increased through substrate-assisted catalysis. Biochemistry. 1995 Sep 12; 34(36):11521-7.
        View in: PubMed
      185. Wang CI, Yang Q, Craik CS. Isolation of a high affinity inhibitor of urokinase-type plasminogen activator by phage display of ecotin. J Biol Chem. 1995 May 19; 270(20):12250-6.
        View in: PubMed
      186. Rosé JR, Babé LM, Craik CS. Defining the level of human immunodeficiency virus type 1 (HIV-1) protease activity required for HIV-1 particle maturation and infectivity. J Virol. 1995 May; 69(5):2751-8.
        View in: PubMed
      187. McGrath ME, Eakin AE, Engel JC, McKerrow JH, Craik CS, Fletterick RJ. The crystal structure of cruzain: a therapeutic target for Chagas' disease. J Mol Biol. 1995 Mar 24; 247(2):251-9.
        View in: PubMed
      188. Sloane DL, Leung R, Barnett J, Craik CS, Sigal E. Conversion of human 15-lipoxygenase to an efficient 12-lipoxygenase: the side-chain geometry of amino acids 417 and 418 determine positional specificity. Protein Eng. 1995 Mar; 8(3):275-82.
        View in: PubMed
      189. Perona JJ, Craik CS. Structural basis of substrate specificity in the serine proteases. Protein Sci. 1995 Mar; 4(3):337-60.
        View in: PubMed
      190. Willett WS, Gillmor SA, Perona JJ, Fletterick RJ, Craik CS. Engineered metal regulation of trypsin specificity. Biochemistry. 1995 Feb 21; 34(7):2172-80.
        View in: PubMed
      191. A.E. Eakin, J.H. McKerrow & C.S.Craik. A Cysteine Protease is a Target for the Enzyme Structure-based Design of Antiparasitic Drugs. Drug Information Journal 29. 1995; 29:1501S-1517S.
      192. Rosé JR, Craik CS. Structure-assisted design of nonpeptide human immunodeficiency virus-1 protease inhibitors. Am J Respir Crit Care Med. 1994 Dec; 150(6 Pt 2):S176-82.
        View in: PubMed
      193. Babé LM, Craik CS. Constitutive production of nonenveloped human immunodeficiency virus type 1 particles by a mammalian cell line and effects of a protease inhibitor on particle maturation. Antimicrob Agents Chemother. 1994 Oct; 38(10):2430-9.
        View in: PubMed
      194. Guthmundsdóttir A, Oskarsson S, Eakin AE, Craik CS, Bjarnason JB. Atlantic cod cDNA encoding a psychrophilic chymotrypsinogen. Biochim Biophys Acta. 1994 Sep 13; 1219(1):211-4.
        View in: PubMed
      195. Tsu CA, Perona JJ, Schellenberger V, Turck CW, Craik CS. The substrate specificity of Uca pugilator collagenolytic serine protease 1 correlates with the bovine type I collagen cleavage sites. J Biol Chem. 1994 Jul 29; 269(30):19565-72.
        View in: PubMed
      196. Matthews BW, Craik CS, Neurath H. Can small cyclic peptides have the activity and specificity of proteolytic enzymes? Proc Natl Acad Sci U S A. 1994 May 10; 91(10):4103-5.
        View in: PubMed
      197. Salto R, Babé LM, Li J, Rosé JR, Yu Z, Burlingame A, De Voss JJ, Sui Z, Ortiz de Montellano P, Craik CS. In vitro characterization of nonpeptide irreversible inhibitors of HIV proteases. J Biol Chem. 1994 Apr 8; 269(14):10691-8.
        View in: PubMed
      198. Perona JJ, Hedstrom L, Wagner RL, Rutter WJ, Craik CS, Fletterick RJ. Exogenous acetate reconstitutes the enzymatic activity of trypsin Asp189Ser. Biochemistry. 1994 Mar 22; 33(11):3252-9.
        View in: PubMed
      199. De Voss JJ, Sui Z, DeCamp DL, Salto R, Babé LM, Craik CS, Ortiz de Montellano PR. Haloperidol-based irreversible inhibitors of the HIV-1 and HIV-2 proteases. J Med Chem. 1994 Mar 4; 37(5):665-73.
        View in: PubMed
      200. K.L. Lee, F. McPhee, J.J. DeVoss, C.S. Craik & P. Ortiz de Montellano. Nonpeptide HIV Protease Inhibitors. Differential Introduction of Alkylamino Groups into the two Aryl Rings of Haloperidol. J. of Organic Chemistry 59. 1994; 6194-6199 .
      201. Perona JJ, Evnin LB, Craik CS. A genetic selection elucidates structural determinants of arginine versus lysine specificity in trypsin. Gene. 1993 Dec 27; 137(1):121-6.
        View in: PubMed
      202. Rose RB, Rosé JR, Salto R, Craik CS, Stroud RM. Structure of the protease from simian immunodeficiency virus: complex with an irreversible nonpeptide inhibitor. Biochemistry. 1993 Nov 23; 32(46):12498-507.
        View in: PubMed
      203. Gudmundsdóttir A, Gudmundsdóttir E, Oskarsson S, Bjarnason JB, Eakin AK, Craik CS. Isolation and characterization of cDNAs from Atlantic cod encoding two different forms of trypsinogen. Eur J Biochem. 1993 Nov 1; 217(3):1091-7.
        View in: PubMed
      204. Perona JJ, Craik CS, Fletterick RJ. Locating the catalytic water molecule in serine proteases. Science. 1993 Jul 30; 261(5121):620-2.
        View in: PubMed
      205. Corey DR, Shiau AK, Yang Q, Janowski BA, Craik CS. Trypsin display on the surface of bacteriophage. Gene. 1993 Jun 15; 128(1):129-34.
        View in: PubMed
      206. Rosé JR, Salto R, Craik CS. Regulation of autoproteolysis of the HIV-1 and HIV-2 proteases with engineered amino acid substitutions. J Biol Chem. 1993 Jun 5; 268(16):11939-45.
        View in: PubMed
      207. Perona JJ, Tsu CA, Craik CS, Fletterick RJ. Crystal structures of rat anionic trypsin complexed with the protein inhibitors APPI and BPTI. J Mol Biol. 1993 Apr 5; 230(3):919-33.
        View in: PubMed
      208. Eakin AE, McGrath ME, McKerrow JH, Fletterick RJ, Craik CS. Production of crystallizable cruzain, the major cysteine protease from Trypanosoma cruzi. J Biol Chem. 1993 Mar 25; 268(9):6115-8.
        View in: PubMed
      209. McGrath ME, Haymore BL, Summers NL, Craik CS, Fletterick RJ. Structure of an engineered, metal-actuated switch in trypsin. Biochemistry. 1993 Mar 2; 32(8):1914-9.
        View in: PubMed
      210. J.J. Perona, C.A. Tsu, M.E. McGrath, C.S. Craik & R.J. Fletterick. Relocating a Negative Charge in the Binding Pocket of Trypsin. J. Mol. Bio. 230. 1993; 934-949.
      211. D.L. Decamp, S.B. Kahl & C.S. Craik. Boronated Porphyrins—A New Class of HIV Proteinase Inhibitors. Intl. Antiviral News 1. 1993; 19-20 .
      212. E. Rutenber, E.B. Fauman, R.J. Keenan, S. Fong, P.S. Furth, P.R. Ortiz de Montellano, E. Meng, I.D. Kuntz, D.L. DeCamp, R. Salto, J.R. Rosé, C.S. Craik, R.M. Stroud. . J. Bio. Chem. Structure of a Non-Peptide Inhibitor Complexed with HIV-1 Protease: Developing a Cycle of Structure-Based Drug Design. 1993; 15343-15346.
      213. Z. Sui, R. Salto, C.S. Craik, P. Ortiz de Montellano. Inhibition of the HIV1 and the HIV2 Proteases by Curcumin and Curcumin Boron Complexes. Bioorg. & Med. Chem. Let. 1 . 1993; 415-422 .
      214. T.K. Sawyer, J.F. Fisher, J.B. Hester, C.W. Smith, A.G. Tomasselli, W.G. Tarpley, P.S. Burton, J.O. Hui T.J. McQuade, R.A. Conradi, V.S. Bradford, L. Liu, J.H. Kinner, J.Tustin,D.L. Alexander, A.W. Harrison, D.E. Emmert, D.J. Staples, L.I. Maggira, Y.Z. Zhang, R.A. Poorman, BM. Dunn, C. Rao, .E. Scarborough, W.T. Lowther, C.S. Craik, D. DeCamp, J.Moon, W.H. Howe & R.L. Heinrikson. Peptidomimetic Inhibitors of Human Immunodeficiency Virus Protease (HIV-PR): Design, Enzyme Binding and Selectivity, Antiviral Efficacy and Cell Permeability Properties. Bioorg. Med. Chem. Letts 3. 1993; 819-824 .
      215. Z. Sui, J. DeVoss, D. DeCamp, J. Li, C.S. Craik & P.R. Ortiz de Montellano. Synthesis. Synthesis of Haloperidol Ethanedithioketal HIV-1 Protease Inhibitors: Magnesium Chloride Facilitated Addition of Grignard Reagents. 1993; 8:803-808.
      216. Babé LM, Rosé J, Craik CS. Synthetic "interface" peptides alter dimeric assembly of the HIV 1 and 2 proteases. Protein Sci. 1992 Oct; 1(10):1244-53.
        View in: PubMed
      217. DeCamp DL, Babé LM, Salto R, Lucich JL, Koo MS, Kahl SB, Craik CS. Specific inhibition of HIV-1 protease by boronated porphyrins. J Med Chem. 1992 Sep 4; 35(18):3426-8.
        View in: PubMed
      218. Eakin AE, Mills AA, Harth G, McKerrow JH, Craik CS. The sequence, organization, and expression of the major cysteine protease (cruzain) from Trypanosoma cruzi. J Biol Chem. 1992 Apr 15; 267(11):7411-20.
        View in: PubMed
      219. McGrath ME, Vásquez JR, Craik CS, Yang AS, Honig B, Fletterick RJ. Perturbing the polar environment of Asp102 in trypsin: consequences of replacing conserved Ser214. Biochemistry. 1992 Mar 31; 31(12):3059-64.
        View in: PubMed
      220. Erpel T, Hwang P, Craik CS, Fletterick RJ, McGrath ME. Physical map location of the new Escherichia coli gene eco, encoding the serine protease inhibitor ecotin. J Bacteriol. 1992 Mar; 174(5):1704.
        View in: PubMed
      221. Higaki JN, Fletterick RJ, Craik CS. Engineered metalloregulation in enzymes. Trends Biochem Sci. 1992 Mar; 17(3):100-4.
        View in: PubMed
      222. T.K. Sawyer, D.J. Staples, L. Liu, A.G. Tomasselli, J.O. Hui, K. O’Connell, H. Schostarez, J.B. Hester, J. Moon, W.J. Howe, C.W. Smith, D. L. DeCamp, C.S. Craik, B.M. Dunn,W. T. Lowther, J. Harris, R.A. Poorman, A. Wlodawer, M. Jaskolski & R.L. Heinrikson. . Int. J. Pep. Protein Res. HIV Protease (HIV PR) Inhibitor Structure-Activity-Selectivity and Active Site Molecular Modeling of High Affinity Leu [CH(OH)CH2] Val Modified Viral and Nonviral Substrate Analogs. 1992; 274-281 .
      223. D.R. Corey, M.E. McGrath, J.R. Vasquez, R.J. Fletterick & C.S. Craik. An Alternate Geometry for the Catalytic Triad of Serine Proteases. J. Amer. Chem. Soc. 114. 1992; 4906-4907 .
      224. Sloane DL, Leung R, Craik CS, Sigal E. A primary determinant for lipoxygenase positional specificity. Nature. 1991 Nov 14; 354(6349):149-52.
        View in: PubMed
      225. Wilke ME, Higaki JN, Craik CS, Fletterick RJ. Crystallographic analysis of trypsin-G226A. A specificity pocket mutant of rat trypsin with altered binding and catalysis. J Mol Biol. 1991 Jun 5; 219(3):525-32.
        View in: PubMed
      226. Wilke ME, Higaki JN, Craik CS, Fletterick RJ. Crystal structure of rat trypsin-S195C at -150 degrees C. Analysis of low activity of recombinant and semisynthetic thiol proteases. J Mol Biol. 1991 Jun 5; 219(3):511-23.
        View in: PubMed
      227. McGrath ME, Hines WM, Sakanari JA, Fletterick RJ, Craik CS. The sequence and reactive site of ecotin. A general inhibitor of pancreatic serine proteases from Escherichia coli. J Biol Chem. 1991 Apr 5; 266(10):6620-5.
        View in: PubMed
      228. Babé LM, Pichuantes S, Craik CS. Inhibition of HIV protease activity by heterodimer formation. Biochemistry. 1991 Jan 8; 30(1):106-11.
        View in: PubMed
      229. DeCamp DL, Babé LM, Furth P, Ortiz de Montellano P, Kuntz ID, Craik CS. Structure-based inhibition of HIV-1 protease activity and viral infectivity. Adv Exp Med Biol. 1991; 306:489-92.
        View in: PubMed
      230. Babé LM, Craik CS. Time dependent heterodimer formation leads to inhibition of HIV protease activity. Adv Exp Med Biol. 1991; 306:543-7.
        View in: PubMed
      231. Sloane DL, Dixon RA, Craik CS, Sigal E. Expression of cloned human 15-lipoxygenase in eukaryotic and prokaryotic systems. Adv Prostaglandin Thromboxane Leukot Res. 1991; 21A:25-8.
        View in: PubMed
      232. Earnest T, Fauman E, Craik CS, Stroud R. 1.59 A structure of trypsin at 120 K: comparison of low temperature and room temperature structures. Proteins. 1991; 10(3):171-87.
        View in: PubMed
      233. Tomasselli AG, Howe WJ, Hui JO, Sawyer TK, Reardon IM, DeCamp DL, Craik CS, Heinrikson RL. Calcium-free calmodulin is a substrate of proteases from human immunodeficiency viruses 1 and 2. Proteins. 1991; 10(1):1-9.
        View in: PubMed
      234. D.L. Sloane, R. Leung, C.S. Craik & E. Sigal. A Primary Determinant for Lipoxygenase Postional Specificity. Nature 354, 149-152. 1991.
      235. D.L. DeCamp, L.M. Babé, P. Furth, P. Ortiz de Montellano, I.D. Kuntz & C.S. Craik. Adv. Exptl. Med. Bio. Structure-based inhibition of HIV-1 protease activity and viral infectivity. 1991; 306:489-492 .
      236. C.S.Craik. Modus Operandi. Nature . 1991; 353, 469 .
      237. Higaki JN, Haymore BL, Chen S, Fletterick RJ, Craik CS. Regulation of serine protease activity by an engineered metal switch. Biochemistry. 1990 Sep 18; 29(37):8582-6.
        View in: PubMed
      238. Bathurst IC, Moen LK, Lujan MA, Gibson HL, Feucht PH, Pichuantes S, Craik CS, Santi DV, Barr PJ. Characterization of the human immunodeficiency virus type-1 reverse transcriptase enzyme produced in yeast. Biochem Biophys Res Commun. 1990 Sep 14; 171(2):589-95.
        View in: PubMed
      239. DesJarlais RL, Seibel GL, Kuntz ID, Furth PS, Alvarez JC, Ortiz de Montellano PR, DeCamp DL, Babé LM, Craik CS. Structure-based design of nonpeptide inhibitors specific for the human immunodeficiency virus 1 protease. Proc Natl Acad Sci U S A. 1990 Sep; 87(17):6644-8.
        View in: PubMed
      240. Hall MN, Craik C, Hiraoka Y. Homeodomain of yeast repressor alpha 2 contains a nuclear localization signal. Proc Natl Acad Sci U S A. 1990 Sep; 87(18):6954-8.
        View in: PubMed
      241. Pichuantes S, Babé LM, Barr PJ, DeCamp DL, Craik CS. Recombinant HIV2 protease processes HIV1 Pr53gag and analogous junction peptides in vitro. J Biol Chem. 1990 Aug 15; 265(23):13890-8.
        View in: PubMed
      242. Vanderslice P, Ballinger SM, Tam EK, Goldstein SM, Craik CS, Caughey GH. Human mast cell tryptase: multiple cDNAs and genes reveal a multigene serine protease family. Proc Natl Acad Sci U S A. 1990 May; 87(10):3811-5.
        View in: PubMed
      243. Stewart EA, Craik CS, Hake L, Bowcock AM. Human carboxypeptidase A identifies a BglII RFLP and maps to 7q31-qter. Am J Hum Genet. 1990 Apr; 46(4):795-800.
        View in: PubMed
      244. Sigal E, Grunberger D, Highland E, Gross C, Dixon RA, Craik CS. Expression of cloned human reticulocyte 15-lipoxygenase and immunological evidence that 15-lipoxygenases of different cell types are related. J Biol Chem. 1990 Mar 25; 265(9):5113-20.
        View in: PubMed
      245. Eakin AE, Bouvier J, Sakanari JA, Craik CS, McKerrow JH. Amplification and sequencing of genomic DNA fragments encoding cysteine proteases from protozoan parasites. Mol Biochem Parasitol. 1990 Feb; 39(1):1-8.
        View in: PubMed
      246. M. Obukowicz, M. Gustafson, K. Junger, R. Leimgruber, A. Wittwer, T-C. Wun, T. Warren, B. Bishop, K. Mathis, D. McPherson, N. Siegel, M. Jennings, B. Brightwell, J. Diaz-Collier, L. Bell, C.S. Craik & W. Tacon. Secretion of Active Kringle 2-Serine Protease in E. coli. J. Bio. Chem. 29, 9737-9745 . 1990.
      247. E. Stewart, C.S. Craik, L. Hake & A. Bowcock. Human CPA Identifies a BglII RFLP and Maps to 7q31-qter. Amer. J. Hum. Gen. 46, 795 -800 . 1990.
      248. S. Pichuantes, L. Babé, P. Barr, D. DeCamp & C.S. Craik. Recombinant HIV 2 Protease Processes HIV 1 Pr53gag and Analogous Junction Peptides In Vitro. J. Bio. Chem. 265, 13890-13898 . 1990.
      249. A.L. Ferris, A. Hizi, S.D. Showalter, S. Pichuantes, L. Babé & C.S. Craik. Immunologic and Proteolytic Analysis of HIV-2 Reverse Transcriptase Structure. Virology 175. 1990; 456-464 .
      250. Sloane DL, Craik CS, Sigal E. The expression of active human reticulocyte 15-lipoxygenase in bacteria. Biomed Biochim Acta. 1990; 49(2-3):S11-6.
        View in: PubMed
      251. L. Evnin, J. Vasquez & C.S. Craik. Proc. Substrate Specificity of Trypsin Investigated Using a Genetic Selection. Natl. Acad. Sci. USA 87 . 1990; 6659-6663.
      252. McGrath ME, Wilke ME, Higaki JN, Craik CS, Fletterick RJ. Crystal structures of two engineered thiol trypsins. Biochemistry. 1989 Nov 28; 28(24):9264-70.
        View in: PubMed
      253. Sakanari JA, Staunton CE, Eakin AE, Craik CS, McKerrow JH. Serine proteases from nematode and protozoan parasites: isolation of sequence homologs using generic molecular probes. Proc Natl Acad Sci U S A. 1989 Jul; 86(13):4863-7.
        View in: PubMed
      254. Vanderslice P, Craik CS, Nadel JA, Caughey GH. Molecular cloning of dog mast cell tryptase and a related protease: structural evidence of a unique mode of serine protease activation. Biochemistry. 1989 May 16; 28(10):4148-55.
        View in: PubMed
      255. McGrath ME, Fletterick RJ, Craik CS. Rapid preparation of proteins for crystallization trials. Biotechniques. 1989 Mar; 7(3):246-7.
        View in: PubMed
      256. Vasquez JR, Evnin LB, Higaki JN, Craik CS. An expression system for trypsin. J Cell Biochem. 1989 Mar; 39(3):265-76.
        View in: PubMed
      257. Shimada K, Koh CS, Hashimoto T, Shoji S, Yanagisawa N. [A case of aseptic meningoencephalitis with recurrent abnormal behavior, status epilepticus and aphthous stomatitis, improved by acyclovir]. Rinsho Shinkeigaku. 1989 Mar; 29(3):367-70.
        View in: PubMed
      258. Pichuantes S, Babé LM, Barr PJ, Craik CS. Recombinant HIV1 protease secreted by Saccharomyces cerevisiae correctly processes myristylated gag polyprotein. Proteins. 1989; 6(3):324-37.
        View in: PubMed
      259. Sigal E, Craik CS, Dixon RA, Nadel JA. Cloning and expression of human arachidonate 15-lipoxygenase. Trans Assoc Am Physicians. 1989; 102:176-84.
        View in: PubMed
      260. Sigal E, Grunberger D, Craik CS, Caughey GH, Nadel JA. Arachidonate 15-lipoxygenase from human leukocytes: purification and structural homology to other mammalian lipoxygenases. Adv Prostaglandin Thromboxane Leukot Res. 1989; 19:156-9.
        View in: PubMed
      261. J. Higaki L.B. Evnin & C.S. Craik. Introduction of a Cysteine Protease Active Site into Trypsin. Biochem. 28, 9256-9263 . 1989.
      262. A. Eakin, J.N. Higaki, J.H. McKerrow & C.S. Craik. Cysteine or Serine Proteinase. Nature 342,132. 1989.
      263. Sigal E, Craik CS, Highland E, Grunberger D, Costello LL, Dixon RA, Nadel JA. Molecular cloning and primary structure of human 15-lipoxygenase. Biochem Biophys Res Commun. 1988 Dec 15; 157(2):457-64.
        View in: PubMed
      264. Gráf L, Hegyi G, Likó I, Hepp J, Medzihradszky K, Craik CS, Rutter WJ. Structural and functional integrity of specificity and catalytic sites of trypsin. Int J Pept Protein Res. 1988 Dec; 32(6):512-8.
        View in: PubMed
      265. Gardell SJ, Craik CS, Clauser E, Goldsmith EJ, Stewart CB, Graf M, Rutter WJ. A novel rat carboxypeptidase, CPA2: characterization, molecular cloning, and evolutionary implications on substrate specificity in the carboxypeptidase gene family. J Biol Chem. 1988 Nov 25; 263(33):17828-36.
        View in: PubMed
      266. Clauser E, Gardell SJ, Craik CS, MacDonald RJ, Rutter WJ. Structural characterization of the rat carboxypeptidase A1 and B genes. Comparative analysis of the rat carboxypeptidase gene family. J Biol Chem. 1988 Nov 25; 263(33):17837-45.
        View in: PubMed
      267. Powell SK, Orci L, Craik CS, Moore HP. Efficient targeting to storage granules of human proinsulins with altered propeptide domain. J Cell Biol. 1988 Jun; 106(6):1843-51.
        View in: PubMed
      268. Sigal E, Grunberger D, Craik CS, Caughey GH, Nadel JA. Arachidonate 15-lipoxygenase (omega-6 lipoxygenase) from human leukocytes. Purification and structural homology to other mammalian lipoxygenases. J Biol Chem. 1988 Apr 15; 263(11):5328-32.
        View in: PubMed
      269. Sigal E, Grunberger D, Cashman JR, Craik CS, Caughey GH, Nadel JA. Arachidonate 15-lipoxygenase from human eosinophil-enriched leukocytes: partial purification and properties. Biochem Biophys Res Commun. 1988 Jan 15; 150(1):376-83.
        View in: PubMed
      270. Sprang SR, Fletterick RJ, Gráf L, Rutter WJ, Craik CS. Studies of specificity and catalysis in trypsin by structural analysis of site-directed mutants. Crit Rev Biotechnol. 1988; 8(3):225-36.
        View in: PubMed
      271. Evnin LB, Craik CS. Development of an efficient method for generating and screening active trypsin and trypsin variants. Ann N Y Acad Sci. 1988; 542:61-74.
        View in: PubMed
      272. Craik CS, Roczniak S, Largman C, Rutter WJ. The catalytic role of the active site aspartic acid in serine proteases. Science. 1987 Aug 21; 237(4817):909-13.
        View in: PubMed
      273. Sprang S, Standing T, Fletterick RJ, Stroud RM, Finer-Moore J, Xuong NH, Hamlin R, Rutter WJ, Craik CS. The three-dimensional structure of Asn102 mutant of trypsin: role of Asp102 in serine protease catalysis. Science. 1987 Aug 21; 237(4817):905-9.
        View in: PubMed
      274. Fletcher TS, Alhadeff M, Craik CS, Largman C. Isolation and characterization of a cDNA encoding rat cationic trypsinogen. Biochemistry. 1987 Jun 2; 26(11):3081-6.
        View in: PubMed
      275. Graf L, Craik CS, Patthy A, Roczniak S, Fletterick RJ, Rutter WJ. Selective alteration of substrate specificity by replacement of aspartic acid-189 with lysine in the binding pocket of trypsin. Biochemistry. 1987 May 5; 26(9):2616-23.
        View in: PubMed
      276. Craik CS, Roczniak S, Sprang S, Fletterick R, Rutter W. Redesigning trypsin via genetic engineering. J Cell Biochem. 1987 Mar; 33(3):199-211.
        View in: PubMed
      277. Ebina Y, Araki E, Taira M, Shimada F, Mori M, Craik CS, Siddle K, Pierce SB, Roth RA, Rutter WJ. Replacement of lysine residue 1030 in the putative ATP-binding region of the insulin receptor abolishes insulin- and antibody-stimulated glucose uptake and receptor kinase activity. Proc Natl Acad Sci U S A. 1987 Feb; 84(3):704-8.
        View in: PubMed
      278. W.J. Rutter, S.J. Gardell, S. Roczniak, D. Hilvert, S. Sprang, R.J. Fletterick & C.S. Craik. Edited by D. Oxender and C.F. Fox. New York: Alan R. Liss, Inc. Redesigning Proteins via Genetic Engineering. In: Protein Modification and Design, pp. 257-267. 1987.
      279. Higaki JN, Gibson BW, Craik CS. Evolution of catalysis in the serine proteases. Cold Spring Harb Symp Quant Biol. 1987; 52:615-21.
        View in: PubMed
      280. C.S. Craik. Edited by D. Oxender. New York: Alan R. Liss, Inc. Expression and Overproduction of Proteins. In: Protein Structure, Folding and Design 2, pp. 467-476. . 1987.
      281. C.S. Craik, S. Roczniak, S. Sprang, R.J. Fletterick & W.J. Rutter. Edited by D. Cunningham and G. Long. New York: Alan R Liss, Inc. Redesigning Trypsin via Genetic Engineering. In: Proteases in Biological Control and Biotechnology. 1987; 183-195.
      282. T.L. Burgess, C.S. Craik, L. Matsuuchi & R.B. Kelly. In Vitro Mutagenesis of Trypsinogen: The Role of the N-Terminus in Intracellular Protein Targeting to Secretory Granules. J. Cell Bio. 105, 659-668 . 1987.
      283. Bowcock AM, Crandall J, Daneshvar L, Lee GM, Young B, Zunzunegui V, Craik C, Cavalli-Sforza LL, King MC. Genetic analysis of cystic fibrosis: linkage of DNA and classical markers in multiplex families. Am J Hum Genet. 1986 Dec; 39(6):699-706.
        View in: PubMed
      284. S. Gardell, C.S. Craik, D. Hilvert, M. Urdea & W. Rutter. Edited by I. Bertini, C. Luchinat, W. Maret, and M. Zeppezauer. Boston, Massachusetts: Birkhäuser. Probing the Role of Tyrosine 248 in Carboxypeptidase A with Site-Directed Mutagenesis. In: Comparative Analysis of Catalytic Mechanisms of Zinc Enzymes. . 1986.
      285. Gardell SJ, Craik CS, Hilvert D, Urdea MS, Rutter WJ. Site-directed mutagenesis shows that tyrosine 248 of carboxypeptidase A does not play a crucial role in catalysis. Nature. 1985 Oct 10-16; 317(6037):551-5.
        View in: PubMed
      286. Burgess TL, Craik CS, Kelly RB. The exocrine protein trypsinogen is targeted into the secretory granules of an endocrine cell line: studies by gene transfer. J Cell Biol. 1985 Aug; 101(2):639-45.
        View in: PubMed
      287. Craik CS, Largman C, Fletcher T, Roczniak S, Barr PJ, Fletterick R, Rutter WJ. Redesigning trypsin: alteration of substrate specificity. Science. 1985 Apr 19; 228(4697):291-7.
        View in: PubMed
      288. C.S. Craik. Biotechniques. Use of Oligonucleotides for Site-Specific Mutagenesis. 1985; 3:12-19.
      289. Craik CS, Choo QL, Swift GH, Quinto C, MacDonald RJ, Rutter WJ. Structure of two related rat pancreatic trypsin genes. J Biol Chem. 1984 Nov 25; 259(22):14255-64.
        View in: PubMed
      290. Bell GI, Quinto C, Quiroga M, Valenzuela P, Craik CS, Rutter WJ. Isolation and sequence of a rat chymotrypsin B gene. J Biol Chem. 1984 Nov 25; 259(22):14265-70.
        View in: PubMed
      291. Swift GH, Craik CS, Stary SJ, Quinto C, Lahaie RG, Rutter WJ, MacDonald RJ. Structure of the two related elastase genes expressed in the rat pancreas. J Biol Chem. 1984 Nov 25; 259(22):14271-8.
        View in: PubMed
      292. Honey NK, Sakaguchi AY, Lalley PA, Quinto C, MacDonald RJ, Craik C, Bell GI, Rutter WJ, Naylor SL. Chromosomal assignments of genes for trypsin, chymotrypsin B, and elastase in mouse. Somat Cell Mol Genet. 1984 Jul; 10(4):377-83.
        View in: PubMed
      293. Honey NK, Sakaguchi AY, Quinto C, MacDonald RJ, Bell GI, Craik C, Rutter WJ, Naylor SL. Chromosomal assignments of human genes for serine proteases trypsin, chymotrypsin B, and elastase. Somat Cell Mol Genet. 1984 Jul; 10(4):369-76.
        View in: PubMed
      294. Craik CS, Rutter WJ, Fletterick R. Splice junctions: association with variation in protein structure. Science. 1983 Jun 10; 220(4602):1125-9.
        View in: PubMed
      295. Craik CS, Sprang S, Fletterick R, Rutter WJ. Intron-exon splice junctions map at protein surfaces. Nature. 1982 Sep 9; 299(5879):180-2.
        View in: PubMed
      296. C.S. Craik & S. Beychok. Edited by C. Ho. In: Interaction Between Iron Proteins in Oxygen and Electron Transport, Elsevier North Holland Biomedical Press . 1982; 192-197.
      297. Craik CS, Buchman SR, Beychok S. O2 binding properties of the product of the central exon of beta-globin gene. Nature. 1981 May 7; 291(5810):87-90.
        View in: PubMed
      298. Craik CS, Vallette I, Beychok S, Waks M. Refolding defects in hemoglobin Rothschild. J Biol Chem. 1980 Jul 10; 255(13):6219-23.
        View in: PubMed
      299. Craik CS, Buchman SR, Beychok S. Characterization of globin domains: heme binding to the central exon product. Proc Natl Acad Sci U S A. 1980 Mar; 77(3):1384-8.
        View in: PubMed
      Charles's Networks
      Related Concepts
      Derived automatically from this person's publications.
      _
      Co-Authors
      People in Profiles who have published with this person.
      _
      Related Authors
      People who share related concepts with this person.
      _
      Back to TOP